- Open Access
HDAC6 as a target for neurodegenerative diseases: what makes it different from the other HDACs?
Molecular Neurodegeneration volume 8, Article number: 7 (2013)
Histone deacetylase (HDAC) inhibitors have been demonstrated to be beneficial in animal models of neurodegenerative diseases. Such results were mainly associated with the epigenetic modulation caused by HDACs, especially those from class I, via chromatin deacetylation. However, other mechanisms may contribute to the neuroprotective effect of HDAC inhibitors, since each HDAC may present distinct specific functions within the neurodegenerative cascades. Such an example is HDAC6 for which the role in neurodegeneration has been partially elucidated so far. The strategy to be adopted in promising therapeutics targeting HDAC6 is still controversial. Specific inhibitors exert neuroprotection by increasing the acetylation levels of α-tubulin with subsequent improvement of the axonal transport, which is usually impaired in neurodegenerative disorders. On the other hand, an induction of HDAC6 would theoretically contribute to the degradation of protein aggregates which characterize various neurodegenerative disorders, including Alzheimer’s, Parkinson’s and Hutington’s diseases. This review describes the specific role of HDAC6 compared to the other HDACs in the context of neurodegeneration, by collecting in silico, in vitro and in vivo results regarding the inhibition and/or knockdown of HDAC6 and other HDACs. Moreover, structure, function, subcellular localization, as well as the level of HDAC6 expression within brain regions are reviewed and compared to the other HDAC isoforms. In various neurodegenerative diseases, the mechanisms underlying HDAC6 interaction with other proteins seem to be a promising approach in understanding the modulation of HDAC6 activity.
Histone deacetylases (HDACs) are enzymes that deacetylate lysine residues from histones as well as from several other nuclear, cytoplasmic and mitochondrial non-histone proteins. In mammals, 18 HDACs have been phylogenetically classified into four classes. Classes I, II, and IV belong to the Rpd3/Hda1 family . Class I includes the constitutively expressed HDACs 1 to 3 and HDAC8 . Class II is subdivided into classes IIa (HDAC4, 5, 7, and 9) and IIb (HDAC6 and 10). Enzymes from class IIa are able to shuttle between the cytosol and the nucleus, and show a weaker deacetylase activity . Class IIb is mostly found in the cytosol with a preference for non-histone proteins , whereas HDAC11 is the sole member of class IV. These HDACs are usually referred as classical HDACs, whereas class III, called sirtuins, are NAD+ dependent enzymes with different structural features .
The role of HDACs has been studied within several cell processes based on phenotypic changes after isoform-specific knockdown or treatment with HDAC inhibitors. The consequences of an inhibition of HDACs may result in contradictory results, which seem to depend partially on cell type . Knockout analyses of various class I and class II HDAC proteins suggested that class I HDACs are involved in cell proliferation and survival and are expressed ubiquitously in different body tissues, while class II HDACs seem to have tissue-specific roles [7, 8]. Moreover, the specific role of each HDAC is directly related to their specific molecular substrates. To our knowledge, more than 50 non-histone proteins have been identified as substrates for HDACs . On the basis of animal tissue expression and serial analysis of gene expression (SAGE) data from the human transcriptome map [7, 10, 11], the distribution of HDAC isoforms in body tissues is presented in Figure 1 together with their distribution in rat brain . It is also important to notice that the level of expression may differ when specific pathologies are present, such as cancer, where some HDAC isoforms are overexpressed .
The deacetylase activity of HDACs is opposed to that of histone acetyl transferases (HATs) and several studies have demonstrated the relevance of the HDAC/HAT enzymatic balance in neuronal homeostasis . This balance is involved in neurophysiological functions, memory processes and learning. A deregulation of HDAC/HAT activity has been observed in several neurodegenerative diseases (NDs), and a decrease in histone acetylation levels may affect the expression of genes involved in apoptosis and neuroprotection [14–16].
Several reviews discussed the importance of various HDACs in specific NDs [17, 18] and recently, HDAC6 was suggested to be a promising target for some of them . In the present work, we aim at reviewing the published data regarding HDAC6. The structural and functional features of this specific isoform are compared to other classical HDACs. The specific role of HDAC6 in NDs is discussed and the impact of HDAC6 modulation via inhibition, induction and interaction with other proteins in various diseases such as Alzheimer’s (AD), Parkinson’s (PD) and Huntington’s (HD), frontotemporal dementia (FTLD), amyotrophic lateral sclerosis (ALS) and Charcot-Marie-Tooth disease (CMT) is discussed.
Structural differences between HDACs: what makes HDAC6 different from the others
The bacterial HDAC homologue HDLP from Aquifex aeolicus was the first HDAC-like protein structure solved by X-ray in 1999 . Alignment studies combined with structural analyses revealed the presence of a conserved 11 Å deep channel among all HDAC structures, with a zinc ion located at the bottom [21–23]. The zinc-dependent catalytic action consists in the removal of acetyl groups from lysine residues belonging to histone or non-histone proteins . Even if a certain degree of homology in the catalytic domains was found, the so-called zinc-dependent HDACs have been classified into three families (classes I, II and IV) depending on their primary sequence similarity to homologous enzymes from Saccharomyces cerevisiae.
Class I is characterized by four ubiquitous and relatively small enzymes (∼500 amino acids) essentially located in the nucleus of cells [25, 26]. HDACs 1 to 3 are found in complexes with specific transcriptional co-repressors, blocking the expression of tumor suppressor genes . Interestingly, these enzymes share an internal dynamic cavity adjacent to the catalytic pocket that seems to facilitate the egress of the enzymatic products from the active site [20, 22]. Another nuclear zinc-dependent HDAC, HDAC11, was found to be closely related to class I. However, this enzyme did not show enough identity to this class to be placed in it and a new class (IV) was proposed with HDAC11 as the only member .
Class II consists of six larger enzymes (∼1000 amino acids) that can be further classified according to their sequence homology and domain organization into classes IIa and IIb . The N-terminal domain found in class IIa members is the one responsible for nuclear-cytoplasmic shuttling through a phosphorylation-dependent binding to specific 14-3-3 proteins. Such interactions regulate the activity of transcription factors such as the myocyte enhancer factor-2 (MEF2), which exerts a repressor role in a variety of biological functions, from myogenesis to Epstein-Barr virus transcriptional regulation . Moreover, this class shows another zinc ion coordinated to a Cys-Cys-His-Cys motif close to the cavity that may participate in substrate recognition or in protein interactions .
The presence of two catalytic domains in HDAC6 allows this isoform to be classified into class IIb together with HDAC10. While HDAC10 has catalytically inactive domains whose biological function is still unknown, HDAC6 was shown to take part in the microtubule network by acting as a specific α-tubulin deacetylase. Moreover, HDAC6 was able to deacetylate other substrates and to bind ubiquitin, thus modulating cell protective response to cytotoxic accumulation of misfolded and aggregated proteins [19, 29, 30].
To be fully understood, the biological role of HDAC6 requires a deep structural knowledge. The 1215 amino acid residues characterizing the human HDAC6 are arranged in the space to form two independent catalytic domains with a zinc finger ubiquitin-binding domain located at the C-terminus . In the enzymatic structure, it is also important to highlight the presence of a zone characterized by a Ser-Glu containing a tetradecapeptide repeating domain (SE14) responsible for HDAC6 intracellular retention and tau interaction. There are as well two leucine-rich nuclear export sequences (NES1, NES2), which play an essential role in the cytoplasmic/nuclear shuttling process [31, 32] (Figure 2). What makes HDAC6 unique among all HDAC enzymes is the presence of the C-terminal zinc finger domain able to recognize unanchored C-terminal diglycine motif of ubiquitin characterizing aggresomes [29, 33]. This domain, recently solved by X-ray, alone and in complex with ubiquitin, is formed by a compact structure of 5 anti-parallel β-strands, 2 α-helices, and 3 zinc ions with a distinct aromatic pocket . Such a three-dimensional organization is similar to other human zinc finger domains recognizing ubiquitin [34, 35]. Ubiquitin interacts with HDAC6 mainly through a hydrogen bond network. The last three residues of ubiquitin are found in an extended conformation which is stabilized by interactions with the HDAC6 aromatic pocket. Arg 1155 and Tyr 1156 residues act as gatekeepers, moving the ubiquitin binding site from an open to a closed conformation . While the mechanism of aggregate recruitment by HDAC6 via ubiquitin is known from a biological  and structural  point of view, crystallographic information about the two catalytic domains is still missing. The lack of such information is quite problematic for the conception of isoform selective compounds able to modulate HDAC6 activity. Nowadays, this issue is overcome through the generation and refinement of three-dimensional HDAC6 homology models combined with computational interaction and molecular dynamics calculations. Whereas the design of HDAC6 inducers has never been the object of scientific studies, recent reviews accurately describe the structural features that may be interesting for the design of selective HDAC6 inhibitors [22, 26, 36].
