- Research article
- Open Access
Neuroprotective effects of blockers for T-type calcium channels
© Wildburger et al; licensee BioMed Central Ltd. 2009
- Received: 3 August 2009
- Accepted: 28 October 2009
- Published: 28 October 2009
Cognitive and functional decline with age is correlated with deregulation of intracellular calcium, which can lead to neuronal death in the brain. Previous studies have found protective effects of various calcium channel blockers in pathological conditions. However, little has been done to explore possible protective effects of blockers for T-type calcium channels, which forms a family of FDA approved anti-epileptic drugs. In this study, we found that neurons showed an increase in viability after treatment with either L-type or T-type calcium channel antagonists. The family of low-voltage activated, or T-type calcium channels, comprise of three members (Cav3.1, Cav3.2, and Cav3.3) based on their respective main pore-forming alpha subunits: α1G, α1H, and α1I. Among these three subunits, α1H is highly expressed in hippocampus and certain cortical regions. However, T-type calcium channel blockers can protect neurons derived from α1H-/- mice, suggesting that neuroprotection demonstrated by these drugs is not through the α1H subunit. In addition, blockers for T-type calcium channels were not able to confer any protection to neurons in long-term cultures, while blockers of L-type calcium channels could protect neurons. These data indicate a new function of blockers for T-type calcium channels, and also suggest different mechanisms to regulate neuronal survival by calcium signaling pathways. Thus, our findings have important implications in the development of new treatment for age-related neurodegenerative disorders.
- Calcium Channel
- Hippocampal Culture
Calcium signaling pathways play a vital role in the survival of neurons. With increasing age, calcium homeostasis can be disrupted in the brain, which leads to cognitive and functional decline [1–6]. Thus it raises the possibility of protecting neurons by identifying chemicals able to modulate calcium homeostasis in neurons during aging.
Calcium homeostasis can be regulated by several types of calcium channels, including voltage-gated calcium channels (VGCCs). VGCCs can be divided into two groups: high-voltage activated calcium channels such as L-type calcium channels and low-voltage activated calcium channels such as T-type calcium channels [7, 8]. The family of T-type calcium channels comprise three members (Cav3.1, Cav3.2, and Cav3.3) based on their respective main pore-forming alpha subunits: α1G, α1H, and α1I [9, 10]. T-type calcium channels are predominantly found in neurons [11, 12], but have been found in other cells including smooth muscle myocytes, pacemaker cells of the heart, glial cells, fibroblasts, osteoblasts, retinal cells, and adrenocortical cells [13–15]. L-type channels also have a wide distribution in central nervous system .
Blockers for both L-type and T-type calcium channels have been developed to treat various diseases. Trimethadione (TMO) is a T-type calcium channel blocker approved by the FDA as an anticonvulsant for absence seizures. Interestingly, TMO can also ameliorate noise-induced hearing loss (NIHL) by preserving the outer hair cells  and extend the life span of C. elegans . Another blocker for T-type calcium channels, mibefradil, is a particularly effective inhibitor of the Ca+2 influx mediated by the α1H (Cav3.2) subunit . In previous studies, it has shown to increase rat survival with chronic heart failure  and limit infarct size  with weak inotropic effects [22–24]. Mibefradil can protect neurons under oxygen-glucose deprivation events and post-ischemic conditions . Blockers for L-type calcium channels such as nimodipine have been shown to increase survival after global ischemia , prevent apoptotic and necrotic cell death after transient focal ischemia [27, 28], reduce damage resulting from brain edema , improve patient outcome with severe head injuries, related secondary neuronal damage , and subarachnoid hemorrhage . However, the possible molecular mechanisms for the beneficial effects of T-type and L-type calcium channel blockers are largely unknown, mainly due to complicated in vivo interactions. In this study, we established cell culture models to directly test whether these drugs could preserve neurons in vitro in both long-term and short-term cultures.
