Neuromuscular effects of G93A-SOD1 expression in zebrafish
Molecular Neurodegeneration volume 7, Article number: 44 (2012)
Amyotrophic lateral sclerosis (ALS) is a fatal disorder involving the degeneration and loss of motor neurons. The mechanisms of motor neuron loss in ALS are unknown and there are no effective treatments. Defects in the distal axon and at the neuromuscular junction are early events in the disease course, and zebrafish provide a promising in vivo system to examine cellular mechanisms and treatments for these events in ALS pathogenesis.
We demonstrate that transient genetic manipulation of zebrafish to express G93A-SOD1, a mutation associated with familial ALS, results in early defects in motor neuron outgrowth and axonal branching. This is consistent with previous reports on motor neuron axonal defects associated with familial ALS genes following knockdown or mutant protein overexpression. We also demonstrate that upregulation of growth factor signaling is capable of rescuing these early defects, validating the potential of the model for therapeutic discovery. We generated stable transgenic zebrafish lines expressing G93A-SOD1 to further characterize the consequences of G93A-SOD1 expression on neuromuscular pathology and disease progression. Behavioral monitoring reveals evidence of motor dysfunction and decreased activity in transgenic ALS zebrafish. Examination of neuromuscular and neuronal pathology throughout the disease course reveals a loss of neuromuscular junctions and alterations in motor neuron innervations patterns with disease progression. Finally, motor neuron cell loss is evident later in the disease.
This sequence of events reflects the stepwise mechanisms of degeneration in ALS, and provides a novel model for mechanistic discovery and therapeutic development for neuromuscular degeneration in ALS.
Amyotrophic lateral sclerosis (ALS) is an adult-onset, relentlessly progressive, and ultimately lethal neuromuscular disease involving the degeneration and loss of motor neurons (MNs). Effective treatments for ALS have been elusive due to the fact that the mechanisms of MN loss in ALS are unknown. It is likely that multiple cell types within the MN microenvironment and multiple mechanisms contribute to ALS pathogenesis [1, 2]. While MN death is associated with caspase-mediated programmed cell death, neuromuscular junction (NMJ) defects and axonal degeneration are evident early in the disease course before symptom onset and MN loss [3, 4]. Understanding the causes of NMJ and axonal degeneration in ALS and identifying therapies that protect against these early insults represent the best strategy for preventing the irreversible loss of MNs.
The majority of ALS is sporadic with unknown etiology (90-95%); however, the remaining cases are familial (fALS) and have been linked to genetic mutations in genes such as Cu2+/Zn2+ superoxide dismutase (SOD1), Alsin (Als2), dynactin, angiogenin, senataxin, vesicle-associated membrane protein/synaptobrevin-associated membrane protein B, TAR DNA binding protein 43 (TDP43), fused in sarcoma (FUS), optineurin, and ubiquilin 2, or a hexanucleotide repeat expansion in C9ORF72[5–18]. SOD1 mutations are associated with 10-20% of fALS cases and currently more than 150 missense mutations in SOD1 are reported, all of which are associated with a toxic gain of unknown function. Many in vitro and in vivo models expressing mutant SOD1 have been developed to study disease pathogenesis [2, 19]. G93A-SOD1 is the most thoroughly characterized ALS mutation and expression in primary MN cultures results in caspase activation and MN death [20, 21]. In vivo ALS models, including transgenic G93A-SOD1 mice, exhibit MN degeneration, muscle weakness and early lethality, resembling the human ALS disease course [22, 23]. G93A-SOD1 mice also show denervation at NMJs, defects in axonal transport, and axonal degeneration well before the onset of clinical symptoms, which supports a “dying-back” mechanism of MN degeneration [24, 25]. Thus, understanding and targeting the distal degeneration that occurs prior to the irreversible loss of MNs is imperative for developing effective therapies for ALS.
Recently, zebrafish have been gaining momentum as an emerging technology for the study of neurodegenerative diseases [26, 27]. Zebrafish are ideal for the development of novel strategies to understand ALS pathogenesis and screen potential therapies due to the fact that they develop quickly, have large numbers of offspring, are less expensive than rodent models, and they are easily amenable to genetic manipulation. Zebrafish neuromuscular physiology is also similar to humans, neuromuscular development is well characterized and rapid, and many tools exist to closely examine MN and muscle pathology in zebrafish. Furthermore, mechanistic insight into ALS pathogenesis could also be possible through combination with various tissue- and organelle-specific transgenic reporter zebrafish lines available [28–31], and some neurodegenerative disease models have already been developed. Spinal muscular atrophy, a neurodegenerative disease involving MNs, has been successfully modeled in zebrafish [32–34], and transient expression of select ALS mutations has also been reported. Transient overexpression of mutant SOD1 or mutant FUS induces an axonopathy phenotype at 30 hours post-fertilization (hpf) characterized by decreased axon length and aberrant branching [35, 36], as does knockdown of fus, als2, and elp3[35, 37, 38]. Similarly, knockdown of tdp43 or expression of mutant TDP43 in zebrafish embryos results in an axonal phenotype early in development, which is further evidenced in swimming deficits at 48 hpf . While these results reflect an effect of mutant ALS proteins on developing MN axons, the transient expression in these models limits the amount of insight that can be gained regarding the mechanisms of progressive neurodegeneration that is characteristic of ALS.
To investigate ALS disease pathogenesis and progression in zebrafish, we generated novel stable transgenic zebrafish ubiquitously expressing G93A-SOD1. At the onset of this project, no transgenic ALS zebrafish models were established to our knowledge; however, in 2010 the Beattie laboratory reported the generation and characterization of transgenic zebrafish lines ubiquitously expressing zebrafish G93R-sod1. This model exhibits motor deficits, MN degeneration and loss, and effects on survival  that are characteristic of ALS, validating our approach to generate and utilize the transgenic ALS zebrafish model described in this report. Our initial experiments verified the potential of human G93A-SOD1 to induce the previously reported motor axon defects, and we also demonstrated attenuation of these MN axonal defects with a known neuroprotective factor, insulin-like growth factor-I (IGF-I). We then established stable transgenic zebrafish lines expressing GFP-tagged human G93A-SOD1 using Tol2-mediated transgenesis. Characterization of our stable transgenic G93A-SOD1-GFP zebrafish revealed motor deficits that coincide with defects at the NMJ and subsequent alterations in neuromuscular innervation. We also observe MN loss later in the disease course, which supports a sequence of events that resemble human ALS pathogenesis. Overall, this report describes our detailed examination of MN axonal pathology, NMJ integrity, and MN loss in the context of disease onset and progression in a novel in vivo zebrafish ALS model that will be instrumental in future studies of ALS disease mechanisms and therapeutic development.
