Open Access

Histone deacetylase 3 (HDAC3) plays an important role in retinal ganglion cell death after acute optic nerve injury

  • Heather M Schmitt1,
  • Heather R Pelzel2,
  • Cassandra L Schlamp1 and
  • Robert W Nickells1Email author
Molecular Neurodegeneration20149:39

https://doi.org/10.1186/1750-1326-9-39

Received: 27 May 2014

Accepted: 22 September 2014

Published: 28 September 2014

Abstract

Background

Optic nerve damage initiates a series of early atrophic events in retinal ganglion cells (RGCs) that precede the BAX-dependent committed step of the intrinsic apoptotic program. Nuclear atrophy, including global histone deacetylation, heterochromatin formation, shrinkage and collapse of nuclear structure, and the silencing of normal gene expression, comprise an important obstacle to overcome in therapeutic approaches to preserve neuronal function. Several studies have implicated histone deacetylases (HDACs) in the early stages of neuronal cell death, including RGCs. Importantly, these neurons exhibit nuclear translocation of HDAC3 shortly after optic nerve damage. Additionally, HDAC3 activity has been reported to be selectively toxic to neurons.

Results

RGC-specific conditional knockout of Hdac3 was achieved by transducing the RGCs of Hdac3 fl/fl mice with an adeno-associated virus serotype 2 carrying CRE recombinase and GFP (AAV2-Cre/GFP). Controls included similar viral transduction of Rosa26 fl/fl reporter mice. Optic nerve crush (ONC) was then performed on eyes. The ablation of Hdac3 in RGCs resulted in significant amelioration of characteristics of ONC-induced nuclear atrophy such as H4 deacetylation, heterochromatin formation, and the loss of nuclear structure. RGC death was also significantly reduced. Interestingly, loss of Hdac3 expression did not lead to protection against RGC-specific gene silencing after ONC, although this effect was achieved using the broad spectrum inhibitor, Trichostatin A.

Conclusion

Although other HDACs may be responsible for gene expression changes in RGCs, our results indicate a critical role for HDAC3 in nuclear atrophy in RGC apoptosis following axonal injury. This study provides a framework for studying the roles of other prevalent retinal HDACs in neuronal death as a result of axonal injury.

Keywords

HDACEpigeneticsRetinal ganglion cellNeuronal degenerationApoptosisChromatin remodelingDeacetylationHeterochromatin

Background

Glaucoma is a leading cause of blindness worldwide and is characterized by damage to the optic nerve [1, 2]. Increased intraocular pressure results in an increase in the strain surrounding the optic nerve head [3, 4], which is believed to precipitate focal damage to retinal ganglion cell (RGC) axons as they pass into the optic nerve [5]. Axogenic neurodegeneration precedes somatogenic neurodegeneration in the predicted pathophysiology of a majority of optic neuropathies, such as glaucoma [6]. Optic nerve crush (ONC) mimics these molecular events by inducing partial RGC axonal damage [7], and it is a widely accepted model of acute RGC injury that has been used to study intrinsic and extrinsic apoptotic mechanisms of RGC death [712].

The intrinsic mechanism of apoptosis involves activation and translocation of the pro-apoptotic protein BAX to the mitochondria following cellular injury. Oligomerization of BAX at the mitochondrial outer membrane releases cytochrome c from the mitochondria [13], which leads to activation of caspases and subsequent cell death [14]. The steps involving BAX mark the committed step of intrinsic apoptosis [15]. In a previous study, Bax-deficient RGCs remained viable up to at least 72 weeks post ONC, however, these cells exhibited many early stages of atrophy typical of wild type cells undergoing cell death [8]. This was particularly evident in structural and functional changes in RGC nuclei. The RGC nuclei were found to exhibit atrophic characteristics including nuclear shrinkage, histone H4 deacetylation, heterochromatin formation, and RGC-specific gene silencing soon after ONC [8, 10, 16].

Changes in the transcriptional profile of damaged neurons have been described in several models of neurodegeneration, including the down regulation (silencing) of normal gene expression and the increase in expression of stress-response and pro-apoptotic genes [10, 1726]. Transcriptional downregulation and initiation of the cell death mechanism in several cases of neuronal injury, including RGC death, were linked with epigenetic processes such as histone deacetylase (HDAC) activity [10, 18, 19, 2729]. Although most HDACs are found ubiquitously in tissues, class I HDAC isoforms 1, 2, 3, and 6 are found primarily in the cells of the inner nuclear layer and the ganglion cell layer (GCL) of the murine retina [10, 30]. A previous study showed that RGC gene silencing and RGC death were attenuated following ONC as a result of pretreatment with the broad spectrum HDAC inhibitor Trichostatin A (TSA) [10]. The same study demonstrated that HDAC3 translocated to the nucleus in concert with H4 deacetylation during RGC death [10]. These results suggested a potential role for HDAC3 in early RGC gene downregulation and global deacetylation events in RGC death following axonal injury. Other studies have also reported that HDAC3 is toxic to differentiated neurons, indicating an important role for HDAC3 molecular events in neurodegeneration [28, 31, 32].

Here we show that conditional knock out of Hdac3 in RGCs ameliorates global deacetylation and heterochromatin formation, while improving nuclear integrity and RGC viability following ONC. Interestingly, conditional knockout of Hdac3 does not prevent the downregulation of RGC-specific gene expression, even though TSA does. We interpret these data as indicating that a different class I HDAC may be responsible for global transcriptional regulation in the early stage of nuclear atrophy. Overall, the results indicate an important role for HDAC3 in the early events of neuronal intrinsic apoptosis and provide direction for dissecting the roles of other class I HDACs in the process of early transcriptional silencing during the RGC apoptotic program.

Results

Intravitreal AAV2-Cre/GFP injection transduces ganglion cell layer of mouse retina

To selectively ablate Hdac3 in RGCs, we transduced them with replication deficient AAV2 virus carrying a CRE expression cassette (AAV2-Cre/GFP). AAV2 has been reported to have selective tropism for RGCs [33]. To validate transduction of RGCs, we intravitreally injected C57BL/6-Rosa26 fl/fl mice containing either the LacZ or Tomato reporter gene and monitored reporter gene expression at times between 2 and 8 weeks of injection. In mice containing the Tomato reporter gene, fluorescence microscopy indicated that transduction of the ganglion cell layer plateaued by 4 weeks post intravitreal injection of AAV2-Cre/GFP (Additional file 1: Figure S1). It was also found that administration of 109 genome copies of AAV2-Cre/GFP was sufficient to achieve maximal transduction in the retina. In mice containing the LacZ reporter, X-Gal staining revealed that reporter gene expression occurred in the ganglion cell layer (GCL) after intravitreal injection of AAV2-Cre/GFP (Figure 1A-C). We detected AAV2-Cre/GFP transduction of cells present in the GCL; predominantly, in BRN3A labeled RGCs as shown by fluorescent microscopy of injected Rosa26-Tomato fl/fl mouse eyes (Figure 1D-E). The AAV2-Cre/GFP virus also transduced the occasional Müller cell (data not shown). No photoreceptors, or other neurons in the inner nuclear layer, were positively labeled for TOMATO or GFP.
Figure 1

AAV2-Cre/GFP transduces RGCs in Rosa26-LacZ fl/fl and Rosa26-Tomato fl/fl mice. (A-B) Retinal whole mounts from non-injected (A) and injected (B) eyes of Rosa26-LacZ fl/fl mice were taken 8 weeks following injection. X-Gal staining indicates global reporter gene expression in injected eyes. (C) Retinal section taken from an injected eye of Rosa26-LacZ fl/fl illustrates that X-Gal staining is restricted to cells of the ganglion cell layer (GCL) (Scale bar: 20 μm) and is not present in the outer nuclear layer (ONL) and inner nuclear layer (INL). (D) GFP fluorescence carried by AAV2-Cre/GFP is found in the RGC somas and axons (indicated by arrows) in a retinal whole mount (Scale bar: 10 μm). (E) AAV2-Cre/GFP tropism to RGCs in the GCL, and not to the inner plexiform layer (IPL) is shown by nuclear BRN3A (green), TOMATO (red), and DAPI (blue) co-labeling in a retinal section (arrowhead). (Scale bar: 4 μm).