Neuroprotection by Nimodine
Neuroprotection by TMO
Neuroprotection by Mibefradil
The α1H subunit is not the molecular target for neuroprotection by blockers for T-type calcium channels
Neuroprotection in long-term cultures
Intracellular calcium increase is an early event triggering neuronal death in age-related neurodegenerative disorders such as Alzheimer's disease [1–6]. The L-type voltage-gated calcium channels have been implicated in playing a role in neuronal death during aging [33, 34]. Though T-type calcium current increases with age , to our knowledge the role of low-voltage gated calcium channels in the neuronal survival has never been studied. Using a well characterized in vitro neuronal culture model, we first show that both blockers of L-type and T-type calcium channels can protect neurons in the dish. Although the α1H subunit is highly expressed in the hippocampus and in regions of the cortex, blockers for T-type calcium channels continued to protect neurons derived from the α1H-/- mice, indicating that the neuroprotection demonstrated by these drugs is not through the α1H subunit. Interestingly, nimodipine, a blocker for L-type calcium channels, can protect neurons in the long-term culture model, while blockers for T-type calcium channels are unable to protect neurons in the same culture model.
It has been suggested that T-type calcium channels contribute to intracellular calcium increase and cell death for both glial and neuronal cells under ischemia conditions [36, 25]. Here, we show that trimethadione and mibefradil provide a very significant protection against neuronal death in the dish. Both drugs are selective inhibitors for T-type calcium channels. The neuroprotective effects of anticonvulsants on organotypic hippocampal cultures subjected to transient ischemia have been reported . One possible explanation for the lack of neuroprotection with 0.9 mM trimethadione (Fig. 2) is an excessive block of calcium channel currents causing a detrimental lowering of intracellular calcium concentration in the cell. Since calcium ions play important roles as second messengers, vesicle fusion and neurotransmitter release, and axon growth cones, any drastic inhibition of Ca+2 currents would also be lethal to neurons [38–44]. It is interesting that ethosuximide, phenobarbital, and phenytoin, reported to be the most neuroprotective anticonvulsants, are also the drugs with most potent T-type calcium current inhibitory activity [45, 46], which suggests that these drugs protect neurons through blocking T-type calcium channels.
In addition, our results show that similar drugs can still protect neurons derived from mice lacking the α1H subunit. The neuroprotection demonstrated by T-type calcium channel antagonists in this case can be due to the presence of the other two subunits (α1G or α1I) in the cultured neurons. Therefore, it would be interesting in the future to test their protective effects in neurons derived from mice lacking either of the other two α1 subunits. In addition, blocking of other ion channels by these two drugs may also be involved in protection of neurons because both of these drugs can bind to other channels with low affinities. For example, mibefradil can block delayed rectifier potassium channels and sodium channels [47, 48].
Presently, there are no effective medications for age-related neurodegeneration. Human population studies have correlated female patients taking calcium channel blocking medication with a better hearing threshold during aging , which suggests that altered calcium regulation might contribute to age-related loss auditory neurons. The "calcium hypothesis of neuronal aging"  has been supported by extensive studies, especially the role of excess calcium influx via L-type voltage-gated calcium channels and age-related changes in calcium intracellular buffering [50, 1]. However, few studies have explored the role of T-type voltage-gated calcium channels in age-related neuronal loss. Therefore, we have tested whether trimethadione could protect neurons in the long-term culture. Surprisingly, no protective effects are observed by trimethadione while a significant protection is observed after blocking L-type calcium channels. Our observation raises the possibility that the survival of neurons depends not only on the level of intracellular calcium but also the source of intracellular calcium. These intracellular sources of calcium include and are not limited to the endoplasmic reticulum via the ryanodine receptors and inositole 1,4,5-triphosphate receptors [51, 52]. In addition, due to the fact that T-type calcium channel blockers can increase C. elegans lifespan, our finding does not exclude the possibility that trimethadione may protect neurons in vivo through its systemic effects .
