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Targeting Hif1a rescues cone degeneration and prevents subretinal neovascularization in a model of chronic hypoxia

Molecular Neurodegeneration volume 13, Article number: 12 (2018) Cite this article

Abstract

Background

Degeneration of cone photoreceptors leads to loss of vision in patients suffering from age-related macular degeneration (AMD) and other cone dystrophies. Evidence, such as choroidal ischemia and decreased choroidal blood flow, implicates reduced tissue oxygenation in AMD pathology and suggests a role of the cellular response to hypoxia in disease onset and progression. Such a chronic hypoxic situation may promote several cellular responses including stabilization of hypoxia-inducible factors (HIFs).

Methods

To investigate the consequence of a chronic activation of the molecular response to hypoxia in cones, von Hippel Lindau protein (VHL) was specifically ablated in cones of the all-cone R91W;Nrl-/- mouse. Retinal function and morphology was evaluated by ERG and light microscopy, while differential gene expression was tested by real-time PCR. Retinal vasculature was analyzed by immunostainings and fluorescein angiography. Two-way ANOVA with Šídák’s multiple comparison test was performed for statistical analysis.

Results

Cone-specific ablation of Vhl resulted in stabilization and activation of hypoxia-inducible factor 1A (HIF1A) which led to increased expression of genes associated with hypoxia and retinal stress. Our data demonstrate severe cone degeneration and pathologic vessel growth, features that are central to AMD pathology. Subretinal neovascularization was accompanied by vascular leakage and infiltration of microglia cells. Interestingly, we observed increased expression of tissue inhibitor of metalloproteinase 3 (Timp3) during the aging process, a gene associated with AMD and Bruch’s membrane integrity. Additional deletion of Hif1a protected cone cells, prevented pathological vessel growth and preserved vision.

Conclusions

Our data provide evidence for a HIF1A-mediated mechanism leading to pathological vessel growth and cone degeneration in response to a chronic hypoxia-like situation. Consequently, our results identify HIF1A as a potential therapeutic target to rescue hypoxia-related vision loss in patients.

Background

Age-related macular degeneration (AMD) is the leading cause of visual impairment in the elderly population in industrialized nations [1,2,3]. Due to the degeneration of photoreceptors in the cone-rich macula and/or the ingrowth of blood vessels, patients suffering from AMD lose central, high acuity vision [4,5,6]. While there is no therapy available for geographic atrophy (dry AMD), the neovascular (wet) form of AMD is treated by vascular endothelial growth factor (VEGF)-targeting therapies to slow disease progression [7,8,9]. Choroidal neovascularization (CNV) defines the classic form of neovascular AMD and is characterized by the ingrowth of blood vessels from the choroid to the subretinal space [6, 10]. Retinal angiomatous proliferation (RAP) has been described as an additional, distinct form of neovascular AMD [11]. In RAP, also known as deep retinal vascular anomalous complexes, vessels originate not from the choroid but from the deep retinal plexus in the inner retina and extend into the photoreceptor layer and the subretinal space [12, 13].

AMD is a multifactorial disease. Besides genetic and environmental risk factors [5, 14, 15], tissue hypoxia and changes in retinal blood flow have been implicated in its etiology [16,17,18,19,20]. Oxygen supply to photoreceptors in the eyes of elderly people may be impaired due to an age-dependent reduction of choroidal blood flow [21, 22] and accumulation of drusen [23]. Choroidal ischemia in dry AMD [24, 25] and decreased choroidal blood volume in AMD [26] further support the hypothesis that hypoxia might be implicated in disease development and progression. The retina is considered as one of the most metabolically active tissues and is therefore highly vulnerable to changes in oxygen tension [17, 27]. In conditions of reduced oxygen supply (hypoxia), molecular responses are activated with hypoxia-inducible factor 1 (HIF1) playing a key regulatory role for adapting the cell/tissue to the new condition. Heterodimeric HIF1 proteins are composed of an oxygen-labile α-subunit and a constitutively expressed β-subunit [28]. Under normoxic conditions, HIF1A is hydroxylated by prolyl hydroxylase domain (PHD) proteins. This promotes the interaction with the von Hippel-Lindau (VHL) ubiquitin E3 ligase complex leading to ubiquitination and rapid degradation of hydroxylated HIF1A by proteasomes. Under hypoxic conditions, hydroxylation of HIF1A is reduced. Hence, HIF1A accumulates, enters the nucleus and drives transcription of a multitude of target genes [29,30,31].

