NF-κB is a critical mediator of post-mitotic senescence in oligodendrocytes and subsequent white matter loss

Schlett, Judith Stefanie; Mettang, Melanie; Skaf, Aladdin; Schweizer, Pavel; Errerd, Alina; Mulugeta, Ephraim Alemayehu; Hein, Tabea Melissa; Tsesmelis, Konstantinos; Tsesmelis, Miltiadis; Büttner, Ulrike F. G.; Wendt, Heinrich; Abaei, Alireza; Rasche, Volker; Prex, Vivien; Nespoli, Ester; Alami, Najwa Ouali; Tews, Daniel; Walther, Paul; Yilmazer-Hanke, Deniz; Oswald, Franz; Dimou, Leda; Wirth, Thomas; Baumann, Bernd

doi:10.1186/s13024-023-00616-5

Research article
Open access
Published: 17 April 2023

NF-κB is a critical mediator of post-mitotic senescence in oligodendrocytes and subsequent white matter loss

Judith Stefanie Schlett¹^na1,
Melanie Mettang¹^na1,
Aladdin Skaf²,
Pavel Schweizer¹,
Alina Errerd¹,
Ephraim Alemayehu Mulugeta¹,
Tabea Melissa Hein¹,
Konstantinos Tsesmelis¹,
Miltiadis Tsesmelis¹,
Ulrike F. G. Büttner¹,
Heinrich Wendt¹,
Alireza Abaei³,
Volker Rasche³,
Vivien Prex¹,
Ester Nespoli²,
Najwa Ouali Alami⁴,
Daniel Tews⁵,
Paul Walther⁶,
Deniz Yilmazer-Hanke⁴,
Franz Oswald⁷,
Leda Dimou²,
Thomas Wirth¹^na2 &
…
Bernd Baumann ORCID: orcid.org/0000-0003-4327-811X¹^na2

Molecular Neurodegeneration volume 18, Article number: 24 (2023) Cite this article

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Abstract

Background

Inflammaging represents an accepted concept where the immune system shifts to a low-grade chronic pro-inflammatory state without overt infection upon aging. In the CNS, inflammaging is mainly driven by glia cells and associated with neurodegenerative processes. White matter degeneration (WMD), a well-known process in the aging brain, manifests in myelin loss finally resulting in motor, sensory and cognitive impairments. Oligodendrocytes (OL) are responsible for homeostasis and maintenance of the myelin sheaths, which is a complex and highly energy demanding process sensitizing these cells to metabolic, oxidative and other forms of stress. Yet, the immediate impact of chronic inflammatory stress like inflammaging on OL homeostasis, myelin maintenance and WMD remains open.

Methods

To functionally analyze the role of IKK/NF-κB signaling in the regulation of myelin homeostasis and maintenance in the adult CNS, we established a conditional mouse model allowing NF-κB activation in mature myelinating oligodendrocytes. IKK2-CA^{PLP−CreERT2} mice were characterized by biochemical, immunohistochemical, ultrastructural and behavioral analyses. Transcriptome data from isolated, primary OLs and microglia cells were explored by in silico pathway analysis and validated by complementary molecular approaches.

Results

Chronic NF-κB activation in mature OLs leads to aggravated neuroinflammatory conditions phenocopying brain inflammaging. As a consequence, IKK2-CA^{PLP−CreERT2} mice showed specific neurological deficits and impaired motoric learning. Upon aging, persistent NF-κB signaling promotes WMD in these mice as ultrastructural analysis revealed myelination deficits in the corpus callosum accompanied by impaired myelin protein expression. RNA-Seq analysis of primary oligodendrocytes and microglia cells uncovers gene expression signatures associated with activated stress responses and increased post mitotic cellular senescence (PoMiCS) which was confirmed by elevated senescence-associated β-galactosidase activity and SASP gene expression profile. We identified an elevated integrated stress response (ISR) characterized by phosphorylation of eIF2α as a relevant molecular mechanism which is able to affect translation of myelin proteins.

Conclusions

Our findings demonstrate an essential role of IKK/NF-κB signaling in mature, post-mitotic OLs in regulating stress-induced senescence in these cells. Moreover, our study identifies PoMICS as an important driving force of age-dependent WMD as well as of traumatic brain injury induced myelin defects.

Graphical Abstract

Background

Aging includes a decline in brain function leading to deficits in motor, sensory and cognitive performance. Imaging studies in humans and other mammals document a volume shrinkage of the aging brain with reduction in both grey and white matter substance, and post mortem brain analyses identified widespread myelin defects in aged humans [1,2,3,4,5,6].

The causes include degenerative changes of myelin sheaths with subsequent axonal loss, overall compromising the conduction velocity and leading to dysfunctional neuronal circuits [7]. Myelination and homeostatic maintenance of myelin sheaths critically depend on the function of both mature OLs and their progenitor cells (called OPCs or NG2-glia), a process coupled to high energy demand. OL possess only limited capacity of self-protection and are prone to mitochondrial dysfunction leading to oxidative stress, an issue, often observed in the context of neuroinflammatory diseases [8]. Thus, aging-dependent white matter loss in humans and in mouse models argues for a stress-induced deregulation of myelination and/or deficits in the maintenance of healthy myelin sheaths [4,5,6, 9].

The IKK/NF-κB signaling pathway is a well-known mediator of inflammatory and stress responses [10,11,12], also including the regulation of diverse neuroinflammatory reactions [13,14,15]. Various age-related diseases are all linked to NF-κB mediated inflammatory processes [16, 17] and chronic neuroinflammation is increasingly recognized as a central pathogenic mechanism in neurodegeneration [18]. IKK/NF-κB signaling is acting as a major driver of aging processes in different organs including the brain [19, 20], and is also involved in or even causes cellular senescence [21,22,23] and regulates the SASP secretome [24, 25]. Moreover, progeroid mouse models based on permanent genotoxic stress share an upregulation of NF-κB signaling [26, 27]. Conversely, genetic or pharmacological inhibition of NF-κB delays the onset of phenotype development or mitigates the age-related disease burden indicating a promising translational role of NF-κB in aging processes [28,29,30]. However, the underlying cellular and molecular mechanisms as well as the cellular specificity of NF-κB initiated aging processes in the brain still remain elusive. In addition, IKK/NF-κB signaling is a major regulator of inflammaging, a persistent, sterile and gradually progressing inflammatory condition also acting in the CNS [31]. The driving force of inflammaging are different forms of cellular stress like the accumulation of molecules summarized as damage-associated molecular patters (DAMPs), which are all competent to activate the IKK/NF-κB pathway. However, the mechanistic interrelations connecting inflammation, cellular stress, OL function and WMD still remain elusive.

While developmental myelination is independent of NF-κB function, we asked whether IKK/NF-κB signaling is involved in the aging-dependent regulation of myelin homeostasis and maintenance and how NF-κB mediated inflammaging in OLs may contribute to WMD.

Results

NF-κB activation in Oligodendrocytes initiates inflammatory conditions in the CNS

To elucidate the role of NF-κB in the regulation of myelin homeostasis and maintenance we established a conditional OL-specific IKK/NF-κB gain-of-function model named IKK2-CA^{PLP−CreERT2} allowing tamoxifen-dependent transgene expression in around 60% of CC1-positive OLs in adult mice (Fig. S1a-d). Immunoblot analysis revealed significant upregulation of IKK2-CA transgene and eGFP reporter gene expression in different CNS areas of adult IKK2-CA^{PLP−CreERT2} mice 3 weeks after tamoxifen treatment (3 wpi), and p65 phosphorylation confirmed functional activation of the canonical IKK/NF-κB signaling pathway (Fig. 1a and Fig. S1e). As consequence various neuroinflammation marker genes, like Ccl2 or Ccl5 were significantly upregulated in different areas (Fig. 1b and Fig. S1f), while other genes like iNos, IL1β, or Lcn2 show a rather selective upregulation in the OL-rich corpus callosum (Fig. S1g). Notably, we detected reactive gliosis in response to NF-κB-mediated gene expression in OLs as prominent Iba1 and GFAP staining identified persisting microgliosis and rather transient astrogliosis in the corpus callosum when analyzed at 3 and 20 wpi (Fig. 1c-f).

In the healthy adult brain, homeostasis of NG2-glia/OPCs and OLs is strictly controlled [32] but gets disturbed in models of brain injury and neurodegenerative disease [33]. Interestingly, the number of mature CC1 + OLs in the corpus callosum significantly increased at 20 wpi in IKK2-CA^{PLP−CreERT2} mice but was found similar at 3 wpi whereas the absolute number of OLs with GFP reporter gene expression did not change between 3 and 20 wpi (Fig. 1g and h). Conversely, an overall increased number of NG2-glia/OPCs was determined at both time points (Fig. 1i and j), but mitotic NG2-glia/OPCs (Pdgfrα/Ki67) were only present at 3 wpi (Fig. S1h and i). Importantly, the lack of NG2/GFP double positive cells (Fig. S1j) argues against transgene activity in NG2-glia/OPCs thereby excluding direct NF-κB-mediated effects on NG2 cells. Similar, the transgene is not active in the peripheral nervous system as GFP expression was absent in sciatic nerve tissue (Fig. S1k). Thus, NF-κB activation in mature, myelinating CC1 + OLs is sufficient to induce reactive gliosis and non-cell-autonomous expansion of NG2-glia/OPCs leading to an increase in CC1 + OLs number. This is further supported by the absence of apoptotic OLs determined by TUNEL-assay at 3 and 20 wpi (data not shown).

IKK2-CA^{PLP−CreERT2}animals develop neurological deficits and decreased motoric learning

IKK2-CA^{PLP−CreERT2} mice show a normal weight development until 19 wpi, however thereafter weight gain is significantly decreased compared to controls (Fig. S2a). To address the functional consequences of chronic IKK/NF-κB signaling in OLs, we conducted long-term behavioral analyses including Neurological Severity Score [34] (NSS), rotarod, beam walk, ladder walk as well as grip strength measurement. Significant neurological deficits were discovered already 3 wpi, and over the whole course of 40 weeks IKK2-CA^{PLP−CreERT2} animals showed higher scores in the NSS than control animals (Fig. 2a). When animals were introduced to the rotarod task immediately after transgene activation, there was no difference in the motoric performance of IKK2-CA^{PLP−CreERT2} and control animals (Fig. 2b) until 40 wpi (Fig. S2b). However, IKK2-CA^{PLP−CreERT2} animals trained for the first time at the 20 wpi timepoint failed to improve their performance over the course of the trials (Fig. 2c). A similar picture appeared in the beam walking test with one group trained continuously for 20 weeks (“trained”) and another group that was introduced to the task for the first time at 20 wpi (“untrained”). Both groups managed the easier tasks (12 mm square and a 17 mm round beam) similarly, however for the more difficult tasks (5 mm square and 11 mm round beam) untrained transgenic animals needed significantly longer to cross the beam. This deficit was significantly attenuated, when animals had been continuously trained for 20 weeks (Fig. 2d and e) implying that aged IKK2-CA^{PLP−CreERT2} animals have a decreased capacity to learn new motor skills. Next, the ladder walk test was employed to assess motor coordination [35]. IKK2-CA^{PLP−CreERT2} animals exhibited significantly more missteps both in the irregular as well as in the regular ladderwalk with even greater difference in the latter (Fig. 2f and g). Importantly, we could not determine any obvious cerebellar atrophy or degeneration of purkinje cells (Fig. S2c-e), as previously observed in mouse model with chronic NF-κB activation in astrocytes [36]. Finally, grip strength measurement revealed a significant lower performance already early after transgene activation which does not further increase with aging (Fig. 2h).

These data suggest, that IKK2-CA^{PLP−CreERT2} mice develop a specific motoric phenotype resembling a disruption of white matter integrity linked with poor motor performance.