Currently, mechanisms for neuroprotection by these antiepileptic drugs are unknown. Although there are many differences between in vitro and in vivo conditions, culture models can be useful to dissect molecular pathways underlying age-dependent changes in neurons . Our data from the neuronal cultures suggest that this culture model can be an effective model system to dissect possible protective mechanisms for these drugs. Our findings also encourage potential clinical studies to examine adults taking calcium channel blockers for their cognitive functions with age, including the risk of neurodegenerative disorders.
Our findings suggest that cortical and hippocampal neurons can be protected in vitro by blockers for L-type or T-type calcium channels. The neuroprotection of blockers for T-type calcium channels is not through the α1H subunit. Furthermore, neurons in the long-term culture can be protected only by blocking L-type calcium channels, which suggests different molecular mechanisms for short-term and long-term survival of neurons. Our data provide insights into possible new uses of this family of antiepileptic drugs in protecting neuronal death under pathological conditions.
C57BL/6J and α1H-/- pregnant mice were sacrificed and the fetuses removed at E18. The pups were placed onto a Petri dish with Minimal Essential Medium plus glutamate (MEM) (Gibco, Grand Island, NY). The placenta removed and the pups placed in another Petri dish sterilized with ethanol. The E18 pups were decapitated and the brains removed without extracting the cerebellum and placed in a separate dish with MEM. The brains were sliced in half and the left and right hippocampus removed in addition to the cortex. Hippocampal and cortical sections were each transferred into separate 50 ml polypropylene centrifuge tubes- gamma sterilized (Biolgix, Shawnee Mission, KS) with the MEM using a milliliter pipette. Next, the entire medium was removed from the tube (leaving the cells at the bottom); 5 ml of neurobasal with 2% FBS (Gibco, Grand Island, N.Y.) replaced the old medium. The contents of the tube were mixed using a syringe ten times. Then, using the syringe, the contents were transferred into a 15 ml polypropylene centrifuge tube using a 40 μM nylon filter (BD Falcon, Bedford, MA). The cells were centrifuged at 1,000 rpms for 10 min When finished the medium was removed with a pipette and 5 ml of fresh neurobasal with 2% FBS added. A milliliter pipette was used to mix the contents of the tube five times and then centrifuge (1,000 rpms; 10 min). Once again the medium was removed and replaced with fresh neurobasal (no FBS; 5 ml). 11 ml of neurobasal (no FBS) was placed in a large centrifuge tube and the contents of the small centrifuge tube (total 5 ml) were transferred in to the large tube. Using a micropipette, 150 μl of the cell solution was placed into each well of a 96 tissue culture plate (Zellkultur, Trasadingen, Switzerland). The culture plates were kept in a 37°C incubator, CO2 at 5% (Fisher Scientific).
Preparation of a 96 well culture plate
Culture plates were removed from their wrapping and each well was coated with 50 μl of Poly-D lysine hydrobromide (Sigma, St. Louis, MO). The plates were left to sit for 90 min with Poly-D. Each well was washed out three times using 200 μl of dH20. The plates were sealed with parafilm (Ameican National Can, Greenwich, CT) and refrigerated at 4°C for later use.
Neurobasal medium with 2% FBS
1 ml of B27 supplement (Gibco, Grand Island, NY), 125 μl of L-gluton (200 milli moles) (Sigma, St. Louis MO), 50 μl penicillin (WUSM, St. Louis, MO) 48 ml of neurobasal medium (Gibco, Grand Island, NY), and 1 ml of FBS (100%).
Minimal Essential Medium (MEM)
1 ml of B27 supplement, 125 μl of L-gluton (200 milli moles), 50 μl penicillin, and 49 ml of neurobasal medium.