Computational and in vitro results were put together to investigate the structural origin of selectivity of the HDAC6 specific inhibitor tubacin . In particular, docking and molecular dynamics calculations highlighted differences in the shape of HDAC surfaces surrounding the binding site. Moreover, the relatively high flexibility of the HDAC6 pocket allowed protein conformational changes by accommodating the cap portion of the studied ligands. These findings were also confirmed by Charrier et al.  and by Kozikowski et al. , through in vitro and docking studies of a set of phenylisoxazole-containing hydroxamates showing IC50 values as low as 2 pM for HDAC6. In both studies, the design and modeling of specific inhibitors were based on the differences found in the region adjacent to the HDAC6 catalytic channel, the so-called cap domain. The HDAC6 homology model built by Butler et al.  revealed that, while the active site is highly conserved among HDACs, the cap domain differs greatly in terms of shape and properties. Moreover, the rim of the catalytic channel appears wider and shallower in HDAC6 compared to the other HDAC channels. Thus, compounds with bulkier and shorter aromatic moieties were designed. For instance, HDAC6 selectivity was enhanced by adding a large and rigid cap group, such as in tubastatin A . Accordingly, in vitro and docking studies on homology models confirmed the HDAC6 selectivity of a series of pyridylalanine-containing hydroxamic acid derivatives . Recently, Kong et al.  developed a fluorescent HDAC6 inhibitor characterized by a planar ring of a dansyl moiety interacting with the hydrophobic portion of the HDAC6 cap domain. The molecule also showed an HDAC4 trapping action by sequestrating this specific nuclear isoform in the cytoplasm, which led to a modification of its expression and function.
Another structural selective element of the HDAC6 catalytic site was identified by studying new HDAC6 hydroxamate inhibitors isolated from a virtual screening of 55,000 molecules . The selectivity of these compounds was explained by the presence of a small sub-pocket close to the zinc ion, able to stabilize the position of the thiazole and pyridine rings characterizing such compounds. Moreover, the carbamated form of one of them was shown to act as a prodrug in cell cultures .
Table 1 summarizes the key amino acid residues in the second catalytic domain of HDAC6, which are responsible for recognition and binding of inhibitors and ubiquitin [44, 45]. The chemical structures of the main HDAC6 specific inhibitors are depicted in Additional file 1.
HDACs other than HDAC6 act mainly as epigenetic modulators in cognition and neuronal death
Several studies show the implication of HDACs, especially those of class I, in memory processes in mice [9, 46]. These processes seem to rely at least in part on epigenetic modulation through HDAC activity on histones, which is demonstrated by the relationship established between cognition, HDAC inhibition and histone acetylation levels . As a matter of fact, HDAC pan-inhibitors were shown to significantly improve long-term memory and learning after inducing neuronal loss in mice. These findings could be correlated with the increase of the acetylation of histones H3 and H4 in the hippocampus and the cortex of mice few hours after being treated [47, 48]. Other studies corroborate the influence of histone acetylation levels in memory. One of them showed that the specific modification of acetylation in histone H4 lysine 12 (H4K12) was able to modify the expression of several genes from the hippocampus involved in memory consolidation. The use of the HDAC inhibitor vorinostat (SAHA) was shown to promote the expression of these genes by increasing the acetylation of H4K12, thus resulting in improved cognitive function in mice . Other pan-inhibitors were extensively studied for their effects on in vitro and in vivo neurodegenerative models. These results, together with the IC50 for various HDAC isoforms, are summarized in supplementary material for SAHA (Additional file 2), scriptaid (Additional file 3), trichostatin A (TSA, Additional file 4), sodium butyrate (Additional file 5) and valproic acid (VA, Additional file 6).
The specific roles of the HDAC isoforms have not been completely elucidated so far. The identification of the isoforms playing a major role in memory and neurodegenerative processes would be rather useful in finding specific inhibitors with a therapeutic potential. Up to now, four HDAC isoforms seem to be closely involved in memory processes: HDACs 1 to 4 [5, 8, 50–60].
Kim et al.  showed that HDAC1 was inactivated in CK-p25 mice. These animals are used in models of NDs, since they overexpress protein p25, which has been associated with some AD features: it accumulates in neurons of AD patients and complexes with Cdk5 kinase. This complex is considered responsible for tau hyperphosphorylation and then for cytoskeletal disruption. By inhibiting HDAC1, p25 may lead to important DNA damage and aberrant cell cycle activity, which would contribute to neuronal death . Interestingly, the overexpression of HDAC1, but not HDAC2, in cells and in an in vivo model of stroke was able to protect neurons from p25 toxicity . Even if the epigenetic role of nuclear HDAC1 has mostly been associated with neuroprotection, cytosolic HDAC1 was found in damaged axons in the brain of humans suffering from multiple sclerosis, in mouse brain after induced demyelination, as well as in cultured neurons exposed to glutamate and TNF-α . It was then demonstrated that the export of HDAC1 from the nucleus to the cytoplasm was induced by pathological conditions and was essential for the onset of axonal damage. The interaction with the nuclear receptor CRM-1 triggered the nuclear export of HDAC1 that formed complexes with proteins of the kinesine family, finally impairing mitochondrial transport. These events were prevented by the treatment with leuptomycin B, an inhibitor of HDAC1 nuclear export, by non-specific HDAC inhibitors and also by silencing HDAC1 but no other HDACs .
The role of HDAC1 and 2 was investigated in primary dissociated hippocampal neurons isolated from floxed HDAC1-, floxed HDAC2-, and floxed HDAC1&2-mice. The deletion of both HDAC1 and 2 during early synaptic development caused a facilitation of excitatory synapse maturation and a modest increase in synapse numbers, which were not observed in the specific deletion of HDAC1 or 2 alone. In contrast, in mature neurons, a decrease of HDAC2 levels alone attenuated basal excitatory transmission without changing the number of detectable nerve terminals. Accordingly, HDAC2-overexpressing mature neurons increased excitatory synapses, suggesting a role for HDAC2 in spontaneous excitatory neurotransmission at least in mature neurons .
While the observation of mature hippocampal neurons from mice overexpressing HDAC2 showed an increase in excitatory synapses , HDAC2 negatively regulated memory processes and synaptic plasticity after neuron-specific overexpression of HDAC2 in transgenic mice . Moreover, the treatment of these HDAC2-overexpressing mice with the non-specific HDAC inhibitor SAHA resulted in improved memory and increased synapse formation .
HDAC3 seems to negatively influence long-term memory processes according to two main observations: first, genetically modified mice with homozygous deletions of Hdac3 showed improved long-term memory; secondly, the administration of the specific HDAC3 inhibitor RGFP136 also provided memory improvement . In addition to this, the overexpression of HDAC3 resulted in cytotoxicity in cortical neurons and in hippocampally derived HT22 cells, but not in primary kidney fibroblasts, HEK293 and HeLa cell lines .
Based on all these published results on nuclear enzymes HDACs 1 to 3, it seems clear that class I HDACs interact with cognitive processes mainly through epigenetic modulation. All of these mechanisms are probably involved together in the enhancement of long-term memory observed in vivo after treatment with an HDAC pan-inhibitor (TSA) and a class I specific inhibitor (MS275) .
Finally, HDAC4 was also taken into consideration by several authors as a promising target to fight against neurodegeneration. This enzyme, as others from the same class, can shuttle between the nucleus and the cytoplasm [5, 8, 56]. It was suggested that HDAC4 is a key effector in the multi-step pathway regulating neuronal death. This hypothesis was supported by two major observations: 1) trafficking of HDAC4 from the cytoplasm into the nucleus induced apoptosis in neurons; 2) the inactivation of HDAC4 resulted in protection from neuronal death . In terms of cognition, the intracellular trafficking of HDAC4 was also related to long-term memory in a Caenorhabditis elegans model. In this worm, the deletion of hda4, a homolog of hdac4, resulted in enhanced learning and long-term memory. Moreover, the expression of the mammalian HDAC4 in the neuronal nuclei of the worm was able to impair neuronal function, but not in the cytosol, suggesting that HDAC4 could impair memory formation through inhibition of gene transcription .
The specific role of HDAC6 in the neurodegenerative cascades
There is enough evidence about the involvement of HDAC6 in several NDs and many inferences could be addressed on the basis of results obtained with specific HDAC6 inhibitors. One of the most studied HDAC6 specific inhibitor is tubacin. IC50, in silico information and biological activities related to NDs are summarized in Table 2 for tubacin together with other known HDAC6 specific inhibitors.