IKK/NF-κB activation in oligodendrocytes promotes white matter loss

Intact myelin sheath formation and maintenance of its integrity is an important prerequisite for learning new and complex motor skills [37]. Therefore, we examined the myelination status in IKK2-CA^{PLP−CreERT2} mice. Interestingly, we identified a modest but significant degeneration of the corpus callosum at different bregma points using Luxol Fast Blue (LFB) staining at 20 wpi whereas no obvious difference was determined at 3wpi (Fig. 3a and b, Fig. S3g-j). Ex-vivo MRI analysis of brains at 20 wpi revealed decompacted white matter tracts, hypointensities and thinning of the corpus callosum (Fig. S3a-c). Subsequently, electron microscopy was exploited for ultrastructural analysis of myelin sheaths revealing that g-ratio, appearance and variance in the number and diameter of myelinated axons was not altered at 3 wpi and 20 wpi. (Fig. 3c-f, Fig. S3d and e). However, the overall percentage of myelinated axons was significantly reduced in IKK2-CA^{PLP−CreERT2} animals at 20 wpi (Fig. 3g). At this timepoint, the degree of axonal myelination just remained at the level of 3 wpi whereas the known aging-dependent increase in myelination was detected in controls. Usually, CNS axons become myelinated at a diameter size larger than around 0.2 μm [38]. This principle gets disturbed in the IKK2-CA^{PLP−CreERT2} model as we found a significant increase in the size of unmyelinated axons at 20 wpi (Fig. 3h und S3f). However, we did not observe obvious early axonal pathology by SMI32 staining (Fig. S2f).

Myelination and myelin homeostasis associated gene expression is only marginally affected by NF-κB activation in oligodendrocytes

To gain further insight into this specific myelination defect, we next conducted gene and protein expression analysis of myelination-relevant genes. Whole cortex tissue revealed no significant changes in Mbp, Plp, Mog and Mobp mRNA expression at 3 wpi and 20 wpi. In contrast, Mbp, Plp1 and Mog protein levels were clearly decreased especially at 20 wpi in the cortex (Fig. 4a-h). To understand the mechanism(s) underlying this decline in myelin proteins in detail, we isolated O4⁺ OLs from IKK2-CA^{PLP−CreERT2} animals and control littermates at 3 and 20 wpi. The number of isolated O4⁺ OLs was similar in both groups, and subsequent RNA and protein analyses revealed strong expression of the IKK2-CA transgene and the GFP reporter gene in IKK2-CA^{PLP−CreERT2} O4⁺ cells (Fig. S4a-e). FACS analysis showed that the overall amount of O4⁺ cells within the MACS purified cell population was around 70%. There was a prominent enrichment of GFP⁺ O4 cells, but not all O4 purified cells express GFP similar to the immunohistological analyses shown before (Fig. 1) and according to the restricted PLP-promotor activity of the driver line [39]. Only OLs with an active PLP-promotor (around 60% of CC1 cells) can undergo recombination within the 3-week time frame of tamoxifen application. All OLs differentiating from NG2-glia/OPCs later in the absence of tamoxifen cannot recombine anymore thereby reducing the number of GFP⁺ cells within the isolated O4⁺ fraction. FACS staining of O4-bead sorted cells revealed that around 75% of O4 ⁺ cells are also GalC positive and all cells with high O4 expression are positive for the mature OL marker MOG (Fig. S4g).

RNA sequencing analysis of purified O4⁺ OLs revealed 412/181 significantly down and 1121/602 significantly upregulated genes at 3 and 20 wpi, respectively (Fig. S4h and i). Distinct differences between cells of IKK2-CA^{PLP−CreERT2} and control mice show a clear separation of the groups in the principal component analysis (Fig. 4j). Consistent with the decreased number of GFP⁺ O4⁺ OLs, NF-κB target gene expression at 20 wpi stays significantly upregulated, however overall level decreased compared to 3 wpi (Fig. S4k). Similar to whole cortex tissue analysis (Fig. 4a-d), genes encoding structural proteins involved in myelin sheath formation (GO: 0,043,209) like Mog, Mbp, Cnp, Mobp and Plp1 were slightly affected in O4⁺ OLs from IKK2-CA^{PLP−CreERT2} mice without reaching overall significance (Fig. S4j). Other genes connected to myelination, myelin assembly and maintenance, regulation of myelination and paranodal junction assembly (GO:0,042,552) including Cst7, Tlr2, Igf1, Epb41l3, Hes5 and Cntn2 also revealed a mild deregulation pattern without reaching overall significance (Fig. 4j).

These findings imply that IKK/NF-κB signaling in OLs does not directly interfere with transcriptional regulation of genes relevant for myelination but rather affects different cellular processes which as a consequence result in myelin protein loss.

IKK/NF-κB activation in mature oligodendrocytes induces a specific stress and senescence program eliciting translational arrest

To follow the above hypothesis, we applied GSEA to our RNAseq data and detected especially at 3 wpi a prominent enrichment in pathways related to cellular stress, senescence, SASP factors and p53 signaling in IKK2-CA^{PLP−CreERT2} OLs (Fig. S5a-c). This includes profound marker genes involved in oxidative stress like Hmox 1 or Txn 1, DNA damage response genes like Brca1, marker genes for ER Stress like ATF4 and GADD34 and cellular senescence related genes like GADD45, p38 (MAPK11/MAPK12), p16, p21 or p53 (Fig. 5a and b). Recently the term post mitotic cellular senescence (PoMiCS) evolved to include post mitotic, terminally differentiated cells [40]. To test whether the senescence gene expression signature leads to senescent OLs we monitored SA-β-galactosidase activity, a biomarker for cellular senescence. Importantly, we found a strong increase in SA-β-galactosidase positive OLs isolated from IKK2-CA^{PLP−CreERT2} animals compared to controls (Fig. 5c and d). Cellular senescence is typically associated with changes in cell morphology and also characterized by a highly active metabolism with increased glycolytic activity [41]. When primary O4⁺ OLs were cultured and differentiated ex vivo we observed differences in size and morphology compared to O4⁺ OLs isolated from control animals (Fig. 5e). In addition, we found the mitochondrial respiration rate (oxidative consumption rate, OCR, Fig. 5f) as well as glycolysis (extracellular acidification rate, ECAR, Fig. 5g) significantly upregulated in IKK2-CA^{PLP−CreERT2} OLs (3 wpi).

Translational control represents a paradox during senescence of proliferating cells as selective translation of. SASP is promoted while global translation is repressed [42] (Fig. S5c). To characterize the reduction of myelin proteins in IKK2-CA^{PLP−CreERT2} OLs we investigated the regulation of translation in O4⁺ OLs. An important factor involved in this process is the eucaryotic initiation factor 2 (eIF2). Phosphorylation of the α-subunit of eIF2 by stress-activated kinases renders eIF2 function to act as a negative regulator of translation. The overall outcome is a decrease in global protein synthesis and enhancement of translation of stress-related mRNAs (Fig. S5d) [43]. In line with this, prolonged IKK/NF-κB signaling in OLs leads to a significant increase in phosphorylated eIF2α, while absolute levels of eIF2α do not change (Fig. 5h and i). Furthermore, RNA-Seq analysis revealed a significant upregulation of stress response genes like ATF4 by fourfold and the upstream kinase PERK by 1.6-fold. Consistent with increased protein degradation, we detected a significant enrichment of genes involved in proteasome function (Fig. S5e).

Thus, IKK2 activation in post-mitotic OLs is capable of inducing a specific stress response program leading to premature senescence characterized by suppressed myelin protein translation and prominent upregulation of SASP factors.

IKK/NF-κB activation in oligodendrocytes promotes microglia polarization towards aged white matter microglia

We identified prominent reactive microgliosis (Fig. 1e and f) in our IKK2-CA^{PLP−CreERT2} model suggesting non-cell-autonomous effects upon NF-κB activation in OLs. Therefore, we purified Cd11b⁺ microglia cells next to the O4⁺ cell isolation (Fig. S4a and Fig. S6a) from the same mice, and detected a significant expansion of isolated microglia cells in IKK2-CA^{PLP−CreERT2} mice compared to control animals (Fig. 6a).

RNA-Sequencing analysis of purified CD11b⁺ microglia revealed 1282/1954 and 187/416 up- and downregulated genes at 3 and 20 wpi, respectively (Fig. S6b and c). PCA shows a distinct clustering of the different groups (Fig. 6b) and gene expression profiling points to an inflammatory polarization state [44] of IKK2-CA^{PLP−CreERT2} derived microglia (Fig. 6c). Interestingly, gene expression analysis reveals a prominent overlap with aged white matter-associated microglia (WAM; S6d) and also uncovers a cellular senescence signature in IKK2-CA^PLP−Cre microglia [45] (3 wpi, Fig. S6e). In line with this, IKK2-CA^{PLP−CreERT2} microglia (3 wpi) exhibit a decrease in oxidative phosphorylation and an increase in glycolytic activity in seahorse analysis (Fig. 6d and e), and developed a rather bushy shape morphology (Fig. 6f). While the cell count of isolated microglia stayed significantly elevated in IKK2-CA^{PLP−CreERT2} mice, gene expression gets dampened at 20 wpi arguing for quantitative and/or qualitative changes in paracrine signaling components released by IKK2-CA^{PLP−CreERT2} OLs. According to this, we identified several, potentially NG2-glia/OPC growth promoting factors markedly upregulated in IKK2-CA^{PLP−CreERT2} microglia like Cspg4, Pdgfa, Hgf1 and Nrp1 at 3 wpi, which are normalized again at 20 wpi (Table S5).

Traumatic brain injury promotes senescence of oligodendrocytes and decline in MBP protein

To address the translational relevance of our findings, we focused on traumatic brain injury (TBI) as there is strong evidence from patients that TBI induces white matter lesions. However, it is not known whether PoMiCS of OLs contributes to WMD in TBI. To this end, we conducted experimental TBI, in particular the most common closed head injury (CHI), and performed bulk RNAseq analysis of insult tissue taken from the ipsilateral brain hemisphere of wildtype TBI and sham treated animals. PCA uncovered strong changes in gene expression in response to TBI (data not shown). Subsequent GO term analysis revealed an obvious overlap in differentially expressed genes previously found in O4⁺ OLs isolated from IKK2-CA^{PLP−CreERT2} mice and bulk insult tissue post TBI. These genes are associated with stress response, cellular senescence and SASP factors (Tables S1, S2 and S3). Interestingly, myelination-associated genes were slightly but significantly upregulated by TBI (Table S4) whereas isolated OLs rather show a mild downregulation. We then performed TBI and purified O4⁺ OLs from the ipsi- and contralateral side of NF-κB reporter gene mice [46] and subsequent FACS analysis confirmed NF-κB activation in OLs post TBI (Fig. 7a and b). Importantly, we identified a clear enrichment of SA-β-galactosidase positive OLs isolated from the ipsilateral side indicating TBI-induced senescence (Fig. 7c and d). Consistent with that immunohistology shows a downregulation of MBP in parallel to Lcn2 upregulation, a well-known NF-κB target gene and SASP factor (Fig. 7e and f). Thus, PoMiCS of OLs is a valid principle relevant for TBI pathogenesis and potentially acting in WMD.

Discussion

Our study provides important insights how IKK/NF-κB signaling contributes to stress regulation in myelinating oligodendrocytes. We demonstrate that NF-κB in mature OLs is able to induce cell-autonomous and non-cell-autonomous stress reactions overall triggering WMD. NF-κB activation in OLs promotes microgliosis, astrogliosis and expansion of NG2-glia/OPCs leading to increased levels of OLs. Our data favor the hypothesis that age-associated white matter loss does not directly depend on the demise of OLs. Rather it is initiated by NF-κB-induced PoMiCS, which presents as a dysfunctional OL state characterized by deficits in myelin homeostasis. As an immediate consequence, steady-state myelinogenesis and/or myelin maintenance becomes impaired. In the long-term this culminates in white matter degeneration (graphical abstract).