Transient overexpression of G93A-SOD1 in zebrafish causes motor axon defects
Before generating stable transgenic G93A-SOD1 zebrafish, we first examined developing zebrafish MN axons following transient human G93A-SOD1 expression. Microinjection of G93A-SOD1 RNA (0 – 100 ng/μl), which was generated from a pCS2 vector that enables efficient expression in zebrafish, results in transient overexpression of the protein as confirmed by western blotting (data not shown). SV2 immunohistochemistry (IHC) reveals that G93A-SOD1 expression results in abnormal premature branching of motor axons at 30 hpf compared to controls (Figure 1A, *). We also detect a dose-dependent effect of G93A-SOD1 expression on axon length compared to uninjected control (UIC) embryos, with significant decreases observed at concentrations as low as 10 ng/μl (Figure 1B). Specifically, axon length is decreased by 13.2%, 17.9%, and 19.1% at doses of 10, 50, and 100 ng/μl G93A-SOD1 RNA, respectively. Co-injection of G93A-SOD1 RNA (50 ng/μl) with igf-I RNA (200 ng/μl) demonstrates that these defects are attenuated by IGF-I upregulation (Figure 1C), a known neuroprotective strategy in ALS model systems . Wild-type SOD1 (wtSOD1) expression has no effect on axon length (Figure 1C). IGF-I upregulation was confirmed by assessing downstream activation of Akt and p44/42 mitogen activated protein kinase (MAPK) signaling and SOD1 expression was confirmed for all clutches using western blotting (data not shown). Together, these findings demonstrate that expression of mutant human SOD1 in zebrafish results in a motor axon phenotype that is amenable to therapeutic intervention, validating the potential usefulness of zebrafish as a model for investigating ALS pathogenesis and drug discovery.
Generation of stable transgenic G93A-SOD1 zebrafish lines
Stable transgenic ALS zebrafish lines were successfully generated for these studies using Tol2-mediated transgenesis. Coinjection of pDEST-CMV-G93A-SOD1-GFP cDNA (Figure 2A), in combination with Tol2 transposase RNA, resulted in random insertion of the Tol2-flanked transgene into the zebrafish genome. Preliminary studies comparing G93A-SOD1 and G93A-SOD1-GFP RNA injections did not demonstrate an effect of utilizing a GFP-fusion construct on the MN axonal phenotype (data not shown). Germline transmission was confirmed by PCR analysis of genomic DNA from F1 generation embryos for the human transgene (Figure 2B). Western blotting of protein lysates from F1 generation embryos for SOD1 expression (Figure 2C) demonstrated that G93A-SOD1-GFP expression levels were approximately 64% that of the endogenous zebrafish wtSOD1. Transgenic lines expressed the GFP-fusion protein ubiquitously throughout the lifespan of the zebrafish (Figure 2D-E). F2 generation zebrafish from 2 independent lines were characterized for these studies, the G93A-SOD1-GFP.umich1 (G1) and the G93A-SOD1-GFP.umich21 (G21) lines, to minimize the chance of any positional effects of the insertions. All presented data is for the G21 line, as both lines exhibited similar morphological phenotypes.
Symptom onset in transgenic G93A-SOD1 zebrafish
Disease onset in non-transgenic AB control or transgenic G93A-SOD1-GFP zebrafish was determined by quantitative assessment of spontaneous swimming behavior using the Noldus Activity Monitoring System as a clinical correlate to extremity weakness observed in ALS patients (Figure 3). The system integrates movements over a defined interval for various activity parameters, including swimming velocity and time spent swimming versus resting. Examination of spontaneous swim speeds demonstrate that, while the swimming velocity at 10 weeks of age is similar for control and transgenic zebrafish lines, the transgenic G93A-SOD1-GFP zebrafish exhibit an overall slower velocity throughout the disease course compared to AB control zebrafish (Figure 3C). Transgenic G93A-SOD1-GFP zebrafish also tend to spend more time resting as the disease progresses (Figure 3D). Linear regression analysis, on the other hand, reveals that the overall change in swimming velocity between 10-60 weeks of age is approximately zero for both lines; slopes equal 0.0076 and -0.0889 for control AB and transgenic G21 zebrafish, respectively (Figure 3C). This could reflect variable onset among the transgenic G93A-SOD1-GFP zebrafish line that does not reach significance, or may indicate that significant differences in progression might occur later than the 60 week time point. Analysis of the overall change in time resting, however, is significantly different between 10-60 weeks of age for the two lines (Figure 3D). Taken together, these swimming behaviors represent motor dysfunction and decreased endurance that are consistent with a neuromuscular disease, and reflect the eventual onset and progression of disease in transgenic ALS zebrafish.
NMJ and axon pathology in transgenic G93A-SOD1 zebrafish
Effects at the distal axon and NMJ are one of the earliest defects in ALS [3, 4, 24, 25]; therefore, we next investigated neuromuscular pathology in AB control and transgenic G93A-SOD1-GFP zebrafish. NMJ integrity was first examined by quantifying the percentage of colocalized postsynaptic α-bungarotoxin (αBTX)-positive acetylcholine receptor (AChR) clusters and SV2/neurofilament (NF)-positive MN axons (Figure 4A-D; arrowheads depict defects at NMJs). At 10 weeks of age, no differences were observed between control and transgenic G93A-SOD1-GFP zebrafish; however, transgenic G93A-SOD1-GFP zebrafish exhibited a significant loss of intact NMJs after 20 weeks of age that persisted through the 60 week time point (Figure 4E). We next examined how MNs innervated the muscle fibers by qualitatively scoring the innervation patterns at each time point as long, moderately branched, or complex (Figure 5). Control AB zebrafish predominantly exhibited long or moderately branched axons at all examined time points (Figure 4A and Figure 5B), whereas the percentage of complex branched fibers increased around 30 weeks of age in the transgenic G93A-SOD1-GFP zebrafish (Figure 4B and Figure 5B). Together, these data reveal disruption at the NMJ with disease progression in transgenic G93A-SOD1-GFP zebrafish that is followed by alterations in MN innervation patterns that may reflect compensatory remodeling of NMJs.
MN loss in transgenic G93A-SOD1 zebrafish
Axonal and NMJ degeneration is followed by MN loss in ALS patients and transgenic ALS rodents [3, 42], prompting us to next examine MN numbers in the spinal cord of transgenic G93A-SOD1-GFP zebrafish. Spinal cord cross-sections from control AB and transgenic G93A-SOD1-GFP zebrafish were labeled with Cresyl violet Nissl stain and MN loss was evaluated by quantifying the number of large MN cell bodies per ventral horn (Figure 6B-C, arrowheads), an approach validated using transgenic HB9:mGFP zebrafish  which express GFP under control of the MN-specific HB9 promoter (Figure 6A). Control AB zebrafish exhibited a similar number of MNs per ventral horn at every time point examined, whereas transgenic G93A-SOD1-GFP zebrafish exhibited an approximate 50% loss in MN number by 30 weeks of age (Figure 6D). Overall, our findings indicate that G93A-SOD1-GFP expression results in alterations in swimming behavior, NMJ denervation and subsequent effects on NMJ innervation patterns which coincide with MN degeneration and loss.
ALS is a relentlessly progressive neuromuscular disease with no effective treatments. While the cause of MN degeneration in ALS is unknown, multiple lines of evidence support a “dying-back” phenomenon that is characterized by defects at the NMJ and in distal MN axons which is then followed by subsequent MN loss [3, 4, 24, 42]. In vitro studies demonstrate protective effects of caspase inhibition and growth factor therapies on MN survival [21, 41]. However, studies in in vivo models indicate that treatments targeted at MN loss have limited, if any, impact on disease symptoms or survival [20, 43, 44] and that neuroprotective strategies targeted at muscle and NMJs such as growth factor therapies improve symptom onset and severity [24, 41, 45, 46]. Clinical translation of such strategies, however, has not yet been realized and improved treatments for ALS are required. Because defects in distal axons and at NMJs occur before symptom onset and MN loss in ALS, comprehending the mechanisms of ALS neuromuscular pathogenesis is essential for the development of therapies targeted at events prior to the irreversible loss of MNs. Detailed characterization of neuromuscular morphological alterations is possible in zebrafish; therefore, we investigated the effects of G93A-SOD1 expression on zebrafish MNs and NMJs.