Knockdown of Hdac3expression after intravitreal injection of AAV2-Cre/GFP

A previous study indicated that HDAC3 was recruited to the nuclei of RGCs following ONC, and that the mRNA abundance of both Hdac2 and Hdac3 increased at 1 and 3 days post ONC [10]. Here, we used fluorescence microscopy to monitor the expression of HDAC3 protein in cells of the GCL of Hdac3 fl/fl compared to Rosa26-LacZ fl/fl mice, 5 days post ONC. Fluorescence microscopy images of retinal sections demonstrated that both Rosa26-LacZ fl/fl and Hdac3 fl/fl mouse RGCs exhibited nuclear localization of HDAC2 (Figure 2A, B). Nuclear staining for HDAC3 was detected in the Rosa26-LacZ fl/fl (Figure 2C), but absent in Hdac3 fl/fl mouse RGCs (Figure 2D). Quantitative measurements of the Hdac3 mRNA also showed ~72% decrease in total retinal mRNA levels (Figure 2E, *P ≤ 0.05), while Western blotting for protein levels from individual retinas showed that 5/6 mice had reduced levels of HDAC3 in the injected eye (Figure 2F). Collectively, however, the overall levels of HDAC3 were not statistically significant. This discrepancy in mRNA and protein abundance may be due to the higher sensitivity of qPCR in comparison to Western blotting, or reflect differences in mRNA and protein stability in INL cells, which also express Hdac3. It was also found that HDAC3 protein levels increase ~350% by 5 days after ONC in Rosa26-LacZ fl/fl mice, consistent with previous reports of increased amounts of Hdac3 mRNA. Ablation of Hdac3 in RGCs abrogates this damage-induced increase (Figure 2G, *P ≤ 0.05).
Figure 2

Intravitreal AAV2-Cre/GFP injection achieved conditional knockout of the Hdac3 gene. (A-B) TEXAS RED labeling of HDAC2 in Rosa26-LacZ fl/fl and Hdac3 fl/fl AAV2-Cre/GFP injected eyes at 5 days post ONC illustrates nuclear localization of this HDAC in cells of the GCL of both retinas. (C-D) TEXAS RED labeling of HDAC3 in Rosa26-LacZ fl/fl and Hdac3 fl/fl AAV2-Cre/GFP injected eyes at 5 days post ONC demonstrates the lack of HDAC3 in the Hdac3 fl/fl RGCs (Scale bar: 15 μm). (E) QPCR was used to determine abundance of Hdac3 mRNA from pooled retinas of 5 Rosa26-LacZ fl/fl and Hdac3 fl/fl mice at 4 weeks following AAV2-Cre/GFP injection. In uncrushed retinas, the abundance of Hdac3 mRNA was significantly lower in Hdac3 cKO retinas compared to Rosa26-LacZ fl/fl retinas (*P ≤ 0.05). (F) Protein from individual retinas of AAV2-Cre/GFP injected (4-week incubation) and uninjected Hdac3 fl/fl mice showed an overall decrease in protein levels in Hdac3 cKO mice, although there was no significant difference in mean abundance (P > 0.05). (G) Pooled retinal protein from 5 AAV2-Cre/GFP injected Rosa26-LacZ fl/fl and Hdac3 fl/fl mice at 1 and 5 days post ONC were run on a Western blot. ONC results in an increase in HDAC3 accumulation in Rosa26-LacZ fl/fl mice by 5 days, but this response is significantly abrogated in Hdac3 fl/fl animals (*P ≤ 0.05) when comparing Rosa26-LacZ fl/fl and Hdac3 fl/fl mice at 5 days.

Hdac3cKO leads to amelioration of global deacetylation following RGC injury

Previously, it was shown that deacetylation of histone H4 occurred early in the apoptotic cascade initiated in RGCs as a result of ONC [8, 10]. Deacetylation was initiated within 24 hours following optic nerve damage, and peaked by 5 days. During the second and third days of this interval, HDAC3 was found localized to the nuclei of dying cells [10]. To investigate the specific role of HDAC3 in the process of H4 deacetylation, fluorescence microscopy was utilized to observe antibody staining of acetylated histone H4 (AcH4) in GCL nuclei at 5 days post ONC in Hdac3 +/+ and Hdac3 cKO mouse eyes (Figure 3A-E). Results from GCL cell counts indicated that Hdac3 cKO retinas retained comparable numbers of AcH4-positive nuclei in the GCL to that of untreated control retinas (P > 0.05). However, Rosa26-LacZfl/fl retinas that underwent ONC, exhibited a significant decrease of ~40% of positively labeled cells in the GCL (*P ≤ 0.05) (Figure 3F). This most likely represented loss of AcH4 in the nuclei of RGCs present in the GCL since RGCs make up ~50% of the population of neurons in this layer [34].
Figure 3

Hdac3 cKO ameliorated global deacetylation following RGC injury. (A-C) Retinal whole mounts were stained for AcH4 (red) in Rosa26-LacZ fl/fl and Hdac3 fl/fl AAV2-Cre/GFP injected and uninjected eyes at 5 days following optic nerve crush. The control retina was an uninjected and uncrushed Hdac3 fl/fl retina, which exhibited widespread AcH4 labeling (Scale bar: 10 μm). Nuclei absent of AcH4 staining were present only in the Rosa26-LacZ fl/fl retina after ONC. (D-E) Fluorescent microscopy of the retinal sections showed that the Hdac3 cKO GCL retained visibly more AcH4 labeled cells than the Rosa26-LacZ fl/fl GCL (Scale bar: 15 μm). (F) Cell counts in the GCL indicated Hdac3 cKO retinas at 5 days post ONC, retained AcH4 levels comparable to control retinas (P > 0.05), while Rosa26-LacZ fl/fl retinas exhibited about a 40% decrease in AcH4 labeled cells in the GCL compared to control retinas (*P ≤ 0.05).

Hdac3cKO blocks heterochromatin formation, nuclear envelope breakdown, and nuclear pore damage in RGCs following ONC

In addition to maximal histone deacetylation, 5 days after optic nerve crush also marks the point at which a significant number of affected cells exhibit the formation of heterochromatin [8]. To evaluate if HDAC3-mediated histone deacetylation correlates with heterochromatin formation at 5 days following ONC, cells in the GCL from Rosa26-Tomato fl/fl and Hdac3 fl/fl retinal sections were scored on a scale of 1–3 based on images from bright field microscopy (Figure 4). Cells in the GCL from Rosa26-Tomato fl/fl retinas scored significantly higher than in Hdac3 fl/fl retinas for fragmented and pyknotic nuclei (*P ≤ 0.05). To determine if the formation of pyknotic nuclei was restricted to RGCs, we also examined nuclear morphology of retinal whole mounts stained with DAPI and counter-stained with the TUJ-1 antibody, which has been reported to preferentially stain tubulin-rich RGCs [35]. Figure 5 shows that fragmented and pyknotic nuclei were significantly more numerous in Rosa26-Tomato fl/fl mice after ONC (*P ≤ 0.05), while levels remained at control retina levels in Hdac3 fl/fl mice. Nuclear morphology in TUJ-1 negative cells in the GCL was not significantly affected by Hdac3 cKO or ONC (data not shown).
Figure 4

Rosa26-Tomato fl/fl retinas exhibited significantly more pyknotic nuclei at 5 days following ONC when compared to Hdac3 fl/fl cKO and control retinas. (A) A section of the retina from a Rosa26-Tomato fl/fl mouse, 5 days after ONC, to show an exemplar of the heterochromatin scoring system. (B) Scoring data from masked observers is depicted by a bar graph. Significantly more heterochromatic and pyknotic nuclei were detected in Rosa26-Tomato fl/fl control crushed retinas when compared to uncrushed and Hdac3 cKO crushed retinas (*P < 0.05). Heterochromatin score of 1 = healthy cell with euchromatic nucleus and well-formed nucleolus, 2 = cell with partial apoptotic heterochromatin formation, and 3 = cell with completely heterochromatic (pyknotic) fragmented nuclei. (Scale bar: 10 μm).

