Figure 4

Protection of α1H-/- hippocampal and cortical neurons by either trimethadione or mibefradil. Neurons of E18 knockout mice missing the α1H subunit of the T-type calcium channel were cultured for 7-8 days. (A) On day 8 of culture, the medium was refreshed and the hippocampal neurons given either 0 mM, 0.6 mM TMO, or 1 μM mibefradil (control, n = 24; treatment groups, n = 12 each). After 48 hours, cell death was quantified using an LDH assay. The raw data was used to perform one-way ANOVA. Afterwards the mean LDH values were expressed as % of control. *p ≤ 0.05 and **p ≤ 0.01 compared to the control was significant. (B) Cortical neurons were given either 0 mM, 0.6 mM TMO, or 1 μM mibefradil (control, n = 24; treatment groups, n = 12 each). After 48 hours, cell death was quantified using an LDH assay. The raw data was used to perform one-way ANOVA. Afterwards the mean LDH values were expressed as % of control. *p ≤ 0.05 and **p ≤ 0.01 compared to the control was significant using student's t test.