Myelination and motor learning are mutually dependent on each other. Physiological myelination is indispensable for learning new motor tasks [37], whereas exercising of motor tasks is able to promote myelination or remyelination [47,48,49]. As a consequence, white matter defects come along with deficits in motor learning as the fine-tuned cortical ensemble becomes disturbed [50]. Our data indicate that chronic IKK/NF-κB signaling in a subset of CC1 + OLs is sufficient to impair myelin homeostasis and thereby forcing behavioral deficits. Concurrently, the increased proliferation of NG2-glia and the higher number of CC1 + OLs found in IKK2-CA^{PLP−CreERT2} mice argue for a ‘hypermyelination-like’ phenotype. This might represent a potential safeguard mechanism to replace dysfunctional, stressed or senescent OLs. Our RNA sequencing data show that NF-κB activation in OLs induces paracrine effects as differential clustering of IKK2-CA^{PLP−CreERT2} microglia was apparent in PCA. Furthermore, their gene expression profile reflects a rather pro-inflammatory M1-like signature, also known to arise in the context of CNS injury [51, 52]. We therefore consider it likely that microglia cells get instructed by NF-κB activated OLs to promote NG2-glia/OPC proliferation. Indeed, we identified several potentially NG2-glia/OPC growth promoting factors upregulated in IKK2-CA^{PLP−CreERT2} microglia like Cspg4, Pdgfa, Hgf1 and Nrp1. While Cspg4 (encoding Ng2) is upregulated in microglia upon acute brain injuries [53], Nrp1 (Neuropilin-1) was attributed to NG2-glia/OPC expansion in the white matter in an age-dependent manner, and its deletion was found to impair myelin homeostasis. Nrp1 can activate the platelet derived growth factor receptor alpha (Pdgfrα), similar to its consensus ligand, Pdgfa, to promote NG2-glia/OPC proliferation [54]. Hepatocyte growth factor (Hgf) was found to be secreted by microglia in spinal cord lesions in the remitting phase of experimental autoimmune encephalopathy [55].

Our results support a model where NF-κB activation in mature OLs induces a gene expression program including the release of secreted factors (SASP and others) which results in an injury like polarization program in microglia that is able to promote non-cell-autonomous NG2-glia/OPC proliferation. However, the additional OLs derived thereof might not be fully terminally differentiated and functionally integrated due to the lack of co-stimulating signals. Indeed, in brain injury, different factors like environmental enrichment and stimulation of neuronal activity were found to increase the integration capability of newly matured OLs [56,57,58,59]. Thus, the additional OLs in the IKK2-CA^{PLP−CreERT2} model arise from an activated backup system but rather stay dysfunctional due to the lack of an immediate need.

Recently, PoMiCS was postulated as a potential protective measure relevant for tissues like the CNS, possessing a high proportion of terminally differentiated post-mitotic cells [9]. The OL lineage represents a specific case as senescence might be applied to both mitotic NG2-glia/OPCs and post-mitotic OLs. Senescent NG2-glia/OPCs fail to differentiate into mature myelinating OLs thereby reducing the remyelination process at advanced age in mouse models of MS [60, 61]. Similar, senescent NG2-glia/OPCs were associated with Alzheimer´s Disease (AD) and treatment with senolytic drugs improved disease symptoms [62]. In contrast, aging of mature OLs seems to impair the maintenance of myelin sheaths, probably due to cellular PoMiCS [9, 63]. The fact, that OLs can accumulate DNA damage (e.g., by oxidative stress) thereby activating senescence markers like p53 and p16 supports the idea of stress-induced senescence in mature OLs [8, 9]. Importantly, a recent study provided comprehensive evidence that mature OLs are able to undergo a senescent program upon genetically induced DNA damage [64], supporting the critical role of PoMiCS to OL dysfunction.

Studies on NF-κB function in OLs mostly focused on remyelination efficacy in a disease context and importantly, only limited time windows of young ages were analyzed [65,66,67]. Inflammation was rather considered a consequence but not a cause of pathological demyelination [68, 69]. A different IKK2-CA mouse model (CNP/Cre x R26Stop^flikk2ca) also shows that OLs’ viability is not affected by NF-κB but also did not impair myelination until the age of 8 weeks [70]. In contrast to our model, NF-κB activation in this study remained very low as typical NF-κB target genes were not significantly induced.

Our RNAseq analysis of O4⁺ OLs isolated from IKK2-CA^{PLP−CreERT2} mice clearly revealed gene expression profiles resembling an obvious NF-κB signature together with stress responses and cellular senescence. This includes classical NF-κB target genes, hallmarks of cellular senescence, and stress response markers indicative for ER stress, oxidative stress and DNA damage. OLs are exceptionally sensitive to oxidative stress and this vulnerability increases with age as adult-born OLs ensheath a higher number of different axonal segments [71]. O4⁺ sorted and ex vivo cultivated OLs from IKK2-CA^{PLP−CreERT2} mice showed an increased glycolytic activity and oxidative phosphorylation indicating a direct effect of NF-κB on OL metabolism. However, a scenario of vicious cycle formation is also conceivable whereat enhanced ROS formation due to increased oxidative phosphorylation accelerates stress responses. Consistent with our findings, senescent cells are known to undergo metabolic reprogramming to tailor their energy demand [72]. The detailed mechanisms how PoMiCS in OLs contributes to white matter degeneration upon aging is not resolved. A recent report [73] hypothesized that senescence in OLs might be independent of p16. Our RNA-Seq data revealed a clear upregulation of p16 and p21 in OLs derived from IKK2-CA^{PLP−CreERT2} mice. Oxidative stress (increased levels of 8-OHdG) and SA-β-Galactosidase activity were found post mortem [74] in human aged white matter tissue suggesting that PoMiCS is a valid concept possibly preceding white matter loss in humans. We also provide evidence that conditions known to result in progressive WMD like TBI, activates NF-κB and senescence in OLs. The molecular underpinnings of white matter changes in TBI are not well established so far. TBI induces multiple stress pathways which differ in quality (e.g., iron overload upon bleedings, edema formation) and quantity and may overlap only to a limited degree (e.g., oxidative stress, neuroinflammation). Therefore, aging-driven senescence and TBI-induced senescence processes most likely differ in quantity (and kinetics) as the expression level of relevant genes (e.g., Spp1, C3, Sepine1, Hmox1) evidently vary between the IKK2-CA^{PLP−CreERT2} model and TBI.

In addition to the persisting microgliosis, our RNA seq data argue for an abundance of a CD11b⁺/CD11c⁺ microglia population in IKK2-CA^{PLP−CreERT2} mice. This subset of microglia cells has been found enriched in the white matter and associated with aging-dependent accumulation of myelin debris [75]. Single cell RNA-sequencing identified prominent gene signatures associated with phagosome function, antigen processing and presentation compared to homeostatic microglia in this white matter associated microglia (WAM) [76]. WAM exhibit high Trem2 expression, an important factor for phagocytosis of myelin debris [76, 77]. Importantly, this specific expression profile is even more pronounced in our model supporting the idea that NF-κB activation in OLs can initiate premature aging conditions in both OLs and microglia.

The role of stress response pathways in the regulation of myelination dynamics is controversially discussed. The integrated stress response (ISR) is a protective innate mechanism that can reduce the cytotoxic impact of aberrant inflammatory conditions. It is initiated via the phosphorylation of eIF2α (a subunit of the eIF2 protein complex) by different upstream kinases like PERK, finally repressing initiation of translation. Modulation of eIF2 activity has been shown to regulate the ISR and prolonging ISR through genetic or pharmacological approaches confirmed a protective role of eIF2α phosphorylation in experimental autoimmune encephalomyelitis [78,79,80]. We found that NF-κB activation in O4⁺ OLs induces a stress response similar to ISR including phosphorylation of eIF2α. This renders OLs in a senescent and dysfunctional state finally promoting WMD.

Our data reveal an important molecular link to vanishing white matter disease (VWMD), one of the most prevalent hereditary white matter diseases in childhood. Patients suffer from cerebral white matter degeneration associated with increased numbers of NG2-glia/OPCs similar to our IKK2-CA^{PLP−CreERT2} model. VWMD is caused by mutations in one of the five eukaryotic translation initiation factor genes, eucaryotic initiation factor 2B (eIF2B), which are involved in protein synthesis regulation [81]. The eIF2B complex functions as guanine nucleotide exchange factor (GEF) for eIF2 and VWMD loss-of-function mutations overall show a decrease in eIF2B GEF activity leading to repression of translation similar to eIF2α phosphorylation. Although eIF2B is a global regulator of protein translation, glia cells appear selectively vulnerable, as in VWMD brains of both mice and patients, ISR deregulation was observed in myelinating OLs and astrocytes.

Our findings provide an integrated mechanistic view how senescence of mature OLs can promote white matter loss under chronic neuroinflammatory conditions but also upon acute traumatic insults. Cell-type-specific modulation of NF-κB activity might open up new therapeutic strategies to reduce or delay age-dependent and post-traumatic WMD. Importantly, small molecule inhibitors of the ISR known to restore protein synthesis by counteracting p-eIF2α activity, might be attractive tools to interfere with PoMiCS of mature OLs in different clinical settings.

Material and methods

Transgenic mice

Mice were housed under specific standardized pathogen-free conditions in the animal facility at Ulm University. Food and water were provided ad libidum, a 12light/12dark cycle was obeyed. IKK2-CA^{PLP−CreERT2} was generated by crossing single transgenic Tg(Plp1-cre/ERT2)1Ueli [82] mice (short PLP-CreERT2) with mice single transgenic for Gt(ROSA)26Sor^{tm4(Ikbkb)Rsky} [83]. The RFP^{PLP−CreERT2} Cre reporter line was established by crossing single transgenic Tg(Plp1-cre/ERT2)1Ueli [82] mice with mice single transgenic for Gt(ROSA)26Sor^tm1Hjf [84]. The RFP^{PLP−CreERT2} model allows a direct in situ monitoring of recombination-dependent RFP expression i.e., cellular deletion efficiency. All mouse lines were bred on a C57BL/6 background. Activation of the genetic construct was conducted at the age of 9–13 weeks by 3 intraperitoneal (i.p.). injections of tamoxifen (2 mg tamoxifen dissolved in sun flower seed oil, Sigma-Aldrich, St. Louis, MO, USA) within 5 days and 14 days of tamoxifen enriched food (400 mg tamoxifen citrate/ 1 kg, Genobios, Laval, France), Fig. S1b. Thereafter animals were observed up until 40 weeks post induction (wpi). Wild-type and single transgenic littermates were used as control and just like the double transgenic animals subjected to tamoxifen administration, both male and female animals were included in the study.

All animal experiments were performed in compliance with the guidelines of the German Animal Protection Act and were approved by the Regierungspräsidium Tübingen.

Behavioural analyses

Neurological Severity Score (NSS)

A 10-point NSS originally established to monitor neurological deficits after traumatic brain injury (TBI) was used to assess neurological deficits [34]. This scoring system consists of 10 tests, including tasks to measure cognitive and motor functions (e.g., beam walk, round-stick balance, exit circle, gait pattern, and exploratory interest in new environment), whereby 1 point is given for failure of the task and 0 points for succeeding. Thus, a maximum NSS of 10 points indicates severe neurological dysfunction, with failure of all tasks. In the present study, the NSS was assessed starting post induction at least every 4 weeks up to 40 wpi.

Rotarod

Motor behavior was analyzed with the ENV-575 M rotarod (Med Associates, St. Albans, VT, USA). After 1 min at 4 rpm for adjustment, the cylinder accelerated within 5 min to 40 rpm. The latency until falling off the accelerating rotarod was recorded. For the trained group, pretraining was assessed in the second week of tamoxifen food (consisting of 3 trials each on 3 consecutive days) and analysis was performed at least every 4 weeks up to 40 wpi. One group was left untreated and firstly admitted to pretraining after 20wpi.

Beam walking test

In this test, the mice had to traverse a narrow beam to escape from a small, elevated platform to a closed dark box, with subtle encouragement by the experimenter. For the trained group, a protocol with 4 training trials per day for 3 consecutive days with a 12 mm square beam (length 100 cm) was used and analysis was done in duplicates on different beam sizes (12 mm square, 5 mm square, 17 mm round, 11 mm round) at least every 4 weeks up to 40 wpi. The untrained group was only admitted to 1 day of trials on the 12 mm square beam 20wpi and analyzed on the different beam sizes thereafter.

Grip strength measurement

Grip strength was measured using a grip strength meter at maximum strength mode (Panlab, Harvard Apparatus); 3 trials were recorded and averaged. Grip strength was recorded at least every other week.