Figure 5

Hdac3 cKO prevented apoptotic heterochromatin formation in TUJ-1 positive cells in the GCL 5 days post ONC. (A-C) The GCL of whole mounts from a Rosa26-LacZ fl/fl control eye, a Rosa26-LacZ fl/fl crushed eye, and an Hdac3 fl/fl crushed eye stained with the TUJ-1 monoclonal antibody. Nuclear morphology was stained using DAPI. The DAPI staining of a representative cells is shown in the inset in the lower right corner of each panel. Nuclei of TUJ-1 positive cells in control (A) and Hdac3 fl/fl crushed retinas (B) typically exhibited a normal appearance with minimal heterochromatin and a prominent nucleolus. (C) In Rosa26-LacZ fl/fl retinas after ONC, however, some nuclei clearly showed condensed staining and were fragmented. (D) Graphical representation of cell counts indicating a significantly higher percentage of TUJ-1 positive apoptotic cells in Rosa26-LacZ fl/fl crushed eyes when compared to crushed Hdac3 cKO and control eyes (*P ≤ 0.001). (Scale bars: 10 μm).

Transmission electron microscopy (TEM) was also used to visualize heterochromatin formation in GCL cells 5 days post ONC in AAV2-Cre/GFP injected Hdac3 fl/fl and Rosa26-Tomato fl/fl mice. TEM results demonstrated a higher incidence of early-apoptotic heterochromatic cells in the ONC Rosa26-Tomato fl/fl retinas in comparison to the ONC Hdac3 fl/fl retinas (Figure 6A-D). These results suggest that HDAC3 plays an important role in heterochromatin formation in RGCs following axonal injury from ONC.
Figure 6

Hdac3 cKO blocked heterochromatin formation in the GCL 5 days following ONC. (A-B) TEM images of cells in the GCL of a Rosa26-Tomatofl/fl control eye and AAV2-Cre/GFP injected eye, 5 days after ONC. Prominent heterochromatin formation is evident in the GCL of Rosa26-Tomatofl/fl crushed retinas. (C-D) TEM images of cells in the GCL of an Hdac3 fl/fl control eye and Hdac3 cKO crushed eye indicate no heterochromatin formation in the GCL as a result of ONC. Nuclei with characteristics of the morphological scores described in Figure 4 are indicated. N = nucleus, n = nucleolus, ne = nuclear envelope, and m = Müller endfoot. (Scale bar: 3 μm).

During the late stages of apoptosis, the nuclei of dying cells undergo nuclear envelope degradation and loss of nuclear pore integrity as a result of caspase-mediated lamin degradation. High magnification TEM imaging of nuclear envelopes revealed heterochromatin deposition adjacent to the inner surface of the inner membrane of cells in Rosa26-Tomato fl/fl mice. The perinuclear space between the inner and outer membranes also appeared expanded (Figure 7B). Nuclear envelope structure in Hdac3 fl/fl mice exhibited minimal deposition of heterochromatin and normal appearing pore structures. The inner and outer membranes, however, often appeared separated and wavy (Figure 7C).
Figure 7

Hdac3 cKO in the RGCs prevented the breakdown of the nuclear envelope and pore integrity following ONC. (A-C) TEM images were taken of nuclear envelopes from Hdac3 fl/fl control retinas, Rosa26-Tomato fl/fl crushed retinas, and Hdac3 fl/fl crushed retinas. The nuclear lamina is situated at the top of each micrograph. (A) Example of the well-defined inner and outer membranes of the nuclear envelope and normal pore structure of a healthy cell. (B) Example of the nuclear envelope from a cell in a Rosa26-Tomato fl/fl retina 5 days after ONC. The nucleus exhibits formation of electron-dense heterochromatin localized to the inner surface of the nuclear envelope, and the intermembrane space has expanded. (C) The nuclear envelope of a presumptive RGC in an Hdac3 fl/fl cKO mouse after ONC. The chromatin appears euchromatic and the nuclear envelop exhibits pore structures, although the apposition of the inner and outer membranes appears wavy. Arrowheads = nuclear pores, small arrows = nuclear envelope double membranes (ne), N = nucleus, and hc = heterochromatin. (Scale bar: 300 nm).

Gene silencing is not affected by Hdac3cKO retinas in RGCs post ONC

Previously, we found that the use of the broad-spectrum HDAC inhibitor, TSA, was effective in ameliorating RGC-specific Fem1c gene downregulation following ONC when administered intraperitoneally 24 hours prior to surgery [10]. To determine if HDAC3 activity regulates this response in dying RGCs, we quantified mRNA transcript levels in retinas from AAV2-Cre/GFP intravitreally injected Rosa26-Tomato fl/fl and Hdac3 fl/fl mice at 5 days post ONC. RGC specific genes of interest included Thy1, Sncg, Nrn1, Fem1c, and Nfl. The abundance of these mRNAs was lower in the ONC retinas than in the unilateral uncrushed control eyes in both the AAV2-Cre/GFP injected Rosa26-Tomato fl/fl and Hdac3 fl/fl mice (Figure 8). As a positive control, mRNA abundance was also quantified after pre-administration of TSA. Intraperitoneal injection of TSA, 24 hours prior to ONC, was able to attenuate the decrease in RGC-specific mRNA abundance when compared to uninjected and DMSO injected controls (*P ≤ 0.05). These results indicate that HDAC3 does not specifically regulate gene silencing following acute injury to RGCs, and they suggest that a different HDAC plays this role.
Figure 8

Gene silencing was not regulated by HDAC3 in RGCs post ONC. AAV2-Cre/GFP was injected intravitreally into Rosa26-Tomato fl/fl and Hdac3 fl/fl eyes 4 weeks prior to ONC. Additional mice were injected intraperitoneally with either DMSO or TSA, 24 hours prior to ONC. Changes in gene expression for each group of treated mice were compared to mice that had received no injection of AAV2-Cre/GFP or HDAC inhibitor (no injection group). Transcript abundance of ganglion cell genes Thy1, Sncg, Nrn1, Fem1c, and Nfl, measured by qPCR, showed marked decreases in non-injected and DMSO injected as well as AAV2-Cre/GFP injected Rosa26-Tomato fl/fl and Hdac3 fl/fl retinas at 5 days following ONC. No significant difference was observed among the change in transcript abundance between Hdac3 cKO and the Rosa26-Tomato fl/fl mice in this study (P ≥ 0.05). However, mice injected intraperitoneally with TSA 24 hours prior to ONC exhibited significantly higher levels of mRNA abundance (*P ≤ 0.05) of RGC specific genes at 5 days following ONC when compared to uninjected and DMSO injected mice.

RGC apoptotic death is attenuated in Hdac3cKO retinas post ONC

Previous studies have shown that broad-spectrum HDAC inhibitors such as TSA and valproic acid (VPA) prevent RGC degeneration in models of retinal ischemic injury and optic nerve crush [10, 30, 3638]. Others have shown that inhibition of HDAC1 and HDAC3 lead to RGC protection after neuronal injury [39]. HDAC3 has also been shown to be toxic to neurons in vitro [28, 39], and in models of degenerative disease such as Huntington’s disease, spinocerebellar ataxia type 7, and Friedrich’s ataxia [31, 40, 41]. Here, we assessed the effect of knockout of Hdac3 on RGC cell survival after ONC. Cell counts in the GCL indicated that at 2 weeks following ONC, Hdac3 cKO retinas had similar numbers (P > 0.05) of cells in the GCL when compared to its contralateral non-injected and uncrushed eye (Figure 9A). Conversely, injected Rosa26-Tomato fl/fl retinas that underwent crush lost a significant percentage of cells from the ganglion cell layer (*P ≤ 0.05), which was comparable to the rate of cell loss in non-injected Hdac3 fl/fl and Rosa26-Tomato fl/fl mice after ONC (data not shown). At 4 and 8 weeks post ONC, RGC numbers were significantly decreased (~20%) in the Hdac3 cKO retinas in comparison to contralateral control retinas (**P ≤ 0.05), but this was still significantly less cell loss compared to Rosa26-LacZ fl/fl retinas at all time points examined following ONC (*P ≤ 0.05) (Figure 9A). These results were similar to previous work that showed RGC protection by TSA treatment [16], indicating a critical role for HDACs, and especially HDAC3, in the process of RGC death.
Figure 9

