Figure 4

Microglia conditioned media from PS1 M146V KI mice impairs proliferation of AHNPCs. Representative image eGFP+ microglia (green) with ramified process in DG of PS1^M146V/+x Cx3Cr1^GFP/+ mice (A) and are negative for astrocytic marker, GFAP (red) (B). Bright field (C) and fluorescent (D) images of flow cytometry sorted primary eGFP+ cultures established from neonatal brains of PS1^M146V/+x Cx3Cr1^GFP/+ mice. Scale bar, 25 μm. Bright field image of AHNPCs grown as neurospheres from PS1^+/+, mice (E). Scale bar, 50 μm. (F) Proliferation achieved by PS1^+/+ AHNPCs following treatment with CM collected from IL-4 activated or PS1^+/+x Cx3Cr1^GFP/+or PS1^M146V/+x Cx3Cr1^GFP/+ microglia. Graph shows the extent of BrdU uptake in AHNPCs observed on days 1, 3 and 6. T-test; N = 3 independent cultures, with quadruplicates for each time point (m ± SEM). ** p < 0.01.