The role of HDAC6 as a tubuline deacetylase was investigated within the cascades of various NDs. It is now clear that HDAC6 plays a central role in protein aggregate elimination, in neuronal oxidative stress and in the mitochondrial transport. The implication of HDAC6 in these three particular processes is discussed below.
HDAC6 and protein aggregates
Protein folding inside cells is not always a spontaneous event. Many synthesized proteins need a complex biological machinery in the cell to achieve their efficient conformation. Large proteins may refold inefficiently, resulting in misfolded intermediates that tend to aggregate . Protein aggregates are usually deleterious to the cell and are a common feature in NDs. HDAC6 is involved within 3 cellular mechanisms able to countervail the accumulation of protein aggregates: 1) the binding to ubiquitinated misfolded proteins, 2) the formation of an aggresome followed by autophagy, and 3) the induction of heat shock proteins (HSPs) (Figure 3). Such mechanisms are involved in the cell response to cytotoxic protein aggregate formation in various NDs [73–75]. The process of autophagy is especially important to counter-balance the accumulation of aggregates since the main route of protein degradation via the ubiquitin-proteasome system is impaired in NDs. Ubiquitin acts as a molecular marker by addressing the substrates to the proteasome 26S in order to be eliminated [76, 77]. The accumulation of protein aggregates in NDs may inhibit proteasome activity . This results in an increased number of highly ubiquitinated misfolded proteins commonly observed in AD, PD and HD [78–87]. At this level, HDAC6 was able to increase cell viability under misfolded protein stress . The enzyme could bind polyubiquitinated proteins thanks to its zinc-finger containing domain . This binding led to the active transport of highly ubiquitinated protein aggregates dispersed within the cytoplasm to constitute a novel organelle, the aggresome, where they were eventually eliminated by autophagy . Moreover, HDAC6 interacted with p97/VCP, an AAATPase (ATPase associated with a variety of activities) which is directly involved in protein degradation via the ubiquitin-proteasome system [4, 90, 91]. As a matter of fact, p97/VCP was able to dissociate the complexes formed between HDAC6 and polyubiquitinated proteins to favor protein degradation. The fate of the ubiquitinated proteins, whether they were being degradated or accumulated into the aggresome depended, at least in part, on the balance between HDAC6 and p97/VCP [92, 93].
HDAC6 could also interact with FAT10, a ubiquitin-like modifier which is another marker for protein elimination. In case of ubiquitin-proteasome impairement, HDAC6 promoted FAT10-containing aggresome in order to eliminate marked protein aggregates . The binding of HDAC6 to polyubiquitinated proteins was also shown to trigger the dissociation of the HDAC6/HSP90/HSF1 complex. This led to the activation of heat shock transcription factor 1 (HSF1) which then induced gene expression of chaperones HSP70 and HSP27, exerting a protective role against the toxic effects of the aggregates in cells . On the other hand, HSP90 together with other proteins are involved in tau burden. The loss or inhibition of HDAC6 correlated with a decrease of tau burden in cells . This may be due to increased HSP90 acetylation, which favors degradation of HSP90 client proteins, such as tau. Chaperones, mainly HSP40 and HSP70, also prevented misfolded protein formation .
HDAC6 and oxidative stress
HDAC6 is involved in the deacetylation of peroxiredoxin-1 and peroxiredoxin-2, two enzymes allowing the reduction of peroxides. Since their acetylation increased their reducing properties, HDAC6 inhibition by tubacin could enhance antioxidant properties in the cell [67, 96], a mechanism to be considered in the case of neurodegeneration. Moreover this inhibition was able to promote neurite growth on myelin-associated glycoprotein and chondroitin sulfate proteoglycan substrates. TSA, scriptaid and sodium butyrate showed the same effects than tubacin, but their reduced selectivity induced some toxicity . Butler et al.  designed a new HDAC6 selective inhibitor, tubastatin A, which showed a neuroprotective effect against oxidative stress induced by homocystic acid. It is also important that no neurotoxicity was observed at the tested concentrations, which is not the case for TSA or other HDAC pan-inhibitors [69, 98]. Thus, selectivity seems to be important to avoid some toxicity .
HDAC6 and the mitochondrial transport
An interesting relationship between HDAC6 and mitochondrial transport was established in hippocampal neurons. Microtubule-dependent intracellular trafficking was shown to be regulated by the activity of HDAC6, via the Akt-GSK3-β signaling pathway . As a matter of fact, the inhibition of GSK3-β increased microtubules acetylation and improved at the same time the mitochondrial transport . However, GSK3-β remains a controversial target in NDs since this protein kinase is involved in many biological processes . GSK3-β is present in large quantities in the brain and HDAC6 is one of its targets . The treatment of hippocampal neurons with tubacin, a specific HDAC6 inhibitor, strongly enhanced mitochondrial movement. This inhibition resulted in higher levels of acetylated tubulin and enhanced binding of the motor protein kinesin-1 to tubulin, which promoted the transport of cargo proteins along microtubules .
The role of HDAC6 in Alzheimer’s disease
The main areas of the brain affected by AD are the hippocampus, entorhinal cortex, associative cortex, and amygdala . Neurofibrillary tangles, a typical intraneuronal lesion in AD, follows a defined progression. Gradually, brain regions affected by the accumulation of tau protein expand, starting at the entorhinal region, an adjacent area of the hippocampus. Then, tau pathology reaches the hippocampus, which plays a major role in memory formation (memory training) and more specifically in the memory of events, called declarative or explicit memory [101, 102]. The expression of HDAC6 in the hippocampus is thus worth of notice. HDAC6 protein level was increased by 52% in AD cortex and by 91% in AD hippocampus, when compared with young normal brains. In order to confirm that HDAC6 protein is overexpressed in AD, the HDAC6 protein level in the brain of patients with AD was compared to age-matched normal brains.
Proteasome inhibition is a well documented feature in AD and seems to potentiate HDAC6-tau interaction. Such interaction could be observed in vitro, in cells and in brains of AD patients. HDAC6 and tau co-localised within the perinuclear aggresome-like compartment, independently of the tubulin deacetylase activity of HDAC6. Treatment with tubacin or HDAC6 knockdown in vivo did not impair the interaction between HDAC6 and tau but was able to decrease tau phosphorylation . Moreover, the post-mortem study of the brain of AD patients denoted low levels of α-tubulin and increased levels of tubulin acetylation. These events were mainly observed in neurons presenting neurofibrillary tangles. By binding to HDAC6, tau inhibited the deacetylase activity and led to an increase in tubulin acetylation. This increase was also observed in human cells over-expressing tau protein. The excess of tau acted as an HDAC6 inhibitor and prevented the autophagy induction followed by proteasome inhibition in cells. Thus, tau can act as an inhibitor of both the deacetylase activity of HDAC6 and the aggresome pathway, depending upon the HDAC6 binding to polyubiquitinated proteins . Ding et al.  hypothesized that even if HDAC6 up-regulation in AD brains contributes to the sequestration of ubiquitinated protein aggregates and recruitment of autophagic components, it would eventually be deleterious to cell survival in AD, due to decreased tubulin acetylation and increased tau phosphorylation. Finally, the positive impact of HDAC6 depletion in cognition has been recently demonstrated by crossing HDAC6 knockdown mice with a model for severe amyloid pathology. The loss of HDAC6 improved the impairment of associative and spatial memory formation and was able to recover the deficits in mitochondrial trafficking induced by Aβ . These findings suggest HDAC6 inhibition as a promising target in AD.
The role of HDAC6 in Parkinson’s disease
PD affects the extrapyramidal system and is characterized by a progressive degeneration of the nigrostriatal dopaminergic (DA) pathway with the presence of Lewy bodies (LBs), which are cytoplasmic inclusions mainly composed of α-synuclein. During the disease, α-synuclein accumulates in an insoluble form within the substantia nigra pars compacta (where HDAC6 is expressed, Figure 1) and other regions . HDAC6 is an attractive target in this process, since this isoform was involved in the elimination of protein aggregates of α-synuclein within a cellular model of PD . Most of the LBs are located in the nigrostriatal DA system but other systems may be affected, including the locus coeruleus (where HDAC6 is also well expressed, Figure 1) and the nucleus basalis of Meynert . HDAC6 was also found in the cortex (Figure 1), another region affected in PD by the presence of LBs and neurofibrillary tangles.
