- Research article
- Open Access
Phosphorylation of tau at Y18, but not tau-fyn binding, is required for tau to modulate NMDA receptor-dependent excitotoxicity in primary neuronal culture
Molecular Neurodegeneration volume 12, Article number: 41 (2017)
Hyperexcitability of neuronal networks can lead to excessive release of the excitatory neurotransmitter glutamate, which in turn can cause neuronal damage by overactivating NMDA-type glutamate receptors and related signaling pathways. This process (excitotoxicity) has been implicated in the pathogenesis of many neurological conditions, ranging from childhood epilepsies to stroke and neurodegenerative disorders such as Alzheimer’s disease (AD). Reducing neuronal levels of the microtubule-associated protein tau counteracts network hyperexcitability of diverse causes, but whether this strategy can also diminish downstream excitotoxicity is less clear.
We established a cell-based assay to quantify excitotoxicity in primary cultures of mouse hippocampal neurons and investigated the role of tau in exicitotoxicity by modulating neuronal tau expression through genetic ablation or transduction with lentiviral vectors expressing anti-tau shRNA or constructs encoding wildtype versus mutant mouse tau.
We demonstrate that shRNA-mediated knockdown of tau reduces glutamate-induced, NMDA receptor-dependent Ca2+ influx and neurotoxicity in neurons from wildtype mice. Conversely, expression of wildtype mouse tau enhances Ca2+ influx and excitotoxicity in tau-deficient (Mapt −/−) neurons. Reconstituting tau expression in Mapt −/− neurons with mutant forms of tau reveals that the tau-related enhancement of Ca2+ influx and excitotoxicity depend on the phosphorylation of tau at tyrosine 18 (pY18), which is mediated by the tyrosine kinase Fyn. These effects are most evident at pathologically elevated concentrations of glutamate, do not involve GluN2B–containing NMDA receptors, and do not require binding of Fyn to tau’s major interacting PxxP motif or of tau to microtubules.
Although tau has been implicated in diverse neurological diseases, its most pathogenic forms remain to be defined. Our study suggests that reducing the formation or level of pY18-tau can counteract excitotoxicity by diminishing NMDA receptor-dependent Ca2+ influx.
Experimental reduction of the microtubule-associated protein tau counteracts neural network hyperexcitability of diverse causes, including in animal models of severe childhood epilepsy and aging-related neurodegenerative disorders such as Alzheimer’s disease [1,2,3,4,5,6,7]. Conversely, neuronal overexpression of tau promotes network hyperexcitability [8,9,10]. Network hyperexcitability can lead to excessive release of excitatory neurotransmitters such as glutamate, which can cause neuronal injury and degeneration by overactivating NMDA-type glutamate receptors (NMDARs) and related signaling pathways (excitotoxicity) [11, 12]. Thus, excitotoxicity may be a key mechanism linking hyperexcitability and tau to neurodegeneration.
Multiple lines of evidence support the notion that tau plays a critical role in NMDAR-dependent excitotoxicity. For example, overexpression of human tau caused excitotoxicity in neuronal cultures . In turn, glutamate-induced excitotoxicity increased tau expression [14, 15] and phosphorylation . However, whereas the ability of tau reduction to suppress network hyperexcitability has been confirmed by multiple groups (see above), the potential of this strategy to suppress excitotoxicity has received less attention. Pizzi et al.  reported that pre-treating cultures of cerebellar granule cells with an anti-tau antisense oligonucleotide (ASO) for 1 h before exposing them to high concentrations of glutamate for 15 min reduced cell loss measured 24 h later. In light of the long half-life of tau [18,19,20], it is not surprising that this brief ASO treatment did not decrease baseline tau levels. Although the ASO did prevent acute, glutamate-induced increases in tau immunostaining, it is possible that its excitoprotective effect resulted from off-target effects. Furthermore, tau reduction in primary neurons did not affect kainate-induced calcium influx , another cause of excitotoxicity. Thus, more work is needed to define the roles of tau and tau reduction in excitotoxicity.
Although the exact mechanisms by which tau reduction counteracts network hyperexcitability, and possibly also downstream excitotoxicity, remain to be defined, several lines of evidence suggest that these mechanisms may involve Fyn. Fyn is a member of the Src family of tyrosine kinases . It can phosphorylate tau at its tyrosine 18 residue to generate pY18-tau  and can bind to tau through one or more proline-rich (PxxP) motifs in tau [23,24,25,26,27]. Fyn can also modulate the phenotype of transgenic mice overexpressing human amyloid precursor proteins (hAPP) and hAPP-derived amyloid-β (Aβ) peptides in neurons. Like humans with AD [28, 29], these mice show evidence for network hyperexcitability, and suppression of this network hyperexcitability by tau reduction or treatment with the antiepileptic drug levetiracetam prevents or ameliorates synaptic and behavioral abnormalities in these models [1, 2, 30]. Neuronal overexpression of Fyn exacerbates network hyperexcitability and behavioral abnormalities in hAPP mice , whereas Fyn ablation reduces their synaptic impairments [32, 33].
Fyn phosphorylates the NMDAR subunit GluN2B at Y1472 [34, 35], which strengthens the interaction between NMDARs and PSD-95 in the postsynaptic density (PSD)  and enhances the activity of GluN2B–containing NMDARs . Genetic ablation of tau in Mapt −/− mice has been reported to decrease the levels of Fyn in neuronal dendrites and synaptosome preparations, and to reduce the level of GluN2B phosphorylation at Y1472 . These and other findings led Ittner et al. to hypothesize that tau enables interactions between Fyn and GluN2B through a process that involves binding of tau to Fyn and that tau reduction suppresses excitotoxicity by preventing Fyn from gaining access to GluN2B . However, consistent with results obtained by others [2, 37], their electrophysiological recordings in acute hippocampal slices revealed that tau ablation did not change excitatory postsynaptic currents (EPSCs) or AMPA/NMDA current ratios, which seems at odds with the proposed hypothesis .
Here we used a primary neuronal culture model to further investigate the roles of tau and tau-Fyn interactions in glutamate-induced excitotoxicity, as well as the extent to which tau reduction can prevent this type of neurotoxicity, which has been implicated in a broad range of neurological diseases [11, 12].
To determine if tau has a role in excitotoxicity, we first established a potentially scalable cell-based assay to quantify excitotoxicity in cultures of primary mouse hippocampal neurons (hereafter referred to simply as “neurons”). For this purpose, we treated neurons with increasing concentrations of glutamate for 15 min, removed the glutamate, and measured reductions in mitochondrial activity 24 h later by alamarBlue assay as a surrogate marker of excitotoxic neuronal injury. Such short-term glutamate treatments are commonly used to study neurotoxicity [38,39,40,41] and could be relevant to the timeframe during which elevations in glutamate levels occur under pathological conditions, for example, as a result of surges in Aβ levels [42,43,44,45] or during seizures .