In recent years, a handful of studies have demonstrated the consequences of transient expression or knockdown of select ALS mutant proteins in zebrafish embryos [35–39]. Expression of mutant forms of SOD1, for example, induce early defects in MNs including decreased axon length and aberrant branching at 30 hpf, which are attenuated or exacerbated by vascular endothelial growth factor upregulation or knockdown, respectively . Therefore, we first examined the effects of transient human G93A-SOD1 on zebrafish embryo MNs. Because RNA was generated from a pCS2-based construct known to elicit efficient high-level expression in zebrafish, we utilized a dose range that was between 10- to 100-fold lower than the previous report. As seen in Figure 1, our findings confirm the previous axonopathy findings, and further demonstrate that significant effects can be observed even with low levels of G93A-SOD1 RNA. We then examined the therapeutic efficacy of IGF-I upregulation by co-injecting igf-I RNA with G93A-SOD1 RNA and assessing axonal development. Despite the fact that clinical translation of IGF-I therapies for ALS have been slow and not yielded the expected outcomes, IGF-I has been extensively studied for ALS. In vitro and in vivo studies demonstrate that IGF-I activates neuroprotective signaling pathways and provides protection to MNs and at NMJs . Along those lines, IGF-I is capable of preventing the decreased axon length observed with G93A-SOD1 expression in zebrafish embryos (Figure 1C). Together with the previous report , these data demonstrate an effect of low levels of human G93A-SOD1 on zebrafish MNs that is amenable to neuroprotective intervention, providing rationale for the generation of stable transgenic G93A-SOD1 zebrafish lines to examine neuromuscular pathogenesis in ALS.
Zebrafish are easily amenable to genetic manipulation and multiple approaches exist for the generation of stable transgenic disease models. The transgenic zebrafish lines described in this study (Figure 2) were developed using Tol2-mediated transgenesis. Briefly, constructs were generated by multisite Gateway cloning which provides a simple approach to generate Tol2-flanked constructs with various promoters and fusion elements that are amenable to random genomic insertion in the presence of transposase . The CMV promoter we used is derived from the pCS2+ vector used in our transient studies, and a C-terminal GFP-fusion allows for easy detection of germline transmission and confirmation of cellular expression patterns. Confirmation that utilization of a GFP-fusion construct does note exacerbate the phenotype was supported by preliminary studies comparing transient expression of G93A-SOD1 and G93A-SOD1-GFP in embryos (data not shown). Other reports also demonstrate that GFP expression does not affect the mutant SOD1 phenotype in embryos , and the use of GFP in transgenic tissue-specific reporter zebrafish lines like HB9:mGFP zebrafish that express GFP in MNs  provide further evidence supporting our approach. The Tol2-based transposon system supports dramatically increased transgenesis efficiencies , and using this approach, we were able to establish 6 independent transgenic G93A-SOD1-GFP zebrafish lines. The G1 and G21 lines characterized for these studies exhibit ubiquitous expression of the transgene that persists throughout the zebrafish lifespan and through multiple generations.
Transgenic zebrafish are an emerging model for the study of neurodegenerative diseases and at the initiation of this project, long-term data was not available on how chronic G93A-SOD1 expression, or late-onset neurodegeneration in general, would affect zebrafish. Therefore, we first examined swimming behavior as a correlate to muscle weakness observed in ALS patients using the Noldus Larvae Activity Monitoring System. Spontaneous swimming behavior was regularly monitored for quantification of swim speed and duration of movement during a defined interval. Linear regression analysis of AB control and transgenic G93A-SOD1-GFP zebrafish swimming velocity between 10 and 60 weeks of age (Figure 3C) did not reveal a significant difference in overall swim speed change, or slope, between the 2 lines. What is important to note, however, is that examination of the individual time points reveals similar swim speeds at 10 weeks of age, whereas apparent differences in the degree of movement emerge after 20 weeks of age between the 2 lines, with the transgenic G93A-SOD1-GFP zebrafish consistently exhibiting a trend of slightly slower swim speeds. Transgenic G93A-SOD1-GFP zebrafish, however, do tend to spend more time resting at advanced time points compared to controls (Figure 3D). These findings may indicate that, although there are no striking changes in locomotor potential between 10-60 weeks of age in either line, the increased time resting could reflect a higher tendency for fatigue in the transgenic G93A-SOD1-GFP zebrafish that may precede and potentially predict later motor phenotypes. Given the drastic motor deficits that appear in rodent ALS models [22, 49], these modest effects were initially surprising, but during the course of these studies the Beattie laboratory reported the first generation and characterization of a transgenic ALS zebrafish model . In that study, the transgenic zebrafish overexpress zebrafish G93R-sod1 and exhibit decreased swimming endurance at 12 and 16 mo of age compared to non-transgenic controls, as measured by calculating critical swimming speeds against an increasing current flow . These findings suggest that more drastic effects on movement behavior in our model may not be apparent within the 60 week time frame examined and support the continued evaluation of swimming behavior in our transgenic lines, and also suggest the possibility that muscle strength defects in our model may become apparent by exploring additional assays for motor function.
As previously described, neuromuscular defects are apparent in ALS rodent models and patients well before symptom onset and MN loss [3, 4]; therefore, we next performed a detailed pathological characterization of NMJs and MNs in control and transgenic G93A-SOD1-GFP zebrafish. Techniques to quantify NMJ innervation by MNs are well-established in rodents [25, 45, 50, 51] and larval zebrafish [52–55], however, only limited information regarding visualization and quantification of juvenile and adult zebrafish NMJ integrity are available [32, 56]. For the current studies, we labeled presynaptic MNs and postsynaptic AChR clusters with SV2/NF and αBTX, respectively, and quantified the number of intact NMJs in control AB and transgenic G93A-SOD1-GFP zebrafish between 10 and 60 weeks of age (Figure 4). Transgenic G93A-SOD1-GFP zebrafish exhibited a significant loss of intact NMJs at 20 weeks of age that was consistent across the remaining time points. These effects at the NMJ even in the absence of significant symptomatic affects are consistent with those observed in rodent ALS models. Transgenic G93A-SOD1 mice exhibit a 40% reduction in the number of innervated NMJs at 47 days of age, while symptomatic onset does not occur until around 80 days of age . The importance of NMJ maintenance is further supported by a study where muscle-specific expression of mutant SOD1 in mice promoted NMJ defects and distal axonal degeneration . Interestingly, we noticed that this early decrease in NMJ integrity at 20 weeks of age was subsequently followed by alterations in the innervation patterns of the muscle that became apparent at the 30 week time point. While imaging at earlier time points revealed long axons that synapse along longitudinal sections of myofibers, later time points exhibited more branched nerve fibers that synapsed across multiple myofibers (Figure 5). These changes are significant in transgenic G93A-SOD1-GFP zebrafish beginning at 30 weeks of age, and may reflect compensatory reinnervation of myofibers as previously observed in transgenic ALS mice [24, 42]. MN loss follows NMJ denervation and distal axonal degeneration in patients and rodent ALS models [3, 42]; therefore, we also quantified MN numbers in transgenic G93A-SOD1-GFP zebrafish between 30-60 weeks of age. We observed an approximate 50% reduction in the number of large MN cell bodies in the ventral horn of transgenic G93A-SOD1-GFP zebrafish spinal cords at 40 weeks of age compared to age-matched controls (Figure 6). This coincides with the sequence of events in ALS rodent models, as MN loss is evident around 100 days in G93A-SOD1 mice, relative to initial NMJ defects as early as 47 days, and death at 131 days . A timeline summarizing the sequence of observed morphological alterations in our transgenic G93A-SOD1-GFP zebrafish is depicted in Figure 7.