There are some uncertainties on the specific role of LBs in PD [107, 108]. While several authors claimed the in vitro cytotoxicity of α-synuclein in DA neurons [84, 85, 105, 109], Du et al.  demonstrated in the Drosophila model of PD that LBs-like inclusions were rather cytoprotective in the process of PD neurodegeneration. Here, the crucial role of HDAC6 is highlighted. HDAC6 promoted the formation of inclusions from α-synuclein toxic oligomers. The amount of inclusions was increased when HDAC6 was coexpressed with α-synuclein in DA neurons, compared to neurons expressing only α-synuclein. Thus, HDAC6 exerted a cytoprotective role in DA neurons by allowing inclusion formation and decreasing the amount of α-synuclein oligomers in the fly PD model . Furthermore, HDAC6 co-localized with α-synulcein in the perinuclear region to form aggresome-like bodies in a MPP+ induced cellular model of PD. In this specific case, HDAC6 knockdown or inhibition by tubacin resulted not only in the reduction of perinuclear inclusions but also in the increase of α-synuclein in the nucleus with increased cell death. The nuclear accumulation of α-synuclein after HDAC6 inhibition may be strongly related to the role of HDAC6 in the autophagic process of misfolded proteins as an elimination route, an alternative to the ubiquitin-proteasome system impaired in PD and other NDs . A mutation in the gene encoding DJ-1 resulted in misfolding and accumulation of this protein, which is characteristic of an early-onset form of PD. Misfolded DJ-1 aggregates were eliminated by autophagy via parkin-HDAC6 binding . Parkin is an E3 ligase that participates in the addition of ubiquitin molecules in order to mark misfolded proteins. A mutation in the gene encoding parkin is also responsible for an autosomal-recessive form of early PD . In case of proteasome impairment, parkin formed a complex with the heterodimeric E2 enzyme UbcH13/Uev1a to polyubiquitinate misfolded DJ-1 . Even if the mechanisms by which parkin suppressed parkinsonism remain unclear, parkin participated in the elimination of impaired mitochondria (mitophagy) via the HDAC6-dependent mitochondria ubiquitination . Moreover, the HDAC6 inhibitor tubacin prevented the recruitment of parkin by the centrosome via proteasome inhibition .
The role of HDAC6 in Huntington’s disease
HD is caused by gene modification of CAG trinucleotide repeats expansion, resulting in pathological polyglutamine expansion in proteins, and leading to the accumulation of huntingtin aggregates [86, 113–115]. The toxicity of these aggregates in a Drosophila model correlated with changes in histone acetylation, and transcriptional failing . In a transgenic mouse model of HD, the expression of many genes was considerably modified in the striatum. Some components of the ubiquitin-proteasome had their expression increased. This is interesting in the context of this disease, since the ubiquitin-proteasome is directly related to the accumulation of huntingtin .
In a Drosophila model of HD, the co-expression of human chaperones HSP70 and HSP40 acted synergistically to suppress the degenerative phenotype without changing the morphology of cell huntingtin aggregates. In addition, HSP70 and HSP40 were sequestered within the cell aggregates of huntingtin. This might significantly reduce the intracellular availability of these chaperones and lead to an increase in abnormal folding of proteins . Thus, the presence of HDAC6 in these structures may have a protective role in HD through its capacity to induce protective chaperones (cf. chapter 4) .
HDAC6 was shown to be expressed in both structures of the striatum, caudate nucleus and putamen (Figure 1), where the most outstanding neuropathological process of HD takes place.
It has been suggested that HDAC6 forms a complex with both dynein and aggregates (via ubiquitin binding), linking protein aggregates to the microtubule motor necessary for the autophagic process. HDAC6 deacetylase activity was shown to be important for this whole process in HD cell models, since the specific inhbition of HDAC6 deacetylase activity blocked the recruitment of components of the autophagy machinery to the aggresome. The yet non-elucidated mechanism by which the HDAC6 deacetylase activity is essential to autophagy may be linked to the acetylation levels of HDAC6 substrates . Taken together, these data suggest that an inhibition would not be beneficial for the elimination of protein aggregates in HD. However, the inhibition of HDAC6 stimulated the microtubule-dependent trafficking of vesicles, a process which appears deficient in HD. The inhibition of the deacetylase activity of HDAC6 by TSA and SAHA increased tubulin acetylation and resulted in an improved intracellular vesicle trafficking and release of BDNF. These two processes are disrupted in neurons affected by HD. Even if TSA and SAHA are not specific inhibitors of HDAC6, the authors of this study attributed the observed effects to HDAC6 inhibition based on the fact that MS-275, a specific inhibitor of HDAC1, had no effect on the release of BDNF . Despite these promising results, a study conducted with Hdac6 knockout mice failed to prove the influence of HDAC6 genetic depletion to the clinical manifestations of HD. Even if acetylation levels of tubulin were increased in this model, no effect could be observed in the disease progression. Accordingly, the transport of BDNF from the cortex to the striatum was not improved in this model .
The role of HDAC6 in other neurodegenerative diseases
HDAC6 may also be implicated in several other NDs including FTLD, ALS and CMT [119, 120]. In FTLD and ALS, the down regulation of HDAC6 was observed in a Drosophila model based on the knockout of transactive response DNA-binding protein (TDP-43). Inclusions of TDP-43 are hallmarks of FTDL and ALS, and the silencing of TDP-43 in cells caused HDAC6 downregulation which increased proteotoxicity . On the opposite, in CMT, HDAC6 inhibition could be an interesting therapeutic strategy. Symptomatic improvement was observed in a transgenic mouse model of CMT after the treatment with specific HDAC6 inhibitors, together with the increase in tubulin acetylation .
Critical insights on HDAC6 as a target to fight against neurodegeneration
There is no doubt that HDAC6 is involved in several events of the neurodegenerative cascades and differs from other HDACs not only from a structural point of view, but also in its subcellular localization. Impaired mitochondrial transport and elimination of protein aggregates are common features in various NDs and are linked to both deacetylase and ubiquitin ligase activities of HDAC6. However, it seems that the results obtained with the specific inhibition of HDAC6 in neurodegenerative models cannot be extrapolated from one disease to another. One possible explanation is that different diseases involve specific proteins and the protein-protein interactions (PPI) are not to be neglected in the case of HDAC6. One example is the interaction between HDAC6 and tau, leading to the inhibition of HDAC6 and preventing its role in autophagy . In the case of NDs, PPI mechanisms should be further studied not only in the case of HDAC6, but also in the case of class I HDACs. As a matter of fact, class I HDAC inhibition succeeded to improve cognition, memory and learning in animal models, depending on the disease and the targeted isoform (Additional files). Considering HDAC1 and 2, despite the high level of identity between these isoforms, various outcomes were observed through the overexpression of one enzyme or the other. This may be related to the role of PPI in HDAC activity modulation. In this way, HDAC1 may play a role in neurodegeneration not only via epigenetic mechanisms as HDAC2, but also upon nuclear export followed by its interaction with the nuclear factor CRM-1 and kinesin complex formation in order to intervene with mitochondrial transport .
Even if the specific inhibition of HDAC6 would be beneficial to countervail mitochondrial transport impairement and oxidative stress in NDs [67, 68, 96], as well as tau accumulation  in general cell assays, the role of HDAC6 in protein aggregate elimination was also highlighted . Moreover, WT-161, a selective HDAC6 inhibitor failed to improve cognitive function in a mouse memory test . The in vitro and in vivo results of a specific HDAC6 inhibition (Table 2) did not show an improvement of the cognitive function in NDs. Also, there was a strong evidence that HDAC6 was inhibited by tau in AD . Recently, cognitive improvement was obtained in a mouse model of AD crossed with HDAC6 knockout. Thus, while HDAC6 seems to be necessary for aggregate elimination by autophagy, an induction could also fail to countervail AD pathological conditions, since overexpressed HDAC6 would be eventually inhibited by tau (Figure 3). In this regard, it would be interesting to further investigate PPI between HDAC6 and other proteins involved in specific neurodegenerative processes. PPI inhibitors have been of great interest in drug discovery since they allowed to interact with the specific pathway of an enzyme without interfering with the enzyme activity needed for other processes. Moreover, PPI could be also modulated by small molecules , which is a requested feature in order to allow oral administration and blood–brain barrier permeability. Thus, the study of PPI underlying HDAC6 mechanisms seems to be a promising approach in modulating HDAC6 activity in the context of neurodegeneration.
ATPase associated with a variety of activities
Amyotrophic lateral sclerosis
Protein kinase B (also known as PKB)
Brain-derived neurotrophic factor
Chromosome region maintenance (nuclear receptor)
Parkinson disease (autosomal recessive, early onset) 7 (also known as PARK7)
Glycogen synthase kinase 3-β
Histone deacetylase-like protein
Heat shock factor protein
Heat shock protein
Myocyte enhancing factor-2
Serial analysis of gene expression
Suberoylanilide hydroxamic acid, vorinostat
Tumor necrosis factor-α
Ubiquitin conjugated enzyme
Ubiquitin conjugated enzyme variant
Xu WS, Parmigiani RB, Marks PA: Histone deacetylase inhibitors: molecular mechanisms of action. Oncogene. 2007, 26: 5541-5552. 10.1038/sj.onc.1210620.