Monitoring of cell death
To measure cell cytotoxicity, lactate dehydrogenase (LDH) assay was performed. LDH, an enzyme normally found in the cytosol of cells, is released upon damage or death to the cell. 25 μl of medium from the hippocampal or cortical cultures were placed in a 96 well tissue plate with 125 μl LDH buffer (4.53 g KH2PO4 and 11.61 g K2HPO4 in 1 liter, pH 7.4) and 100 μl NADH (Sigma, St. Louis, MO) solution (0.03% NADH in LDH buffer, freshly made) for 10 min. 25 μl of pyruvate solution (0.25% pyruvate in LDH buffer) was added right before the reading using Thermo max microplate reader (Molecular Devices, Sunnyvale, CA), with Soft Max Pro at 340 nm.
Results were expressed as mean ± standard error (SEM). Student's t test and one-way ANOVA test were used in analyzing data for LDH measurements. A p-value less than .05 was regarded as statistically significant.
This work was supported by grants from the National Organization for Hearing Research Foundation, NIH NIA (R01AG024250), and NIH NIDCD (R21DC010489) to JB, and the NSF REU through the Harvey A Friedman Center for Aging and Alzheimer's Disease Research Center at Washington University Medical School. The authors would like to thank Melissa Campbell for reviewing the manuscript and Kevin Ohlemiller for statistical assistance.
- Thibault O, Grant JC, Landfield W: Expansion of the calcium hypothesis of brain aging and Alzheimer's disease: minding the store. Aging Cell. 2007, 6: 307-317. 10.1111/j.1474-9726.2007.00295.x.PubMed CentralView ArticlePubMedGoogle Scholar
- Khachaturian ZS: The role of calcium regulation in brain aging: reexamination of a hypothesis. Aging (Milano). 1989, 1: 17-34.Google Scholar
- Khachaturian ZS: Calcium hypothesis of Alzheimer's disease and brain aging: introduction and overview. Ann NY Acad Sci. 1994, 747: 1-11.View ArticlePubMedGoogle Scholar
- Landfield PW: Aging-related increase in hippocampal calcium channels. Life Sci. 1996, 59: 399-404. 10.1016/0024-3205(96)00318-9.View ArticlePubMedGoogle Scholar
- Landfield PW: "Increased calcium-current" hypothesis of brain aging. Neurobiol Aging. 1987, 8: 346-347. 10.1016/0197-4580(87)90074-1.View ArticlePubMedGoogle Scholar
- Gibson GE: Calcium and the aging nervous system. Neurobiol Aging. 1987, 8: 329-343. 10.1016/0197-4580(87)90072-8.View ArticlePubMedGoogle Scholar
- Catterall WA: Structure and regulation of voltage-gated Ca+2 channels. Ann Rev Cell Dev Biol. 2000, 16: 521-555. 10.1146/annurev.cellbio.16.1.521.View ArticleGoogle Scholar
- Errington AC, Stöhr T, Lees G: Voltage gated ion channels: targets for anticonvulsant drugs. Curr Top Med Chem. 2005, 5: 15-30. 10.2174/1568026053386872.View ArticlePubMedGoogle Scholar
- Perez-Reyes E: Molecular physiology of low-voltage-activated t-type calcium channels. Physiol Rev. 2003, 83: 117-161.View ArticlePubMedGoogle Scholar
- Huguenard JR: Low-threshold calcium currents in central nervous system neurons. Annu Rev Physiol. 1996, 58: 329-348. 10.1146/annurev.ph.58.030196.001553.View ArticlePubMedGoogle Scholar
- Talley E, Cribbs LL, Lee J-H, Daud A, Perez-Reyes E, Bayliss DA: Differential distribution of three members of a gene family encoding low voltage-activated (t-type) calcium channels. J Neurosci. 1999, 19: 1895-1911.PubMedGoogle Scholar