In this study, we investigated the consequences of a chronic hypoxia-like response triggered in cone photoreceptors to elucidate the mechanisms of cell death in cone degenerative diseases such as AMD. To this end, we used R91W;Nrl-/- double-mutant mice which express only cone photoreceptors in a well-layered, functional retina [32]. To induce the hypoxia-like response, we ablated the VHL protein specifically from cones using the Cre-loxP system. We analyzed the effects of the cone-specific activation of the hypoxic response and validated the contribution of HIF1A to the resulting retinal pathology.

Methods

Mice

All experimental procedures were performed according to ‘The Association for Research in Vision and Ophthalmology’ statement on animal use in ophthalmic and vision research and the regulation of the veterinary authorities of Kanton Zurich, Switzerland. R91W;Nrl-/- mice were generated by crossing Rpe65R91W (R91W) [33] to Nrl-/- mice [34], and were described recently [32]. BPCre;R91W;Nrl-/-;Vhlf/f (=coneΔVhl) mice were generated by breeding R91W;Nrl-/- mice to Vhlf/f mice [35] and mice expressing the Cre recombinase under the transcriptional control of the blue cone opsin (BP) promoter [36]. To generate BPCre;R91W;Nrl-/-;Vhlf/f;Hif1af/f (=coneΔVhlHif1a) mice, BPCre;R91W;Nrl-/-;Vhlf/f were bred to Hif1af/f mice [37]. R91W;Nrl-/-;Vhlf/f and R91W;Nrl-/-;Vhlf/f;Hif1af/f littermates without Cre recombinase served as respective control mice (=ctrl). Genotyping was performed by PCR using DNA isolated from ear clips and primer pairs as described previously [38, 39]. Presence of BPCre was tested using the following primer pair: forward (5’-GGACATGTTCAGGGATCGCCAGGCG-3’) and reverse (5’-GCATAACCAGTGAAACAGCATTGCTG-3’). The amplification reaction resulted in a 268 bp fragment in the presence of the transgene. To test for deletion of floxed sequences, genomic DNA was isolated from retinal tissues and tested by PCR using appropriate primer pairs as described [38, 39]. 129S6 (Taconic, Ejby, Denmark) mice were used as wild-type controls. To test expression of the Cre recombinase BPCre;R91W;Nrl-/- mice were bred to a ZsGreen reporter line (Ai6 mice, Gt(ROSA)26Sortm6(CAG-ZsGreen1)Hze, [40]). Mice of both sexes were used for experiments and were housed at the animal facility of the University Zurich under a 14 h : 10 h light/dark cycle with lights on at 6 am and lights off at 8 pm. Food and water were provided ad libitum.

Morphology/quantification

To evaluate retinal morphology, eyes were enucleated and fixed in 2.5% glutaraldehyde in cacodylate buffer (pH 7.2, 0.1 M), according to the previously described procedure [41]. By cutting through the optic nerve head, nasal and temporal halves of the eyecups were separated and embedded in epon plastic. Semi-thin cross sections (0.5 μm) were counterstained with toluidine blue and analyzed by light microscopy (Axioplan; Zeiss, Jena, Germany). Thickness of the outer nuclear layer was measured at indicated distances from the optic nerve head using the Adobe Photoshop CS6 ruler tool (Adobe Systems, Inc., San Jose, CA, USA) on reconstructed retinal panorama images.

