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Figure 1 | Molecular Neurodegeneration

Figure 1

From: Stem cell derived basal forebrain cholinergic neurons from Alzheimer’s disease patients are more susceptible to cell death

Figure 1

Differentiation of iPSCs into ventral forebrain neural progenitors. (A) 72 hours of SHH treatment of iPSCs (Control 1 line)-derived neural progenitors result in translocation of downstream effector Gli1 into the nucleus, Scale bar = 42 μm. Quantitative PCR shows up-regulation of the SHH downstream genes Ptch1 and Shh and the FGF8 downstream genes Etv5 and Spry2 after 72 hours of SHH and FGF8 treatment of neurospheres. Data are normalized to non-treated controls (n = 4, mean ± SEM, two-way ANOVA, p = 0.0003, F(1,32) = 17.73; Bonferroni multiple comparisons, *p < 0.05, **p < 0.01, ***p < 0.001, n indicates number of replicative experiments). Immunocytochemistry analysis of SHH and FGF8 treated neurospheres for forebrain marker (B) Forse1, ventral markers (C) Nkx2.1 and (E) Mash1 and dorsal marker (D) Pax6. Scale bars = 85 μm. Nuclei are stained with DAPI in blue. (F) Immunocytochemistry of SHH and FGF8 treated neurospheres shows expression of the telencephalic marker FoxG1 which co-localizes in the nucleus with DAPI.

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Molecular Neurodegeneration

ISSN: 1750-1326