Figure 5
Hdac3 cKO prevented apoptotic heterochromatin formation in TUJ-1 positive cells in the GCL 5 days post ONC. (A-C) The GCL of whole mounts from a Rosa26-LacZfl/fl control eye, a Rosa26-LacZfl/fl crushed eye, and an Hdac3fl/fl crushed eye stained with the TUJ-1 monoclonal antibody. Nuclear morphology was stained using DAPI. The DAPI staining of a representative cells is shown in the inset in the lower right corner of each panel. Nuclei of TUJ-1 positive cells in control (A) and Hdac3fl/fl crushed retinas (B) typically exhibited a normal appearance with minimal heterochromatin and a prominent nucleolus. (C) In Rosa26-LacZfl/fl retinas after ONC, however, some nuclei clearly showed condensed staining and were fragmented. (D) Graphical representation of cell counts indicating a significantly higher percentage of TUJ-1 positive apoptotic cells in Rosa26-LacZfl/fl crushed eyes when compared to crushed Hdac3 cKO and control eyes (*P ≤ 0.001). (Scale bars: 10 μm).