The alamarBlue assay uses resazurin, an oxidation-reduction indicator , to determine mitochondrial activity, which is altered by exposure of neurons to excitotoxic doses of glutamate [48, 49]. AlamarBlue fluorescence is more sensitive than other indicators of cytotoxicity [50,51,52] and, in neuronal cultures, changes linearly with cell number and decreases in response to toxic concentrations of excitatory amino acids, hypoxia, glucose deprivation, and abnormally low extracellular potassium levels . Because glutamate dose is a critical determinant of excitotoxicity , we ensured that the assay covered a broad range of glutamate concentrations (0–500 μM).
As expected [12, 54], glutamate exposure resulted in dose-dependent neurotoxicity (Fig. 1). Consistent with the prominent role of NMDARs in excitotoxicity  and the mode of action of their antagonists [55,56,57,58,59], the non-competitive NMDAR antagonist MK801 blocked most of the neurotoxicity over a wide range of glutamate concentrations (Fig. 1a), whereas the competitive NMDAR antagonist APV was more effective at moderate than high concentrations of glutamate (Fig. 1b). To assess the relative contribution of GluN2B-containing NMDARs, which have been implicated in excitotoxicity [12, 54], we applied Ro 25–6981 or ifenprodil, two non-competitive antagonists selective for such receptors [60, 61]. These antagonists also were only partly effective (Fig. 1c, d), consistent with other findings suggesting that GluN2B–containing NMDARs contribute to excitotoxicity more markedly at moderate than high doses of NMDAR agonists . DNQX, a competitive antagonist of AMPA receptors (AMPARs), and tetrodotoxin (TTX), a sodium channel blocker, did not significantly attenuate neurotoxicity in our assay (Fig. 1e, f), although both drugs were bioactive (Additional file 1: Figure S1). Depletion of extracellular Ca2+ also shifted the dose-response curve to the right (Additional file 2: Figure S2A), consistent with the dependence of excitotoxicity on Ca2+ influx through NMDARs . These findings agree with those of others indicating that AMPARs and neuronal activity are not required for excitotoxicity to occur [49, 62].
In line with the predominant role of NMDARs in excitotoxicity, the selective NMDAR agonist NMDA was as neurotoxic as glutamate (Additional file 2: Figure S2B). Although blocking AMPARs did not diminish glutamate-induced neurotoxicity (Fig. 1e), two AMPAR agonists, AMPA and L-quisqualic acid, caused intermediate levels of neurotoxicity in our assay (Additional file 2: Figure S2B). In contrast, the kainate receptor (KAR) agonist kainic acid elicited neurotoxicity only at very high doses, and the selective group I mGluR agonist (S)-3,5-DHPG had minimal effects (Additional file 2: Figure S2B).
To evaluate the relation of our neurotoxicity measurements to cell loss, we treated primary neuronal cultures with glutamate as above, confirmed glutamate-induced neurotoxicity by alamarBlue assay 24 h later, fixed and immunostained the cultures for the neuronal marker NeuN  and the astroglial marker glutamine synthetase , and counted the number of immunoreactive cells. Glutamate concentrations between 10 and 100 μM resulted in dose-dependent loss of neurons, but not astrocytes (Fig. 1g, h). In all subsequent experiments, we used the alamarBlue assay to evaluate neurotoxicity because it facilitates the quantitative analysis of multiple neuronal cultures and conditions.
Reduction of tau through lentiviral expression of anti-tau shRNA (shTau) significantly reduced glutamate-induced toxicity in neurons from wildtype but not tau-deficient (Mapt −/−) mice (Fig. 2a-c, Additional file 3: Figure S3), particularly in the glutamate dose range in which we had observed neuronal loss in uninfected wildtype cultures (Fig. 1g, h). Knockdown with shTau reduced endogenous mouse tau (mTau) by ~50% (Fig. 2c). Conversely, lentiviral expression of wildtype 0N4R mTau (mTauWT) exacerbated glutamate-induced neurotoxicity in Mapt −/− but not wildtype neurons (Fig. 2d-f). These results suggest that tau contributes to excitotoxicity, especially in the presence of high glutamate concentrations.
Because Ca2+ influx through NMDARs critically contributes to excitotoxicity (Fig. 1a, Additional file 2: Figure S2A, and [49, 62]), we wondered whether tau might promote this process. To assess this possibility, we measured intracellular Ca2+ levels by live-cell Ca2+ imaging in neurons. We exposed neurons to increasing concentrations of NMDA while blocking voltage-gated Ca2+ channels (VGCC) (Fig. 3a), the other major route of Ca2+ entry into neurons [65, 66]. In this paradigm, tau reduction decreased, whereas tau overexpression increased NMDAR-dependent Ca2+ influx (Fig. 3b, c). Collectively, these results suggest that tau contributes to excitotoxicity, at least in part, by enhancing the cell surface levels or conductance of NMDARs.
How might tau affect NMDARs? Ittner and colleagues proposed that tau-dependent transport of the tyrosine kinase Fyn to the PSD and subsequent phosphorylation of GluN2B by Fyn may modulate neuronal excitability and mediate Aβ-induced neuronal dysfunction . However, Ro 25–6981 did not block the exacerbation of excitotoxicity caused by expression of mTauWT in Mapt −/− neurons (Fig. 4a, b), making it unlikely that the contribution of tau to excitotoxicity depends on GluN2B–containing NMDARs, at least in our cell culture model.
Notably, Fyn has multiple substrates and functions , raising the possibility that interactions between tau and Fyn [23,24,25,26,27] might contribute to Aβ-induced neuronal dysfunction [2, 31,32,33] and excitotoxicity in ways that do not depend on the phosphorylation of GluN2B by Fyn. To test this hypothesis, we constructed several viral vectors that encode 0N4R mTau bearing distinct mutations in putative Fyn interaction domains (Fig. 5a): substituting the single tyrosine residue that can be phosphorylated by Fyn  with phenylalanine (mTauY18F), changing the prolines in each of two potential Fyn-binding PxxP motifs [24,25,26,27] to alanines (mTauAxxA6 and mTauAxxA7, respectively), and deleting both the repeat domain and the C-terminal domain (mTauΔ74). Overexpression of human TauΔ74 (hTauΔ74) has been reported to sequester Fyn from the PSD and to reduce neuronal and cognitive dysfunction in hAPP transgenic mice . To further assess the role of the repeat domain, which is critical for the binding of tau to microtubules , we also generated mTau constructs that lack this domain (mTauΔRD) or have duplicate repeat domains (mTau8RD) [68, 69].