This characterization of our novel transgenic G93A-SOD1-GFP zebrafish supports and builds upon the previous findings in transgenic G93R-sod1 zebrafish . The transgenic G93R-sod1 zebrafish exhibit a small but significant reduction in NMJ innervation at 11 days post-fertilization (dpf) in transgenic larvae, and a more significant loss of NMJ innervation at 12 months of age . While intermediate time points were not described, these findings coincide with our later observations of NMJ loss between 40-60 weeks of age, and reflect the fact that significant NMJ defects are evident as early as 20 weeks of age in our model. MN loss is also evident in both models, with our transgenic G93A-SOD1 zebrafish exhibiting an approximate 50% reduction in MN numbers by 40 weeks of age, and the transgenic G93R-sod1 zebrafish having a 38% reduction in MN numbers at end-stage disease. In addition, transgenic G93R-sod1 zebrafish lines exhibit reductions in survival that become evident around 18-22 months of age . While preliminary findings in our transgenic G93A-SOD1-GFP zebrafish did not reveal any noticeable differences in survival (data not shown), it is possible based on the findings in the other model that the 60 week time frame for the current study was too short to detect quantifiable differences, and studies are currently ongoing to determine when or if survival is affected in our model. Taken together, both models depict a sequence of events that supports the “dying-back” theory in ALS, where defects at the NMJ are the first insult and then lead to progressive distal axonal degeneration and subsequent MN loss [3, 4, 24, 42].
This newly established ALS model provides an additional system that will supplement our understanding of ALS neuromuscular pathology in the context of disease progression. While we observe significant neuromuscular defects that reflect a characteristic distal-to-proximal ALS disease course, there are still unanswered questions that should be addressed to understand why the disease course is not as severe in zebrafish as it is in G93A-SOD1 rodents, and why drastic locomotor deficits are not observed even in the context of MN loss. A possible explanation for the reduced disease severity could relate to the relative expression levels of mutant SOD1 in the transgenic models. Our data indicate that G93A-SOD1-GFP expression levels were approximately 64% that of the endogenous zebrafish wtSOD1 (Figure 2C), whereas transgenic G93A-SOD1 rodent models exhibit robust levels of overexpression . It is also possible that the disease course is affected by the regenerative ability of zebrafish; while neuronal regeneration is not prevalent in human or rodent spinal cords, evidence of MN regeneration has been reported in zebrafish . The observed alterations in NMJ innervation patterns (Figure 4) support the possibility of compensatory reinnervation, and although we observe a decrease in MN numbers in transgenic G93A-SOD1-GFP zebrafish, regenerative markers must be examined in spinal cords to understand what role regeneration plays in disease pathogenesis in this model. Non-cell autonomous effects including astrocytes and glial activation also have a significant impact on disease progression in rodent ALS models [1, 2]; therefore, the contributions of these cell types to disease pathogenesis should also be investigated. The impact of G93A-SOD1-GFP expression on other neuronal populations is also of interest, although no effects were seen on the commissural axon of the Mauthner neuron in the hindbrain, on sensory neurons of the lateral line, or on Rohon-Beard sensory neurons in the transient model described by Lemmens et al . Finally, a detailed analysis of MN axons using electron microscopy and IHC with advanced imaging techniques to comprehend the mechanisms of axonal degeneration throughout the disease course will provide important insight into the “dying-back” mechanisms of MN degeneration in ALS [3, 4, 24, 42].
In summary, the novel transgenic G93A-SOD1-GFP zebrafish exhibit defects at the NMJ around 20 weeks of age, subsequent alterations in innervation patterns at 30 weeks of age, and evidence of MN loss around 40 weeks of age (Figure 7). These neuromuscular findings support the utilization of zebrafish as a model organism to investigate early events in ALS disease pathogenesis for therapeutic development. Furthermore, the generation of transgenic ALS zebrafish models expressing other familial ALS mutations such as TDP43 should be considered, as they have the potential to provide additional insight into the mechanisms of ALS onset and progression.
All zebrafish (Danio rerio) were maintained and handled in compliance with national and local animal welfare bodies and utilized per approved protocols (University of Michigan's Institutional Animal Care and Use Committee protocol #09835). Embryos were collected after timed mating of adult AB strain zebrafish for microinjection to establish the transient ALS models or generate the transgenic ALS lines used in these studies. Transgenic HB9:mGFP zebrafish  which express GFP in MNs were used to confirm the MN identification approach utilized for MN quantification. The transgenic ALS zebrafish described in this manuscript are available by contacting email@example.com, or if there is sufficient interest we will provide the lines through the Zebrafish International Resource Center (ZIRC; http://zebrafish.org/zirc/home).
Transient overexpression of G93A-SOD1 in zebrafish
RNA generated from pCS2+ vectors allows for efficient transient expression of proteins in zebrafish embryos. To generate transient ALS zebrafish models, full length clones to human wtSOD1 and G93A-SOD1 were subcloned into pCS2+ followed by restriction digest to linearize the constructs for generation of RNA using the mMessage mMachine SP6 kit (Ambion). The pCS2-based zebrafish igf-I construct was provided by Dr. Cunming Duan for igf-I RNA generation . mRNA was microinjected into the yolk sac of 1-4 cell embryos and embryos were incubated at 28oC in 0.5X E2 media until processing for IHC or western blotting as previously described [60, 61]. For IHC, microinjected zebrafish embryos and UIC zebrafish embryos were deyolked at 30 hpf and fixed overnight in 4% PFA. Fixed embryos were washed with 1x PBS and blocked for 2 h at room temperature in 2% BSA, 0.5% Triton-X 100 and 0.05% DMSO before incubation with the SV2 primary antibody (1:200; Developmental Studies Hybridoma Bank) overnight at 4oC. Embryos were then washed and incubated with AlexaFluor-594-conjugated anti-mouse secondary antibody (1:250, Invitrogen) for 2 h at room temperature, washed, and mounted onto glass slides using ProLong-Gold antifade reagent (Invitrogen, Eugene, OR). Slides were blinded and imaged using a Nikon AZ100 microscope and NIS Elements BS software. The length from the tip of the motor axon to the point where it exits the spinal cord was measured for 5 axons per embryo, corresponding to axons 13-17 using NIS Elements BS software. A minimum of 30 embryos per condition over 3 independent experiments were assessed for each data set.