Ladderwalk

The ladder consists of two transparent plates (square: 69.5 × 15 cm) and cylindrical ladder rungs (8 cm long, Ø of 2 mm). The ladder walk has a length of 60 cm with a space of 1 cm between rungs for the regular ladderwalk and spaces varying between 0.5 and 2 cm for the irregular ladderwalk. Each mouse crossed the beam four times consecutively, while recorded with a Samsung NX1000 camera. The recorded videos were analyzed at ¼ of the original speed to count slips.

Primary cell isolation

Primary OLs and microglia from adult mice were isolated using the Adult Brain Dissociation Kit, OctoMACS® with heaters, O4 and Cd11b beads as well as MS columns in a magnetic field (Miltenyi Biotec, Bergisch-Gladbach, Germany) according to the manufacturer’s protocol. In brief, brains were dissected, enzymatically digested, and debris was removed. The obtained cell suspension was labeled with O4 beads, washed, and then added to an MS column. The pellet of purified O4 positive OLs was snap-frozen in liquid nitrogen (N₂) and stored at -80 °C until further analysis. The flowthrough and three washing volumes of the column containing unlabeled cells were combined and labeled again, this time with CD11b beads, washed and purified using an MS column. The pellet of purified CD11b positive microglia was snap-frozen in liquid nitrogen (N₂) and stored at -80 °C until further analysis.

X-Gal staining

Frehsly isolated primary O4 + OLs were seeded as a droplet of 300μL with a concentration of 1 × 10⁶ cells/mL in 0.5%BSA in PBS in the middle of a well in a 6-well plate. After cells were allowed to attach for 1.5 h at room temperature, the well was filled with 0.5%BSA in PBS very carefully before centrifuged at 200 g for 3 min at room temperature. Cells were then stained using the Senescence β-Galactosidase Staining Kit (Cell signaling #9860S) according to manufacturer´s instructions. In brief, cells were fixed for 10 min at room temperature and subsequently covered with staining solution and incubated at 37 °C for 24-48 h. If clear blue color formation was visible, cells were mounted with 70% glycerol. Images were taken with a Leica DM IRB Microscope (Wetzlar, Germany) and analyzed.

Protein extraction and immunoblot analysis

Tissue samples from different brain regions were snap-frozen in liquid nitrogen, pulverized with a pestle, aliquoted, and stored at -80 °C. For protein extraction an aliquot of one spatula was lysed in 200 μL (approximately three times its volume) of KA-lysis buffer (25 mM Tris–HCl, 150 mM NaCl, 25 mM sodium pyrophosphate, 50 mM β-glycerophosphate, 50 mM NaF, 2 mM EGTA, 2 mM EDTA, 1 mM DTT, 10% glycerol, 1% Triton X-100 (pH 8.0)) supplemented with protease inhibitors (1 mM PMSF and CompleteMini Tablet; Roche Diagnostics, Mannheim, Germany). After centrifugation (30 min, 13,000 rpm), the supernatant was used as the total protein extract. Samples were denatured with a fourfold concentrated Laemmli buffer (200 mM Tris–HCl, 15% glycerol, 4% SDS, 5% β-mercaptoethanol, bromphenol blue). Cell pellets were lysed at a concentration of 10⁷ cells per 100 μL SDS sample buffer (62.5 mM Tris–HCl (pH 6.8), 2% SDS, 10% Glycerin, 50 mM DTT, 0.01% bromphenol blue) and sonicated 3 times for 5 s. Protein extracts from tissue samples as well as cell pellets were boiled for 5 min at 100 °C. Equal amounts of protein (50 μg of tissue samples and 25 μL of cell pellet samples) were separated by SDS-PAGE and transferred to nitrocellulose membranes via wet transfer. After blocking with 5% nonfat dry milk in TBS-T (0.05% Tween 20) buffer for 1 h at room temperature, primary antibodies (see below) were incubated in blocking solution overnight at 4 °C or for 2 h at room temperature. After 3 washing steps, membranes were incubated with horseradish peroxidase–coupled secondary antibody for 1 h at room temperature. Membranes were exposed to ECL detection reagent (Thermo Fisher Scientific, Waltham, MA, USA) and developed by ECL.

Histology and immunostaining

Two different techniques were used: a) Brains were fixed by immersion with 4% paraformaldehyde (PFA) overnight at 4 °C, dehydrated, embedded in paraffin, and cut to 7 mm–thick coronal sections on a microtome (Microm HM355S; Thermo Fisher Scientific). For immunofluorescence, after rehydration, heat mediated antigen retrieval was performed with sodium citrate (10 mM, pH 6.0, 0.05% Tween 20) and the tissue sections were additionally incubated with 0.5% Triton X-100 for 30 min. Sections were washed with PBS and blocked with 5% bovine serum albumin (BSA) for 1 h at room temperature. Incubation with primary antibodies (in 5% BSA) was performed overnight at 4 °C and incubated afterward with secondary antibodies (in 5% BSA) for 1 h at room temperature with 100 ng/ml DAPI for nuclear counterstaining. For histology using Luxol Fast Blue (LFB) and Nissl staining, after dehydration sections were incubated in 0.1% LFB solution at 60 °C overnight, rinsed in 95% ethanol and distilled water before incubated in 0.05% Lithium carbonate solution for 5 s followed by two rinses in 70% ethanol for 10 s each. After washing in distilled water, staining was checked under the light microscope and the lithium carbonate incubation with following washing step repeated until a sharp contrast between grey and white matter was visible. Sections were then incubated in 0.1% cresyl violet staining solution for 4 min and thereafter again dehydrated using isopropyl alcohol and xylene and mounted Entellan (Sigma-Aldrich, St. Louis, MO, USA). b) PFA-fixed brain samples were obtained as previously reported [85]. Briefly, the animals were terminally anesthetized with ketamine and xylazine, transcardially perfused with a peristaltic pump (speed: 5 mL/min) by infusing 25 mL of ice-cold PBS followed by 50 mL of cold 4% PFA (pH 7.4). Brain samples were quickly dissected, post-fixed in 4% PFA for 18 h at 4 °C, washed in PBS, and dehydrated in 30% sucrose for 36 h. Samples were sectioned at -18 °C in a cryostat (Leica CM1950, Wetzlar, Germany) at a thickness of 30 μm. The sections were washed in PBS, blocked in PBS + 3% BSA + 0.5% Triton-X or PBS + 10% goatserum + 0.5% Triton-X and incubated with the appropriate antibody combination from 16–72 h at 4 °C, followed by washing in PBS (45 min × 3) and incubation with a corresponding combination of fluorophore-coupled secondary antibodies for 2 h at room temperature; after washing, sections were dried and mounted with Mowiol® (Carl Roth, Karlsruhe, Germany). For each experimental group or timepoint, at least three animals were processed and analyzed. Images were taken with an All-in-one fluorescence microscope BZ-X810 from Keyence (Neu-Isenburg, Germany) with a DAPI, FITC, TexasRed®, Cy5®, and a bright field filter, 10–40 × magnification, the BZ Viewer for capturing, and BZ Analyzer for merging. Images of stained sections were captured with equal exposure times per channel and the same graphical pre-processing within one experiment. For analysis and quantification, the ImageJ software (Rasband, W.S., ImageJ, U. S. National Institutes of Health, Bethesda, Maryland, USA) was used.

Antibodies for immunoblotting and immunostaining

The following antibodies were used for immunoblot analysis: rabbit-anti-IKK2 (CST-2678, Cell Signaling, Danvers, MA, USA), rabbit-anti-p-p65 (CST-3033, Cell Signaling, Danvers, MA, USA), rabbit-anti-p65 (sc-372, Dallas, TX, USA), chicken-anti-GFP (ab13970, Cambridge, UK), rabbit-anti-tubulin (ab6046, Cambridge, UK), rabbit-anti-PLP1 (CST-28702, Cell Signaling, Danvers, MA, USA), rabbit-anti-MOG (CST-96457, Cell Signaling, Danvers, MA, USA), rabbit-anti-MBP (CST-2396, Cell Signaling, Danvers, MA, USA), rabbit-anti-GAPDH (CST-3686, Cell Signaling, Danvers, MA, USA), rabbit-anti-p-eIF2α (CST-3597, Cell Signaling, Danvers, MA, USA), rabbit-anti-eIF2α (CST-5324, Cell Signaling, Danvers, MA, USA), rabbit-anti-ERK2 (sc-1647, Dallas, TX, USA).

For immunofluorescence, the following primary antibodies were used: mouse-anti-CC1 (OP80, Merck, Darmstadt, Germany), mouse-anti-GSTπ (BD610719, BD, Franklin Lakes, NJ, USA), chicken-anti-GFP (GFP-1020, AvesLab, Davis, CA, USA), mouse-anti-GFAP (sc-33673, Dallas, TX, USA), rabbit-anti-NG2 (AB5320, Merck, Darmstadt, Germany), rabbit-anti-MBP (Biolegend 836,504, San Diego, CA, USA), mouse-anti-Neurofilament-H (SMI32P) (Biolegend 801,701, San Diego, CA, USA), rabbit-anti-ß3-tubulin (Biolegend 802,001, San Diego, CA, USA), rabbit- anti RFP (abcam ab124754, Cambridge, UK).

RNA extraction, cDNA synthesis, and qRT-PCR

Total RNA from tissue samples and cell pellets was isolated using the Peq-Gold Trifast Kit (Peqlab, Erlangen, Germany) or RNA-Solv® Reagent (Omega Bio-Tek, Norcross, GA, USA) as described in the manufacturer’s protocol. 1 μg of total RNA was used to synthesize cDNA with the Transcriptor High Fidelity cDNA Synthesis kit (Roche, Grenzach-Whylen, Germany) with oligo-dT-primers according to the manufacturer’s instructions. Quantitative PCR (qPCR) assays were run in the Lightcycler 480 Instrument (Roche, Grenzach-Whylen, Germany) with primers and hydrolysis probes designed by the Roche Universal Probe Library (UPL) system. As a reference gene, the housekeeping gene hypoxanthine–guanine phosphoribosyl transferase (Hprt) was used. Primer sequences and UPLs are available upon request.

Electron microscopy

Brains were quickly dissected and fixed in 0.1% Glutaraldehyde (GA) (Agar Scientific, Stansted, UK), 4% PFA, 2% Sucrose in 0.1 M Sorensen Phosphate Buffer for 24 h. Area of interest (Corpus Callosum) was dissected sagittal and again fixed overnight in 2.5% GA, 0.1% Sucrose in 0.1 M Sorensen Phosphate Buffer. Samples were then washed in PBS and post-fixed in 2% osmium tetroxide in PBS. After dehydrating the samples in a graded series of isopropanol, they were blockstained in 2% uranyl acetate in ethanol and embedded in Epon. Semi-thin (500 nm) sections were stained with toluidine blue and analyzed using light microscopy to find the area of interest. Ultra-thin Sects. (80 nm) were cut on a microtome using a diamond knife (Leica EM UC7, Wetzlar, Germany) and collected on carbon coated formwar films on 200 mesh copper grids (Plano, Wetzlar, Germany) contrasted with 0.3% lead citrate for 1 min and imaged using a TEM 1400 (Jeol, Tokyo, Japan).