The interaction between tau and Fyn has been studied mostly in a cell-free system  and by co-immunoprecipitation [3, 23] using tagged hTau and an SH2 or SH3 fragment of Fyn [24,25,26,27]. To determine whether mTau interacts with endogenous Fyn in cultured neurons and assess how mutations in mTau affect their interaction, we used a proximity ligation assay (PLA), which enables in situ detection of two antigens in close proximity (<40 nm) (Additional file 4: Figure S4) [68, 70, 71]. In this assay, the antigens are labeled with primary antibodies, which are then bound by two secondary antibodies coupled with DNA strands that can ligate when in close proximity. The ligated sequence serves as an elongation template for quantitative PCR, whose product is labeled and detected by fluorescent probes . Using this assay on Mapt −/− neuronal cultures transfected with the mTau constructs shown in Fig. 5a, we made the following observations. Mutation of Y18 or either PxxP motif reduced the association between total tau and Fyn, whereas deletion of the repeat domain—by itself or in conjunction with the C-terminal domain—did not (Fig. 5b). These findings provide further support for the importance of Y18 and both PxxP motifs in Fyn-tau interactions and suggest that these interactions do not require binding of tau to microtubules. In fact, because mTau8RD bound more tightly to microtubules [68, 69] but less tightly to Fyn (Fig. 5b) than mTauWT, Fyn may interact primarily with tau that is not bound to microtubules and binding of tau to microtubules may diminish its chances of interacting with Fyn.
We also assessed the association of Fyn with tau that is unphosphorylated at residues S198, S199 and S202 (detected with the Tau1 antibody), an interaction that has remained controversial [23,24,25,26,27]. Mutation of Y18 and deletion of the repeat domain increased the association between this type of tau and Fyn, whereas the AxxA7 mutation decreased it, and no significant effects were observed as a result of the AxxA6 mutation, deletion of the repeat domain together with the C-terminal domain, or duplication of the repeat domain (Fig. 5b). These findings suggest that interactions between “unphosphorylated” tau and Fyn depend on only one of the two PxxP motifs and may be diminished by Y18 phosphorylation and binding of tau to microtubules.
All mTau constructs also encoded GFP, which was linked to mTau via a self-cleaving P2A peptide (GFP-P2A-mTau, Additional file 4: Figure S4A). This strategy can be expected to result in the production of mTau and GFP as separate proteins at a 1:1 molar ratio [72,73,74,75]. To control for differences in the expression of constructs across transfected neurons, we used GFP signals to normalize our measurements of mTau levels. Based on these measurements, we found that the AxxA6, Δ74 and ΔRD mutations decreased the relative levels of total and “unphosphorylated” tau, whereas the AxxA7 and 8RD modifications changed the relative abundance of these tau species in different directions (Fig. 5c). Reducing the binding of tau to microtubules (Δ74 and ΔRD) decreased tau levels, consistent with previous findings [3, 68, 69]. Interestingly, the AxxA7 mutation, which markedly reduced interactions of Fyn with both total and “unphosphorylated” tau (Fig. 5b), decreased the levels of total tau but prominently increased the levels of “unphosphorylated” tau (Fig. 5c). Underlying mechanisms may include changes in the stability, turnover or posttranslational modification of the respective tau species and deserve to be further explored.
To determine whether tau-Fyn interactions are involved in the tau-dependent modulation of excitotoxicity, we focused on the Y18F mutation, which disrupts Fyn-dependent phosphorylation of tau at this residue  and the AxxA7 mutation, which interferes with the binding of tau to Fyn (Fig. 5b) [3, 24]. Neurons from Mapt −/− mice were transduced with lentiviral vectors expressing mTauWT, mTauY18F, a phosphomimetic mutant at the Y18 residue (mTauY18E), or mTauAxxA7, followed by exposure to glutamate and the neurotoxicity assay. At >30 μM of glutamate, mTauWT, mTauY18E and mTauAxxA7 similarly increased glutamate-induced neurotoxicity beyond levels in GFP-P2A-expressing tau-deficient neurons, whereas mTauY18F was significantly less effective (Fig. 6a-d). All of these mTau proteins were expressed at comparable levels (Fig. 6e). In Mapt −/− neurons exposed to NMDA in the presence of VGCC blockers, expression of mTauY18F increased NMDAR-dependent Ca2+ influx less than expression of mTauWT (Fig. 6f).
These results suggest that tau promotes glutamate-induced excitotoxicity by increasing NMDAR-dependent Ca2+ influx and that Fyn-mediated phosphorylation of tau at Y18 enhances this process at high concentrations of glutamate. These effects did not involve GluN2B-containing NMDARs and did not require binding of Fyn to tau’s major interacting PxxP motif or of tau to microtubules. In addition to these novel findings, we confirmed that glutamate-induced excitotoxicity is inhibited by 50% reduction of tau [1, 3, 17] and exacerbated by overexpression of tau [9, 10].
We assessed excitotoxicity over a wide range of glutamate concentrations because it is difficult to know how glutamate concentrations in primary neuronal cultures relate to those in the intact or diseased brain. On one hand, the isolation of primary neurons from the brain environment exposes them to many stresses that could increase their susceptibility to excitotoxicity. On the other hand, their culture medium contains numerous neuroprotective factors that could diminish the neurotoxic impact of any given glutamate concentration. In the healthy brain, glutamate is the most abundant free amino acid, but its levels vary widely depending on the exact location. Intracellular glutamate concentrations in the brain are in the millimolar range . The concentration of glutamate in the synaptic cleft ranges from 0.5 to 1.0 mM . The extracellular concentration of glutamate is normally kept in the low micromolar range, for example, at 2 μM in the hippocampus , 9 μM in the cortex , and 10 μM in the CSF . However, glutamate concentrations probably vary more widely in the complex pathological microenvironments of AD brains, the penumbra of an ischemic infarct, and brain regions affected by epileptic seizures. Compared with controls, glutamate levels were increased ~1.3-fold in the CSF of AD patients  and ~2-fold in the extracellular space of the hippocampus of mice overexpressing human APP, which simulate key aspects of AD . Even greater increases in glutamate concentrations may occur in AD brains within and around synaptic clefts: soluble Aβ oligomers, which accumulate in AD brains [81,82,83], elicit glutamate release from astrocytes  and microglia , and prevent excitatory amino acid transporters from clearing extracellular glutamate by negatively affecting their expression or function [43, 84, 85]. Bilateral intrahippocampal microdialysis in humans revealed that extracellular glutamate concentrations can be in the 100–500 μM range for over 15 min during seizures . The experimental conditions under which we observed protective effects of tau reduction may be relevant to some or all of these pathophysiological scenarios.