Walkinshaw DR, Tahmasebi S, Bertos NR, Yang XJ: Histone deacetylases as transducers and targets of nuclear signaling. J Cell Biochem. 2008, 104: 1541-1552. 10.1002/jcb.21746.
Lahm A, Paolini C, Pallaoro M, Nardi M, Jones P, Neddermann P, Sambucini S, Bottomley M, Lo Surdo P, Carfi A, et al: Unraveling the hidden catalytic activity of vertebrate class Ila histone deacetylases. Proc Natl Acad Sci USA. 2007, 104: 17335-17340. 10.1073/pnas.0706487104.
Boyault C, Zhang Y, Fritah S, Caron C, Gilquin B, Kwon SH, Garrido C, Yao TP, Vourc’h C, Matthias P, et al: HDAC6 controls major cell response pathways to cytotoxic accumulation of protein aggregates. Genes Dev. 2007, 21: 2172-2181. 10.1101/gad.436407.
Haberland M, Montgomery RL, Olson EN: The many roles of histone deacetylases in development and physiology: implications for disease and therapy. Nat Rev Genet. 2009, 10: 32-42. 10.1038/nrg2485.
Dietz KC, Casaccia P: HDAC inhibitors and neurodegeneration: at the edge between protection and damage. Pharmacol Res. 2010, 62: 11-17. 10.1016/j.phrs.2010.01.011.
Dokmanovic M, Clarke C, Marks PA: Histone deacetylase inhibitors: overview and perspectives. Mol Cancer Res. 2007, 5: 981-989. 10.1158/1541-7786.MCR-07-0324.
De Ruijter AJM, van Gennip AH, Caron HN, Kemp S, Van Kuilenburg ABP: Histone deacetylases (HDACs): characterization of the classical HDAC family. Biochem J. 2003, 370: 737-749. 10.1042/BJ20021321.
Fischer A, Sananbenesi F, Mungenast A, Tsai LH: Targeting the correct HDAC(s) to treat cognitive disorders. Trends Pharmacol Sci. 2010, 31: 605-617. 10.1016/j.tips.2010.09.003.
Mai A, Massa S, Pezzi R, Simeoni S, Rotili D, Nebbioso A, Scognamiglio A, Altucci L, Loidl P, Brosch G: Class II (IIa)-selective histone deacetylase inhibitors. 1. Synthesis and biological evaluation of novel (aryloxopropenyl)pyrrolyl hydroxyamides. J Med Assoc Thai. 2005, 48: 3344-3353.
Broide RS, Redwine JM, Aftahi N, Young W, Bloom FE, Winrow CJ: Distribution of histone deacetylases 1–11 in the rat brain. J Mol Neurosc. 2007, 31: 47-58. 10.1007/BF02686117.
Khochbin S, Verdel A, Lemercier C, Seigneurin-Berny D: Functional significance of histone deacetylase diversity. Curr Opin Genet Dev. 2001, 11: 162-166. 10.1016/S0959-437X(00)00174-X.
Gao L, Cueto MA, Asselbergs F, Atadja P: Cloning and functional characterization of HDAC11, a novel member of the human histone deacetylase family. J Biol Chem. 2002, 277: 25748-25755. 10.1074/jbc.M111871200.
Saha RN, Pahan K: HATs and HDACs in neurodegeneration: a tale of disconcerted acetylation homeostasis. Cell Death Differ. 2005, 13: 539-550.
Selvi BR, Cassel JC, Kundu TK, Boutillier AL: Tuning acetylation levels with HAT activators: therapeutic strategy in neurodegenerative diseases. Biochim Biophys Acta. 2010, 1799: 840-853. 10.1016/j.bbagrm.2010.08.012.
Sleiman SF, Basso M, Mahishi L, Kozikowski AP, Donohoe ME, Langley B, Ratan RR: Putting the “HATI” back on survival signalling: the promises and challenges of HDAC inhibition in the treatment of neurological conditions. Expert Opin Inv Drugs. 2009, 18: 573-584. 10.1517/13543780902810345.
Gray S: Targeting Huntington’s disease through histone deacetylases. Clin Epigen. 2011, 2: 257-277. 10.1007/s13148-011-0025-7.
Tang H, Wang XS, Huang XP, Roth BL, Butler KV, Kozikowski AP, Jung M, Tropsha A: Novel inhibitors of human histone deacetylase (HDAC) identified by QSAR modeling of known inhibitors, virtual screening, and experimental validation. J Chem Inf Model. 2009, 49: 461-476. 10.1021/ci800366f.
Li G, Jiang H, Chang M, Xie H, Hu L: HDAC6 α-tubulin deacetylase: a potential therapeutic target in neurodegenerative diseases. J Neurol Sci. 2011, 304: 1-8. 10.1016/j.jns.2011.02.017.
Finnin MS, Donigian JR, Cohen A, Richon VM, Rifkind RA, Marks PA, Breslow R, Pavletich NP: Structures of a histone deacetylase homologue bound to the TSA and SAHA inhibitors. Nature. 1999, 401: 188-193. 10.1038/43710.
Wang DF, Helquist P, Wiech NL, Wiest O: Toward selective histone deacetylase inhibitor design: homology modeling, docking studies, and molecular dynamics simulations of human class I histone deacetylases. J Med Chem. 2005, 48: 6936-6947. 10.1021/jm0505011.
Wang D: Computational studies on the histone deacetylases and the design of selective histone deacetylase inhibitors. Curr Topics Med Chem. 2009, 9: 241-256. 10.2174/156802609788085287.
Vannini A, Volpari C, Di Marco S: Crystal structure of the quorum-sensing protein TraM and its interaction with the transcriptional regulator TraR. J Biol Chem. 2004, 279: 24291-24296. 10.1074/jbc.M401855200.
Khan SN, Khan AU: Role of histone acetylation in cell physiology and diseases: an update. Clin Chim Acta. 2010, 411: 1401-1411. 10.1016/j.cca.2010.06.020.
Gregoretti IV, Lee YM, Goodson HV: Molecular evolution of the histone deacetylase family: functional implications of phylogenetic analysis. J Mol Biol. 2004, 338: 17-31. 10.1016/j.jmb.2004.02.006.
Bertrand P: Inside HDAC with HDAC inhibitors. Eur J Med Chem. 2010, 45: 2095-2116. 10.1016/j.ejmech.2010.02.030.
Witt O, Deubzer HE, Milde T, Oehme I: HDAC family: what are the cancer relevant targets?. Cancer Lett. 2009, 277: 8-21. 10.1016/j.canlet.2008.08.016.
Schuetz A, Min J, Allali-Hassani A, Schapira M, Shuen M, Loppnau P, Mazitschek R, Kwiatkowski NP, Lewis TA, Maglathin RL, et al: Human HDAC7 harbors a class IIa histone deacetylase-specific zinc binding motif and cryptic deacetylase activity. J Biol Chem. 2008, 283: 11355-11363. 10.1074/jbc.M707362200.
Ouyang H, Ali YO, Ravichandran M, Dong A, Qiu W, MacKenzie F, Dhe-Paganon S, Arrowsmith CH, Zhai RG: Protein aggregates are recruited to aggresome by histone deacetylase 6 via unanchored ubiquitin C termini. J Biol Chem. 2012, 287: 2317-2327. 10.1074/jbc.M111.273730.
Ferrante RJ, Kubilus JK, Lee J, Ryu H, Beesen A, Zucker B, Smith K, Kowall NW, Ratan RR, Luthi-Carter R, et al: Histone deacetylase inhibition by sodium butyrate chemotherapy ameliorates the neurodegenerative phenotype in Huntington’s disease mice. J Neurosci. 2003, 23: 9418-9427.
Bertos NR, Gilquin B, Chan GKT, Yen TJ, Khochbin S, Yang XJ: Role of the tetradecapeptide repeat domain of human histone deacetylase 6 in cytoplasmic retention. J Biol Chem. 2004, 279: 48246-48254. 10.1074/jbc.M408583200.
Ding H, Dolan PJ, Johnson GVW: Histone deacetylase 6 interacts with the microtubule-associated protein tau. J Neurochem. 2008, 106: 2119-2130. 10.1111/j.1471-4159.2008.05564.x.