Cellular respiration and extracellular acidification

Primary OLs and microglia (see above) were seeded in XF96 V3 PS cell culture Microplates (Agilent Technologies, Santa Clara, USA) coated with 0.01% Poly-D-Lysin (Thermo Fisher Scientific, Waltham, MA, USA) at a density of 60.000 cells/well and 50.000 cells/well, respectively. OLs were plated in the following medium: NeuroMacs Medium supplemented with 2% NeuroBrew-21 (Miltenyi Biotec, Bergisch-Gladbach, Germany), 1% penicillin/streptomycin, 0.5 mM Glutamine (Thermo Fisher Scientific, Waltham, MA, USA). For proliferation, 10 ng/mL PDGF-AA and 10 ng/mL FGF-2 (Peprotech, Hamburg, Germany) were added freshly to the medium. After two days, 2 nM Triiodothyronine (T3) (Sigma-Aldrich, St. Louis, MO, USA) was added for differentiation and furthermore every second day until measurement at day 8. Microglia were seeded in DMEM supplemented with 10% FBS, 1% penicillin/streptomycin, 2 mM Glutamine (Thermo Fisher Scientific, Waltham, MA, USA). The medium was exchanged every second day until measurement at day 8. On the day of measurement, the cells were washed with XF assay medium (Agilent Technologies, Santa Clara, USA) containing 1 mM sodium pyruvate and 2 mM glutamine and incubated in fresh XF medium for 1 h under CO₂ free conditions. Thereafter, oxygen consumption and extracellular acidification rates (OCR and ECAR) were measured simultaneously using a Seahorse XFe96 Flux Analyzer (Agilent Technologies, Santa Clara, CA, USA). The following injections and final concentrations were used: glucose (10 mM), oligomycin (2 μM), carbonylcyanide-p-trifluoromethoxyphenylhydrazone (FCCP, 2 μM) and antimycin A (0.5 μM)/ rotenone (0.5 μM). Data were normalized to JanusGreen as a surrogate for cell number per well.

Rna-sequencing analysis

Total RNA extraction was performed as stated above, library construction and RNA-sequencing were conducted by Novogene (London, UK). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dUTP for directional library. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries were pooled and sequenced on Illumina platforms, according to effective library concentration and data amount. The clustering of the index-coded samples was performed according to the manufacturer’s instructions. After cluster generation, the library preparations were sequenced on an Illumina platform and paired-end reads were generated. Raw data (raw reads) of FASTQ format were firstly processed through fastp. In this step, clean data (clean reads) were obtained by removing reads containing adapter and poly-N sequences and reads with low quality from raw data. At the same time, Q20, Q30 and GC content of the clean data were calculated. All the downstream analyses were based on the clean data with high quality. Reference genome (GRCm38) and gene model annotation files were downloaded from genome website browser (NCBI/UCSC/Ensembl) directly. Paired-end clean reads were aligned to the reference genome using the Spliced Transcripts Alignment to a Reference (STAR) software, which is based on a previously undescribed RNA-seq alignment algorithm that uses sequential maximum mappable seed search in uncompressed suffix arrays followed by seed clustering and stitching procedure. STAR exhibits better alignment precision and sensitivity than other RNA-seq aligners for both experimental and simulated data. FeatureCounts was used to count the read numbers mapped of each gene. And then RPKM of each gene was calculated based on the length of the gene and reads count mapped to this gene. RPKM, Reads Per Kilobase of exon model per Million mapped reads, considers the effect of sequencing depth and gene length for the reads count at the same time, and is currently the most commonly used method for estimating gene expression levels [86].

Ex-vivo magnetic resonance imaging

Magnetic resonance imaging (MRI) was performed on a dedicated ultrahigh field 11.7 T small animal system (BioSpec 117/16, Bruker Biospin, Ettlingen, Germany) equipped with a 9 cm gradient insert (BGA-S9) operating with ParaVision 6.01. All data were acquired using a cryogenically-cooled 1H two-element surface (MRI CryoProbe™, Bruker BioSpec, Ettlingen, Germany) transmit/receive coil. For 3D brain imaging: FLASH with acquisition parameters as follows: TR/TE = 55/7 ms, flip angle FA = 15°, matrix 300 × 224 × 244, Δr = 40 × 40 × 40 µm³). field-of-view FOV = 12 × 9 × 9.85 mm3, and bandwidth = 50 kHz using 18 signal averages without interpolation. TA: 14h40m. Sequence weighting was T1-weighted.

Traumatic brain injury – Closed Head Injury model (CHI)

Twelve-week-old wild type or NF-κB reporter gene mice [46] were subjected to experimental CHI with a standardized weight-drop device [34, 87]. In brief, the animals were anesthetized with ketamine (Pfizer Pharma, Karlsruhe, Germany), with an i.p. dose of 100 mg/kg bodyweight, and 2% xylazine (Bayer Health Care, Monheim, Germany), with an i.p. dose of 16 mg/kg body weight Afterward, the skull was exposed by a longitudinal incision of the skin, and a focal blunt injury was induced in the left hemisphere by dropping a 330 g metal rod on the skull from a height of 2.7 cm. After trauma, the mice received supporting oxygenation with 100% O₂, the wound was sutured, and the animals were placed into a warmed recovery cage with ad libitum access to food and water. Buprenorphine analgesia (Temgesic; Essex Pharma, Munich, Germany) was administered subcutaneously (0.03 mg/kg body weight) immediately after trauma and every 8 h thereafter until 24 h.

Statistical analysis

Statistical analyses were performed with Prism software (GraphPad, San Diego, CA, USA) and are indicated in the specific figure legend. One- or 2-way ANOVA with Bonferroni’s correction was used to compare independent measurements at one or different time points, respectively. For nonparametric analysis Mann–Whitney U was used. All data are shown as mean ± SEM, statistical significance was set at P < 0.05.

Availability of data and materials

All data generated in this study are included in this published article. Further RNA-Sequencing data will be made available to the public prior to publishing. All custom scripts are available from the corresponding authors upon reasonable request.

Abbreviations

8-OHdG:: 8-Hydroxyguanosine
AD:: Alzheimer´s disease
CHI:: Closed head injury
CNS:: Central nervous system
DAMP:: Damage-associated molecular patters
ECAR:: Extracellular acidification rate
eIF2:: Eucaryotic initiation factors 2
FACS:: Fluorescence-activated cell sorting
GEF:: Guanidine exchange factor
GFAP:: Glial fibrillary acidic protein
GFP:: Green fluorescent protein
GSEA:: Gene set enrichment analysis
Iba1:: Ionized calcium-binding adapter molecule 1
IKK:: Inhibitor of nuclear factor kappa-B kinase
IKK2-CA:: Constant active IKK2 transgene
ISR:: Integrated stress response
LFB:: Luxol fast blue
MBP:: Myelin basic protein
MOG:: Myelin oligodendrocyte glycoprotein
MRI:: Magnetic resonance imaging
MS:: Multiple sclerosis
NF-κB:: Nuclear factor ‘kappa-light-chain-enhancer’ of activated B-cells
NSS:: Neurological severity score
OCR:: Oxygen consumption rate
OL:: Oligodendrocyte
OPC:: Oligodendroyte progenitor cells
PLP:: Proteolipid protein
PoMiCS:: Post mitotic cellular senescence
SA:: Senescence associated
SASP:: Senenscence associated secretory phenotype
TBI:: Traumatic brain injury
TUNEL:: TdT-mediated dUTP-biotin nick end labeling
VWMD:: Vanishing white matter disease
WAM:: White matter associated microglia
WMD:: White matter degeneration
wpi:: Weeks post induction