Immunofluorescence

After euthanasia, eyes were marked nasally, enucleated and fixed in 4% paraformaldehyde (PFA) in phosphate buffer for 1 h at 4°C. Cornea and lens were removed and the dissected eyecups postfixed for 2 h in 4% PFA. After immersion in 30% sucrose (in PBS 0.1 M) the eyecups were embedded in tissue freezing medium (O.C.T., Leica Biosystems Nussloch GmbH, Nussloch, Germany), frozen in a 2-methylbutane bath cooled by liquid nitrogen and stored at -80°C. Cryosections (12 μm) were blocked (3% normal goat serum (Sigma-Aldrich, St. Louis, MO, USA), 0.3% Triton X-100 (Sigma) in PBS) and incubated with the following primary antibodies overnight at 4°C: Isolectin GS-IB4-Alexa594 from Griffonia simplicifolia (1:300, I21413; Thermo Fisher Scientific, Waltham, MA, USA), rabbit anti-allograft inflammatory factor 1 (alias IBA1, 1:1000, 019-19741; Wako, Neuss, Germany), rabbit anti-albumin (ALB, 1:500, RARaAlb; Nordic Immunology, Tilburg, Netherlands). Sections were washed with PBS, incubated with secondary antibodies (Cy3-labeled, Jackson ImmunoResearch Laboratories, Westgrove, PA, USA) for 1 h at room temperature (RT), counterstained with DAPI (4',6-Diamidine-2'-phenylindole dihydrochloride, Roche, Basel, Switzerland) and analyzed by fluorescence microscopy (Axioplan; Zeiss).

Analysis of retinal vasculature in whole mounted retinas

Eyes were isolated and incubated for 5 to 10 minutes in 2% PFA in PBS as described recently [42]. After removal of cornea and lens, the retina was dissected and flat-mounted in PBS. After postfixation in 4% PFA for 1 h at RT, flat-mounts were blocked (3% normal goat serum, 0.3% Triton X-100 in PBS, 1 h) and incubated with isolectin GS-IB4-Alexa594 (1:300, Thermo Fisher Scientific) at 4°C overnight. Retinas were washed in PBS, mounted on glass slides and analyzed by fluorescence microscopy (Axioplan/ApoTome; Zeiss). Blood vessels were reconstructed in three dimensions using Imaris software (Versions 7.7.2/8.3.0, Bitplane AG, Zurich, Switzerland). For better recognition and distinction of the vascular plexi, the z-value of the z-stacks was increased five times.

RNA isolation and semi-quantitative real-time PCR

Retinas were isolated through a slit in the cornea, frozen in liquid nitrogen and stored at -80°C. RNA was extracted using an RNA isolation kit (RNeasy; Qiagen, Hilden, Germany) including a DNAse treatment. 1 μg of RNA, oligo (dT) and M-MLV reverse transcriptase (Promega, Fitchburg, WI, USA) were used to prepare cDNA. To analyze gene expression by real-time PCR, 10 ng of cDNA template was amplified using a PCR polymerase ready mix (LightCycler 480 SYBR Green I Master, Roche Diagnostics, Rotkreuz, Switzerland), specific primer pairs (Table 1) and a thermocycler (LightCycler 480, Roche Diagnostics). Expression levels were normalized to β-actin (Actb) and relative expression was calculated using the comparative threshold cycle method (∆∆CT) of the LightCycler480 software (Roche Diagnostics). At least 3 mice per strain and time point were used.

Table 1 Primers used for real-time PCR
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Protein isolation and Western blotting

Proteins were isolated by homogenizing the retinas in ice-cold Tris-HCl (100 mM, pH 7.5) using ultrasound at 4°C. Protein concentrations were determined spectrophotometrically by using Bradford reagent (Bio-Rad, Hercules, CA, USA). SDS-PAGE and Western blotting were performed as described [41] and proteins detected by rabbit anti-HIF1A (1:2000, NB100-479, Novus Biologicals, Littleton, CO, USA) and mouse anti-β-actin (1:10’000, A5441, Sigma-Aldrich) antibodies. HRP-conjugated secondary antibodies were applied for 1 h at RT and signals were visualized using Western lightning chemiluminescence reagent (PerkinElmer, Waltham, MA, USA) and X-ray films.