Indeed, the excitoprotective effects of tau reduction we observed in this study were most evident at pathologically elevated concentrations of glutamate. At lower concentrations of glutamate, tau reduction had no effect on multiple outcome measures, including NMDA-induced Ca2+ influx. These observations are consistent with in vivo studies demonstrating that reduction or ablation of tau has minimal or no effects on neuronal activities unless pathological conditions are introduced that cause network hyperexcitability [1,2,3,4,5,6, 86]. While these studies indicate that tau reduction counteracts network hyperexcitability of diverse causes, the current study suggests that tau reduction may also counteract the excitotoxic neuronal injury to which network hyperexcitability can lead, highlighting the broad potential of this therapeutic strategy.
High concentrations of glutamate are associated with an increased opening time of NMDARs , which may explain, at least in part, why the effects of tau modulation were most evident at high doses of glutamate. It is noteworthy in this context that our measurements may have underestimated tau-dependent changes in NMDAR-dependent Ca2+ influx. We selected FLIPR Calcium 6 (Kd = 320 nM) as an indicator because it has a high quantum yield as well as a broad signal window and amplitude. However, because this indicator can become saturated at high intracellular Ca2+ concentrations, it may not be optimal for detecting changes in Ca2+ influx at the high glutamate concentrations at which tau modulation has the most marked effects on excitotoxicity. Furthermore, our Ca2+ imaging assay measured overall cytosolic Ca2+ levels, whereas the effects of tau modulation on intracellular Ca2+ levels might be confined to specific subcellular regions that are particularly critical for downstream signaling such as the area near the mouth of NMDARs . Although Ca2+ influx plays an important role in excitotoxicity [11, 12, 89], depletion of extracellular Ca2+ only partially protected against excitotoxicity in our model, possibly because we returned our neurons to Ca2+-containing medium right after the glutamate exposure. Ca2+-independent metabotropic functions of NMDA receptors could also contribute to cellular toxicity . It should also be noted that glutamate-induced excitotoxicity may depend less on extracellular and cytoplasmic Ca2+ levels than on mitochondrial Ca2+ levels [11, 91,92,93], which we did not measure. Thus, the relationship between tau, Ca2+ homeostasis, and excitotoxicity is probably complex and deserves further investigation.
The mTauWT-dependent enhancement of glutamate-induced neurotoxicity in our neuronal cultures did not depend on GluN2B–containing NMDARs, as it was observed in the absence (Figs. 2, 4, and 6) or presence (Fig. 4) of Ro-25-6981. Because the NMDAR subunit composition in cultured neurons changes from containing more GluN2B to containing more GluN2A  and our DIV13 neuronal cultures may still be in this transition, we cannot exclude the possibility that this tau effect occurs in maturing, but not adult, neurons. However, DIV12–14 is a standard time point for measuring excitotoxicity [95,96,97], and the GluN2B-to-GluN2A subunit transition at this time point is largely completed [98, 99]. Moreover, treatment with Ro 25–6981 or ifenprodil attenuated glutamate-induced excitotoxicity in our cultures at moderate but not high doses of glutamate, consistent with results obtained in cortical neurons at DIV18 .
Because TauY18F was unable to enhance excitotoxicity at high glutamate concentrations, a potential excitoprotective strategy may be to inhibit phosphorylation of tau by Fyn, a process that appears to be restricted to Y18 . In principle, this could be achieved by inhibiting the kinase activity of Fyn. However, although several inhibitors of other members of the Src kinase family have been developed for the treatment of cancer  and one of these inhibitors is being tested in a clinical trial for AD [101, 102], we are unaware of drugs that selectively inhibit Fyn, but none of the other members of the Src kinase family, and that also effectively penetrate the blood-brain barrier. Furthermore, like the other members of this family, Fyn has diverse substrates and has been implicated in multiple pathways that affect neuronal functions [102,103,104]. Genetic ablation of Fyn in mice did not cause overt phenotypes but reduced calcium flux in thymocytes , impaired LTP in the olfactory bulb and hippocampus [106, 107], and caused spatial learning deficits . Heterozygous Fyn knockout mice had no detectable behavioral or anatomical deficits , suggesting that partial reduction of Fyn may be well tolerated. Indeed, cognitive deficits and synaptic depletion in APP/PS1 mice were reversed by treatment with the Src family kinase inhibitor AZD0530, which inhibits Fyn and is well tolerated in mice, dogs, and humans [101, 102]. Other potential strategies include blocking protein-protein interactions that are required for Fyn to phosphorylate Y18 in tau, and targeting downstream consequences of this posttranslational modification.
The phosphomimetic Y18E substitution in mTau did not increase glutamate-induced neurotoxicity beyond levels observed with mTauWT. Although glutamate is commonly used to mimic tyrosine phosphorylation, it should be noted that glutamate and phosphorylated tyrosine show larger structural differences than other phosphomimetic substitutions (e.g., aspartate versus phosphorylated serine) , which may have contributed, at least in part, to the negative result we obtained with mTauY18E.
Previous studies showed that phosphorylation of Y18 disrupts binding of hTau to Fyn’s SH3 domain  but is required for binding of hTau to Fyn’s SH2 domain . In our PLA experiments, blocking Y18 phosphorylation of mTau by mutagenesis shifted the interaction of Fyn from “total” tau to “unphosphorylated” tau. By extrapolation, it is possible that Y18 phosphorylation promotes the interaction of Fyn with tau species that are also phosphorylated at residues S198, S199 and S202. Additional studies are needed to test this hypothesis and to characterize the intraneuronal distribution of pY18-tau. Other tau phosphorylations have been implicated in the enrichment of tau in dendritic spines and in consequent alterations of NMDARs [109,110,111,112]. In an extensive mass spectrometry analysis of posttranslational tau modifications, we did not detect pY18-tau in hippocampal and cortical homogenates from WT or hAPP transgenic mice , suggesting that this species was of low abundance or absent from these tissues. Because pY18-tau may be confined to a small subcellular compartment, its detection would likely require the development of sensitive pY18-tau-specific antibodies that are suitable for immunostaining of brain sections and electron microscopy; to our knowledge, such antibodies are currently not available. Closely related to the subcellular distribution of pY18-tau is the question of how this tau species might increase NMDAR-dependent Ca2+ influx. Possible mechanisms include interactions of tau with NMDARs, regulators of NMDARs or other component of the PSD, and the modulation of signaling pathways that can increase intracellular Ca2+ levels. Additional studies are needed to explore these nonexclusive possibilities.
Our findings suggest that neuronal tau accumulation promotes and tau reduction counteracts glutamate-induced excitotoxicity, most likely by modulating NMDAR-dependent Ca2+ influx. The tau-dependent enhancement of excitotoxicity requires phosphorylation of tau at its Y18 residue, which is mediated by Fyn, but does not depend on interactions of Fyn with known Fyn-binding domains in tau or on GluN2B/2B–containing NMDARs. Blocking the phosphorylation of tau at Y18 may be excitoprotective, particularly in pathological conditions associated with an abnormal accumulation of tau, glutamate or both.