Western blotting using standard protocols [21, 45] was utilized to confirm SOD1 expression and activation of downstream IGF-I pathways. Briefly, microinjected zebrafish embryos and UIC zebrafish embryos were dechorionated at 30 hpf and protein lysates were prepared in RIPA buffer (20 mM Tris, pH 7.4, 150 mM NaCl, 1 mM EDTA, 0.1% SDS, 1 mM Na deocycholate, 1% Triton X-100, 0.1 trypsin units/L aprotinin, 10 mg/ml leupeptin, and 50 mg/ml PMSF; 15 μl per embryo). Equal amounts of protein were loaded into each lane of a 12.5% or 15% polyacrylamide gel for analysis of phospho-Akt/phospho-MAPK or SOD1 expression, respectively, and transferred to nitrocellulose. Blots were incubated with primary antibodies overnight at 4oC followed by incubation with appropriate horseradish peroxidase-conjugated secondary antibodies (Santa Cruz Biotechnology, Santa Cruz, CA). The following antibodies were utilized: phospho-Akt (1:1000; Cell Signaling, Danvers, MA), phospho-p44/42 MAPK (Thr202/tyr204; 1:1000; Cell Signaling), SOD-100 (1:5000; Stressgen, Victoria, Canada), and GAPDH (1:5000; Chemicon, Temecula, CA). Results following enhanced chemiluminescence with LumiGlO plus Peroxide reagent (Cell Signaling) were detected using the BioRad ChemiDoc XRS imaging system with Quantity One analysis software. GAPDH was used to confirm equal protein loading.
Generation of transgenic ALS zebrafish lines
Stable transgenic zebrafish expressing G93A-SOD1-GFP were generated using Tol2-mediated transgenesis . Constructs were generated with the Gateway-based cloning system using Tol2-constructs kindly provided by Dr. Chi-Bin Chien. Final constructs utilized the pDEST destination vector including a CMV promoter element, G93A-SOD1 and a 3’ EGFP-fusion element with an SV40 late polyadenylation signal . cDNA (10 ng/μl) was microinjected into 1-4 cell zebrafish embryos as described above along with Tol2 transposase RNA (100 ng/μl) to facilitate Tol2-mediated random integration into the genome. P0 transgenic embryos were raised to maturity and screened for germline incorporation. Successful generation of transgenic lines was evident via GFP expression in F1 generation embryos, or alternatively, via PCR analysis of genomic DNA from F1 generation embryos. Briefly, genomic DNA was extracted from 24 hpf embryos following standard protocols  and PCR-screened for the presence of incorporated human SOD1 cDNA using the following primers: forward 5’-TTCGAGCAGAAGGAAAGTAATGGA-3’, reverse 5’-ACATTGCCCAAGTCTCCAACATGC-3’. Characterization of established transgenic lines was performed on F2 generation zebrafish and compared to age-matched control AB strain zebrafish. Stable transgene expression throughout the zebrafish lifespan and across generations was confirmed by assessing GFP fluorescence at each time point. Furthermore, western blotting was utilized to assay G93A-SOD1-GFP protein expression levels in transgenic 24-48 hpf embryos according to the protocol detailed above. Results are representative of at least 3 independent experiments from 3 clutches and densitometry results were normalized to GAPDH to quantify protein expression levels using Quantity One analysis software.
Zebrafish activity monitoring
Swimming behavior was assessed using the rapid detection Noldus Larvae Activity Monitoring System (http://www.noldus.com/site/dpc200711027). An infrared camera tracks fish movements over a defined time interval and movements are then converted into distance tracks for analysis according to multiple activity parameters using the system’s proprietary software package, EthoVision XT 6.0. The system accommodates various sized multi-well plates to allow for quick and easy quantification of movement throughout the lifespan of the transgenic zebrafish. For activity monitoring, AB (n = 7) and transgenic G93A-SOD1-GFP (n = 11) zebrafish were monitored weekly between the ages of 10 and 30 weeks and every other week between 30 and 70 weeks of age, for 10 minutes to record spontaneous swimming behavior. Results were analyzed for average swimming velocity and time resting during the 10 minute monitoring interval.
MN axonal and NMJ defects and degeneration were examined by IHC per standard protocols [45, 52, 55]. Briefly, adult AB and transgenic G93A-SOD1-GFP zebrafish were euthanized at 10, 20, 30, 40, and 60 weeks of age. Fish were fixed overnight in 2% PFA, cryoprotected in PBS containing 30% sucrose and muscle tissue was dissected and embedded in OCT for sectioning and IHC. Longitudinal sections (20 μm) were obtained on a Leica cryostat and then processed for IHC using our previously published method [60, 63]. Briefly, sections were blocked for 30 minutes with 5% normal goat serum / 0.1% triton-X100 / 1x PBS and incubated with AlexaFluor 647-conjugated αBTX (1:1000; Molecular Probes, Invitrogen, Carlsbad) for 30 minutes to label postsynaptic AChRs. Sections were then washed and incubated overnight with SV2 (1:200; Hybridoma Bank, Iowa City, IA) and NF (1:1000; Chemicon) primary antibodies to label MN axons. After washing, Alexa Fluor 594-conjugated IgG secondary antibody (Molecular Probes) was applied for 2 h, followed by rinsing and mounting in Prolong Gold antifade reagent with DAPI (Invitrogen). Slides were blinded for z-stack imaging with a Zeiss Apotome or a Nikon A1 laser scanning confocal system with TiE microscope. Imaging was done at the University of Michigan Microscopy & Image Analysis Core (http://www.med.umich.edu/mil/index.htm) or the Morphology and Image Analysis Core of the Michigan Diabetes Research and Training Center (http://www.med.umich.edu/mdrtc/cores/MIACCore/index.html), respectively.
Differences in NMJ integrity were determined by examining SV2/NF and αBTX co-localization to calculate the percent of innervated junctions and by examining NMJ innervation patterns. The percent of innervated synapses was determined by calculating the number of AChR clusters fully or partially innervated by nerve terminals as previously described using MetaMorph (version 7.4.4, Molecular Devices, Sunnyvale, CA) [40, 45, 50, 64, 65]. At least 3 random images per slide were acquired from 3 zebrafish per time point, for a minimum of 9 images per line. Elements software was used to visualize orthogonal XZ and YZ views of the z-seried to confirm colocalization. Qualitative assessment of innervation patterns in the muscle consisted of assessing branching patterns of individual fibers for at least 3 images per slide from 3 zebrafish per time point per condition. Innervation patterns were scored as long, moderately branched or complex. Long axons (score of 1) are axons that do not exhibit a major branch point along the length of visible axonal segment. Moderately branched axons are nerve fibers that exhibit a single major branch point (score of 2) or two branch points (score of 3). Complex innervation patterns consist of segments with three or more major branches (score of 4+).
Motor neuron quantification
MN loss was determined by counting the total number of MNs in spinal cord sections . Adult AB and transgenic ALS zebrafish at 30, 40, and 60 weeks of age were fixed and cryoprotected and spinal cords were dissected and prepared for sectioning as described above for muscle tissue. MNs in 20 μm cross-sections were labeled with Cresyl violet Nissl stain (Catalog # C-3886; Sigma, St. Louis, MO) as previously described . Briefly, sections were stained with Cresyl violet and cleared and dehydrated prior to mounting with DPX. Images were acquired using a Nikon Microphot-FXA microscope and the number of spinal MNs per spinal cord ventral horn was quantified for multiple sections throughout the spinal cord from at least 3 fish per line at each time point. The approach identifying MNs by cell body size and localization to the ventral horn was validated using transgenic HB9:mGFP zebrafish.
All results are representative of at least 3 independent experiments and slides were blinded prior to imaging and analysis. Multiple independent transgenic ALS zebrafish lines were generated to minimize any positional effects of the transgene insertion. Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparison test or linear regression analysis (GraphPad Prism).