RGC death was ameliorated in Hdac3 cKO retinas at 2 weeks but not at 4 and 8 weeks following ONC. (A) Hdac3 fl/fl mice injected with AAV2-Cre/GFP retained a significantly higher percentage of the RGCs by 2, 4, and 8 weeks post ONC in comparison to injected Rosa26-Tomato fl/fl mice (*P ≤ 0.05). Retinas from Hdac3 fl/fl mice injected with AAV2-Cre/GFP showed no significant cell loss at 2 weeks compared to contralateral eyes (P > 0.05), but did exhibit cell loss by 4 and 8 weeks post ONC (**P ≤ 0.05). (B) Hdac3 fl/fl mice injected with AAV2-Cre/GFP also exhibited lower total numbers of CASPASE-3 positive cells 5 days following ONC in comparison to Rosa26-LacZ fl/fl injected and crushed retinas (*P ≤ 0.05). (C-D) Examples of CASPASE-3 positive cells in (C) Rosa26-LacZ fl/fl and (D) Hdac3 fl/fl mice at 5 days post ONC. Images like these were used to collect the data graphed in (B). (Scale bar: 10 μm).

Previous work has indicated that caspase activation occurs at 3–5 days following RGC axonal injury [42]. Therefore, to determine whether Hdac3 cKO would ameliorate downstream apoptotic caspase activation, retinas from Hdac3 fl/fl and Rosa26-Tomato fl/fl injected mouse eyes were stained with antibody to activated CASPASE-3 at 5 days post ONC. It was illustrated that retinas with cKO of Hdac3 had significantly fewer positively labeled cells for activated CASPASE-3 in comparison to Rosa26-Tomato fl/fl retinas (*P ≤ 0.05) (Figure 9B-D). These results suggest that early apoptotic events mediated by HDAC3 activity might be necessary for initiating the activation of downstream events in the apoptotic pathway.

Discussion

The time course of RGC apoptosis can be temporally divided into phases of early cell and nuclear atrophy, initiation of BAX oligomerization, and late caspase and endonuclease activation leading to nuclear fragmentation. The main characteristics of the events of nuclear atrophy include gene silencing, global deacetylation, heterochromatin formation, and a decrease in nuclear structural integrity. Based on previous work showing neuronal toxicity of HDAC3, amelioration of RGC death with HDAC1 and HDAC3 inhibitors, and HDAC3 localization to the nuclei of RGCs prior to maximal histone H4 deacetylation during apoptosis [10, 28, 39], we sought to determine the role of HDAC3 in early RGC nuclear atrophy by analyzing each of the atrophic characteristics following ONC.

Here, we demonstrated that H4 deacetylation and heterochromatin formation were prevented in RGCs of Hdac3 cKO retinas at 5 days following ONC. Although nucleoli and nuclear pores remained normal appearing, we found that the RGCs of Hdac3 cKO retinas had wavy-appearing nuclear envelopes, which may reflect an intermediate stage of perinuclear swelling that was present in the nuclei of cells of crushed control mice. Alternatively, HDAC3 may play a role in maintaining the latticework that supports the inner nuclear membrane [43], by associating with lamin A/C (see below).

HDAC3 enzymatic activity, resulting in histone deacetylation and heterochromatin formation, are temporally situated early in the apoptotic program, because they still occur in Bax- deficient mice, where completion of apoptosis is effectively permanently blocked [8, 44]. It is not known, however, if these HDAC3-mediated changes are essential to activate further downstream events in the apoptotic pathway, such as caspase activation and nuclear fragmentation. Our results showing a correlation between HDAC3 activity and CASPASE-3 activation support a cause and effect relationship between these early events and later stages in the apoptotic pathway. Whether or not this relationship is linked to chromatin remodeling is not known at this time. HDAC3 may also play a role in modifying non-histone targets early in the apoptotic process. One such target may be the transcription factor p53. Acetylation and phosphorylation play a critical role in regulating p53 activity by altering cellular localization or binding affinity to specific targets [45]. Specifically, Chao and colleagues observed that HDACs could activate p53 by deacetylating lysine residue 317, leading to activation of pro-apoptotic gene expression such as Bbc3 (PUMA) and Bim[46]. Both of these BH3-only containing proteins of the Bcl2 family of genes function by modulating the activity of BAX to promote mitochondrial permeability leading to the release of cytochrome-c and the activation of the caspase cascade. Importantly, both of these proteins have been strongly implicated in regulating RGC apoptosis after optic nerve damage [42]. Separately, Brochier et al. [47] observed that inhibition of p53 DNA-binding and transcriptional activity in neurons was obtained by acetylation on lysines 381 and 382. The same post-translational modifications enhance p53 activity in cancer cells, however. If HDAC3 plays such a role in regulating the acetylation status of p53, this would provide a mechanism for the selective toxicity of this HDAC in differentiated neurons [28, 40, 48].

One of the events in nuclear atrophy not affected by Hdac3 deletion is the silencing of normal RGC gene expression. Previously, we showed that injection of broad-spectrum HDAC inhibitor, TSA, prior to ONC prevented silencing of the RGC specific gene promoter, Fem1c, in both acute and chronic (glaucomatous) models of axonal injury [10, 16]. We hypothesized that amelioration of gene silencing would occur with the conditional knockout of Hdac3 in RGCs of mice that later underwent ONC. However, knockout of Hdac3 did not lead to protection from gene silencing at 5 days following ONC, rather RGC specific gene transcript abundance also decreased similar to the change exhibited by wild type retinas. Conversely, TSA administration prior to ONC led to significant amelioration of RGC specific gene silencing in RGCs. These results, taken together with evidence that HDACs 1, 2, 3, and 6 localized in the retina [30], indicated a potential role for these other HDACs in the early event of gene silencing during RGC atrophy.

Several class I HDACs have been implicated in regulating gene transcription. An appropriate candidate for investigation may be HDAC2 due to it’s increased mRNA abundance in retinas at 1 day following ONC [10]. Previous work demonstrated that HDAC2 comprised approximately 35% of the total HDAC activity in the mouse retina and that retinas lacking HDAC2 underwent less retinal degeneration following ischemic insult [30]. The HDAC1/HDAC2 corepressor complex can be targeted to genes by transcription factors such as Sp1 and Sp3, playing a role in regulation of gene expression via chromatin remodeling [49].

A different mechanism of gene silencing, involving lamin-associated domains, was recently demonstrated in Drosophila S2 somatic cells, which exhibit silencing of a multi-genic testes gene cluster. This gene cluster is sequestered at the nuclear envelope as heterochromatin that interacts with the lamin protein complex. Domains, such as this one, are maintained by the activity of the Drosophila HDAC1 homolog, with the HDAC3 homolog playing an apparent auxiliary role [43]. Lamin-associated domains are increasingly recognized as a mechanism for repressing gene activity during development [50]. This phenomenon offers an interesting mechanism for gene silencing during cell death, and is consistent with one of the hallmarks of apoptosis, which is an initial accumulation of heterochromatin along the inner surface of the nuclear envelope [51, 52]. It is unknown if this accumulation of heterochromatin associates with lamin domains, or selectively involves aggregation of genomic DNA with actively transcribed genes that are targeted for silencing. This potential model warrants further investigation.

Although HDAC3, by itself, evidently does not seem to play a large role in early gene silencing, it may be a valuable molecular target for drug inhibitor therapy since knockout of Hdac3 expression has been shown to halt the progression of histone deacetylation and attenuate subsequent RGC death after axonal injury. An important consideration resulting from these experiments is that HDAC-mediated changes in RGCs can be segregated into very early events, likely associated with gene-silencing, and later events, associated with global chromatin remodeling. It is still relevant to address the mechanism of gene silencing, since RGC function is expected to rely on these cells having a normal profile of gene expression that defines cell identity and function. We anticipate that selective targeting of HDAC3 for protective therapy may yield living, but non-functional RGCs, because of their inability to express a genetic profile that identifies them as ganglion cells. Further investigation into functions and timing of different HDAC activities during the process of chromatin remodeling will provide insight into early epigenetic events that play a role in RGC apoptosis associated with optic neuropathies.