Table 1 provides information on the antibodies and Table 2 on the pharmacological compounds used in this study.
Primary mouse hippocampal cultures
For the generation of primary cultures, we used male and female WT mice and Mapt −/− mice  (kindly provided by Dr. Hana Dawson, Duke University) on a C57Bl/6 J background. Hippocampi of newborn pups (P0–P1) were dissected in ice-cold Earle’s balanced salt solution (EBSS), lacking CaCl2, MgSO4 and phenol red (Thermo Fisher Scientific, 14155), digested with papain (Worthington LK003176, ~1 unit per hippocampus) in EBSS at 37 °C for 15 min, and triturated in a disposable plastic tube in low ovomucoid solution: Dulbecco’s phosphate-buffered saline (DPBS, Thermo Fisher Scientific, 14040-182) containing 1.5 mg/ml BSA (Sigma-Aldrich, A7030-10G), 1.5 mg/ml trypsin inhibitor (Sigma-Aldrich, T9253-5G), and 66.7 units/ml DNase I (Sigma-Aldrich, D5025). After removing debris with a 70-μm nylon strainer (BD Biosciences, 352350), neurons were spun at 1000 rpm for 5 min. Cell pellets were gently dissociated in Neurobasal A medium supplemented with 1× B27 (Thermo Fisher Scientific, 17504–044), 1× N2 (Thermo Fisher Scientific, 17502–048), 2.4 mM L-glutamine (Thermo Fisher Scientific, 25030–081), and 100 units/ml penicillin/streptomycin (Thermo Fisher Scientific, 15410–122). Cells were plated on poly-D-lysine (PDL)-coated 12-well or 96-well plates at a density of 500,000 or 60,000 neurons/well, respectively, for western blot analyses or glutamate-induced excitotoxicity assay, or 800,000 neurons/coverslip for proximity ligation assays. Half of the medium was replaced with new medium every week. Neurons were used for experiments at DIV 13–15. By DIV14, cultures typically contained similar numbers of neurons and astrocytes (Fig. 1a, h).
Glutamate-induced excitotoxicity assay
Primary cells were plated on PDL-coated 96-well plates (Greiner Bio-one, 655946) at a density of 60,000 cells per well. Cultures were infected with lentiviral constructs encoding shRNA on the day of plating (DIV0) or with lentiviral constructs encoding GFP-P2A or GFP-P2A-mTau on DIV7. On DIV13, cells were treated with various concentrations of glutamate dissolved in Neurobasal A medium with supplements (1× B27, 1× N2, 2.4 mM L-glutamine, and 100 units/ml penicillin/streptomycin) or mock treated with Neurobasal A medium with supplements for 15 min at 37 °C under 5% CO2, followed by incubation conditioned medium previously collected from the same cultures. For Additional file 2: Figure S2A, cells were exposed to glutamate in either Ca2+-containing isotonic solution (vehicle [all in mM]: 138 NaCl, 5 KCl, 1 MgCl2, 2 CaCl2, 10 HEPES, and 10 glucose) or in 0 mM Ca2+ solution (138 NaCl, 5 KCl, 1 MgCl2, 0.1 EGTA, 10 HEPES, and 10 glucose). At 24 h after glutamate treatment, conditioned medium was replaced with pre-warmed HEPES-buffered solution (HBS [all in mM]: 119 NaCl, 2.5 KCl, 2 MgCl2, 2 CaCl2, 25 HEPES, and 30 glucose) containing 1× alamarBlue (Thermo Fisher Scientific, DAL1100), and cultures were incubated at 37 °C under 5% CO2 for 2–3 h. AlamarBlue fluorescence was then measured with a fluorescence plate reader (Molecular Device, SpectraMax M5). Mock treated cells were homogenized in RIPA lysis and extraction buffer (Thermo Fisher Scientific, 89900) with Halt protease and phosphatase inhibitor cocktail (Thermo Fisher Scientific, 78447) to assess tau levels by western blot analysis.
High content imaging
After the excitotoxicity assay, cultures were fixed with 4% paraformaldehyde and 4% sucrose in 1× phosphate buffered saline (PBS) for 15 min at room temperature (RT) and washed three times with 1× PBS. Fixed cells were permeabilized with 0.2% Triton X-100 in 1× PBS for 5 min and blocked with 10% normal goat serum (NGS, Jackson Immunoresearch, 005–000–121) in 1× PBS for 1 h at RT. They were then incubated with primary antibodies against the neuron-specific marker NeuN (Millipore, MAB377) and the glia-specific marker glutamine synthetase (GS, Millipore, MAB302) in 1× PBS containing 2% NGS for 1.5 h at RT. Cultures were then washed three times with 1× PBS at RT and stained with Hoechst33342 (1:5000 dilution, Thermo Fisher Scientific, H3570) to visualize nuclei and with secondary antibodies conjugated with Alexa Fluor546 (A-21123) for NeuN or Alexa Fluor647 (A-21241) for GS (2 μg/ml, Thermo Fisher Scientific) in 1× PBS containing 2% NGS for 1 h at RT. Cultures were then washed three times with 1× PBS and imaged on an ArrayScan XTI Live High Content Platform (Thermo Fisher Scientific). Twenty-one images were taken per well of a 96-well plate using a 10× objective. Filter settings were 549–15 BGRFRN for Alexa Fluor546, 650–13 BGRFRN for Alexa Fluor647, and 386–23 BGRFRN for Hoechst33342. Exposure time for each channel was determined with the built-in peak target (percentile) method, so that target values were between 20% and 25%. After acquiring all images, the number of punctae that were positive for Hoechst33342 and NeuN or GS were counted using the colocalization program of HCS Studio: Cellomics Scan Version 6.5.0 (Thermo Fisher Scientific). Hoechst33342-positive punctae were determined using the following parameters. Smoothing: Uniform (Method: Value = 1), Thresholding: Isodata (Method: Value = −0.25), Segmentation: Shape (Method: Value = 1), and Object cleanup option on. Pre-defined Hoechst33342-positive punctae that were also positive for NeuN or GS were determined using primary object mask modification (Mask type: Circle and Value = 1) and the object selection option by defining the smallest minima in the histograms of both Object.TotalIntensity and Object.AvgIntensity as the low object selection levels and the highest values as the high object selection levels.