Hook SS, Orian A, Cowley SM, Eisenman RN: Histone deacetylase 6 binds polyubiquitin through its zinc finger (PAZ domain) and copurifies with deubiquitinating enzymes. Proc Natl Acad Sci USA. 2002, 99: 13425-13430. 10.1073/pnas.172511699.
Pai MT, Tzeng SR, Kovacs JJ, Keaton MA, Li SSC, Yao TP, Zhou P: Solution structure of the Ubp-M BUZ domain, a highly specific protein module that recognizes the C-terminal tail of free ubiquitin. J Mol Biol. 2007, 370: 290-302. 10.1016/j.jmb.2007.04.015.
Reyes-Turcu FE, Horton JR, Mullally JE, Heroux A, Cheng X, Wilkinson KD: The ubiquitin binding domain ZnF UBP recognizes the C-terminal diglycine motif of unanchored ubiquitin. Cell. 2006, 124: 1197-1208. 10.1016/j.cell.2006.02.038.
Zhang L, Fang H, Xu W: Strategies in developing promising histone deacetylase inhibitors. Med Res Rev. 2010, 30: 585-602. 10.1002/med.20169.
Estiu G, Greenberg E, Harrison CB, Kwiatkowski NP, Mazitschek R, Bradner JE, Wiest O: Structural origin of selectivity in class II-selective histone deacetylase inhibitors. J Med Chem. 2008, 51: 2898-2906. 10.1021/jm7015254.
Charrier C, Clarhaut J, Gesson JP, Estiu G, Wiest O, Roche J, Bertrand P: Synthesis and modeling of new benzofuranone histone deacetylase inhibitors that stimulate tumor suppressor gene expression. J Med Chem. 2009, 52: 3112-3115. 10.1021/jm9002439.
Kozikowski AP, Tapadar S, Luchini DN, Kim KH, Billadeau DD: Use of the nitrile oxide cycloaddition (NOC) reaction for molecular probe generation: a new class of enzyme selective histone deacetylase inhibitors (HDACIs) showing picomolar activity at HDAC6. J Med Chem. 2008, 51: 4370-4373. 10.1021/jm8002894.
Butler KV, Kalin J, Brochier C, Vistoli G, Langley B, Kozikowski AP: Rational design and simple chemistry yield a superior, neuroprotective HDAC6 inhibitor, tubastatin A. J Am Chem Soc. 2010, 132: 10842-10846. 10.1021/ja102758v.
Schäfer S, Saunders L, Schlimme S, Valkov V, Wagner J, Kratz F, Sippl W, Verdin E, Jung M: Pyridylalanine-containing hydroxamic acids as selective HDAC6 inhibitors. Chem Med Chem. 2009, 4: 283-290.
Kong Y, Jung M, Wang K, Grindrod S, Velena A, Lee SA, Dakshanamurthy S, Yang Y, Miessau M, Zheng C, et al: Histone deacetylase cytoplasmic trapping by a novel fluorescent HDAC inhibitor. Mol Cancer Ther. 2011, 10: 1591-1599. 10.1158/1535-7163.MCT-10-0779.
Schlimme S, Hauser AT, Carafa V, Heinke R, Kannan S, Stolfa DA, Cellamare S, Carotti A, Altucci L, Jung M, et al: Carbamate prodrug concept for hydroxamate HDAC inhibitors. Chem Med Chem. 2011, 6: 1193-1198.
Schäfer S, Saunders L, Eliseeva E, Velena A, Jung M, Schwienhorst A, Strasser A, Dickmanns A, Ficner R, Schlimme S, et al: Phenylalanine-containing hydroxamic acids as selective inhibitors of class IIb histone deacetylases (HDACs). Bioorg Med Chem. 2008, 16: 2011-2033. 10.1016/j.bmc.2007.10.092.
Zou H, Wu Y, Navre M, Sang BC: Characterization of the two catalytic domains in histone deacetylase 6. Biochem Biophys Res Commun. 2006, 341: 45-50. 10.1016/j.bbrc.2005.12.144.
Vecsey CG, Hawk JD, Lattal KM, Stein JM, Fabian SA, Attner MA, Cabrera SM, McDonough CB, Brindle PK, Abel T, et al: Histone deacetylase inhibitors enhance memory and synaptic plasticity via CREB: CBP-dependent transcriptional activation. J Neurosci. 2007, 27: 6128-6140. 10.1523/JNEUROSCI.0296-07.2007.
Fischer A, Sananbenesi F, Wang X, Dobbin M, Tsai LH: Recovery of learning and memory is associated with chromatin remodelling. Nature. 2007, 447: 178-182. 10.1038/nature05772.
Nithianantharajah J, Hannan AJ: Enriched environments, experience-dependent plasticity and disorders of the nervous system. Nat Rev Neurosci. 2006, 7: 697-709. 10.1038/nrn1970.
Peleg S, Sananbenesi F, Zovoilis A, Burkhardt S, Bahari-Javan S, Agis-Balboa RC, Cota P, Wittnam JL, Gogol-Doering A, Opitz L, et al: Altered histone acetylation is associated with age-dependent memory impairment in mice. Science. 2010, 328: 753-756. 10.1126/science.1186088.
Patrick GN, Zukerberg L, Nikolic M, de la Monte S, Dikkes P, Tsai LH: Conversion of p35 to p25 deregulates Cdk5 activity and promotes neurodegeneration. Nature. 1999, 402: 615-622. 10.1038/45159.
Kim D, Frank CL, Dobbin MM, Tsunemoto RK, Tu W, Peng PL, Guan JS, Lee BH, Moy LY, Giusti P, et al: Deregulation of HDAC1 by p25/Cdk5 in neurotoxicity. Neuron. 2008, 60: 803-817. 10.1016/j.neuron.2008.10.015.
Guan JS, Haggarty SJ, Giacometti E, Dannenberg JH, Joseph N, Gao J, Nieland TJF, Zhou Y, Wang X, Mazitschek R, et al: HDAC2 negatively regulates memory formation and synaptic plasticity. Nature. 2009, 459: 55-60. 10.1038/nature07925.
Hawk JD, Florian C, Abel T: Post-training intrahippocampal inhibition of class I histone deacetylases enhances long-term object-location memory. Learn Mem. 2011, 18: 367-370. 10.1101/lm.2097411.
McQuown SC, Barrett RM, Matheos DP, Post RJ, Rogge GA, Alenghat T, Mullican SE, Jones S, Rusche JR, Lazar MA, et al: HDAC3 is a critical negative regulator of long-term memory formation. J Neurosci. 2011, 31: 764-774. 10.1523/JNEUROSCI.5052-10.2011.
Bardai FH, D’Mello SR: Selective toxicity by HDAC3 in neurons: regulation by Akt and GSK3β. J Neurosci. 2011, 31: 1746-1751. 10.1523/JNEUROSCI.5704-10.2011.
Yang XJ, Grégoire S: Class II histone deacetylases: from sequence to function, regulation, and clinical implication. Mol Cell Biol. 2005, 25: 2873-2884. 10.1128/MCB.25.8.2873-2884.2005.
Bolger TA, Yao TP: Intracellular trafficking of histone deacetylase 4 regulates neuronal cell death. J Neurosci. 2005, 25: 9544-9553. 10.1523/JNEUROSCI.1826-05.2005.
Chen B, Cepko CL: HDAC4 regulates neuronal survival in normal and diseased retinas. Science. 2009, 323: 256-259. 10.1126/science.1166226.
Wang WH, Cheng LC, Pan FY, Xue B, Wang DY, Chen Z, Li CJ: Intracellular trafficking of histone deacetylase 4 regulates long-term memory formation. Anatom Rec. 2011, 294: 1025-1034. 10.1002/ar.21389.
Akhtar MW, Raingo J, Nelson ED, Montgomery RL, Olson EN, Kavalali ET, Monteggia LM: Histone deacetylases 1 and 2 form a developmental switch that controls excitatory synapse maturation and function. J Neurosci. 2009, 29: 8288-8297. 10.1523/JNEUROSCI.0097-09.2009.
Kim JY, Casaccia P: HDAC1 in axonal degeneration: a matter of subcellular localization. Cell Cycle. 2010, 9: 3680-3684.
Khan N, Jeffers M, Kumar S, Hackett C, Boldog F, Khramtsov N, Qian X, Mills E, Berghs SC, Carey N, et al: Determination of the class and isoform selectivity of small-molecule histone deacetylase inhibitors. Biochem J. 2008, 409: 581-589. 10.1042/BJ20070779.
Jiang Q, Ren Y, Feng J: Direct binding with histone deacetylase 6 mediates the reversible recruitment of parkin to the centrosome. J Neurosci. 2008, 28: 12993-13002. 10.1523/JNEUROSCI.2860-08.2008.