Conclusions

HDAC3 was found to play a major role in global deacetylation of histone H4, heterochromatin formation, and eventual cell death in RGCs that underwent axonal injury. However, deletion of HDAC3 did not affect gene silencing, even though the broad spectrum HDAC inhibitor was effective. This implies that other HDACs commonly found in the retina also play a role in early nuclear atrophic events during the intrinsic apoptotic program in RGCs.

Methods

Experimental animals, AAV2-Cre/GFP injection, and ONC

All mice were handled in accordance with the Association for Research in Vision and Ophthalmology statement for the use of animals for research, and experimental protocols were approved by the Institutional Animal Care and Use Committee of the University of Wisconsin. A random mixture of male and female C57BL/6 mice between the ages of 4–6 months, were used for experiments. The Rosa26-LacZ fl/fl mice were generously provided by Dr. Jing Zhang from the University of Wisconsin-Madison McArdle Laboratory for Cancer Research (Madison, Wisconsin). Rosa26-Tomato fl/fl mice were obtained from the Jackson Laboratory (Bar Harbor, Maine), and Hdac3 fl/fl mice [53] were obtained from Dr. Scott Hiebert at Vanderbilt University (Nashville, Tennessee, USA). The Rosa26 mice contain the loxP flanked PGK-Neo stop transcript found upstream of either Tomato or LacZ (βGEO) reporter transcript and were used to monitor for RGC transduction of the AAV2-Cre/GFP virus as well as Hdac3+/+ controls for viral injections (see below). Each treatment group contained at least 4 mice for all experiments except for heterochromatin formation analysis (n = 3). The C57BL/6 Hdac3 fl/fl mice contain loxP sites flanking exon 7 of the Hdac3 gene transcript [53].

Injections of 1 μL AAV2-Cre/GFP (equaling a total of 109 gc) were administered intravitreally 4 weeks prior to ONC. The 4-week time point was chosen after testing transduction efficiency at 1, 2, 4, and 8 weeks post AAV2-Cre/GFP injection showed optimal expression of Tomato starting at 4 weeks (Additional file 1: Figure S1). AAV2-Cre/GFP was obtained from Vector Biolabs (Philadelphia, PA, USA), and 1:10 dilution of stock AAV2-Cre/GFP was made in 5% glycerol in sterile phosphate buffered saline (PBS, 137 mM NaCl, 1.8 mM KH2PO4, and 10 mM Na2HPO4, pH 7.5) prior to injection. Intravitreal injections were conducted using a 10 μL Hamilton syringe with a 35G needle attached. A volume of 1 μL of diluted AAV2-Cre/GFP was injected over a period of 40 seconds, and the needle was held in the eye for at least 30 seconds before it was retracted. ONC was performed unilaterally using self-closing forceps to initiate degeneration of RGCs. Retinas were harvested at 5, 14, 28, and 56 days following ONC for analysis. Previously, we observed peak histone H4 deacetylation in the GCL by 5 days post-ONC [10]. Therefore, retinas were harvested at 5 days to assess mRNA abundance, histone H4 deacetylation, and heterochromatin formation. Retinas were collected at 14, 28, and 56 days post ONC for cell counts. Cell counts were obtained using a modification of the method described in Li et al. (2007) [54]. Briefly, digital images collected at 400× magnification were taken of each lobe of a retinal whole mount stained with DAPI. Cell numbers were determined in 24 separate 100 μm2 fields for each retina. Change in cell number for each experimental eye was calculated as a percentage of cell numbers in the corresponding control eye of each mouse.

β-galactosidase staining and bright field microscopy

β-galactosidase reporter expression was identified histochemically in retinal sections and whole mounts by X-Gal assay. X-gal staining solution was prepared ahead of time in the dark by adding N, N dimethylformamide to X-gal (0.02% Igepal, 0.01% sodium deoxycholate, 5 mM potassium ferricyanide, 5 mM potassium ferrocyanide, and 2 mM MgCl2 diluted in 0.1 M PBS [pH 7.3]). Mice were euthanized and eyes were collected and fixed in 4% paraformaldehyde in PBS at room temperature for 50 minutes. For whole mounts, the eye was then rinsed with PBS, and the anterior portion of the eye was removed, leaving an eyecup. The eyecup was then washed in PBS containing 2 mM MgCl2 and 2 μM CaCl2 and stained by incubation in staining solution containing 1 mg/mL X-gal at 37°C for 18 hours. After staining, the retina was dissected from the eyecup and whole mounted on glass slides. For sections, the eye was rinsed with PBS, and the anterior portion of the eye was removed, leaving an eyecup. The eyecup was then equilibrated in 30% sucrose overnight at 4°C before mounting in blocks of Tissue-Tek O.C.T. Compound from Fisher Scientific (Pittsburgh, PA) for cryosectioning (5 μm thick). Sections were then rinsed in PBS containing 2 mM MgCl2 and 2 μM CaCl2 and stained by incubation in staining solution containing 1 mg/mL X-gal at 37°C for 18 hours. Retinas were then washed in PBS and stained with nuclear fast red stain for 5 minutes. The slides were examined and photographed using an Olympus BX40 light microscope (Olympus America Inc., Center Valley, PA) and digital camera attachment.

Immunofluorescence

Indirect immunofluorescence on 5 μm thick frozen retinal sections and whole mounts was done as described previously [10]. Cryosections and whole mounts were mounted on Superfrost Plus microscope slides (Fisher Scientific) and rinsed in PBS. The sections were then blocked in 5% bovine serum albumin (BSA) in PBS for 3 hours at room temperature and later rinsed in PBS. Primary antibodies including polyclonal rabbit antibody to human HDAC3 (#sc-11417) and polyclonal rabbit antibody HDAC2 (#sc-7899) (both from Santa Cruz, Dallas, TX), polyclonal rabbit antibody to human AcH4 (#06-866) and monoclonal mouse primary to human BRN3A (#MAB1585) (both from EMD Millipore Inc., Billerica, MA), monoclonal mouse primary to human TUJ-1 (#ab14545) (AbCam, Cambridge, MA), and polyclonal rabbit primary antibody to human CASPASE-3 (#AF835) (R&D Systems, Minneapolis, MN) were used at 1:100 dilutions. Sections and whole mounts were incubated in primary antibody for 24–48 hours at 4°C and washed in PBS afterwards. Secondary antibodies used included goat anti-rabbit TEXAS RED (1:1,000) and goat anti-mouse FITC (1:1,000) (Jackson ImmunoResearch Laboratories, West Grove, PA). Sections and whole mounts were incubated in secondary antibody at room temperature in the dark for 2 hours and washed in PBS. All sections and whole mounts were counter-stained for 10 minutes with 4’, 6-diamidino-2-phenylindole (DAPI) and were washed in PBS. Finally, sections and whole mounts were mounted using Immumount mounting medium (Fisher Scientific) and coverslipped. Fluorescent images were obtained using a Zeiss Axioplan 2 Imaging microscope with Axiovision 4.6.3.0 software (Carl Zeiss MicroImaging Inc., Thornwood, NY).

Transmission Electron Microscopy (TEM)

Rosa26-Tomato fl/fl and Hdac3 fl/fl mouse eyes were injected with AAV2-Cre/GFP and after 4 weeks were subjected to ONC surgery. Animals were analyzed 5 days after ONC surgery. Enucleated eyes were immersed in 4% paraformaldehyde in 0.1 M Phosphate buffer (PB) for 5 minutes, after which the anterior chambers and lenses were dissected away from each eyecup. A small region of the superior eyecup was then removed and placed in 2.5% glutaraldehyde, 2% paraformaldehyde in PB overnight at 4°C. Tissues were postfixed in 1% osmium tetroxide in PB, dehydrated in ethanol, and embedded in Epon epoxy. Sections (60–90 nm) were cut, stained with 50% ethanoic uranyl acetate and Reynold’s lead citrate, and viewed using a Phillips CM120 transmission electron microscope (FEI Company, Hillsboro, OR).