Amyotrophic lateral sclerosis
Familial amyotrophic lateral sclerosis
Fused in sarcoma
Insulin-like growth factor-I
Mitogen activated protein kinase
Cu2+/Zn2+ superoxide dismutase
TAR DNA binding protein 43
Wild-type Cu2+/Zn2+ superoxide dismutase
Zebrafish international resource center.
Boillee S, Vande Velde C, Cleveland DW: ALS: a disease of motor neurons and their nonneuronal neighbors. Neuron. 2006, 52: 39-59. 10.1016/j.neuron.2006.09.018.
Ilieva H, Polymenidou M, Cleveland DW: Non-cell autonomous toxicity in neurodegenerative disorders: ALS and beyond. J Cell Biol. 2009, 187: 761-772. 10.1083/jcb.200908164.
Fischer LR, Culver DG, Tennant P, Davis AA, Wang M, Castellano-Sanchez A, Khan J, Polak MA, Glass JD: Amyotrophic lateral sclerosis is a distal axonopathy: evidence in mice and man. Exp Neurol. 2004, 185: 232-240. 10.1016/j.expneurol.2003.10.004.
Fischer LR, Glass JD: Axonal degeneration in motor neuron disease. Neurodegener Dis. 2007, 4: 431-442. 10.1159/000107704.
Chen YZ, Bennett CL, Huynh HM, Blair IP, Puls I, Irobi J, Dierick I, Abel A, Kennerson ML, Rabin BA, et al: DNA/RNA helicase gene mutations in a form of juvenile amyotrophic lateral sclerosis (ALS4). Am J Hum Genet. 2004, 74: 1128-1135. 10.1086/421054.
Greenway MJ, Andersen PM, Russ C, Ennis S, Cashman S, Donaghy C, Patterson V, Swingler R, Kieran D, Prehn J, et al: ANG mutations segregate with familial and 'sporadic' amyotrophic lateral sclerosis. Nat Genet. 2006, 38: 411-413. 10.1038/ng1742.
Hadano S, Hand CK, Osuga H, Yanagisawa Y, Otomo A, Devon RS, Miyamoto N, Showguchi-Miyata J, Okada Y, Singaraja R, et al: A gene encoding a putative GTPase regulator is mutated in familial amyotrophic lateral sclerosis 2. Nat Genet. 2001, 29: 166-173. 10.1038/ng1001-166.
Kwiatkowski TJ, Bosco DA, Leclerc AL, Tamrazian E, Vanderburg CR, Russ C, Davis A, Gilchrist J, Kasarskis EJ, Munsat T, et al: Mutations in the FUS/TLS gene on chromosome 16 cause familial amyotrophic lateral sclerosis. Science. 2009, 323: 1205-1208. 10.1126/science.1166066.
Lagier-Tourenne C, Cleveland DW: Rethinking ALS: the FUS about TDP-43. Cell. 2009, 136: 1001-1004. 10.1016/j.cell.2009.03.006.
Munch C, Sedlmeier R, Meyer T, Homberg V, Sperfeld AD, Kurt A, Prudlo J, Peraus G, Hanemann CO, Stumm G, Ludolph AC: Point mutations of the p150 subunit of dynactin (DCTN1) gene in ALS. Neurology. 2004, 63: 724-726. 10.1212/01.WNL.0000134608.83927.B1.
Nishimura AL, Mitne-Neto M, Silva HC, Richieri-Costa A, Middleton S, Cascio D, Kok F, Oliveira JR, Gillingwater T, Webb J, et al: A mutation in the vesicle-trafficking protein VAPB causes late-onset spinal muscular atrophy and amyotrophic lateral sclerosis. Am J Hum Genet. 2004, 75: 822-831. 10.1086/425287.
Puls I, Jonnakuty C, LaMonte BH, Holzbaur EL, Tokito M, Mann E, Floeter MK, Bidus K, Drayna D, Oh SJ, et al: Mutant dynactin in motor neuron disease. Nat Genet. 2003, 33: 455-456. 10.1038/ng1123.
Rosen DR, Siddique T, Patterson D, Figlewicz DA, Sapp P, Hentati A, Donaldson D, Goto J, O'Regan JP, Deng HX, et al: Mutations in Cu/Zn superoxide dismutase are associated with familial amyotrophic lateral sclerosis. Nature. 1993, 362: 59-62. 10.1038/362059a0.
Yang Y, Hentati A, Deng HX, Dabbagh O, Sasaki T, Hirano M, Hung WY, Ouahchi K, Yan J, Azim AC, et al: The gene encoding alsin, a protein with three guanine-nucleotide exchange factor domains, is mutated in a form of recessive amyotrophic lateral sclerosis. Nat Genet. 2001, 29: 160-165. 10.1038/ng1001-160.
Deng HX, Chen W, Hong ST, Boycott KM, Gorrie GH, Siddique N, Yang Y, Fecto F, Shi Y, Zhai H, et al: Mutations in UBQLN2 cause dominant X-linked juvenile and adult-onset ALS and ALS/dementia. Nature. 2011, 477: 211-215. 10.1038/nature10353.
Maruyama H, Morino H, Ito H, Izumi Y, Kato H, Watanabe Y, Kinoshita Y, Kamada M, Nodera H, Suzuki H, et al: Mutations of optineurin in amyotrophic lateral sclerosis. Nature. 2010, 465: 223-226. 10.1038/nature08971.
DeJesus-Hernandez M, Mackenzie IR, Boeve BF, Boxer AL, Baker M, Rutherford NJ, Nicholson AM, Finch NA, Flynn H, Adamson J, et al: Expanded GGGGCC hexanucleotide repeat in noncoding region of C9ORF72 causes chromosome 9p-linked FTD and ALS. Neuron. 2011, 72: 245-256. 10.1016/j.neuron.2011.09.011.
Renton AE, Majounie E, Waite A, Simon-Sanchez J, Rollinson S, Gibbs JR, Schymick JC, Laaksovirta H, van Swieten JC, Myllykangas L, et al: A hexanucleotide repeat expansion in C9ORF72 is the cause of chromosome 9p21-linked ALS-FTD. Neuron. 2011, 72: 257-268. 10.1016/j.neuron.2011.09.010.
Joyce PI, Fratta P, Fisher EM, Acevedo-Arozena A: SOD1 and TDP-43 animal models of amyotrophic lateral sclerosis: recent advances in understanding disease toward the development of clinical treatments. Mamm Genome. 2011, 22: 420-448. 10.1007/s00335-011-9339-1.
Li M, Ona VO, Guegan C, Chen M, Jackson-Lewis V, Andrews LJ, Olszewski AJ, Stieg PE, Lee JP, Przedborski S, Friedlander RM: Functional role of caspase-1 and caspase-3 in an ALS transgenic mouse model. Science. 2000, 288: 335-339. 10.1126/science.288.5464.335.
Lunn JS, Sakowski SA, Kim B, Rosenberg AA, Feldman EL: Vascular endothelial growth factor prevents G93A-SOD1-induced motor neuron degeneration. Dev Neurobiol. 2009, 69: 871-884. 10.1002/dneu.20747.
Gurney ME, Pu H, Chiu AY, Dal Canto MC, Polchow CY, Alexander DD, Caliendo J, Hentati A, Kwon YW, Deng HX: Motor neuron degeneration in mice that express a human Cu,Zn superoxide dismutase mutation. Science. 1994, 264: 1772-1775. 10.1126/science.8209258.
