Fig. 3

E4 mice have lower energy expenditure and fail to increase oxygen consumption following a high carbohydrate diet. a-f Female E3 and E4 mice were housed individually for 48 h with ad libitum access to normal chow (a-c) or a high carbohydrate diet (HCD) (d-f) and energy expenditure (EE), VCO₂ and VO₂ were measured. Dark cycles are indicated in grey with light cycles in white. Light cycles were used for calculating averages of EE, VCO₂ and VO₂ (shown to the right) (***P < 0.0001, ****P < 0.00001, unpaired t-test, two-tailed; E3 n = 13, E4 n = 20). j Activity and k food consumption during light cycles were averaged for E3 and E4 mice (E3 n = 10, E4 n = 14). l Analysis of covariance was performed by separately correlating average EE and body weight for E3 and E4 mice. (Spearman correlation r = 0.86, ***P < 0.001)