Heterochromatin scoring analysis

Tissues processed for TEM were also sectioned for bright field microscopy. Thick (1 μm) sections were cut from epoxy embedded samples and stained with Richardson’s stain (methylene blue and azure blue). Sections were imaged using an Olympus BX40 light microscope and a digital camera attachment. Nuclear morphology of cells in the GCL was scored by 3 masked observers. A score of 1 indicated cells that exhibited healthy euchromatic nuclei with well-formed nucleoli; a score of 2 indicated cells that were partially heterochromatic; and a score of 3 indicated cells that had completely condensed pyknotic chromatin or fragmented nuclei.

Evaluation of transcript abundance in the retina by qPCR

Total retinal RNA was isolated from 5 pooled retinas at 5 days post ONC by acid-phenol extraction, and RNA was then DNase I treated (Promega, Madison, WI). First strand cDNA using reverse transcriptase and oligo (dT) was synthesized from 2 μg of isolated and purified total RNA [55]. The resulting cDNA was diluted 10-fold and 5 μl of cDNA was used for each qPCR reaction with SYBR Green PCR master mix (Applied Biosystems, Foster City, CA) and the appropriate RGC gene-specific primers as listed in the table of primer sequences (Table 1). Quantitative PCR was conducted on triplicate samples in each run using ABI 7300 Real Time PCR system (Applied Biosystems). Data were obtained from triplicate samples for each target cDNA. Absolute transcript quantification was based on a standard S16 curve run during the same reaction and the copy number was normalized to S16 ribosomal protein mRNA. The mRNA transcript values are expressed as the percent change from contralateral control eye to treatment eye. Data were reported as the mean ± SD of these differences.
Table 1

Primers for qPCR analysis

Gene name

Primer sequences

Size of product (bp)

Thy1

5’-CTTGCAGGTGTCCCGAGGGC-3’

379

5’-CTGAACCAGCAGGCTTATGC-3’

Sncg

5’-GACCAAGCAGGGAGTAACGG-3’

240

5’-TCCAAGTCCTCCTTGCGCAC-3’

Nrn1

5’-TTCACTGATCCTCGCGGTGC-3’

238

5’-TACTTTCGCCCCTTCCTGGC-3’

Nfl

5’-AGCACGAAGAGCGAGATGGC-3’

173

5’-TGCGAGCTCTGAGAGTAGCC-3’

S16

5’-CACTGCAAACGGGGAAATGG-3’

198

5’-TGAGATGGACTGTCGGATGG-3’

All primers were designed to span at least one intron. For each gene, the forward primer is shown first.

Western blot analysis

Western blot analysis was conducted on 5 pooled Tomato fl/fl and Hdac3 fl/fl mouse retinas from each treatment group described. Individual retinas harvested from Hdac3 cKO and control eyes were also analyzed. Retinal protein was loaded in triplicate with 50 μg per lane on 12% polyacrylamide gels and transblotted onto Immobilon P (Millipore, Inc., Billerica, MA). Membranes were probed for HDAC3, HDAC2, and ACTIN. Rabbit polyclonal antibodies were used at 1:1,000 for HDAC3 and HDAC2 and a goat monoclonal antibody was used at 1:250 for ACTIN (I-19) (cat# sc-1616) (Santa Cruz, CA). Firstly, the blots were incubated in donkey anti-goat secondary (1:10,000) conjugated to IRDye 800CW (cat# 926–32214), and after washing in PBS, incubated in goat anti-rabbit secondary (1:10,000) conjugated to IRDye 680RD (cat# 926–68071) (LICOR, Lincoln, NE). Images were scanned and analyzed using the Odyssey Clx (LICOR). Band fluorescence was quantified using Image Studio software, and data were normalized to the ACTIN loading control on each blot.

Statistical analysis

Data were collected from a minimum of 4 independent samples in all experiments except for analysis of heterochromatin formation (n = 3), and shown as the mean ± standard deviation in all experiments except for cell counts, where data was shown as the mean ± standard error. All statistical analyses were performed using either the Student’s t-test with statistical significance set at P ≤ 0.05 for comparison of two groups or ANOVA with Bonferroni adjustments with statistical significance set at P ≤ 0.05 for comparison of multiple groups.

Abbreviations

ONC: 

Optic nerve crush

RGC: 

Retinal ganglion cell

GCL: 

Ganglion cell layer

INL: 

Inner nuclear layer

IPL: 

Inner plexiform layer

ONL: 

Outer nuclear layer

HDAC: 

Histone deacetylase

AcH4: 

Acetylated histone 4

TEM: 

Transmittance electron microscopy

βGeo: 

β-Galactosidase and neomycin phosphotransferase fusion reporter protein

X-gal: 

(BCIG) for 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside

TSA: 

Trichostatin A

VPA: 

Valproic acid

PBS: 

Phosphate-buffered saline

BSA: 

Bovine serum albumin

DMSO: 

Dimethyl sulfoxide

DAPI: 

4’, 6-diamidino-2-phenylindole

cKO: 

Conditional knockout

AAV2-Cre/GFP: 

Adeno-associated virus serotype 2 carrying Cre recombinase and green fluorescent protein

PGK-neo: 

Phosphoglycerate kinase I neomycin resistance gene

gc: 

Genome copies

N: 

Nucleus

n: 

Nucleolus

ne: 

Nuclear envelope

m: 

Müller endfoot

hc: 

Heterochromatin

WT: 

Wild-type.

Declarations

Acknowledgements

This work was supported by National Eye Institute Grant R01 EY012223 (RWN) and Vision Science CORE Grant P30 EY016665 (Department of Ophthalmology and Visual Sciences, University of Wisconsin), and unrestricted funding from Research to Prevent Blindness, Inc. (Department of Ophthalmology and Visual Sciences, University of Wisconsin).

Authors’ Affiliations

(1)
Department of Ophthalmology and Visual Sciences, School of Medicine and Public Health, University of Wisconsin-Madison
(2)
Department of Biology, University of Wisconsin-Whitewater