Live Ca2+ imaging with Flexstation3
Neurons were plated on PDL-coated 96-well plates (Greiner Bio-one, 655946) at a density of 80,000–120,000 neurons per well, and transduced with lentiviral vectors encoding shRNA on the day of plating (DIV 0) or with lentiviral vectors encoding GFP-P2A or GFP-P2A-mTau at DIV7. At DIV14, neurons were incubated with fluorescent Ca2+ indicator (Molecular Devices, FLIPR Calcium 6 assay kits) in HBSS-Hepes buffer (1× Hank’s balanced salt solution, 20 mM HEPES, 2.5 mM probenecid, 30 mM glucose, and 50 μM glycine) for 1.5 h in the incubator (37 °C, 5% CO2). Voltage gated calcium channel (VGCC) blocker cocktail (ω-conotoxin MVIIC [0.5 μM], SNX482 [0.15 μM], ω -agatoxin IVA [0.1 μM], nimodipine [20 μM], and mibefradil [10 μM]) and Ro 25–6891 were added to some cultures 30 min before the imaging and not washed off. The fluorescence intensity of each well was monitored every 1.28 s for 60 s. HBSS-Hepes buffer containing various concentrations of NMDA was applied 16 s after the beginning of the recording. For each well, the maximal fluorescence increase (ΔF) was normalized to the baseline fluorescence (F0). The ΔF/F0 ratio of each well was divided by the average ΔF/F0 ratio of wells treated with ionomycin (1 μM).
Western blot analysis
Equal amounts of total protein (15 μg per lane) in 1× NuPAGE LDS sample buffer (Thermo Fisher Scientific, NP0007) and 1× Sample Reducing Agent (Thermo Fisher Scientific, NP0009) were loaded per gel lane. Protein samples were electrophoresed on NuPAGE Novex 4–12% Bis-Tris Midi protein gels (Thermo Fisher Scientific, WT1403A) in 1X NuPAGE MOPS SDS running buffer (Thermo Fisher Scientific, NP0001–02) at 200 V for 1 h at RT. Gels were transferred to nitrocellulose membranes with an iBlot gel transfer device (Thermo Fisher Scientific). Membranes were blocked with Odyssey blocking buffer (LI-COR, 927–40000) for 1 h at RT, incubated with primary antibodies overnight at 4 °C, washed with TBS containing 0.05% Tween20 (TBST) four times for 5 min at RT, incubated with matching secondary antibodies conjugated to IRDye (LI-COR, 0.1 μg/mL) for 1 h at RT, and washed in TBST four times for 5 min at RT. Protein bands were visualized with an Odyssey CLx Infrared Imaging System (LI-COR) and quantified with Image Studio software (LI-COR).
Generation of mTau mutants
A cDNA encoding WT 0N4R mTau was linked to a red-shifted variant of GFP (RSGFP4)  via a P2A linker that contained a furin cleavage site and a V5 tag  by PCR. The resulting GFP-P2A-mTau construct was inserted into a pFUW plasmid between AscI and RsrII sites. A short sequence of mTau 3′ UTR (Fragment H) was inserted into this plasmid between RsrII and HpaI sites. Fragment H is homologous to a 3′ UTR region of rat tau that stabilizes tau mRNA and promotes its axonal localization [117, 118]. The RsrII site between mTau and Fragment H was deleted using the Quikchange II XL site-directed mutagenesis kit (Agilent Technologies 200522) and the following primers: 5′-gatccggcgcgccatggtgagcaagg-3′ and 5′-ccttgctcaccatggcgcgccggatc-3′. We refer to the resulting plasmid as GFP-P2A-mTauWT. The GFP-P2A control plasmid lacks the mTau cDNA and has a tga stop codon at the 3′ end of the P2A linker. The Quikchange II XL site-directed mutagenesis kit was used to introduce mutations into GFP-P2A-mTauWT using the primers listed in Table 3.
After mutagenesis, plasmids were validated by sequence analysis and used to transform the Stbl3 E. coli strain (Thermo Fisher Scientific, C7373–03) for maintenance. The constructs encoding mTaunoRD or mTau8RD were described previously .
Production and purification of lentiviral particles
Lentiviral particles were generated by co-transfecting the transfer vector (pFUGW containing shSCR or shTau, and pFUW containing GFP-P2A, GFP-P2A-mTauWT, or GFP-P2A-mTau with mTau mutations), the HIV-1 packaging vector (Delta8.9), and the VSVG envelope glycoprotein expression vector (pVSVG) into HEK293T cells. Confluent HEK293T cells were transfected with 22.5 μg of transfer vector, 16.9 μg of Delta8.9, and 11.25 μg of pVSVG per 15 cm petri dish using CalPhos transfection reagent (Clontech, 631312) according to the manufacturer’s instructions. Medium containing lentiviral particles was collected 48 h after transfection and filtered through a 0.22-μm cellulose acetate filter (Corning Incorporated, 431154). Lentiviral particles in the medium were then concentrated by serial ultracentrifugation: 21,000 rpm for 2 h at 4 °C in a Beckman SW28 and then 25,000 rpm for 2 h at 4 °C in a Beckman SW55 with a sucrose cushion consisting of 2 ml of 20% sucrose in Hank’s balanced salt solution (HBSS, Thermo Fisher Scientific, 14170) at the bottom of the SW55 tubes. Final pellets were dissolved in HBSS, aliquoted, and stored at -80 °C until use. Lentiviral titers were determined with a p24 ELISA. Neuronal cultures were transduced with lentiviral particles encoding shRNA at 3 fg p24 per neuron on the day of plating (DIV0) or with lentiviral particles encoding mTau at 0.02 pg p24 per neuron on DIV7. Lentiviral vectors encoding shRNA against mTau were described previously . Briefly, the target sequence for the anti-tau shRNA was 5′- acagagtccagtcgaagatt -3′. The shRNA was placed under control of the U6 promoter. The U6-shRNA expression cassette (pSilencer 2.0, Ambion) was inserted between the NheI and PacI sites of pFUGW plasmid, upstream of a ubiquitin C promoter directing expression of EGFP. A similar construct expressing an shRNA targeting a scrambled sequence (5′- ccactaccgttgttataggtg -3′) was used as a control.