Su M, Shi JJ, Yang YP, Li J, Zhang YL, Chen J, Hu LF, Liu CF: HDAC6 regulates aggresome-autophagy degradation pathway of α-synuclein in response to MPP+-induced stress. J Neurochem. 2011, 117: 112-120. 10.1111/j.1471-4159.2011.07180.x.
Dompierre JP, Godin JD, Charrin BC, Cordelieres FP, King SJ, Humbert S, Saudou F: Histone deacetylase 6 inhibition compensates for the transport deficit in Huntington’s disease by increasing tubulin acetylation. J Neurosci. 2007, 27: 3571-3583. 10.1523/JNEUROSCI.0037-07.2007.
Iwata A, Riley BE, Johnston JA, Kopito RR: HDAC6 and microtubules are required for autophagic degradation of aggregated huntingtin. J Biol Chem. 2005, 280: 40282-40292. 10.1074/jbc.M508786200.
Parmigiani R, Xu W, Venta-Perez G, Erdjument-Bromage H, Yaneva M, Tempst P, Marks P: HDAC6 is a specific deacetylase of peroxiredoxins and is involved in redox regulation. Proc Natl Acad Sci USA. 2008, 105: 9633-9638. 10.1073/pnas.0803749105.
Chen S, Owens GC, Makarenkova H, Edelman DB: HDAC6 regulates mitochondrial transport in hippocampal neurons. PLoS One. 2010, 5: e10848-10.1371/journal.pone.0010848.
Kozikowski AP, Chen Y, Gaysin A, Chen B, D’Annibale MA, Suto CM, Langley BC: Functional differences in epigenetic modulators - superiority of mercaptoacetamide-based histone deacetylase inhibitors relative to hydroxamates in cortical neuron neuroprotection studies. J Med Chem. 2007, 50: 3054-3061. 10.1021/jm070178x.
Heltweg B, Dequiedt F, Marshall BL, Brauch C, Yoshida M, Nishino N, Verdin E, Jung M: Subtype selective substrates for histone deacetylases. J Med Chem. 2004, 47: 5235-5243. 10.1021/jm0497592.
Nuutinen T, Suuronen T, Kyrylenko S, Huuskonen J, Salminen A: Induction of clusterin/apoJ expression by histone deacetylase inhibitors in neural cells. Neurochem Int. 2005, 47: 528-538. 10.1016/j.neuint.2005.07.007.
Hartl FU, Hayer-Hartl M: Molecular chaperones in the cytosol: from nascent chain to folded protein. Science. 2002, 295: 1852-1858. 10.1126/science.1068408.
Wong E, Cuervo AM: Autophagy gone away in neurodegenerative diseases. Nat Neurosci. 2010, 13: 805-811. 10.1038/nn.2575.
Ciechanover A, Brundin P: The ubiquitin proteasome system in neurodegenerative diseases: Sometimes the chicken, sometimes the egg. Neuron. 2003, 40: 427-446. 10.1016/S0896-6273(03)00606-8.
Wyttenbach A, Arrigo AP: The role of heat shock proteins during neurodegeneration in Alzheimer’s, Parkinson’s and Huntington’s disease. Heat Shock Proteins in Neural Cells. Edited by: Richter-Landsberg C. 2006, Landes Bioscience, Austin
Adams J: The proteasome: a suitable antineoplastic target. Nat Rev Canc. 2004, 4: 349-360. 10.1038/nrc1361.
Ande SR, Chen J, Maddika S: The ubiquitin pathway: an emerging drug target in cancer therapy. Eur J Pharmacol. 2009, 625: 199-205. 10.1016/j.ejphar.2009.08.042.
Bennett EJ, Bence NF, Jayakumar R, Kopito RR: Global impairment of the ubiquitin-proteasome system by nuclear or cytoplasmic protein aggregates precedes inclusion body formation. Mol Cell. 2005, 17: 351-365. 10.1016/j.molcel.2004.12.021.
Taylor JP, Hardy J, Fischbeck KH: Toxic proteins in neurodegenerative disease. Science. 2002, 296: 1991-1995. 10.1126/science.1067122.
Lowe J, Blanchard A, Morrell K, Lennox G, Reynolds L, Billett M, Landon M, Mayer RJ: Ubiquitin is a common factor in intermediate filament inclusion-bodies of diverse type in man, including those of Parkinson’s disease, Pick’s disease, and Alzheimer’s disease, as well as Rosenthal fibers in cerebellar astrocytomas, cytoplasmic bodies in muscle, and mallory bodies in alcoholic liver-disease. J Pathol. 1988, 155: 9-15. 10.1002/path.1711550105.
Dickson DW, Schmidt ML, Lee VMY, Zhao ML, Yen SH, Trojanowski JQ: Immunoreactivity profile of hippocampal CA2/3 neurites in diffuse Lewy body disease. Acta Neuropathol. 1994, 87: 269-276. 10.1007/BF00296742.
Davies SW, Turmaine M, Cozens BA, DiFiglia M, Sharp AH, Ross CA, Scherzinger E, Wanker EE, Mangiarini L, Bates GP: Formation of neuronal intranuclear inclusions underlies the neurological dysfunction in mice transgenic for the HD mutation. Cell. 1997, 90: 537-548. 10.1016/S0092-8674(00)80513-9.
Perry G, Friedman R, Shaw G, Chau V: Ubiquitin is detected in neurofibrillary tangles and senile plaque neurites of Alzheimer disease brains. Proc Natl Acad Sci USA. 1987, 84: 3033-3036. 10.1073/pnas.84.9.3033.
Lindersson E, Beedholm R, Hojrup P, Moos T, Gai WP, Hendil KB, Jensen PH: Proteasomal inhibition by α-synuclein filaments and oligomers. J Biol Chem. 2004, 279: 12924-12934.
Lim KL, Tan J: Role of the ubiquitin proteasome system in Parkinson’s disease. BMC Biochem. 2007, 8: S13-10.1186/1471-2091-8-S1-S13.
Bence NF, Sampat RM, Kopito RR: Impairment of the ubiquitin-proteasome system by protein aggregation. Science. 2001, 292: 1552-1555. 10.1126/science.292.5521.1552.
Keck S, Nitsch R, Grune T, Ullrich O: Proteasome inhibition by paired helical filament-tau in brains of patients with Alzheimer’s disease. J Neurochem. 2003, 85: 115-122. 10.1046/j.1471-4159.2003.01642.x.
Kawaguchi Y, Kovacs JJ, McLaurin A, Vance JM, Ito A, Yao TP: The deacetylase HDAC6 regulates aggresome formation and cell viability in response to misfolded protein stress. Cell. 2003, 115: 727-738. 10.1016/S0092-8674(03)00939-5.
Seigneurin-Berny D, Verdel A, Curtet S, Lemercier C, Garin J, Rousseaux S, Khochbin S: Identification of components of the murine histone deacetylase 6 complex: link between acetylation and ubiquitination signaling pathways. Mol Cell Biol. 2001, 21: 8035-8044. 10.1128/MCB.21.23.8035-8044.2001.
DeLaBarre B, Brunger AT: Complete structure of p97/valosin-containing protein reveals communication between nucleotide domains. Nat Struct Mol Biol. 2003, 10: 856-863. 10.1038/nsb972.
Song C, Xiao Z, Nagashima K, Li CC, Lockett SJ, Dai RM, Cho EH, Conrads TP, Veenstra TD, Colburn NH, et al: The heavy metal cadmium induces valosin-containing protein (VCP)-mediated aggresome formation. Toxicol Appl Pharmacol. 2008, 228: 351-363. 10.1016/j.taap.2007.12.026.
Boyault C, Gilquin B, Zhang Y, Rybin V, Garman E, Meyer-Klaucke W, Matthias P, Muller CW, Khochbin S: HDAC6-p97/VCP controlled polyubiquitin chain turnover. EMBO J. 2006, 25: 3357-3366. 10.1038/sj.emboj.7601210.
Du G, Jiao R: To prevent neurodegeneration: HDAC6 uses different strategies for different challenges. Commun Integr Biol. 2011, 4: 139-142. 10.4161/cib.4.2.14272.
Kalveram B, Schmidtke G, Groettrup M: The ubiquitin-like modifier FAT10 interacts with HDAC6 and localizes to aggresomes under proteasome inhibition. J Cell Sci. 2008, 121: 4079-4088. 10.1242/jcs.035006.
Cook C, Gendron TF, Scheffel K, Carlomagno Y, Dunmore J, DeTure M, Petrucelli L: Loss of HDAC6, a novel CHIP substrate, alleviates abnormal tau accumulation. Human Mol Gen. 2012, 21: 2936-2945. 10.1093/hmg/dds125.