References

  1. Nickells RW: Retinal ganglion cell death in glaucoma: the how, the why, and the maybe. J Glaucoma. 1996, 5 (5): 345-356.View ArticlePubMedGoogle Scholar
  2. Quigley HA, Nickells RW, Kerrigan LA, Pease ME, Thibault DJ, Zack DJ: Retinal ganglion cell death in experimental glaucoma and after axotomy occurs by apoptosis. Invest Ophthalmol Vis Sci. 1995, 36 (5): 774-786.PubMedGoogle Scholar
  3. Fortune B, Burgoyne CF, Cull GA, Reynaud J, Wang L: Structural and functional abnormalities of retinal ganglion cells measured in vivo at the onset of optic nerve head surface change in experimental glaucoma. Invest Ophthalmol Vis Sci. 2012, 53 (7): 3939-3950. 10.1167/iovs.12-9979.PubMed CentralView ArticlePubMedGoogle Scholar
  4. Gardiner SK, Fortune B, Wang L, Downs JC, Burgoyne CF: Intraocular pressure magnitude and variability as predictors of rates of structural change in non-human primate experimental glaucoma. Exp Eye Res. 2012, 103: 1-8.PubMed CentralView ArticlePubMedGoogle Scholar
  5. Nickells RW, Howell GR, Soto I, John SW: Under pressure: cellular and molecular responses during glaucoma, a common neurodegeneration with axonopathy. Annu Rev Neurosci. 2012, 35: 153-179. 10.1146/annurev.neuro.051508.135728.View ArticlePubMedGoogle Scholar
  6. Schwartz M, Yoles E, Levin LA: ‘Axogenic’ and ‘somagenic’ neurodegenerative diseases: definitions and therapeutic implications. Mol Med Today. 1999, 5 (11): 470-473. 10.1016/S1357-4310(99)01592-0.View ArticlePubMedGoogle Scholar
  7. Schwartz M: Optic nerve crush: protection and regeneration. Brain Res Bull. 2004, 62 (6): 467-471. 10.1016/S0361-9230(03)00076-5.View ArticlePubMedGoogle Scholar
  8. Janssen KT, Mac Nair CE, Dietz JA, Schlamp CL, Nickells RW: Nuclear atrophy of retinal ganglion cells precedes the bax-dependent stage of apoptosis. Invest Ophthalmol Vis Sci. 2013, 54 (3): 1805-1815. 10.1167/iovs.11-9310.PubMed CentralView ArticlePubMedGoogle Scholar
  9. Levkovitch-Verbin H, Harris-Cerruti C, Groner Y, Wheeler LA, Schwartz M, Yoles E: RGC death in mice after optic nerve crush injury: oxidative stress and neuroprotection. Invest Ophthalmol Vis Sci. 2000, 41 (13): 4169-4174.PubMedGoogle Scholar
  10. Pelzel HR, Schlamp CL, Nickells RW: Histone H4 deacetylation plays a critical role in early gene silencing during neuronal apoptosis. BMC Neurosci. 2010, 11: 62-10.1186/1471-2202-11-62.PubMed CentralView ArticlePubMedGoogle Scholar
  11. Schlamp CL, Johnson EC, Li Y, Morrison JC, Nickells RW: Changes in Thy1 gene expression associated with damaged retinal ganglion cells. Mol Vis. 2001, 7: 192-201.PubMedGoogle Scholar
  12. Li Y, Schlamp CL, Nickells RW: Experimental induction of retinal ganglion cell death in adult mice. Invest Ophthalmol Vis Sci. 1999, 40 (5): 1004-1008.PubMedGoogle Scholar
  13. Desagher S, Osen-Sand A, Nichols A, Eskes R, Montessuit S, Lauper S, Maundrell K, Antonsson B, Martinou JC: Bid-induced conformational change of Bax is responsible for mitochondrial cytochrome c release during apoptosis. J Cell Biol. 1999, 144 (5): 891-901. 10.1083/jcb.144.5.891.PubMed CentralView ArticlePubMedGoogle Scholar
  14. Creagh EM, Martin SJ: Caspases: cellular demolition experts. Biochem Soc Trans. 2001, 29 (Pt 6): 696-702.View ArticlePubMedGoogle Scholar
  15. Chang LK, Putcha GV, Deshmukh M, Johnson EM: Mitochondrial involvement in the point of no return in neuronal apoptosis. Biochimie. 2002, 84 (2–3): 223-231.View ArticlePubMedGoogle Scholar
  16. Pelzel HR, Schlamp CL, Waclawski M, Shaw MK, Nickells RW: Silencing of Fem1cR3 gene expression in the DBA/2 J mouse precedes retinal ganglion cell death and is associated with histone deacetylase activity. Invest Ophthalmol Vis Sci. 2012, 53 (3): 1428-1435. 10.1167/iovs.11-8872.PubMed CentralView ArticlePubMedGoogle Scholar
  17. Ahmed F, Brown KM, Stephan DA, Morrison JC, Johnson EC, Tomarev SI: Microarray analysis of changes in mRNA levels in the rat retina after experimental elevation of intraocular pressure. Invest Ophthalmol Vis Sci. 2004, 45 (4): 1247-1258. 10.1167/iovs.03-1123.View ArticlePubMedGoogle Scholar
  18. Blalock EM, Geddes JW, Chen KC, Porter NM, Markesbery WR, Landfield PW: Incipient Alzheimer’s disease: microarray correlation analyses reveal major transcriptional and tumor suppressor responses. Proc Natl Acad Sci U S A. 2004, 101 (7): 2173-2178. 10.1073/pnas.0308512100.PubMed CentralView ArticlePubMedGoogle Scholar
  19. Cha JH: Transcriptional dysregulation in Huntington’s disease. Trends Neurosci. 2000, 23 (9): 387-392. 10.1016/S0166-2236(00)01609-X.View ArticlePubMedGoogle Scholar
  20. Chou AH, Yeh TH, Ouyang P, Chen YL, Chen SY, Wang HL: Polyglutamine-expanded ataxin-3 causes cerebellar dysfunction of SCA3 transgenic mice by inducing transcriptional dysregulation. Neurobiol Dis. 2008, 31 (1): 89-101. 10.1016/j.nbd.2008.03.011.View ArticlePubMedGoogle Scholar
  21. Duke DC, Moran LB, Pearce RK, Graeber MB: The medial and lateral substantia nigra in Parkinson’s disease: mRNA profiles associated with higher brain tissue vulnerability. Neurogenetics. 2007, 8 (2): 83-94. 10.1007/s10048-006-0077-6.View ArticlePubMedGoogle Scholar
  22. Ferraiuolo L, Heath PR, Holden H, Kasher P, Kirby J, Shaw PJ: Microarray analysis of the cellular pathways involved in the adaptation to and progression of motor neuron injury in the SOD1 G93A mouse model of familial ALS. J Neurosci. 2007, 27 (34): 9201-9219. 10.1523/JNEUROSCI.1470-07.2007.View ArticlePubMedGoogle Scholar
  23. Soto I, Oglesby E, Buckingham BP, Son JL, Roberson ED, Steele MR, Inman DM, Vetter ML, Horner PJ, Marsh-Armstrong N: Retinal ganglion cells downregulate gene expression and lose their axons within the optic nerve head in a mouse glaucoma model. J Neurosci. 2008, 28 (2): 548-561. 10.1523/JNEUROSCI.3714-07.2008.View ArticlePubMedGoogle Scholar
  24. Sugars KL, Rubinsztein DC: Transcriptional abnormalities in Huntington disease. Trends Genet. 2003, 19 (5): 233-238. 10.1016/S0168-9525(03)00074-X.View ArticlePubMedGoogle Scholar
  25. Yang Z, Quigley HA, Pease ME, Yang Y, Qian J, Valenta D, Zack DJ: Changes in gene expression in experimental glaucoma and optic nerve transection: the equilibrium between protective and detrimental mechanisms. Invest Ophthalmol Vis Sci. 2007, 48 (12): 5539-5548. 10.1167/iovs.07-0542.View ArticlePubMedGoogle Scholar
  26. de Lima S, Koriyama Y, Kurimoto T, Oliveira JT, Yin Y, Li Y, Gilbert HY, Fagiolini M, Martinez AM, Benowitz L: Full-length axon regeneration in the adult mouse optic nerve and partial recovery of simple visual behaviors. Proc Natl Acad Sci U S A. 2012, 109 (23): 9149-9154. 10.1073/pnas.1119449109.PubMed CentralView ArticlePubMedGoogle Scholar
  27. Frew AJ, Johnstone RW, Bolden JE: Enhancing the apoptotic and therapeutic effects of HDAC inhibitors. Cancer Lett. 2009, 280 (2): 125-133. 10.1016/j.canlet.2009.02.042.View ArticlePubMedGoogle Scholar
  28. Bardai FH, D’Mello SR: Selective toxicity by HDAC3 in neurons: regulation by Akt and GSK3beta. J Neurosci. 2011, 31 (5): 1746-1751. 10.1523/JNEUROSCI.5704-10.2011.PubMed CentralView ArticlePubMedGoogle Scholar
