Figure 5

Nucleoid content in PD _Orig and PD _CLB cybrid lines. (A, C, E) Representative images of nucleoids in the PD_Orig and PD_CLB pairs using PicoGreen and MTRed (green: DNA, red: mitochondria). Yellow puncta are nucleoids in mitochondria. Cells that lack nucleoids are red with no yellow puncta (A). The nucleoids in PD63_Orig and PD63_CLB(C) were larger in diameter. (B, D, F) Cells were scored for nucleoid content as either “null/low” or “high” (see Methods) and graphed as a percentage of total cells, n= 5; #, p<0.001. (A, B) Consistent with other data, PD61_CLB showed a significant increase in percent of cells that scored “high” and had fewer cells that scored “null/low” for nucleoid content compared to PD61_Orig. (C,D) Conversely, PD63_CLB showed an increase in cells that scored “null/low” and decrease in cells with “high” nucleoid density compared to PD63_Orig. (E,F) There was no difference in nucleoid content between PD67_CLB and PD67_Orig. Two-way ANOVA with Bonferroni multiple comparisons, n = 5; #, p<0.001. Scale bar=5μm.