References

Mattson MP, Arumugam TV. Hallmarks of brain aging: adaptive and pathological modification by metabolic states. Cell Metab. 2018;27(6):1176–99.
Article CAS PubMed PubMed Central Google Scholar
Drayer BP. Imaging of the aging brain. Part I Normal findings Radiology. 1988;166(3):785–96.
CAS PubMed Google Scholar
Chapman TW, Hill RA. Myelin plasticity in adulthood and aging. Neurosci Lett. 2020;715:134645.
Tang Y, Nyengaard J, Pakkenberg B, Gundersen H. Age-induced white matter changes in the human brain: a stereological investigation. Neurobiol Aging. 1997;18(6):609–15.
Article CAS PubMed Google Scholar
Liu H, Yang Y, Xia Y, Zhu W, Leak RK, Wei Z, Wang J, Hu X. Aging of cerebral white matter. Ageing Res Rev. 2017;34:64–76.
Article PubMed Google Scholar
Kohama SG, Rosene DL, Sherman LS. Age-related changes in human and non-human primate white matter: from myelination disturbances to cognitive decline. Age. 2012;34(5):1093–110.
Article PubMed Google Scholar
Peters A. The effects of normal aging on myelin and nerve fibers: a review. J Neurocytol. 2002;31(8–9):581–93.
Article PubMed Google Scholar
Tse K-H, Herrup K. DNA damage in the oligodendrocyte lineage and its role in brain aging. Mech Ageing Dev. 2017;161:37–50.
Article CAS PubMed Google Scholar
Sams E. Oligodendrocytes in the Aging Brain. Neuronal Signal. 2021;5:NS20210008.
Article CAS PubMed PubMed Central Google Scholar
Chung HY, Kim DH, Lee EK, Chung KW, Chung S, Lee B, Seo AY, Chung JH, Jung YS, Im E. Redefining chronic inflammation in aging and age-related diseases: proposal of the senoinflammation concept. Aging Dis. 2019;10(2):367.
Article PubMed PubMed Central Google Scholar
Sanada F, Taniyama Y, Muratsu J, Otsu R, Shimizu H, Rakugi H, Morishita R. Source of chronic inflammation in aging. Front Cardiovasc Med. 2018;5:12.
Article PubMed PubMed Central Google Scholar
Tan BL, Norhaizan ME. Chronic Inflammation and Aging (Inflammaging). In: The Role of Antioxidants in Longevity and Age-Related Diseases. Springer; 2021. p. 39–50.
Chapter Google Scholar
Hinz M, Scheidereit C. The IκB kinase complex in NF-κB regulation and beyond. EMBO Rep. 2014;15(1):46–61.
Article CAS PubMed Google Scholar
Kaltschmidt B, Kaltschmidt C. NF-κB in the nervous system. Cold Spring Harb Perspect Biol. 2009;1(3): a001271.
Article PubMed PubMed Central Google Scholar
Zhang Q, Lenardo MJ, Baltimore D. 30 Years of NF-kappaB: a blossoming of relevance to human pathobiology. Cell. 2017;168(1–2):37–57.
Article CAS PubMed PubMed Central Google Scholar
Helenius M, Hänninen M, Lehtinen SK, Salminen A. Aging-induced up-regulation of nuclear binding activities of oxidative stress responsive NF-kB transcription factor in mouse cardiac muscle. J Mol Cell Cardiol. 1996;28(3):487–98.
Article CAS PubMed Google Scholar
Baker RG, Hayden MS, Ghosh S. NF-κB, inflammation, and metabolic disease. Cell Metab. 2011;13(1):11–22.
Article CAS PubMed PubMed Central Google Scholar
Glass CK, Saijo K, Winner B, Marchetto MC, Gage FH. Mechanisms underlying inflammation in neurodegeneration. Cell. 2010;140(6):918–34.
Article CAS PubMed PubMed Central Google Scholar
Adler AS, Sinha S, Kawahara TL, Zhang JY, Segal E, Chang HY. Motif module map reveals enforcement of aging by continual NF-κB activity. Genes Dev. 2007;21(24):3244–57.
Article CAS PubMed PubMed Central Google Scholar
Korhonen P, Helenius M, Salminen A. Age-related changes in the regulation of transcription factor NF-κB in rat brain. Neurosci Lett. 1997;225(1):61–4.
Article CAS PubMed Google Scholar
Jurk D, Wilson C, Passos JF, Oakley F, Correia-Melo C, Greaves L, Saretzki G, Fox C, Lawless C, Anderson R. Chronic inflammation induces telomere dysfunction and accelerates ageing in mice. Nat Commun. 2014;5(1):1–14.
Article Google Scholar
Liu K, Feng T, Liu J, Zhong M, Zhang S. Silencing of the DEK gene induces apoptosis and senescence in CaSki cervical carcinoma cells via the up-regulation of NF-κB p65. Biosci Rep. 2012;32(3):323–32.
Article CAS PubMed Google Scholar
Rovillain E, Mansfield L, Caetano C, Alvarez-Fernandez M, Caballero OL, Medema RH, Hummerich H, Jat PS. Activation of nuclear factor-kappa B signalling promotes cellular senescence. Oncogene. 2011;30(20):2356–66.
Article CAS PubMed PubMed Central Google Scholar
Chien Y, Scuoppo C, Wang X, Fang X, Balgley B, Bolden JE, Premsrirut P, Luo W, Chicas A, Lee CS. Control of the senescence-associated secretory phenotype by NF-κB promotes senescence and enhances chemosensitivity. Genes Dev. 2011;25(20):2125–36.
Article CAS PubMed PubMed Central Google Scholar
Salminen A, Kauppinen A, Kaarniranta K. Emerging role of NF-κB signaling in the induction of senescence-associated secretory phenotype (SASP). Cell Signal. 2012;24(4):835–45.
Article CAS PubMed Google Scholar
Niedernhofer LJ, Garinis GA, Raams A, Lalai AS, Robinson AR, Appeldoorn E, Odijk H, Oostendorp R, Ahmad A, Van Leeuwen W. A new progeroid syndrome reveals that genotoxic stress suppresses the somatotroph axis. Nature. 2006;444(7122):1038–43.
Article CAS PubMed Google Scholar
Tilstra JS, Robinson AR, Wang J, Gregg SQ, Clauson CL, Reay DP, Nasto LA, St Croix CM, Usas A, Vo N. NF-κB inhibition delays DNA damage–induced senescence and aging in mice. J Clin Investig. 2012;122(7):2601–12.
Article CAS PubMed PubMed Central Google Scholar
García Osorio F, López Otín C, Pérez Freije JM. NF-kB in premature aging. Aging. 2012;4(11):726–7.
Article Google Scholar
Osorio F, Soria-Valles C, Santiago-Fernández O, Freije J, López-Otín C. NF-κB signaling as a driver of ageing. Int Rev Cell Mol Biol. 2016;326:133–74.
Article CAS PubMed Google Scholar
Zhang L, Zhao J, Gurkar A, Niedernhofer LJ, Robbins PD. Methods to quantify the NF-κB pathway during senescence. In: Cellular Senescence. Springer; 2019. p. 231–50.
Chapter Google Scholar
Lana D, Ugolini F, Nosi D, Wenk GL, Giovannini MG. The emerging role of the interplay among astrocytes, microglia, and neurons in the hippocampus in health and disease. Front Aging Neurosci. 2021;13:156.
Article Google Scholar
Hughes EG, Kang SH, Fukaya M, Bergles DE. Oligodendrocyte progenitors balance growth with self-repulsion to achieve homeostasis in the adult brain. Nat Neurosci. 2013;16(6):668–76.
Article CAS PubMed PubMed Central Google Scholar
von Streitberg A, Jäkel S, von Bernhardi JE, Straube C, Buggenthin F, Marr C, Dimou L. NG2-glia transiently overcome their homeostatic network and contribute to wound closure after brain injury. Front Cell Dev Biol. 2021;9:762.
Google Scholar
Flierl MA, Stahel PF, Beauchamp KM, Morgan SJ, Smith WR, Shohami E. Mouse closed head injury model induced by a weight-drop device. Nat Protoc. 2009;4(9):1328–37.
Article CAS PubMed Google Scholar
Metz GA, Whishaw IQ. The ladder rung walking task: a scoring system and its practical application. JoVE (J Vis Exp). 2009;28:e1204.
Google Scholar
Lattke M, Reichel SN, Magnutzki A, Abaei A, Rasche V, Walther P, Calado DP, Ferger B, Wirth T, Baumann B. Transient IKK2 activation in astrocytes initiates selective non-cell-autonomous neurodegeneration. Mol Neurodegener. 2017;12(1):16.
Article PubMed PubMed Central Google Scholar
Mckenzie IA, Ohayon D, Li H, de Faria JP, Emery B, Tohyama K, Richardson WD. Motor skill learning requires active central myelination. Science. 2014;346(6207):318–22.
Article CAS PubMed PubMed Central Google Scholar
Susuki K. Myelin: a specialized membrane for cell communication. Nature Education. 2010;3(9):59.
Google Scholar
Kuhn S, Gritti L, Crooks D, Dombrowski Y. Oligodendrocytes in development, myelin generation and beyond. Cells. 2019;8(11):1424.
Article CAS PubMed PubMed Central Google Scholar
Sapieha P, Mallette FA. Cellular senescence in postmitotic cells: beyond growth arrest. Trends Cell Biol. 2018;28(8):595–607.
Article CAS PubMed Google Scholar
Nacarelli T, Sell C. Targeting metabolism in cellular senescence, a role for intervention. Mol Cell Endocrinol. 2017;455:83–92.
Article CAS PubMed Google Scholar
Payea MJ, Anerillas C, Tharakan R, Gorospe M. Translational control during cellular senescence. Mol Cell Biol. 2021;41(2):e00512-00520.
Article CAS PubMed PubMed Central Google Scholar
Costa-Mattioli M, Klann E. Translational control mechanisms in synaptic plasticity and memory. In: The Curated Reference Collection in Neuroscience and Biobehavioral Psychology. Elsevier Science Ltd; 2016. p. 311–28.
Google Scholar
Orihuela R, McPherson CA, Harry GJ. Microglial M1/M2 polarization and metabolic states. Br J Pharmacol. 2016;173(4):649–65.
Article CAS PubMed Google Scholar
Crews FT, Vetreno RP. Mechanisms of neuroimmune gene induction in alcoholism. Psychopharmacology. 2016;233(9):1543–57.
Article CAS PubMed Google Scholar
Tomann P, Paus R, Millar SE, Scheidereit C, Schmidt-Ullrich R. Lhx2 is a direct NF-κB target gene that promotes primary hair follicle placode down-growth. Development. 2016;143(9):1512–22.
CAS PubMed PubMed Central Google Scholar
Bacmeister CM, Barr HJ, McClain CR, Thornton MA, Nettles D, Welle CG, Hughes EG. Motor learning promotes remyelination via new and surviving oligodendrocytes. Nat Neurosci. 2020;23(7):819–31.
Article CAS PubMed PubMed Central Google Scholar
Simon C, Gotz M, Dimou L. Progenitors in the adult cerebral cortex: cell cycle properties and regulation by physiological stimuli and injury. Glia. 2011;59(6):869–81.
Article PubMed Google Scholar
von Bernhardi JE, Dimou L. Oligodendrogenesis is a key process for cognitive performance improvement induced by voluntary physical activity. Glia. 2022;70(6):1052–67.
Article Google Scholar
Kato D, Wake H, Lee PR, Tachibana Y, Ono R, Sugio S, Tsuji Y, Tanaka YH, Tanaka YR, Masamizu Y. Motor learning requires myelination to reduce asynchrony and spontaneity in neural activity. Glia. 2020;68(1):193–210.
Article PubMed Google Scholar
Keren-Shaul H, Spinrad A, Weiner A, Matcovitch-Natan O, Dvir-Szternfeld R, Ulland TK, David E, Baruch K, Lara-Astaiso D, Toth B. A unique microglia type associated with restricting development of Alzheimer’s disease. Cell. 2017;169(7):1276–90. e1217.
Article CAS PubMed Google Scholar
Hammond TR, Dufort C, Dissing-Olesen L, Giera S, Young A, Wysoker A, Walker AJ, Gergits F, Segel M, Nemesh J. Single-cell RNA sequencing of microglia throughout the mouse lifespan and in the injured brain reveals complex cell-state changes. Immunity. 2019;50(1):253–71. e256.
Article CAS PubMed Google Scholar
Huang W, Bai X, Meyer E, Scheller A. Acute brain injuries trigger microglia as an additional source of the proteoglycan NG2. Acta Neuropathol Commun. 2020;8(1):1–15.
Article Google Scholar
Sherafat A, Pfeiffer F, Reiss AM, Wood WM, Nishiyama A. Microglial neuropilin-1 promotes oligodendrocyte expansion during development and remyelination by trans-activating platelet-derived growth factor receptor. Nat Commun. 2021;12(1):1–17.
Article Google Scholar
Lalive PH, Paglinawan R, Biollaz G, Kappos EA, Leone DP, Malipiero U, Relvas JB, Moransard M, Suter T, Fontana A. TGF-β-treated microglia induce oligodendrocyte precursor cell chemotaxis through the HGF-c-Met pathway. Eur J Immunol. 2005;35(3):727–37.
Article CAS PubMed Google Scholar
Takase H, Washida K, Hayakawa K, Arai K, Wang X, Lo EH, Lok J. Oligodendrogenesis after traumatic brain injury. Behav Brain Res. 2018;340:205–11.
Article CAS PubMed Google Scholar
Hughes EG, Orthmann-Murphy JL, Langseth AJ, Bergles DE. Myelin remodeling through experience-dependent oligodendrogenesis in the adult somatosensory cortex. Nat Neurosci. 2018;21(5):696–706.
Article CAS PubMed PubMed Central Google Scholar
Hill RA, Li AM, Grutzendler J. Lifelong cortical myelin plasticity and age-related degeneration in the live mammalian brain. Nat Neurosci. 2018;21(5):683–95.
Article CAS PubMed PubMed Central Google Scholar
Mitew S, Gobius I, Fenlon LR, McDougall SJ, Hawkes D, Xing YL, Bujalka H, Gundlach AL, Richards LJ, Kilpatrick TJ. Pharmacogenetic stimulation of neuronal activity increases myelination in an axon-specific manner. Nat Commun. 2018;9(1):306.
Article PubMed PubMed Central Google Scholar
Sim FJ, Zhao C, Penderis J, Franklin RJ. The age-related decrease in CNS remyelination efficiency is attributable to an impairment of both oligodendrocyte progenitor recruitment and differentiation. J Neurosci. 2002;22(7):2451–9.
Article CAS PubMed PubMed Central Google Scholar
Koutsoudaki PN, Papadopoulos D, Passias P-G, Koutsoudaki P, Gorgoulis VG. Cellular senescence and failure of myelin repair in multiple sclerosis. Mech Ageing Dev. 2020;192:111366.
Article CAS PubMed Google Scholar
Zhang P, Kishimoto Y, Grammatikakis I, Gottimukkala K, Cutler RG, Zhang S, Abdelmohsen K, Bohr VA, Sen JM, Gorospe M. Senolytic therapy alleviates Aβ-associated oligodendrocyte progenitor cell senescence and cognitive deficits in an Alzheimer’s disease model. Nat Neurosci. 2019;22(5):719–28.
Article CAS PubMed PubMed Central Google Scholar
Sikora E, Bielak-Zmijewska A, Dudkowska M, Krzystyniak A, Mosieniak G, Wesierska M, Wlodarczyk J. Cellular senescence in brain aging. Frontiers in Aging Neuroscience. 2021;13:646924.
Article CAS PubMed PubMed Central Google Scholar
Rivellini C, Porrello E, Dina G, Mrakic-Sposta S, Vezzoli A, Bacigaluppi M, Gullotta GS, Chaabane L, Leocani L, Marenna S. JAB1 deletion in oligodendrocytes causes senescence-induced inflammation and neurodegeneration in mice. J Clin Invest. 2022;132(3):e145071.
Raasch J, Zeller N, van Loo G, Merkler D, Mildner A, Erny D, Knobeloch KP, Bethea JR, Waisman A, Knust M, et al. IkappaB kinase 2 determines oligodendrocyte loss by non-cell-autonomous activation of NF-kappaB in the central nervous system. Brain. 2011;134(Pt 4):1184–98.
Article PubMed PubMed Central Google Scholar
Stone S, Jamison S, Yue Y, Durose W, Schmidt-Ullrich R, Lin W. NF-kappaB Activation Protects Oligodendrocytes against Inflammation. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2017;37(38):9332–44.
Article CAS PubMed Google Scholar
Gupta AS, Biswas DD, La Shardai NB, Mockenhaupt K, Marone M, Hoskins A, Siebenlist U, Kordula T. A detrimental role of RelB in mature oligodendrocytes during experimental acute encephalomyelitis. J Neuroinflammation. 2019;16(1):1–13.
Article Google Scholar
Duncan ID, Radcliff AB. Inherited and acquired disorders of myelin: the underlying myelin pathology. Exp Neurol. 2016;283:452–75.
Article CAS PubMed PubMed Central Google Scholar
Cunha MI, Su M, Cantuti-Castelvetri L, Müller SA, Schifferer M, Djannatian M, Alexopoulos I, van der Meer F, Winkler A, van Ham TJ. Pro-inflammatory activation following demyelination is required for myelin clearance and oligodendrogenesis. J Exp Med. 2020;217(5):9.
Article Google Scholar
Lei Z, Yue Y, Stone S, Wu S, Lin W. NF-κB activation accounts for the cytoprotective effects of PERK activation on oligodendrocytes during EAE. J Neurosci. 2020;40(33):6444–56.
Article CAS PubMed PubMed Central Google Scholar
Bartzokis G. Age-related myelin breakdown: a developmental model of cognitive decline and Alzheimer’s disease. Neurobiol Aging. 2004;25(1):5–18.
Article CAS PubMed Google Scholar
Sabbatinelli J, Prattichizzo F, Olivieri F, Procopio AD, Rippo MR, Giuliani A. Where metabolism meets senescence: focus on endothelial cells. Front Physiol. 2019;10:1523.
Article PubMed PubMed Central Google Scholar
Sah E, Krishnamurthy S, Ahmidouch MY, Gillispie GJ, Milligan C, Orr ME. The cellular senescence stress response in post-mitotic brain cells: Cell survival at the expense of tissue degeneration. Life. 2021;11(3):229.
Article CAS PubMed PubMed Central Google Scholar
Al-Mashhadi S, Simpson JE, Heath PR, Dickman M, Forster G, Matthews FE, Brayne C, Ince PG, Wharton SB, Function MRCC, et al. Oxidative glial cell damage associated with white matter lesions in the aging human brain. Brain Pathol. 2015;25(5):565–74.
Article CAS PubMed Google Scholar
Sato-Hashimoto M, Nozu T, Toriba R, Horikoshi A, Akaike M, Kawamoto K, Hirose A, Hayashi Y, Nagai H, Shimizu W. Microglial SIRPα regulates the emergence of CD11c+ microglia and demyelination damage in white matter. Elife. 2019;8:e42025.
Article PubMed PubMed Central Google Scholar
Safaiyan S, Besson-Girard S, Kaya T, Cantuti-Castelvetri L, Liu L, Ji H, Schifferer M, Gouna G, Usifo F, Kannaiyan N. White matter aging drives microglial diversity. Neuron. 2021;109(7):1100–17 e1110.
Article CAS PubMed Google Scholar
Gouna G, Klose C, Bosch-Queralt M, Liu L, Gokce O, Schifferer M, Cantuti-Castelvetri L, Simons M. TREM2-dependent lipid droplet biogenesis in phagocytes is required for remyelination. J Exp Med. 2021;218(10):e20210227.
Article CAS PubMed PubMed Central Google Scholar
Lin W, Bailey SL, Ho H, Harding HP, Ron D, Miller SD, Popko B. The integrated stress response prevents demyelination by protecting oligodendrocytes against immune-mediated damage. J Clin Investig. 2007;117(2):448–56.
Article CAS PubMed PubMed Central Google Scholar
Lin W, Kunkler PE, Harding HP, Ron D, Kraig RP, Popko B. Enhanced integrated stress response promotes myelinating oligodendrocyte survival in response to interferon-γ. Am J Pathol. 2008;173(5):1508–17.
Article CAS PubMed PubMed Central Google Scholar
Chen Y, Kunjamma RB, Weiner M, Chan JR, Popko B. Prolonging the integrated stress response enhances CNS remyelination in an inflammatory environment. Elife. 2021;10:e65469.
Article CAS PubMed PubMed Central Google Scholar
Hanson F, Hodgson RE, de Oliveira MIR, Allen E, Campbell SG. Regulation and function of elF2B in neurological and metabolic disorders. Biosci Rep. 2022;42(6):9.
Article Google Scholar
Leone DP, Genoud St, Atanasoski S, Grausenburger R, Berger P, Metzger D, Macklin WB, Chambon P, Suter U. Tamoxifen-inducible glia-specific Cre mice for somatic mutagenesis in oligodendrocytes and Schwann cells. Mol Cell Neurosci. 2003;22(4):430–40.
Article CAS PubMed Google Scholar
Sasaki Y, Derudder E, Hobeika E, Pelanda R, Reth M, Rajewsky K, Schmidt-Supprian M. Canonical NF-κB activity, dispensable for B cell development, replaces BAFF-receptor signals and promotes B cell proliferation upon activation. Immunity. 2006;24(6):729–39.
Article CAS PubMed Google Scholar
Luche H, Weber O, Nageswara Rao T, Blum C, Fehling HJ. Faithful activation of an extra-bright red fluorescent protein in “knock-in” Cre-reporter mice ideally suited for lineage tracing studies. Eur J Immunol. 2007;37(1):43–53.
Article CAS PubMed Google Scholar
Ouali Alami N, Schurr C, Olde Heuvel F, Tang L, Li Q, Tasdogan A, Kimbara A, Nettekoven M, Ottaviani G, Raposo C, et al. NF-kappaB activation in astrocytes drives a stage-specific beneficial neuroimmunological response in ALS. EMBO J. 2018;37(16):e98697.
Article PubMed PubMed Central Google Scholar
Mortazavi A, Williams BA, McCue K, Schaeffer L, Wold B. Mapping and quantifying mammalian transcriptomes by RNA-Seq. Nat Methods. 2008;5(7):621–8.
Article CAS PubMed Google Scholar
Chen Y, Constantini S, Trembovler V, Weinstock M, Shohami E. An experimental model of closed head injury in mice: pathophysiology, histopathology, and cognitive deficits. J Neurotrauma. 1996;13(10):557–68.
Article CAS PubMed Google Scholar

