Figure 5

Neuroprotection by Nimodipine in long-term culture. Hippocampal neurons from E18 C57BL/6J mice were cultured for 7-8 days in neurobasal medium containing 2% FBS. (A) On the 8 DIV, the medium was replenished and the neurons treated with either 0 or 1 μM nimodipine (control, n = 6; nimodipine, n = 6). Cell death was measured using LDH assay on 15 DIV. Mean values were expressed as % of control ± SEM. *p ≤ 0.05 and **p ≤ 0.01 compared with the control condition. (B) Cortical neurons were treated with either 0 or 1 μM nimodipine (control, n = 6; nimodipine, n = 6). Cell death was measured using LDH assay on 15 DIV. Mean values were expressed as % of control. *p ≤ 0.05 and **p ≤ 0.01 compared with the control condition using student's t test.