- Heady TN, Gomora JC, Macdonald TL, Perez-Reyes E: Molecular Pharmacology of t-type Ca+2 channels. Jpn J Pharmacol. 2001, 85: 339-350. 10.1254/jjp.85.339.View ArticlePubMedGoogle Scholar
- Ertel SI, Ertel EA, Clozel JP: T-type Ca+2 channels and pharmacological blockade: potential pathophysiological relevance. Cardiovasc Drugs Ther. 1997, 11: 723-739. 10.1023/A:1007706022381.View ArticlePubMedGoogle Scholar
- Yunker MA: Modulation and pharmacology of low voltage-activated T-type calcium channels. J Bioenerg Biomembr. 2003, 35: 577-598. 10.1023/B:JOBB.0000008025.65675.37.View ArticlePubMedGoogle Scholar
- Bringmann A, Schopf S, Reichenbach A: Developmental regulation of calcium-mediated currents in retinal glial (Müller) cells. J Neurophysiol. 2000, 84: 2975-2983.PubMedGoogle Scholar
- Bertolino M, Llinas R: The central role of voltage-activated and receptor-operated calcium channels in neuronal cells. Annu Rev of Pharmacol Toxicol. 1992, 32: 399-421. 10.1146/annurev.pa.32.040192.002151.View ArticleGoogle Scholar
- Shen H, Zhang B, Shin J, Lei D, Du Y, Gao X, Wang Q, Ohlemiller K, Piccirillo J, Bao J: Prophlyactic and therapeutic functions of t-type calcium, blockers against noise-induced hearing loss. Hear Res. 2007, 226: 52-60. 10.1016/j.heares.2006.12.011.PubMed CentralView ArticlePubMedGoogle Scholar
- Evason K, Huang C, Yamben I, Covey D, Kornfeld K: Anticonvulsant medications extend worm life-span. Science. 2005, 307: 258-262. 10.1126/science.1105299.View ArticlePubMedGoogle Scholar
- Perez-Reyes E, Deusen AL, Vitko I: Molecular pharmacology of human Cav3.2 t-type Ca+2 channels: block by antihypertensives, antiarrhythmics, and their analogs. J Pharmacol Exp Ther. 2008, 328: 621-627. 10.1124/jpet.108.145672.PubMed CentralView ArticlePubMedGoogle Scholar
- Mulder P, Richard V, Compagnon P, Henry JP, Lallemand F, Clozel JP, Koen R, Mace B, Thuillez C: Increased survival after long-term treatment with mibefradil, a selective t-channel calcium antagonist, in heart failure. J Am Coll Cardiol. 1997, 29: 416-421. 10.1016/S0735-1097(96)00491-3.View ArticlePubMedGoogle Scholar
- Mocanu MM, Gadgil S, Yellon DM, Baxter GF: Mibefradil, a t-type and l-type calcium blocker, limits infarct size through a glibenclamide-sensitive mechanism. Cardiovasc Drugs Ther. 1999, 13: 115-122. 10.1023/A:1007732025184.View ArticlePubMedGoogle Scholar
- Clozel JP, Veniant M, Osterrieder W: The structurally novel Ca+2 channel blocker Ro 40-which binds to the [3H]desmethoxyverapamil receptor, is devoid of the negative inotropic effects of verapmil in normal and failing rat hearts. Cardiovasc Drug Ther. 5967, 4: 731-736. 10.1007/BF01856562.View ArticleGoogle Scholar
- Osterrieder W, Holck M: In vitro pharmacological profile of Ro 40-a novel Ca+2 channel blocker with potent vasodilation but weak inotropic action. J Cardiovasc Pharmacol. 5967, 13: 754-759.View ArticleGoogle Scholar
- Massie BM: Mibefradil: a selective t-type calcium antagonist. Am J Cardiol. 1997, 80: 231-321. 10.1016/S0002-9149(97)00791-1.View ArticleGoogle Scholar