Electroretinography (ERG)

Mice were dark-adapted overnight and pupils were dilated under dim red light with 1% cyclogyl (Alcon Switzerland SA, Rotkreuz, Switzerland) and 5% neosynephrin-POS (Ursapharm Schweiz GmbH, Roggwil, Switzerland) 30 minutes prior to recording. Mice were anesthetized with a subcutaneous injection of ketamine (85 mg/kg, Pfizer PFE Switzerland GmbH, Zurich, Switzerland) and xylazine (Rompun 2%, 4mg/kg, Bayer, Leverkusen, Germany). To keep the cornea moist, a drop of mydriaticum dispersa (Omnivision AG, Neuhausen, Switzerland) was applied to each eye. Low background illumination for 5 min was used for light adaptation. Electroretinograms were recorded simultaneously from both eyes with an LKC UTAS Bigshot unit (LKC Technologies, Inc. Gaithersburg, MD, USA) as described [32]. Flashes of 8 different light intensities ranging from -10 to 25 dB (0.25–790.5694 cd*s/m2) were applied under photopic conditions. 10 responses were averaged per light intensity. Traces from n≥4 mice were averaged for each light intensity.

Fundus imaging and fluorescein angiography

Fundus photographs were taken with a Micron IV system (Phoenix Research Labs, Pleasanton, CA, USA). The pupils were dilated and the mice were anesthetized as described above. Methocel 2% (Omnivision) was applied to lubricate the eyes. Fluorescein images were captured 1-5 minutes after intraperitoneal injection of 20 μL of 2% fluorescein solution (Akorn, Lake Forest, IL, USA).

Experimental design and statistical analysis

Two-way ANOVA with Šídák’s multiple comparison test was performed using GraphPad Prism (version 7.02, GraphPad Software, San Diego, CA, USA) for statistical analysis of gene expression levels and ERG traces. All data are shown as means ± SD.

Results

Cone-specific inactivation of Vhl increased HIF1A target gene expression and induced progressive cone degeneration

Photoreceptors in the all-cone R91W;Nrl-/- mouse are predominantly blue-light sensitive S-cones [32, 34]. Therefore, we used BP-Cre transgenic mice, which express functional Cre recombinase under the transcriptional control of the blue cone opsin promoter (BP, [36]) to delete floxed sequences from cones in the all-cone mice. BPCre;R91W;Nrl-/-;ZsGreen reporter mice verified that Cre expression was uniform over the entire retina and restricted almost exclusively to the majority of cones in the ONL (Fig. 1a). Green fluorescence of the activated reporter protein was only occasionally detected in few cells of the inner retina. We validated Vhl excision in the BPCre;R91W;Nrl-/-;Vhlf/f (=coneΔVhl) mouse line by analyzing genomic DNA isolated from retinal tissues of 4-week-old coneΔVhl mice. The detection of a PCR product corresponding to the excised fragment suggested a successful Cre-mediated deletion of Vhl in retinas of coneΔVhl but not of ctrl mice (=R91W;Nrl-/-;Vhlf/f, Fig. 1b). Genomic deletion of Vhl led to the accumulation of HIF1A protein (Fig. 1c) and to increased transcript levels of known hypoxic target genes such as adrenomedullin (Adm), egl-9 family hypoxia-inducible factor 1 (Egln1, also known as Phd2), glucose transporter 1 (Glut1) and BCL2/adenovirus E1B 19-kDa interacting protein 3 (Bnip3) in normoxic coneΔVhl mice (Fig. 1d). This suggested that cells, presumably cones, activated a hypoxia-like response in retinas of coneΔVhl mice.

Fig. 1
figure 1

Knockdown of Vhl in cones of the all-cone mouse (coneΔVhl). a Expression of Cre recombinase in BPCre;R91W;Nrl-/-;ZsGreen mice. Green fluorescence (ZsGreen) indicates Cre activity. RPE: retinal pigment epithelium, ONL: outer nuclear layer, INL: inner nuclear layer, IPL: inner plexiform layer, GCL: ganglion cell layer. Scale bar 50 μm. b PCR of genomic DNA from retinas of 4-week-old ctrl and coneΔVhl mice. Floxed Vhl (fl) is detected at 460 bp and Cre-mediated excision (ex) results in a 260 bp fragment. c Western blot analysis of HIF1A in 4, 6, 8 and 12-week-old coneΔVhl and ctrl (littermates without Cre recombinase) mice. ACTB served as loading control. d Relative expression of hypoxic target genes in retinas of ctrl (black) and coneΔVhl (orange) mice. Expression levels were normalized to beta-actin (Actb) and calculated relatively to 4-week-old ctrl mice, which were set to 1. Shown are means ± SD. Two-way ANOVA with Šídák’s multiple comparison test was used for statistical analysis (Adm: ****p<0.0001, *p=0.0306; Egln1: ****p<0.0001; Glut1: ****p<0.0001; Bnip3: ****p<0.0001, **p=0.0043; n≥3 per time point). Adm: Adrenomedullin, Egln1: egl-9 family hypoxia-inducible factor 1, Glut1: Glucose transporter 1, Bnip3: BCL2/adenovirus E1B interacting protein 3. Cre-negative littermates were used as ctrl mice in all experiments unless otherwise indicated