  29. Lagali PS, Picketts DJ: Matters of life and death: the role of chromatin remodeling proteins in retinal neuron survival. J Ocul Biol Dis Infor. 2011, 4 (3): 111-120. 10.1007/s12177-012-9080-3.PubMed CentralView ArticlePubMedGoogle Scholar
  30. Fan J, Alsarraf O, Dahrouj M, Platt KA, Chou CJ, Rice DS, Crosson CE: Inhibition of HDAC2 protects the retina from ischemic injury. Invest Ophthalmol Vis Sci. 2013, 54 (6): 4072-4080. 10.1167/iovs.12-11529.PubMed CentralView ArticlePubMedGoogle Scholar
  31. Duncan CE, An MC, Papanikolaou T, Rugani C, Vitelli C, Ellerby LM: Histone deacetylase-3 interacts with ataxin-7 and is altered in a spinocerebellar ataxia type 7 mouse model. Mol Neurodegener. 2013, 8 (1): 42-10.1186/1750-1326-8-42.PubMed CentralView ArticlePubMedGoogle Scholar
  32. McQuown SC, Wood MA: HDAC3 and the molecular brake pad hypothesis. Neurobiol Learn Mem. 2011, 96 (1): 27-34. 10.1016/j.nlm.2011.04.005.PubMed CentralView ArticlePubMedGoogle Scholar
  33. Hellstrom M, Ruitenberg MJ, Pollett MA, Ehlert EM, Twisk J, Verhaagen J, Harvey AR: Cellular tropism and transduction properties of seven adeno-associated viral vector serotypes in adult retina after intravitreal injection. Gene Ther. 2009, 16 (4): 521-532. 10.1038/gt.2008.178.View ArticlePubMedGoogle Scholar
  34. Schlamp CL, Montgomery AD, Mac Nair CE, Schuart C, Willmer DJ, Nickells RW: Evaluation of the percentage of ganglion cells in the ganglion cell layer of the rodent retina. Mol Vis. 2013, 19: 1387-1396.PubMed CentralPubMedGoogle Scholar
  35. Zhang XM, Li Liu DT, Chiang SW, Choy KW, Pang CP, Lam DS, Yam GH: Immunopanning purification and long-term culture of human retinal ganglion cells. Mol Vis. 2010, 16: 2867-2872.PubMed CentralPubMedGoogle Scholar
  36. Crosson CE, Mani SK, Husain S, Alsarraf O, Menick DR: Inhibition of histone deacetylase protects the retina from ischemic injury. Invest Ophthalmol Vis Sci. 2010, 51 (7): 3639-3645. 10.1167/iovs.09-4538.PubMed CentralView ArticlePubMedGoogle Scholar
  37. Biermann J, Boyle J, Pielen A, Lagreze WA: Histone deacetylase inhibitors sodium butyrate and valproic acid delay spontaneous cell death in purified rat retinal ganglion cells. Mol Vis. 2011, 17: 395-403.PubMed CentralPubMedGoogle Scholar
  38. Biermann J, Grieshaber P, Goebel U, Martin G, Thanos S, Di Giovanni S, Lagreze WA: Valproic acid-mediated neuroprotection and regeneration in injured retinal ganglion cells. Invest Ophthalmol Vis Sci. 2010, 51 (1): 526-534. 10.1167/iovs.09-3903.View ArticlePubMedGoogle Scholar
  39. Chindasub P, Lindsey JD, Duong-Polk K, Leung CK, Weinreb RN: Inhibition of histone deacetylases 1 and 3 protects injured retinal ganglion cells. Invest Ophthalmol Vis Sci. 2013, 54 (1): 96-102. 10.1167/iovs.12-10850.PubMed CentralView ArticlePubMedGoogle Scholar
  40. Bardai FH, Verma P, Smith C, Rawat V, Wang L, D’Mello SR: Disassociation of histone deacetylase-3 from normal huntingtin underlies mutant huntingtin neurotoxicity. J Neurosci. 2013, 33 (29): 11833-11838. 10.1523/JNEUROSCI.5831-12.2013.PubMed CentralView ArticlePubMedGoogle Scholar
  41. Xu C, Soragni E, Chou CJ, Herman D, Plasterer HL, Rusche JR, Gottesfeld JM: Chemical probes identify a role for histone deacetylase 3 in Friedreich’s ataxia gene silencing. Chem Biol. 2009, 16 (9): 980-989. 10.1016/j.chembiol.2009.07.010.PubMed CentralView ArticlePubMedGoogle Scholar
  42. Harder JM, Libby RT: Deficiency in Bim, Bid and Bbc3 (Puma) do not prevent axonal injury induced death. Cell Death Differ. 2013, 20 (1): 182-10.1038/cdd.2012.119.PubMed CentralView ArticlePubMedGoogle Scholar
  43. Milon BC, Cheng H, Tselebrovsky MV, Lavrov SA, Nenasheva VV, Mikhaleva EA, Shevelyov YY, Nurminsky DI: Role of histone deacetylases in gene regulation at nuclear lamina. PLoS One. 2012, 7 (11): e49692-10.1371/journal.pone.0049692.PubMed CentralView ArticlePubMedGoogle Scholar
  44. Semaan SJ, Li Y, Nickells RW: A single nucleotide polymorphism in the Bax gene promoter affects transcription and influences retinal ganglion cell death. ASN Neuro. 2010, 2 (2): e00032-PubMed CentralView ArticlePubMedGoogle Scholar
  45. Knights CD, Catania J, Di Giovanni S, Muratoglu S, Perez R, Swartzbeck A, Quong AA, Zhang X, Beerman T, Pestell RG, Avantaggiati ML: Distinct p53 acetylation cassettes differentially influence gene-expression patterns and cell fate. J Cell Biol. 2006, 173 (4): 533-44. 10.1083/jcb.200512059.PubMed CentralView ArticlePubMedGoogle Scholar
  46. Chao C, Wu Z, Mazur SJ, Borges H, Rossi M, Lin T, Wang JY, Anderson CW, Appella E, Xu Y: Acetylation of mouse p53 at lysine 317 negatively regulates p53 apoptotic activities after DNA damage. Mol Cell Biol. 2006, 26 (18): 6859-6869. 10.1128/MCB.00062-06.PubMed CentralView ArticlePubMedGoogle Scholar
  47. Brochier C, Dennis G, Rivieccio MA, McLaughlin K, Coppola G, Ratan RR, Langley B: Specific acetylation of p53 by HDAC inhibition prevents DNA damage-induced apoptosis in neurons. J Neurosci. 2013, 33 (20): 8621-8632. 10.1523/JNEUROSCI.5214-12.2013.View ArticlePubMedGoogle Scholar
  48. Chen Y, He R, Chen Y, D’Annibale MA, Langley B, Kozikowski AP: Studies of benzamide- and thiol-based histone deacetylase inhibitors in models of oxidative-stress-induced neuronal death: identification of some HDAC3-selective inhibitors. ChemMedChem. 2009, 4 (5): 842-852. 10.1002/cmdc.200800461.View ArticlePubMedGoogle Scholar
  49. He S, Khan DH, Winter S, Seiser C, Davie JR: Dynamic distribution of HDAC1 and HDAC2 during mitosis: association with F-actin. J Cell Physiol. 2013, 228 (7): 1525-1535. 10.1002/jcp.24311.View ArticlePubMedGoogle Scholar
  50. Puckelwartz MJ, Depreux FF, McNally EM: Gene expression, chromosome position and lamin A/C mutations. Nucleus. 2011, 2 (3): 162-167. 10.4161/nucl.2.3.16003.PubMed CentralView ArticlePubMedGoogle Scholar
  51. Kerr JF, Wyllie AH, Currie AR: Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer. 1972, 26 (4): 239-257. 10.1038/bjc.1972.33.PubMed CentralView ArticlePubMedGoogle Scholar
  52. Kerr JF: Shrinkage necrosis: a distinct mode of cellular death. J Pathol. 1971, 105 (1): 13-20. 10.1002/path.1711050103.View ArticlePubMedGoogle Scholar
  53. Bhaskara S, Knutson SK, Jiang G, Chandrasekharan MB, Wilson AJ, Zheng S, Yenamandra A, Locke K, Yuan JL, Bonine-Summers AR, Wells CE, Kaiser JF, Washington MK, Zhao Z, Wagner FF, Sun ZW, Xia F, Holson EB, Khabele D, Hiebert SW: Hdac3 is essential for the maintenance of chromatin structure and genome stability. Cancer Cell. 2010, 18 (5): 436-447. 10.1016/j.ccr.2010.10.022.PubMed CentralView ArticlePubMedGoogle Scholar
  54. Li Y, Semaan SJ, Schlamp CL, Nickells RW: Dominant inheritance of retinal ganglion cell resistance to optic nerve crush in mice. BMC Neurosci. 2007, 8: 19-10.1186/1471-2202-8-19.PubMed CentralView ArticlePubMedGoogle Scholar
  55. Doherty PJ, Huesca-Contreras M, Dosch HM, Pan S: Rapid amplification of complementary DNA from small amounts of unfractionated RNA. Anal Biochem. 1989, 177 (1): 7-10. 10.1016/0003-2697(89)90003-1.View ArticlePubMedGoogle Scholar

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