Rivieccio MA, Brochier C, Willis DE, Walker BA, D’Annibale MA, McLaughlin K, Siddiq A, Kozikowski AP, Jaffrey SR, Twiss JL, et al: HDAC6 is a target for protection and regeneration following injury in the nervous system. Proc Natl Acad Sci USA. 2009, 106: 19599-19604. 10.1073/pnas.0907935106.
Olzmann JA, Li L, Chudaev MV, Chen J, Perez FA, Palmiter RD, Chin LS: Parkin-mediated K63-linked polyubiquitination targets misfolded DJ-1 to aggresomes via binding to HDAC6. J Cell Biol. 2007, 178: 1025-1038. 10.1083/jcb.200611128.
Salminen A, Tapiola T, Korhonen P, Suuronen T: Neuronal apoptosis induced by histone deacetylase inhibitors. Mol Brain Res. 1998, 61: 203-206. 10.1016/S0169-328X(98)00210-1.
Leroy K, Yilmaz Z, Brion JP: Increased level of active GSK-3β in Alzheimer’s disease and accumulation in argyrophilic grains and in neurones at different stages of neurofibrillary degeneration. Neuropathol Appl Neurobiol. 2007, 33: 43-55.
Mirra SS, Heyman A, McKeel D, Sumi SM, Crain BJ, Brownlee LM, Vogel FS, Hughes JP, Vanbelle G, Berg L: The Consortium to Establish a Registry for Alzheimers's Disease (CERAD). Part II. Standardization of the neuropathologic assessment of Alzheimers's disease. Neurology. 1991, 41: 479-486. 10.1212/WNL.41.4.479.
Fortin NJ, Agster KL, Eichenbaum HB: Critical role of the hippocampus in memory for sequences of events. Nat Neurosci. 2002, 5: 458-462.
Delacourte A, Sergeant N, Champain D, Wattez A, Maurage CA, Lebert F, Pasquier F, David JP: Nonoverlapping but synergetic tau and APP pathologies in sporadic Alzheimer's disease. Neurology. 2002, 59: 398-407. 10.1212/WNL.59.3.398.
Perez M, Santa-Maria I, De Barreda EG, Zhu X, Cuadros R, Cabrero JR, Sanchez-Madrid F, Dawson HN, Vitek MP, Perry G, et al: Tau - an inhibitor of deacetylase HDAC6 function. J Neurochem. 2009, 109: 1756-1766. 10.1111/j.1471-4159.2009.06102.x.
Govindarajan N, Rao P, Burkhardt S, Sananbenesi F, Schlüter OM, Bradke F, Lu J, Fischer A: Reducing HDAC6 ameliorates cognitive deficits in a mouse model for Alzheimer’s disease. EMBO Mol Med. 2012, 5: 52-63.
Xu J, Kao SY, Lee FJS, Song WH, Jin LW, Yankner BA: Dopamine-dependent neurotoxicity of alpha-synuclein: a mechanism for selective neurodegeneration in Parkinson disease. Nature Med. 2002, 8: 600-606. 10.1038/nm0602-600.
Zarow C, Lyness SA, Mortimer JA, Chui HC: Neuronal loss is greater in the locus coeruleus than nucleus basalis and substantia nigra in Alzheimer and Parkinson diseases. Arch Neurol. 2003, 60: 337-341. 10.1001/archneur.60.3.337.
Perez RG, Waymire JC, Lin E, Liu JJ, Guo F, Zigmond MJ: A role for α-synuclein in the regulation of dopamine biosynthesis. J Neurosci. 2002, 22: 3090-3099.
Masliah E, Rockenstein E, Veinbergs I, Mallory M, Hashimoto M, Takeda A, Sagara Y, Sisk A, Mucke L: Dopaminergic loss and inclusion body formation in α-synuclein mice: implications for neurodegenerative disorders. Science. 2000, 287: 1265-1269. 10.1126/science.287.5456.1265.
Cooper AA, Gitler AD, Cashikar A, Haynes CM, Hill KJ, Bhullar B, Liu K, Xu K, Strathearn KE, Liu F, et al: α-Synuclein blocks ER-Golgi traffic and Rab1 rescues neuron loss in Parkinson’s models. Science. 2006, 313: 324-328. 10.1126/science.1129462.
Du G, Liu X, Chen X, Song M, Yan Y, Jiao R, Wang C: Drosophila histone deacetylase 6 protects dopaminergic neurons against α-synuclein toxicity by promoting inclusion formation. Mol Biol Cell. 2010, 21: 2128-2137. 10.1091/mbc.E10-03-0200.
Savitt JM, Dawson VL, Dawson TM: Diagnosis and treatment of Parkinson disease: molecules to medicine. J Clin Invest. 2006, 116: 1744-1754. 10.1172/JCI29178.
Lee JY, Nagano Y, Taylor JP, Lim KL, Yao TP: Disease-causing mutations in parkin impair mitochondrial ubiquitination, aggregation, and HDAC6-dependent mitophagy. J Cell Biol. 2010, 189: 671-679. 10.1083/jcb.201001039.
Hughes RE, Lo RS, Davis C, Strand AD, Neal CL, Olson JM, Fields S: Altered transcription in yeast expressing expanded polyglutamine. Proc Natl Acad Sci USA. 2001, 98: 13201-13206. 10.1073/pnas.191498198.
Lin CH, Tallaksen-Greene S, Chien WM, Cearley JA, Jackson WS, Crouse AB, Ren SR, Li XJ, Albin RL, Detloff PJ: Neurological abnormalities in a knock-in mouse model of Huntington’s disease. Human Mol Gen. 2001, 10: 137-144. 10.1093/hmg/10.2.137.
Gunawardena S, Her LS, Brusch RG, Laymon RA, Niesman IR, Gordesky-Gold B, Sintasath L, Bonini NM, Goldstein LSB: Disruption of axonal transport by loss of huntingtin or expression of pathogenic PolyQ proteins in Drosophila. Neuron. 2003, 40: 25-40. 10.1016/S0896-6273(03)00594-4.
Gardian G, Browne SE, Choi DK, Klivenyi P, Gregorio J, Kubilus JK, Ryu H, Langley B, Ratan RR, Ferrante RJ, et al: Neuroprotective effects of phenylbutyrate in the N171-82Q transgenic mouse model of Huntington’s disease. J Biol Chem. 2005, 280: 556-563.
Bonini NM: Chaperoning brain degeneration. Proc Natl Acad Sci USA. 2002, 99: 16407-16411. 10.1073/pnas.152330499.
Bobrowska A, Paganetti P, Matthias P, Bates GP: Hdac6 knock-out increases tubulin acetylation but does not modify disease progression in the R6/2 mouse model of Huntington’s disease. PLoS One. 2011, 6: e20696-10.1371/journal.pone.0020696.
D’Ydewalle C, Krishnan J, Chiheb DM, Van Damme P, Irobi J, Kozikowski AP, Vanden Berghe P, Timmerman V, Robberecht W, Van Den Bosch L: HDAC6 inhibitors reverse axonal loss in a mouse model of mutant HSPB1-induced Charcot-Marie-Tooth disease. Nat Med. 2011, 17: 968-974. 10.1038/nm.2396.
Fiesel FC, Voigt A, Weber SS, Van den Haute C, Waldenmaier A, Gorner K, Walter M, Anderson ML, Kern JV, Rasse TM, et al: Knockdown of transactive response DNA-binding protein (TDP-43) downregulates histone deacetylase 6. EMBO J. 2010, 29: 209-221. 10.1038/emboj.2009.324.
Wells JA, McClendon CL: Reaching for high-hanging fruit in drug discovery at protein-protein interfaces. Nature. 2007, 450: 1001-1009. 10.1038/nature06526.
We are thankful to COST action TD0905 (Epigenetics: Bench to Bedside) for allowing exchange of information among scientists in the field of Epigenetics.
Dr Esther Schenker is an employee of Les Laboratoires Servier, France.
CSP designed the first draft with the main ideas to be discussed in this manuscript. CSP and VZ reviewed the literature and drafted the whole manuscript, except for the structural part that was performed by AN. ES gave valuable information in the field of epigenetics and helped to draft the manuscript. PAC and MC coordinated the work and helped to draft the manuscript. All authors read and approved the final manuscript.
Claudia Simões-Pires, Vincent Zwick contributed equally to this work.
Electronic supplementary material
About this article
Cite this article
Simões-Pires, C., Zwick, V., Nurisso, A. et al. HDAC6 as a target for neurodegenerative diseases: what makes it different from the other HDACs?. Mol Neurodegeneration 8, 7 (2013). https://doi.org/10.1186/1750-1326-8-7
- Histone deacetylase
- Neurodegenerative diseases