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We observed an age-dependent decline in HIF1A protein levels and hypoxia target gene expression in coneΔVhl mice (Fig. 1c, d). This prompted us to analyze retinal function and morphology. B-wave amplitudes and photopic ERGs were not significantly different between coneΔVhl mice and ctrl mice at 6 weeks of age (Fig. 2a). However, the amplitudes were strongly reduced in 12-week-old coneΔVhl mice as compared to age-matched ctrl mice (Fig. 2b). This indicated an age-dependent loss of function potentially due to retinal degeneration. Indeed, we observed severe, progressive thinning of the outer nuclear layer (ONL) in coneΔVhl mice with most cones lost at 26 weeks of age (Fig. 3a, b). Additionally, partial loss of the retinal pigment epithelium (RPE) and strong perturbations in the inner nuclear layer (INL) were detected in older coneΔVhl mice (Fig. 3a, 12we coneΔVhl). In contrast, the ctrl mice showed only a slow age-related ONL thinning, as reported for the parental R91W;Nrl-/- mouse line before [32].

Fig. 2
figure 2

Reduced retinal function in aged coneΔVhl mice. a Averaged traces of single-flash photopic ERGs and b-wave amplitudes showed no significant differences in coneΔVhl mice (orange) compared to ctrl mice (black) at 6 weeks of age. b At 12 weeks of age, photopic b-wave amplitudes were strongly reduced in coneΔVhl mice (orange) as compared to ctrl mice (black). b-wave amplitudes are shown as means ± SD. Two-way ANOVA with Šídák’s multiple comparison test was used for statistical analysis (6we ctrl and coneΔVhl n=5, 12we ctrl n=6 and coneΔVhl n=4, *p=0.0115, ****p<0.0001)

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Fig. 3
figure 3

Progressive cone degeneration in coneΔVhl mice. a Inactivation of Vhl in cones led to progressive retinal degeneration (upper panels) as compared to ctrl mice (lower panels). ConeΔVhl mice displayed disturbed layering with reorganization of the INL (asterisk, 12we coneΔVhl), severe loss of the ONL and partial loss of the RPE (arrowhead, 12we coneΔVhl, inset) at 12 and 26 weeks of age. Abbreviations as in Fig. 1. Scale bar 50 μm. b Line chart of ONL thickness in coneΔVhl mice (orange) as compared to ctrl mice (black) at 4 and 12 weeks of age. n≥3. c Relative expression levels of genes involved in stress signaling and degeneration. Expression levels were normalized to Actb and calculated relatively to 4-week-old ctrl mice, which were set to 1. Shown are means ± SD. Two-way ANOVA with Šídák’s multiple comparison test was used for statistical analysis (Casp1: **p=0.0026, ****p<0.0001, *p=0.0244, Gfap: ****p<0.0001, **p=0.0035, ***p=0.0004, ****p<0.0001, Timp3: *p=0.0235, **p=0.0036, **p=0.0035, Vegf: ****p<0.0001, n≥3 per time point). Casp1: caspase 1, Gfap: glial fibrillary acidic protein, Timp3: tissue inhibitor of metalloproteinase 3, Vegf: vascular endothelial growth factor

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To gain insight into cellular mechanisms leading to cone degeneration, we analyzed expression of specific genes during aging in coneΔVhl and ctrl mice (Fig. 3c). Caspase 1 (Casp1), a gene involved in retinal degeneration, was upregulated in coneΔVhl mice at 8 weeks and peaked at 12 weeks of age. Expression of the stress signaling gene glial fibrillary acidic protein (Gfap) was upregulated in coneΔVhl mice already at 4 weeks and remained elevated throughout the observation period. Interestingly, we observed increased expression of tissue inhibitor of metalloproteinase 3 (Timp3) during the aging process, a gene associated with age-related macular degeneration [43, 44]. Similarly, expression of vascular endothelial growth factor (Vegf), a hypoxic response gene that is involved in neovascularization in wet AMD, was strongly increased in coneΔVhl mice at 4 and 6 weeks of age.