Peviani M, Caron I, Pizzasegola C, Gensano F, Tortarolo M, Bendotti C: Unraveling the complexity of amyotrophic lateral sclerosis: recent advances from the transgenic mutant SOD1 mice. CNS Neurol Disord Drug Targets. 2010, 9: 491-503.
Dadon-Nachum M, Melamed E, Offen D: The "dying-back" phenomenon of motor neurons in ALS. J Mol Neurosci. 2011, 43: 470-477. 10.1007/s12031-010-9467-1.
Dupuis L, Loeffler JP: Neuromuscular junction destruction during amyotrophic lateral sclerosis: insights from transgenic models. Curr Opin Pharmacol. 2009, 9: 341-346. 10.1016/j.coph.2009.03.007.
Kabashi E, Brustein E, Champagne N, Drapeau P: Zebrafish models for the functional genomics of neurogenetic disorders. Biochim Biophys Acta. 2011, 1812: 335-345. 10.1016/j.bbadis.2010.09.011.
Kabashi E, Champagne N, Brustein E, Drapeau P: In the swim of things: recent insights to neurogenetic disorders from zebrafish. Trends Genet. 2010, 26: 373-381. 10.1016/j.tig.2010.05.004.
Flanagan-Steet H, Fox MA, Meyer D, Sanes JR: Neuromuscular synapses can form in vivo by incorporation of initially aneural postsynaptic specializations. Development. 2005, 132: 4471-4481. 10.1242/dev.02044.
Higashijima S, Okamoto H, Ueno N, Hotta Y, Eguchi G: High-frequency generation of transgenic zebrafish which reliably express GFP in whole muscles or the whole body by using promoters of zebrafish origin. Dev Biol. 1997, 192: 289-299. 10.1006/dbio.1997.8779.
Kim MJ, Kang KH, Kim CH, Choi SY: Real-time imaging of mitochondria in transgenic zebrafish expressing mitochondrially targeted GFP. Biotechniques. 2008, 45: 331-334. 10.2144/000112909.
Meng A, Tang H, Ong BA, Farrell MJ, Lin S: Promoter analysis in living zebrafish embryos identifies a cis-acting motif required for neuronal expression of GATA-2. Proc Natl Acad Sci USA. 1997, 94: 6267-6272. 10.1073/pnas.94.12.6267.
Boon KL, Xiao S, McWhorter ML, Donn T, Wolf-Saxon E, Bohnsack MT, Moens CB, Beattie CE: Zebrafish survival motor neuron mutants exhibit presynaptic neuromuscular junction defects. Hum Mol Genet. 2009, 18: 3615-3625. 10.1093/hmg/ddp310.
le Hao T, Burghes AH, Beattie CE: Generation and Characterization of a genetic zebrafish model of SMA carrying the human SMN2 gene. Mol Neurodegener. 2011, 6: 24-10.1186/1750-1326-6-24.
McWhorter ML, Monani UR, Burghes AH, Beattie CE: Knockdown of the survival motor neuron (Smn) protein in zebrafish causes defects in motor axon outgrowth and pathfinding. J Cell Biol. 2003, 162: 919-931. 10.1083/jcb.200303168.
Kabashi E, Bercier V, Lissouba A, Liao M, Brustein E, Rouleau GA, Drapeau P: FUS and TARDBP but not SOD1 interact in genetic models of amyotrophic lateral sclerosis. PLoS Genet. 2011, 7: e1002214-10.1371/journal.pgen.1002214.
Lemmens R, Van Hoecke A, Hersmus N, Geelen V, D'Hollander I, Thijs V, Van Den Bosch L, Carmeliet P, Robberecht W: Overexpression of mutant superoxide dismutase 1 causes a motor axonopathy in the zebrafish. Hum Mol Genet. 2007, 16: 2359-2365. 10.1093/hmg/ddm193.
Gros-Louis F, Kriz J, Kabashi E, McDearmid J, Millecamps S, Urushitani M, Lin L, Dion P, Zhu Q, Drapeau P, et al: Als2 mRNA splicing variants detected in KO mice rescue severe motor dysfunction phenotype in Als2 knock-down zebrafish. Hum Mol Genet. 2008, 17: 2691-2702. 10.1093/hmg/ddn171.
Simpson CL, Lemmens R, Miskiewicz K, Broom WJ, Hansen VK, van Vught PW, Landers JE, Sapp P, Van Den Bosch L, Knight J, et al: Variants of the elongator protein 3 (ELP3) gene are associated with motor neuron degeneration. Hum Mol Genet. 2009, 18: 472-481.
Kabashi E, Lin L, Tradewell ML, Dion PA, Bercier V, Bourgouin P, Rochefort D, Bel Hadj S, Durham HD, Vande Velde C, et al: Gain and loss of function of ALS-related mutations of TARDBP (TDP-43) cause motor deficits in vivo. Hum Mol Genet. 2010, 19: 671-683. 10.1093/hmg/ddp534.
Ramesh T, Lyon AN, Pineda RH, Wang C, Janssen PM, Canan BD, Burghes AH, Beattie CE: A genetic model of amyotrophic lateral sclerosis in zebrafish displays phenotypic hallmarks of motoneuron disease. Dis Model Mech. 2010, 3: 652-662. 10.1242/dmm.005538.
Sakowski SA, Schuyler AD, Feldman EL: Insulin-like growth factor-I for the treatment of amyotrophic lateral sclerosis. Amyotroph Lateral Scler. 2009, 10: 63-73. 10.1080/17482960802160370.
Frey D, Schneider C, Xu L, Borg J, Spooren W, Caroni P: Early and selective loss of neuromuscular synapse subtypes with low sprouting competence in motoneuron diseases. J Neurosci. 2000, 20: 2534-2542.
Gould TW, Buss RR, Vinsant S, Prevette D, Sun W, Knudson CM, Milligan CE, Oppenheim RW: Complete dissociation of motor neuron death from motor dysfunction by Bax deletion in a mouse model of ALS. J Neurosci. 2006, 26: 8774-8786. 10.1523/JNEUROSCI.2315-06.2006.
Sagot Y, Dubois-Dauphin M, Tan SA, de Bilbao F, Aebischer P, Martinou JC, Kato AC: Bcl-2 overexpression prevents motoneuron cell body loss but not axonal degeneration in a mouse model of a neurodegenerative disease. J Neurosci. 1995, 15: 7727-7733.
Sakowski SA, Heavener SB, Lunn JS, Fung K, Oh SS, Spratt SK, Hogikyan ND, Feldman EL: Neuroprotection using gene therapy to induce vascular endothelial growth factor-A expression. Gene Ther. 2009, 16: 1292-1299. 10.1038/gt.2009.111.
Dupuis L, Echaniz-Laguna A: Skeletal muscle in motor neuron diseases: therapeutic target and delivery route for potential treatments. Curr Drug Targets. 2010, 11: 1250-1261. 10.2174/1389450111007011250.
Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, Parant JM, Yost HJ, Kanki JP, Chien CB: The Tol2kit: a multisite gateway-based construction kit for Tol2 transposon transgenesis constructs. Dev Dyn. 2007, 236: 3088-3099. 10.1002/dvdy.21343.
Kawakami K, Takeda H, Kawakami N, Kobayashi M, Matsuda N, Mishina M: A transposon-mediated gene trap approach identifies developmentally regulated genes in zebrafish. Dev Cell. 2004, 7: 133-144. 10.1016/j.devcel.2004.06.005.
