Figure 7

Quantitative real-time PCR analysis of gene profiles in PD _Orig and PD _CLB cell lines. (A) Mitochondrial gene expression for four mtDNA encoded genes (ND2, CO3, ND4, 12srRNA) measured using RT-qPCR from cDNA and compiled to create a gene expression profile. Starting quantities were normalized to the geometric mean for each cell line and graphed as the average fold change from the mean from three CNTL cybrid lines (56, 64, 68) for each gene in this profile. (B) Mitochondrial gene copy numbers for the same four genes was assayed from gDNA and expressed as described above. (C) Mitochondrial biogenesis gene expression (PGC-1α, NRF1, TFAM, TFB1M) was measured from cDNA and expressed as described above. Student’s t-test, with Welch’s correction in the case of non-equal variances; *, p<0.05; **, p<0.01; #, p<0.001.