Subretinal neovascularization and vascular leakage in cone ΔVhl mice

Increased transcription of Timp3 is potentially associated with a higher risk to develop neovascular AMD [43] and hypoxia-regulated genes such as Vegf contribute to retinal and choroidal neovascularization (reviewed in [45]). Therefore, we analyzed the vascular network of coneΔVhl mice. All three vascular plexi were detected at 4 weeks of age (Fig. 4a, b, c). However, we observed vessels extending from the deep plexus into the normally avascular ONL in central retinas of coneΔVhl mice (Fig. 4a, arrowheads). Vessels reached the RPE but did not cross Bruch’s membrane (Fig. 4d, e), as we never observed retinal-choroidal anastomoses. This suggests that abnormal vessels originated from the retinal vasculature and not from the choroid. Interestingly, abnormal vessel growth was characteristic for the central but not for the peripheral retina (Fig. 4b). To evaluate the integrity of retinal vessels we performed fluorescein angiography. Signs of leakage were observed in coneΔVhl mice (Fig. 4f, g). To confirm these findings we stained retinal cross-sections for albumin (ALB), a marker for blood extravasation. Detailed analysis revealed strong ALB immunoreactivity in the ONL, RPE and inner plexiform layer (IPL) in coneΔVhl mice, as opposed to the signal detected in ctrl mice that was confined to retinal vessels. The choroid with its extensive vasculature was also strongly positive for ALB in both types of mice (Fig. 4h, i). Microglia/macrophages detected by IBA1 staining were found within the photoreceptor and subretinal layer in coneΔVhl but not ctrl mice (Fig. 4j, k).

Fig. 4
figure 4

Subretinal neovascularization in coneΔVhl mice. a-c Immunostaining of cross-sections of the central (a) and peripheral (b) retina of 4-week-old coneΔVhl and age-matched ctrl mice (c, central retina) with isolectin. Arrowheads indicate vessels extending from the deep plexus into the ONL in central retinas of coneΔVhl mice. DP: deep plexus, IP: intermediate plexus, PP: primary plexus. Scale bar 50 μm. d, e Light microscopy of 4-week-old coneΔVhl and ctrl mice. Neovessels (arrowheads) reached the RPE, but did not cross Bruch’s membrane. Scale bar 20 μm. f, g Fundus imaging and fluorescein angiography of coneΔVhl mice and ctrl mice at 4 weeks of age. Signs of leakage (arrowhead) were detected in coneΔVhl mice. h-k Retinal cross-sections of ctrl h, j and coneΔVhl mice (i, k) were stained with antibodies against ALB (h, i) and IBA1 (j, k) at 4 weeks of age. Microglia/macrophages were found within the photoreceptor layer and RPE in coneΔVhl mice (arrowheads). Scale bar 50 μm. Abbreviations as in Fig. 1

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Early vascular defects in cone ΔVhl mice

S-opsin expression starts shortly before birth in mice [46, 47] and we observed Cre-activity in BPCre;R91W;Nrl-/-;ZsGreen reporter mice as early as at postnatal day (PND) 1 (data not shown). The primary plexus in mice develops along a central-to-peripheral gradient and reaches the periphery around PND8-10 [48]. Vessels sprout from the primary plexus into the retina and turn laterally when they reach the outer and inner boundaries of the INL to first form the deep plexus and subsequently the intermediate plexus [48]. To determine the onset of neovascularization in coneΔVhl mice we stained retinal flat mounts with isolectin at PND7 and PND11. Using 3D-reconstruction of blood vessels, no difference in early vessel formation was detected between coneΔVhl and ctrl mice at PND7 (Fig. 5a). However, at PND11, before formation of the intermediate plexus, vessels growing from the deep plexus into the ONL were observed in coneΔVhl mice (Fig. 5b).

