Figure 2

Electron micrographs of CLB and mitochondria in PD _Orig and PD _CLB cybrid lines. CLB in PD_Orig(A, C, E) and PD_CLB(B, D, F) lines typically had an electron dense, compact core (core in PD63_CLB is a double). The dense core is surrounded by a halo consisting of electron dense, heterogeneous aggregated material. Paired higher magnification images illustrate mitochondrial morphology in cells containing CLB. (A, B) The mitochondria in PD61_Orig were enlarged with reduced numbers of cristae and a pale matrix. Mitochondria in PD61_CLB were normal in appearance with a dense matrix and regular cristae. (C, D) Mitochondria in PD63_CLB were swollen with reduced numbers of fragmented cristae and a little to no matrix while mitochondria in PD63Orig were swollen with fragmented cristae and a pale matrix. (E, F) Mitochondria in PD67_Orig and PD67_CLB were rod-like, dense, and featured organized cristae. N= nucleus. Scale bar = 1μm.