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Acknowledgements

We thank Petra Weihrich, Melanie Gerstenlauer and Uta Manfras for excellent technical assistance.

Funding

Open Access funding enabled and organized by Projekt DEAL. This research was also funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) project 251293561-SFB 1149—Collaborative Research Center CRC1149 project A03 to T.W. This work was further funded by the DFG project 450627322-SFB 1506, CRC1506 project A05 to F.O. and B.B

Author information

Judith Stefanie Schlett and Melanie Mettang contributed equally to this work.
Thomas Wirth and Bernd Baumann contributed equally to this work as senior authors.

Authors and Affiliations

Institute of Physiological Chemistry, Ulm University, Albert- Einstein-Allee 11, 89081, Ulm, Germany
Judith Stefanie Schlett, Melanie Mettang, Pavel Schweizer, Alina Errerd, Ephraim Alemayehu Mulugeta, Tabea Melissa Hein, Konstantinos Tsesmelis, Miltiadis Tsesmelis, Ulrike F. G. Büttner, Heinrich Wendt, Vivien Prex, Thomas Wirth & Bernd Baumann
Molecular and Translational Neuroscience, Department of Neurology, University Medical Center Ulm, 89081, Ulm, Germany
Aladdin Skaf, Ester Nespoli & Leda Dimou
Core Facility Small Animal Imaging (CF-SANI), Ulm University, 89081, Ulm, Germany
Alireza Abaei & Volker Rasche
Institute of Clinical Neuroanatomy, Ulm University, Helmholtzstraße 8/1, 89081, Ulm, Germany
Najwa Ouali Alami & Deniz Yilmazer-Hanke
Core Facility Extracellular Flux Analyzer, Ulm University Medical Center, 89081, Ulm, Germany
Daniel Tews
Central Facility for Electron Microscopy, Ulm University, 89081, Ulm, Germany
Paul Walther
Department of Internal Medicine I, Center for Internal Medicine, University Medical Center Ulm, 89081, Ulm, Germany
Franz Oswald

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Judith Stefanie Schlett
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Melanie Mettang
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Aladdin Skaf
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Pavel Schweizer
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Alina Errerd
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Ephraim Alemayehu Mulugeta
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Tabea Melissa Hein
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Konstantinos Tsesmelis
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Miltiadis Tsesmelis
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Ulrike F. G. Büttner
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Heinrich Wendt
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Alireza Abaei
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Volker Rasche
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Ester Nespoli
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Najwa Ouali Alami
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Daniel Tews
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Paul Walther
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Deniz Yilmazer-Hanke
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Franz Oswald
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Leda Dimou
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Thomas Wirth
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Bernd Baumann
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Contributions

Conceptualization, B.B. and T.W.; J.S.S., M.M., A.S., P.S., A.E., E.A.M., T.M.H., K.T., M.T., H.W., A.A., U.F.G.B., V.P., E.N., D.T., and P.W. performed experiments and analyzed data; writing—original draft preparation, J.S.S., M.M., T.W. and B.B.; writing—review and editing, J.S.S., M.M., N.Q.A, D.Y.-H., F.O., V.R., L.D., T.W. and B.B.; supervision, T.W. and B.B.; project administration, T.W. and B.B.; funding acquisition, F.O., B.B. and T.W. All authors have read and agreed to the submitted version of the manuscript.

Corresponding author

Correspondence to Bernd Baumann.

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Ethics approval and consent to participate

The study was conducted according to the guidelines of the Declaration of Helsinki. All experiments were performed in compliance with institutional guidelines (Tierforschungszentrum TFZ, Ulm University) and German Animal Protection Act and approved by the Regierungspräsidium Tübingen (Tübingen, Germany).

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All authors have read and approved the manuscript submitted.

Competing interests

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

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Supplementary Information

Additional file 1: Supplementary Figure 1.

