Fig. 3

Chronic treatment with LNK-754 decreases BACE1 in dystrophic neurites in 5XFAD mice. Confocal immunofluorescence microscopy showing cortical (A) and hippocampal (B) regions from 5-month-old 5XFAD mice treated with vehicle, LNK-754, and lonafarnib immunostained for Aβ42 (white), LAMP1 (green) and BACE1 (red). Scale bar, 50 μm. Quantification of BACE1 covered area in the cortex (*p = 0.026) (C). Quantification of BACE1 and LAMP1 co-localization in the cortex (*p = 0.017) (D) (Vehicle, 188; LNK-754, 177; lonafarnib,168 plaques analyzed) and hippocampus (*p = 0.018) (E) (Vehicle, 87; LNK-754, 68; lonafarnib, 108 plaques analyzed). Vehicle, n = 11 (5 males, 6 females); LNK-754, n = 10 (4 males, 6 females); lonafarnib n = 10 (4 males, 6 females). Triangles represent males and circles represent females. 1-way ANOVA with Tukey’s post-hoc multiple comparisons test was performed