Proximity Ligation Assay (PLA)
On DIV 7, 106 neurons from Mapt −/− P0–P1 pups were plated on 12-mm poly-D-lysine/laminin-coated glass coverslips (BD Biosciences, 354087), incubated in Neurobasal A medium containing kynurenic acid (1 mM, Sigma-Aldrich, K3375-5G) and GlutaMAX (0.5 mM, Thermo Fisher Scientific, 35050–061) at 37o C with 5% CO2 for 30–60 min, and transfected with pFUW plasmids encoding GFP-P2A or GFP-P2A-mTau. To prepare the transfection mixture, each plasmid (1.0 μg per coverslip) was dissolved in 50 μl Opti-MEM (Thermo Fisher Scientific, 31985–062), mixed with 50 μl Opti-MEM containing 1.35 μl Lipofectamine 2000 (Thermo Fisher Scientific, 11668–027), and incubated for 20 min at RT. Neuronal cultures were incubated in the transfection mixture for 30 min at 37o C with 5% CO2. Cultures were then washed with pre-warmed PBS once and placed back into conditioned medium collected from the same cultures before transfection. PLA was performed according to the protocol of Duolink In Situ/Fluorescence (Sigma-Aldrich) one day after transfection. Neurons were fixed with 4% paraformaldehyde/4% sucrose in 1× PBS for 15 min at RT, washed with 1× PBS (3 × 5 min), permeabilized with 0.2% Triton X-100 in 1× PBS for 5 min, and blocked with 5% normal goat serum (NGS, Jackson Immunoresearch, 005–000–121) in 1× PBS for 30 min at RT. They were then incubated with primary antibodies (Tau1: 1 μg/ml, Tau5: 2 μg/ml, Fyn3: 4 μg/ml) in 1% NGS/1× PBS at 4 °C overnight. After washing with 1× PBS (3 × 5 min), neurons were incubated with two PLA probes (Duolink In Situ PLA Probe Anti-Rabbit PLUS DUO92002 and Duolink In Situ PLA Probe Anti-Mouse MINUS DUO92004, Sigma-Aldrich) in 1% NGS/1× PBS for 1 h at 37 °C, followed by washes (2 × 5 min) with 1× Wash Buffer A (Sigma-Aldrich, DUO82049-4 L) and incubation in ligation solution (Sigma-Aldrich, Duolink In Situ Detection Reagents Red, DUO92008) for 1 h at 37 °C. After ligation of complementary PLA probes that were in close (<40 nm) proximity, neurons were washed with 1× Wash Buffer A (2 × 2 min) and incubated in the amplification solution (Sigma-Aldrich, Duolink In Situ Detection Reagents Red, DUO92008) for 100 min at 37 °C to fluorescently label the ligated PLA probes. Neurons were then serially washed in 1× Wash Buffer B (2 × 10 min, Sigma-Aldrich, DUO82049-4 L), 0.01× Wash Buffer B (1 × 1 min), and 1× PBS (1 × 5 min), followed by incubation with secondary antibodies (Thermo Fisher Scientific, Alexa-Fluor647 Goat anti Mouse, A21235) in 1% NGS/1× PBS for 1 h at RT. Finally, neurons were washed with 1× PBS (3 × 5 min) and mounted in Duolink In Situ Mounting Medium with DAPI (Sigma-Aldrich, DUO 82040). Fluorescent images were obtained with an epifluorescence microscope (Nikon Ti-E Microscope, Nikon Imaging Center at UCSF). The intensity of PLA signals, Tau1 and Tau5 immunoreactivity, and GFP fluorescence within neuronal processes were quantitated with the NIS elements software (Nikon). Since PLA signals correlated with the expression levels of mTau (Additional file 4: Figure S4C), they were normalized to colocalized Tau1 or Tau5 immunofluorescence to calculate the affinity of each mTau mutant to Fyn.
Experimenters were blinded with respect to the genotype and treatment of cell cultures. Biological units were randomized during assays, sampling and analyses. Statistical analyses were performed with Prism (Ver. 6, GraphPad) or R (R Development Core Team, http://www.R-project.org/). Individual experiments (glutamate-induced excitotoxicity assay, live Ca2+ imaging, high-content imaging assay, and western blot analysis) or individual transfected neurons (PLA) were treated as independent units (n). Across experiments/plates, neurons of the same genotype that were exposed to the same experimental condition showed a reasonably consistent, dose-dependent change in alamarBlue signal or intracellular Ca2+ signal upon treatment with glutamate or other drugs. However, across experiments/plates we observed systematic variations in the mean and variance of neuronal responses as well as in the shapes of dose-response curves (Additional file 3: Figure S3). Therefore, we considered different wells on the same plate technical replicates and defined independent experiments as independent units (n) for statistical analysis. To analyze data obtained in multiple experiments, differences in the mean fluorescence signal intensities obtained by alamarBlue or Ca2+ imaging in wells containing experimentally altered cells (e.g., transduced with shTau) versus control cells (e.g., transduced with shSCR) on the same plate were computed for each dose of glutamate or another drug. The null hypothesis tested was that the median of the above differences at each dose was less than or equal to 0 across experiments/plates. To compare two different experimental conditions across different doses of glutamate, we calculated a p-value for an overall interaction between two conditions and dose from a one-sided, one-sample t-test performed on the mean difference in alamarBlue or intracellular Ca2+ signal intensities between the two experimental conditions across all doses. The above statistical approach was selected because it is non-parametric, and thus does not assume any particular systematic variation in the responses among experiments/plates or any particular parametric shape of the dose response curve. In other experiments, differences between genotypes and treatments were assessed, as appropriate, by unpaired Student’s t-test with Welch’s correction or by one-way ANOVA. The null hypothesis was rejected at P < 0.05. Quantitative data are presented as boxplots or means ± SEM.
Anti-tau antisense oligonucleotide
- Ca2+ :
Excitatory postsynaptic currents
Human amyloid precursor proteins
Tau phosphorylated at tyrosine 18
Proline-rich motifs in tau
- Mapt −/− :
Voltage-gated Ca2+ channels
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We thank Grisell Diaz-Ramirez for advice on statistical analyses, Jordan Xu for technical assistance, Michael Ward for providing the GFP-P2A construct, Eckhard Mandelkow for cDNA encoding 0N4R murine tau, Jennifer Whistler and Li Fei for protocols for live Ca2+ imaging, Yadong Huang and Reeny Balestra for access to the FlexStation3, Gary Howard for editorial review, Amy Cheung, Courtney Dickerson, and Joy Lingat for administrative assistance, Elsa Suberbielle for designing primers to generate the Y18F and AxxA7 mutations, David Chung for carrying out the p24 ELISA, and Steve Finkbeiner for helpful comments on the manuscript.
This work was supported by NIH grants NS041787 to LM and K23 AG038357 to KV. TM was supported by fellowships from the Japan Society for the Promotion of Science and the Uehara Memorial Foundation. LS was supported by Ruth L. Kirschstein National Research Service Award F32 NS093766. The Gladstone Institutes received support from a National Center for Research Resources Grant RR18928.
Availability of supporting data
The datasets supporting the conclusions of this article are included within the article and its additional files. Additional datasets during and/or analysed during the current study are available from the corresponding author upon reasonable request.