Mancuso R, Olivan S, Osta R, Navarro X: Evolution of gait abnormalities in SOD1(G93A) transgenic mice. Brain Res. 2011, 1406: 65-73.
Rubin A, Mobley B, Hogikyan N, Bell K, Sullivan K, Boulis N, Feldman E: Delivery of an adenoviral vector to the crushed recurrent laryngeal nerve. Laryngoscope. 2003, 113: 985-989. 10.1097/00005537-200306000-00013.
Jang YC, Lustgarten MS, Liu Y, Muller FL, Bhattacharya A, Liang H, Salmon AB, Brooks SV, Larkin L, Hayworth CR, et al: Increased superoxide in vivo accelerates age-associated muscle atrophy through mitochondrial dysfunction and neuromuscular junction degeneration. FASEB J. 2010, 24: 1376-1390. 10.1096/fj.09-146308.
Hirata H, Watanabe T, Hatakeyama J, Sprague SM, Saint-Amant L, Nagashima A, Cui WW, Zhou W, Kuwada JY: Zebrafish relatively relaxed mutants have a ryanodine receptor defect, show slow swimming and provide a model of multi-minicore disease. Development. 2007, 134: 2771-2781. 10.1242/dev.004531.
Lefebvre JL, Jing L, Becaficco S, Franzini-Armstrong C, Granato M: Differential requirement for MuSK and dystroglycan in generating patterns of neuromuscular innervation. Proc Natl Acad Sci USA. 2007, 104: 2483-2488. 10.1073/pnas.0610822104.
Panzer JA, Song Y, Balice-Gordon RJ: In vivo imaging of preferential motor axon outgrowth to and synaptogenesis at prepatterned acetylcholine receptor clusters in embryonic zebrafish skeletal muscle. J Neurosci. 2006, 26: 934-947. 10.1523/JNEUROSCI.3656-05.2006.
Saint-Amant L, Sprague SM, Hirata H, Li Q, Cui WW, Zhou W, Poudou O, Hume RI, Kuwada JY: The zebrafish ennui behavioral mutation disrupts acetylcholine receptor localization and motor axon stability. Dev Neurobiol. 2008, 68: 45-61. 10.1002/dneu.20569.
Panzer JA, Gibbs SM, Dosch R, Wagner D, Mullins MC, Granato M, Balice-Gordon RJ: Neuromuscular synaptogenesis in wild-type and mutant zebrafish. Dev Biol. 2005, 285: 340-357. 10.1016/j.ydbio.2005.06.027.
Wong M, Martin LJ: Skeletal muscle-restricted expression of human SOD1 causes motor neuron degeneration in transgenic mice. Hum Mol Genet. 2010, 19: 2284-2302. 10.1093/hmg/ddq106.
Reimer MM, Sorensen I, Kuscha V, Frank RE, Liu C, Becker CG, Becker T: Motor neuron regeneration in adult zebrafish. J Neurosci. 2008, 28: 8510-8516. 10.1523/JNEUROSCI.1189-08.2008.
Maures T, Chan SJ, Xu B, Sun H, Ding J, Duan C: Structural, biochemical, and expression analysis of two distinct insulin-like growth factor I receptors and their ligands in zebrafish. Endocrinology. 2002, 143: 1858-1871. 10.1210/en.143.5.1858.
Dowling JJ, Gibbs E, Russell M, Goldman D, Minarcik J, Golden JA, Feldman EL: Kindlin-2 is an essential component of intercalated discs and is required for vertebrate cardiac structure and function. Circ Res. 2008, 102: 423-431. 10.1161/CIRCRESAHA.107.161489.
Dowling JJ, Vreede AP, Low SE, Gibbs EM, Kuwada JY, Bonnemann CG, Feldman EL: Loss of myotubularin function results in T-tubule disorganization in zebrafish and human myotubular myopathy. PLoS Genet. 2009, 5: e1000372-10.1371/journal.pgen.1000372.
Westerfield M: The Zebrafish Book: A Guide for the Laboratory Use of Zebrafish (Danio rerio). 2007, Eugene, OR: The University of Oregon Press, 5
Dowling JJ, Gibbs EM, Feldman EL: Membrane traffic and muscle: lessons from human disease. Traffic. 2008, 9: 1035-1043. 10.1111/j.1600-0854.2008.00716.x.
Rouaux C, Panteleeva I, Rene F, de Aguilar JL G, Echaniz-Laguna A, Dupuis L, Menger Y, Boutillier AL, Loeffler JP: Sodium valproate exerts neuroprotective effects in vivo through CREB-binding protein-dependent mechanisms but does not improve survival in an amyotrophic lateral sclerosis mouse model. J Neurosci. 2007, 27: 5535-5545. 10.1523/JNEUROSCI.1139-07.2007.
Rubin AD, Hogikyan ND, Sullivan K, Boulis N, Feldman EL: Remote delivery of rAAV-GFP to the rat brainstem through the recurrent laryngeal nerve. Laryngoscope. 2001, 111: 2041-2045. 10.1097/00005537-200111000-00032.
Kim H, Kim HY, Choi MR, Hwang S, Nam KH, Kim HC, Han JS, Kim KS, Yoon HS, Kim SH: Dose-dependent efficacy of ALS-human mesenchymal stem cells transplantation into cisterna magna in SOD1-G93A ALS mice. Neurosci Lett. 2010, 468: 190-194. 10.1016/j.neulet.2009.10.074.
Boulis NM, Turner DE, Imperiale MJ, Feldman EL: Neuronal survival following remote adenovirus gene delivery. J Neurosurg. 2002, 96: 212-219.
The authors would like to thank Dr. Cunming Duan for kindly providing the pCS2-igf-I construct, Dr. Chi-Bin Chien for kindly providing the Tol2kit constructs, and Dr. John Kuwada for kindly providing the transgenic HB9:mGFP zebrafish. The authors also appreciate the services of the University of Michigan Microscopy & Image Analysis Laboratory Core facility (http://www.med.umich.edu/mil/index.htm) and the Morphology and Image Analysis Core of the Michigan Diabetes Research and Training Center (http://www.med.umich.edu/mdrtc/cores/MIACCore/index.html) which is supported by Grant #P60DK020572 from the NIDDK. The authors would like to thank Mrs. Judith Bentley for excellent secretarial support during the preparation of this manuscript. This work is supported by the A. Alfred Taubman Medical Research Institute; the Program for Neurology Research & Discovery; and the National Institutes of Health [T32 NS007222-28 to S.A.S.].
The authors have no competing or financial interests to declare.
SAS initiated and designed the study, made the transgenic lines, and designed and performed the experiments described in this manuscript. ASB assisted with all experiments and managed the zebrafish facility. GZ-B, MP, AAR, and SGP were involved in the transient ALS model experiments. SSO contributed to the transgenic zebrafish experiments. Data analysis was performed by SAS and JSL. SAS wrote the manuscript. JSL, JJD, and ELF provided critical feedback on experimental design and edited the manuscript. All authors read and approved the final manuscript.
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Sakowski, S.A., Lunn, J.S., Busta, A.S. et al. Neuromuscular effects of G93A-SOD1 expression in zebrafish. Mol Neurodegeneration 7, 44 (2012). https://doi.org/10.1186/1750-1326-7-44
- Amyotrophic lateral sclerosis (ALS)
- Motor neuron (MN)
- Cu2+/Zn2+ superoxide dismutase (SOD1)
- Neuromuscular junction