- Nikonenko I, Bancila M, Bloc A, Muller D, Bijenga P: Inhibition of t-type calcium channels protects neurons from delayed ischemia-induced damage. Mol Pharmacol. 2005, 68: 84-89.PubMedGoogle Scholar
- Zapater P, Moreno J, Horga J: Neuroprotection by the novel calcium antagonist PCA5 nimodipine, and flunarizine, in gerbil global brain ischemia. Brain Res. 0938, 772: 57-62. 10.1016/S0006-8993(97)00838-X.View ArticleGoogle Scholar
- Korenkov AI, Pahnke J, Frei K, Warzok R, Schroeder HW, Frick R, Mulijana L, Piek J, Yonekawa Y, Gaab MR: Treatment with nimodipine or mannitol reduces programmed cell death and infarct size following focal cerebral ischemia. Neurosurg Rev. 2000, 23: 145-150. 10.1007/PL00011946.View ArticlePubMedGoogle Scholar
- Mossakowski MJ, Gadamski R: Influence of the calcium channel blocker on the ischemic changes in sector CA1 pyramidal neurons of Ammon's horn in Mongolian gerbils. Neuropathol Pol. 1987, 25: 439-450.Google Scholar
- Chen LH, Liu LX, Yang YJ, Liu YS, Cao MH: Neuroprotective effects of nimodipine and MK-801 on acute infectious brain edema induced by injection of pertussis bacilli to neocortex of rats. Chin J Traumatol. 2003, 6: 118-123.PubMedGoogle Scholar
- Aslan A, Gurelik M, Cemek M, Goksel HM, Buyukokuroglu ME: Nimodipine can improve cerebral metabolism and outcome in patients with severe head trauma. Pharmacol Res. 2009, 59: 120-124. 10.1016/j.phrs.2008.10.003.View ArticlePubMedGoogle Scholar
- Allen GS, Ahn HS, Preziosi TJ, Battye R, Boone SC, Chou SN, Kelly DL, Weir BK, Crabbe RA, Lavik PJ, Rosenbloom SB, Dorsey FC, Ingram CR, Mellits DE, Bertsch LA, Boisvert DP, Hundley MB, Johnson RK, Strom JA, Transou CR: Cerebral arterial spasm - a controlled trial of nimodipine in patients with subarachnoid hemorrhage. N Engl J Med. 1983, 308: 619-24.View ArticlePubMedGoogle Scholar
- Porter NM, Thibault O, Thibault V, Chen K, Landfield PW: Calcium channel density and hippocampal cell death with age in long-term culture. J Neurosci. 1997, 17: 5629-5639.PubMedGoogle Scholar
- Thibault O, Landfield PW: Increase in single L-type calcium channels in hippocampal neurons during aging. Science. 1996, 272: 1017-1020. 10.1126/science.272.5264.1017.View ArticlePubMedGoogle Scholar
- Thibault O, Hadley R, Landfield PW: Elevated postsynaptic [Ca2+]i and L-type calcium channel activity in aged hippocampal neurons: relationship to impaired synaptic plasticity. J Neurosci. 2001, 21: 9744-9756.PubMedGoogle Scholar
- Murchison D, Griffith WH: Low-voltage activated calcium currents increase in basal forebrain neurons from aged rats. J Neurophysiol. 1995, 74: 876-887.PubMedGoogle Scholar
- Fern R: Intracellular calcium and cell death during ischemia in neonatal rat white matter astrocytes in situ. J Neursci. 1998, 18: 7232-7243.Google Scholar
- Rekling JC: Neuroprotective effects of anticonvulsants in rat hippocampal slice cultures exposed to oxygen/glucose deprivation. Neurosci Lett. 2003, 335: 167-170. 10.1016/S0304-3940(02)01193-X.View ArticlePubMedGoogle Scholar
- Chuang RS, Jaffe H, Cribbs L, Perez-Reyes E, Swartz KJ: Inhibition of T-type voltage-gated calcium channels by a new scorpion toxin. Nat Neurosci. 1988, 1: 668-674.Google Scholar