TM performed most experiments and analysis, interpreted the data, and wrote the manuscript; LS interpreted the data, performed experiments, and wrote the manuscript; RT designed and performed the statistical analysis and wrote the manuscript; BD performed electrophysiological recordings, interpreted the data, and wrote the manuscript; PT interpreted the data, contributed to the statistical analysis and wrote the manuscript; JK contributed to the proximity ligation assay; KV created mutant, truncated, and duplicate-repeat-domain mTau constructs and interpreted the data; LM conceived of and supervised the study, interpreted the data, and wrote the manuscript. All authors read and approved the final manuscript.
LM is a co-inventor on tau-related patents owned by the Gladstone Institutes. He is or has been the principal investigator of sponsored research collaborations of the Gladstone Institutes with Bristol-Myers Squibb and with Cure Network Dolby Acceleration Partners. In addition, TM is currently an employee of Denali Therapeutics. The other authors declare that they have no competing interests.
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Mice were treated in accordance with guidelines set by the Institutional Animal Care and Use Committee of the University of California, San Francisco.
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Additional file 1: Figure S1.
DNQX and TTX were bioactive. (A–C) Experiments illustrating effects of DNQX (A) and TTX (B, C). (A) A single experiment was performed to confirm that DNQX blocks AMPA-induced neurotoxicity in primary neuronal cultures. Data represent means ± SEM of technical replicates (4–6 wells per condition). WT neurons were treated with vehicle or the competitive AMPAR antagonist DNQX (20 μM) 1 h before and throughout exposure to different concentrations of AMPA. Raw AlamarBlue fluorescence measurements are shown in arbitrary units. (B, C) TTX blocked spontaneous and induced neuronal activity in acute brain slices. Slices of somatosensory cortex were prepared and pyramidal neurons were patch-clamped as described . Whole-cell membrane voltage recordings were made in artificial cerebrospinal fluid (aCSF) in the absence or presence of 3 μM TTX (from the same batch used for cell culture experiments). (B) TTX prevented spontaneous neuronal activity as well as activity induced by 100 pA/200 ms current stimulations (arrowheads) or by a membrane voltage ramp (between asterisks). Scale bar indicates 20 s of recording time. (C) Representative membrane voltage responses elicited by 100 pA/200 ms current stimulations in control aCSF and after addition of 3 μM TTX. (PDF 4818 kb)
Additional file 2: Figure S2.
Glutamate-induced neurotoxicity depends on NMDARs and extracellular Ca2+. (A, B) Experiments illustrating Ca2+ dependence of glutamate-induced neurotoxicity (A) and neurotoxicity caused by different glutamate receptor agonists (B). (A) One experiment was performed to confirm that reduction of extracellular Ca2+ counteracts glutamate-induced neurotoxicity. WT neurons were treated with Ca2+-containing isotonic solution or 0 mM Ca2+ solution 1 h prior to and throughout exposure to different concentrations of glutamate. Raw AlamarBlue fluorescence measurements are shown in arbitrary units. (B) Neurotoxicity was assessed as above in WT neuronal cultures treated with the indicated glutamate receptor agonists for 15 min at DIV 13. Data represent means ± SEM of technical replicates (8–16 wells per condition) from 1 to 2 experiments. (PDF 4803 kb)
Additional file 3: Figure S3.
Variability of glutamate-induced neurotoxicity measurements across independent experiments. In each of seven independent experiments (carried out on different days), primary neurons pooled from multiple WT mice were cultured on a single 96-well plate and transduced with lentiviral vectors encoding shSCR or shTau (44–48 wells per shRNA) at the time of plating (DIV0). On DIV 13, neurons were exposed to glutamate (4–8 wells per concentration) for 15 min, followed by alamarBlue assay of neurotoxicity 24 h later. Different from the other figures, the box plots shown here represent the distribution of the raw fluorescence signals (in arbitrary units) measured in each set of wells (technical replicates) per glutamate concentration for each experimental condition (shSCR and shTau) and experiment (1–7). The lower and upper ends of the boxes represent the 25th and 75th quartile of the respective distributions. The horizontal line in each box represents the median. The ends of the whiskers terminate at the farthest points that are within 1.5 times the inter-quartile range (difference between upper and lower ends of the box). Individual dots shown in some of the panels represent outliers that fell outside the range defined by the whiskers. Note that, across experiments, neurons of the same genotype that were exposed to similar experimental conditions showed systematic variations in the mean and variance of their responses at given doses as well as in the shapes of their dose-response curves. Therefore, accounting for experiment-to-experiment variability by mean models of intensity (e.g., linear mixed effect models) and minimizing the number of dose-specific parameters to be estimated by assuming dose-response models (e.g., linear, cubic, or Hill) were not suitable for the analysis of this data. (PDF 4937 kb)
Additional file 4: Figure S4.
Proximity ligation assay (PLA) to quantify association between tau and Fyn in neurons. (A) Diagram illustrating key features of the PLA assay. Neuronal cultures from Mapt −/− mice were transfected on DIV7 with a plasmid encoding GFP-P2A or GFP-P2A-mTau and fixed for PLA and immunostaining on DIV8. The GFP-P2A-mTau fusion protein is posttranslationally cleaved at the C-terminal side of the P2A peptide, resulting in the production of GFP-P2A and mTau at a 1:1 molar ratio. The mTau expressed was either WT or mutant (Fig. 5a). GFP signals were used to identify transfected neurons and PLA signals to measure the association between exogenous mTau and endogenous Fyn (see Methods for details). mTau immunofluorescence was used to normalize PLA signals, because mTau expression levels varied among neurons and correlated with PLA signals, as indicated in (C). (B, C) Neurons were transfected with plasmids encoding GFP-P2A, GFP-P2A-mTauWT, or GFP-P2A-mTauAxxA7 on DIV7 and analyzed by PLA and tau immunostaining on DIV8. (B) Representative photomicrographs showing levels of GFP signals (left), PLA signals (middle), and tau immunoreactivity (right) in three neurons from different culture wells. Scale bar: 50 μm. (C) Correlation between PLA signals and Tau5 immunofluorescence across individual neurons in cultures transfected with GFP-P2A-mTauWT (black: R2 = 0.6436, P < 0.0001) or GFP-P2A-mTauAxxA7 (red: R2 = 0.3098, P < 0.0001) by linear regression analysis. Neurons transfected with GFP-P2A (gray) served as a negative control. Data points represent measurements obtained in individual neurons (mean of signals from three neurites per neuron) on two coverslips in a single experiment. (PDF 7504 kb)
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Miyamoto, T., Stein, L., Thomas, R. et al. Phosphorylation of tau at Y18, but not tau-fyn binding, is required for tau to modulate NMDA receptor-dependent excitotoxicity in primary neuronal culture. Mol Neurodegeneration 12, 41 (2017). https://doi.org/10.1186/s13024-017-0176-x