Functional characterization of the IKK2-CA^PLP-CreERT2 mouse model. a Scheme of the conditional transgenic IKK2-CA^PLP-CreERT2 mouse model. Expression of a constitutive-active version of IKK2 (IKK2-CA) and the IRES driven eGFP reporter gene is inhibited through a stopper cassette until removed by tamoxifen-activated Cre recombinase. Cre recombinase expression under control of the PLP promotor ensures specificity to mature OLs. b Transgene expression was induced by 3 intraperitoneal injections of tamoxifen (2 mg) followed by tamoxifen-enriched food (400mg/kg) for 14 days. c and d Immunohistochemical analysis of RFP^PLP-CreERT2 mice revealed no leakiness of the transgenic system. Control and uninduced RFP^PLP-CreERT2 animals (no tamoxifen treatment) revealed no RFP expression, while recombination efficiency in RFP^PLP-CreERT2 animals was found at 63.2%±4.8% (n = 3). e Immunoblot analysis of midbrain and cerebellar tissue samples isolated from control and IKK2-CA^PLP-CreERT2 mice at 3 wpi. Transgene activation was demonstrated by prominent IKK2 and eGFP expression, abundance of p-p65 validates functional activation of IKK/NF-κB signaling (n = 3). f Elevated Ccl5 gene expression levels in cortex, CC, cerebellar and spinal cord tissue 3 wpi indicate an inflammatory milieu within the CNS (n = 3-4). g NF-κB target genes iNos, IL1b and Lcn2 are prominently upregulated in the OL-rich corpus callosum whereas expression is unchanged in spinal cord, cortex and cerebellum. h and i Immunohistochemical analysis of Ki67+/Pdgfrα+ double positive cells revealed a significantly increased number of proliferating precursor cells. (3 wpi – Control (n = 3): 8.3±4.3 cells/mm², IKK2-CA^PLP-CreERT2 (n = 3): 30.8±3.2 cells/mm²; 20 wpi – Control (n = 3): 5.0±3.6 cells/mm², IKK2-CA^PLP-CreERT2 (n = 3): 10.1±3.8 cells/mm²). j and k Expression of the IKK2-CA transgene is restricted to mature OLs. Arrows indicate no colocalization of the GFP reporter gene with NG2 staining marking progenitor cells (cortex tissue, 3wpi). (I) Sciatic nerve tissue does not show GFP reporter gene expression in control or IKK2-CA^PLP-CreERT2 animals. As positive control sciatic nerve tissue from a Sox10-GFP reporter mouse was used, which shows robust expression of GFP in Schwann cells. Data are shown as mean ± SEM. Grey bars, control; red bars, IKK2-CA^PLP-CreERT2. Statistical analysis: One-way or Two-way ANOVA multiple comparison test followed by Bonferroni´s post hoc test (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 NS, non-significant (p > 0.05)). Supplementary Figure 2. Consequences of IKK2-CA activation in OLs. a Weight development was observed over the time course of 40 weeks. IKK2-CA^PLP-CreERT2 mice show normal weight gain until 19 wpi, thereafter natural weight gain is decreased in both male and female mice. b IKK2-CA^PLP-CreERT2 mice do not lose pre-learnt motoric skills. If animals were introduced to the rotarod task immediately after transgene activation, performance of IKK2-CA^PLP-CreERT2 and control animals was comparable over the entire time course of 40 weeks. c and d IKK2-CA activation in oligodendrocytes does affect cerebellar homeostasis. Measurement of cerebellar width showed no differences between IKK2-CA^PLP-CreERT2 and control animals. The black line in D indicates the measured length (n = 5-7). e Motoric deficits do not issue from purkinje cell loss. Histochemical analysis using Nissl staining of the cerebellum shows intact morphology and normal purkinje cell number as indicated by black arrows. f Immunohistochemical analysis using SMI32 did not reveal axonal pathology in IKK2CA^PLPCreERT2 animals. As a positive control tissue slides from TBI treated wildtype animals were used. Data are shown as mean ± SEM. Black dots/grey bars, control; red dots/bars, IKK2-CA^PLP-CreERT2. Statistical analysis: Two-way ANOVA multiple comparison test followed by Bonferroni´s post hoc test, Two-tailed Mann-Whitney-U Test (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 NS, non-significant (p > 0.05)). Supplementary Figure 3. NF-ĸB activation in OLs leads to white matter loss in the corpus callosum. a and b Ex-vivo MRI analysis of the brain at 20 wpi reveals reduced signal strength (appearing brighter) in the CC as well as reduction in width of the CC of IKK2-CA^PLP-CreERT2 mice. White arrows indicate the CC. c Quantification of the width of the CC (n = 3-4). d and e Distribution of the axon diameter of myelinated axons in the CC is not changed IKK2-CA^PLP-CreERT2 and control animals at 3 wpi and 20 wpi. f Diameter of unmyelinated axons depicted as single values reveals a greater scattering of the axon diameter of unmyelinated axons in the CC of IKK2-CA^PLP-CreERT2 mice 20 wpi. g – i Luxol Fast Blue (LFB) staining revealed a significant decrease in the width of the CC in IKK2-CA^PLP-CreERT2 animals at different bregma points at 20 wpi. Bregma points evaluated were Bregma -1mm (g), Bregma (h) and Bregma+1mm (i). j Quantification of g – i (Bregma-1mm: 3wpi: Control: 348±21.7 μm, IKK2-CA^PLP-CreERT2: 344±16.8 μm; 20wpi: Control: 442±17.3 μm, IKK2-CA^PLP-CreERT2: 353±16.2 μm; Bregma: 3wpi: Control: 231±8.9 μm, IKK2-CA^PLP-CreERT2: 225±2.3 μm; 20wpi: Control: 235±3.1 μm, IKK2CA^PLP-CreERT2: 184±13.0 μm; Bregma+1mm: 3wpi: Control: 508±40.6 μm, IKK2CA^PLPCreERT2: 498±32.9 μm; 20wpi: Control: 624±3.9 μm, IKK2-CA^PLP-CreERT2: 503±22.5 μm n = 3-4). Data are shown as mean ± SEM. Black lines/grey bars, control; red lines/bars, IKK2-CA^PLP-CreERT2. Statistical analysis: Two-way ANOVA multiple comparison test followed by Bonferroni´s post hoc test, (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 NS, non-significant (p > 0.05)). Supplementary Figure 4. Isolation of O4⁺ OLs from brains of IKK2-CA^PLP-CreERT2 mice and subsequent transcriptome analysis. a Schematic workflow of the consecutive isolation of primary O4⁺ OLs and CD11b⁺ microglia from adult mouse brains. After tissue dissociation, the obtained cell suspension is treated with magnetic beads labeled with O4 antibodies thus allowing separation of O4⁺ OLs in a magnetic field. The remaining cell suspension is again treated with magnetic beads, this time labeled with Cd11b antibodies allowing isolation of Cd11b⁺ microglia in a second step. b Cell number of isolated O4⁺ OLs does not significantly differ between IKK2-CA^PLP-CreERT2 and control animals at 3 and 20 wpi (3 wpi: Control: 0.90±0.05 x10⁶, IKK2-CA^PLP-CreERT2: 0.81±0.05 x10⁶ n = 20-23, 20 wpi: Control: 1.1±0.07 x10⁶, IKK2-CA^PLP-CreERT2: 1.3±0.09 x10⁶, n = 10-23, two brains with identical genotype were pooled and set to n = 1). c and d qRT-PCR reveals strong upregulation of the IKK2 transgene (IKK2-CA) as well as GFP reporter gene in O4⁺ OLs from IKK2-CA^PLP-CreERT2 animals, e western blot analysis indicates robust expression of IKK2-CA protein solely in IKK2-CA^PLP-CreERT2 O4⁺ OLs. Cortex tissue from IKK2-CA^PLP-CreERT2 mice served as positive control (n = 3). f FACS analysis of cells isolated with O4⁺ beads shows a strong enrichment (70%) of O4^high/CD11b^low cells. GFP expressing IKK2-CA^PLP-CreERT2 O4⁺ OLs are strongly enriched within the isolated O4⁺ OL cell population. Quantification of the GFP+ O4⁺ cells 3 and 20 wpi (3 wpi: 27.8±4.1 % n = 9, 20 wpi: 9.2±1.5 % n = 5). g FACS staining of O4⁺ OLs with GalC and MOG as markers for intermediate and mature OLs revealed strong enrichment of myelinating OL within the bead-sorted O4 fraction without differences between control and IKK2-CA^PLP-CreERT2 O4⁺ OLs (GalC: Control: 70.74±1.64 %, IKK2-CA^PLP-CreERT2: 72.73±1.80 %; MOG: Control: 56.14±3.20 %, IKK2-CA^PLP-CreERT2: 58.85±4.04 %, n = 3-5). h and i Volcano plot depicting upregulated and downregulated genes in O4⁺ OLs from IKK2CA^PLP-CreERT2 mice compared to control mice at 3 and 20 wpi, revealed by RNA sequencing analysis. j and k RNA sequencing reveals significantly enriched NF-ĸB target gene expression (i) in IKK2-CA^PLP-CreERT2 O4⁺ OLs whereas genes connected to the GO class myelin sheath formation (GO: 0043209) (j) are only slightly deregulated as 3 and 20 wpi (as indicated in the heatmaps). Out of the genes depicted only Mobp and Plec were found significantly deregulated at 3 and 20 wpi, while at 3 wpi additionally Tppp, Plp1 and Msn reached significance, as well as Tubb4b, Calm1 and Gsn at 20 wpi. Data are shown as mean ± SEM. Grey bars, control; red bars, IKK2-CA^PLP-CreERT2. Statistical analysis: Two-tailed Mann-Whitney-U Test, (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 NS, non-significant (p > 0.05)). Supplementary Figure 5. Gene enrichment analysis of O4+OLs with activated IKK/NFκB signaling. a and b GSEA shows strong enrichment in gene sets of cellular senescence (a) and p53 signaling pathway (b) in IKK2-CA^PLP-CreERT2 O4⁺OLs compared to control O4⁺OLs at 3 wpi. c Heatmap depicts strong significant overall upregulation of SASP factors in IKK2CA^PLPCreERT2 O4⁺OLs compared to control O4⁺OLs both at 3 and 20 wpi. d Schematic overview of translational arrest by phosphorylation of eucaryotic initiation factor 2, subunit alpha (eIF2α). Stress-activated kinases (e.g., PERK, PKR, GCN2 and HRI) are able to phosphorylate eIF2α leading to the inhibition of GDP to GTP exchange resulting in global translational arrest. e RNA sequencing analysis reveals enrichment in proteasome (MMU03050) in IKK2CA^PLPCreERT2 O4⁺OLs 20 wpi. Supplementary Figure 6. NF-ĸB activation in OLs initiates an aging-associated microglia phenotype. a Bead-Isolated Cd11b⁺ microglia were subjected to FACS analysis using Cd11b-PE labeled antibody and revealed a purity of 83%. b and c Volcano plot depicting upregulated and downregulated genes in Cd11b⁺ microglia from IKK2-CA^PLP-CreERT2 mice compared to control mice at 3 and 20 wpi (determined by RNA sequencing analysis). d Cd11b⁺ microglia isolated from IKK2-CA^PLP-CreERT2 animals (3 wpi) show an overlap with aged white matter associated microglia (WAM) in gene sets involved in phagocytic activity and metabolism (Safaiyan 2021). IKK2-CA^PLP-CreERT2 derived Cd11b⁺microglia exhibit a more pronounced elevation of genes related to lipid-metabolism and phagosome activity compared to WAM and activated microglia. Genes linked to ribosomal subunits and mitochondrial genes are rather downregulated in WAM IKK2-CA^PLP-CreERT2 Cd11b⁺microglia. e GSEA uncovers gene signatures of cellular senescence in IKK2-CA^PLP-CreERT2 Cd11b⁺ microglia compared to control microglia at 3wpi.

Additional file 2:

Table S1. Differentially regulated genes associated with stress response, comparison of RNA Seq analysis of O4+OL isolated from IKK2-CAPLP-CreERT2 mice 3 wpi and TBI insult area bulk tissue. Table S2. Differentially regulated genes associated with Cellular Senescence, comparison of RNA Seq analysis of O4+OL isolated from IKK2-CAPLP-CreERT2 mice 3 wpi and TBI insult area bulk tissue. Table S3. Differentially regulated genes encoding for SASP Factors, comparison of RNA Seq analysis of O4+OL isolated from IKK2-CAPLP-CreERT2 mice 3 wpi and TBI insult area bulk tissue. Table S4. Differentially regulated genes associated with Myelin Sheath (GO Term: 0043209), comparison of RNA Seq analysis of O4+OL isolated from IKK2-CAPLP-CreERT2 mice 3 wpi and TBI insult area bulk tissue. Table S5. Differentially regulated genes in microglia associated with NG2-glia/OPC proliferation, RNA Seq analysis of Cd11b+ microglia cells isolated from IKK2-CAPLP-CreERT2 mice 3 and 20 wpi.

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Schlett, J.S., Mettang, M., Skaf, A. et al. NF-κB is a critical mediator of post-mitotic senescence in oligodendrocytes and subsequent white matter loss. Mol Neurodegeneration 18, 24 (2023). https://doi.org/10.1186/s13024-023-00616-5

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Received: 11 October 2022
Accepted: 25 March 2023
Published: 17 April 2023
DOI: https://doi.org/10.1186/s13024-023-00616-5

NF-κB is a critical mediator of post-mitotic senescence in oligodendrocytes and subsequent white matter loss

Abstract

Background

Methods

Results

Conclusions

Graphical Abstract

Background

Results

NF-κB activation in Oligodendrocytes initiates inflammatory conditions in the CNS

IKK2-CAPLP−CreERT2animals develop neurological deficits and decreased motoric learning

IKK/NF-κB activation in oligodendrocytes promotes white matter loss

Myelination and myelin homeostasis associated gene expression is only marginally affected by NF-κB activation in oligodendrocytes

IKK/NF-κB activation in mature oligodendrocytes induces a specific stress and senescence program eliciting translational arrest

IKK/NF-κB activation in oligodendrocytes promotes microglia polarization towards aged white matter microglia

Traumatic brain injury promotes senescence of oligodendrocytes and decline in MBP protein

Discussion

Material and methods

Transgenic mice

Behavioural analyses

Neurological Severity Score (NSS)

Rotarod

Beam walking test

Grip strength measurement

Ladderwalk

Primary cell isolation

X-Gal staining

Protein extraction and immunoblot analysis

Histology and immunostaining

Antibodies for immunoblotting and immunostaining

RNA extraction, cDNA synthesis, and qRT-PCR

Electron microscopy

Cellular respiration and extracellular acidification

Rna-sequencing analysis

Ex-vivo magnetic resonance imaging

Traumatic brain injury – Closed Head Injury model (CHI)

Statistical analysis

Availability of data and materials

Abbreviations

References

Acknowledgements

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Competing interests

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Supplementary Information

Additional file 1: Supplementary Figure 1.

Additional file 2:

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Keywords

Molecular Neurodegeneration

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IKK2-CA^{PLP−CreERT2}animals develop neurological deficits and decreased motoric learning