- Sidach SS, Mintz IM: Kurtoxin, a gating modifier of neuronal high- and low- threshold Ca channels. J Neurosci. 2002, 22: 2023-2034.PubMedGoogle Scholar
- Olamendi-Portugal T, García BI, López-González I, Walt van der J, Dyason K, Ulens C, Tytgat J, Felix R, Darszon A, Possani LD: Two new scorpion toxins that target voltage-gated Ca+2 and Na+ channels. Biochem Biophys Res Commun. 2002, 299: 562-568. 10.1016/S0006-291X(02)02706-7.View ArticlePubMedGoogle Scholar
- Meldolesi J, Pozzan T: Pathways of Ca+2 influx at the plasma membrane: Voltage- receptor- and second messenger-operated channels. Exp Cell Res. 1987, 171: 271-283. 10.1016/0014-4827(87)90161-3.View ArticlePubMedGoogle Scholar
- Katz B, Miledi R: The effect of calcium on acetylcholine release from motor nerve terminals. Proc R Soc Lond. 1965, 161: 496-502. 10.1098/rspb.1965.0017.View ArticlePubMedGoogle Scholar
- Basarsky TA, Parpura V, Haydon PG: Hippocampal synaptogenesis in cell culture: developmental time course of synapse formation, calcium influx, and synaptic protein distribution. J Neurosci. 1994, 14: 6402-6411.PubMedGoogle Scholar
- Mattson MP, Kater SB: Calcium regulation of neurite elongation and growth cone motility. J Neurosci. 1987, 7: 4034-4043.PubMedGoogle Scholar
- Todorovic SM, Lingle CJ: Pharmacological properties of T-type Ca+2 current in adult rat sensory neurons: effects of anticonvulsant and anesthetic agents. J Neurophysiol. 1998, 79: 240-252.PubMedGoogle Scholar
- Todorovic SM, Perez-Reyes E, Lingle CJ: Anticonvulsants but not general anesthetics have differential blocking effects on different T-type current variants. Mol Pharm. 2000, 58: 98-108.Google Scholar
- Liu JH, Bijlenga P, Occhiodoro T, Fischer-Lougheed J, Bader CR, Bernheim L: Mibefradil (Ro 40-5967) inhibits several Ca+2 and K+ currents in human fusion-competent myoblasts. Br J Pharmacol. 1999, 126: 245-250. 10.1038/sj.bjp.0702321.PubMed CentralView ArticlePubMedGoogle Scholar
- McNulty MM, Hanck DA: State-dependent mibefradil block of Na+ channels. Mol Pharmacol. 2004, 66: 1652-1661.PubMedGoogle Scholar
- Mills JH, Matthews LJ, Lee FS, Dubno JR, Schulte BA, Weber PC: Gender-specific effects of drugs on hearing on hearing levels of older persons. Ann N Y Acad Sci. 1999, 884: 381-388. 10.1111/j.1749-6632.1999.tb08656.x.View ArticlePubMedGoogle Scholar
- Foster TC: Calcium homeostasis and modulation of synaptic plasticity in the aged brain. Aging Cell. 2007, 6: 319-325. 10.1111/j.1474-9726.2007.00283.x.View ArticlePubMedGoogle Scholar
- Paschen W, Mengesdorf T: Cellular abnormalities linked to endoplasmic reticulum dysfunction in cerebrovascular disease - therapeutic potential. Pharmacol Ther. 2005, 108: 362-375. 10.1016/j.pharmthera.2005.05.008.View ArticlePubMedGoogle Scholar
- Verkhratsky A: Physiology and pathophysiology of the calcium store in the endoplasmic reticulum of neurons. Physiol Rev. 2005, 85: 201-279. 10.1152/physrev.00004.2004.View ArticlePubMedGoogle Scholar
- Porter NM, Thibault O, Thibault V, Chen K-C, Landfield PW: Calcium channel density and hippocampal cell death with age in long-term culture. J Neurosci. 1997, 17: 5629-5639.PubMedGoogle Scholar
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