Fig. 5
figure 5

Pathological vessel growth starts around PND11 in coneΔVhl mice. a, b 3D-reconstruction of blood vessels stained with isolectin on retinal flat mounts of ctrl (left) and coneΔVhl (right) mice at PND7 (a) and PND 11 (b). At PND11, vessels extended from the deep plexus towards the ONL. The intermediate plexus has not yet been formed in both ctrl and coneΔVhl mice. For better recognition and distinction the z-value of the z-stacks was increased five times. DP: deep plexus, PP: primary plexus. Scale bar 50 μm. c Relative expression levels of angiogenic genes in retinas of coneΔVhl (orange), ctrl (black) and wt (green) mice at indicated time points. Expression levels were normalized to Actb and compared to ctrl mice at PND7, which were set to 1. Shown are means ± SD. Two-way ANOVA with Šídák’s multiple comparison test was used for statistical analysis comparing ctrl with coneΔVhl mice (Vegf: ****p<0.0001, Fgf2: ****p<0.0001, Pdgfb: ***p=0.0009, n=3 per time point). Vegf: vascular endothelial growth factor, Fgf2: fibroblast growth factor 2, Sema3f: semaphorin 3F, Pdgfb: platelet derived growth factor, B polypeptide, vWf: von Willebrand factor, Pdgfrb: platelet derived growth factor receptor, beta polypeptide

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Formation of the deep plexus is preceded by Vegf expression in the INL [49]. We analyzed gene expression levels in coneΔVhl, ctrl and 129S6 (wt, rod-dominant retina) mice to test for different regulation of genes involved in angiogenesis and vessel guidance during and after the process of vessel formation (PND7, 14 and 28, Fig. 5c). Vegf mRNA was not elevated at PND7, slightly increased by 1.3-fold at PND14 and significantly increased at PND28 (2-fold) in coneΔVhl mice compared to controls. Fibroblast growth factor 2 (Fgf2), a potent angiogenic factor that has been shown to be involved in retinal stress [50,51,52], was upregulated at PND28, at a time when developmental vascularization is completed. Recently, protective effects of semaphorin 3F (Sema3f) against subretinal neovascularization have been demonstrated [53]. We thus analyzed Sema3f gene expression levels to test for potential differential expression of this anti-angiogenic factor in coneΔVhl mice. However, expression of Sema3f in all-cone mice did not differ between ctrl and coneΔVhl mice (Fig. 5c). On the other hand, a pro-angiogenic factor, platelet derived growth factor, B polypeptide (Pdgfb), was upregulated in coneΔVhl compared to ctrl mice at PND28. Pdgfb expression by endothelial cells is essential for pericyte recruitment and is increased under hypoxia [54,55,56,57]. Surprisingly, expression levels of von Willebrand factor (vWf), a marker for endothelial cells, as well as of Pdgfb receptor (Pdgfrb), which is expressed by pericytes/mural cells [58,59,60,61], were not increased in coneΔVhl mice as compared to controls. The reason for this unexpected pattern of expression is not clear and requires further investigation.

HIF1 is responsible for pathological vessel growth and progressive cone degeneration in cone ΔVhl mice

We hypothesized that stabilized HIF1A, and not other Vhl targets, might promote retinal degeneration and neovascularization in coneΔVhl mice. Therefore, we additionally deleted Hif1a in cone photoreceptors (BPCre;R91W;Nrl-/-;Vhlf/f;Hif1af/f=coneΔVhlHif1a). After we confirmed the presence of deletion alleles for both Hif1a and Vhl in genomic DNA of coneΔVhlHif1a retinas (not shown) we determined retinal function. At 12 weeks of age, photopic ERG traces and b-wave amplitudes were similar in coneΔVhlHif1a and ctrl (R91W;Nrl-/-; Vhlf/f;Hif1af/f) mice (Fig. 6a, b) indicating no functional loss upon combined Hif1a and Vhl inactivation. Similarly, retinal morphology (Fig. 6c) and ONL thickness (Fig. 6d) of coneΔVhlHif1a mice was comparable to ctrl mice, whereas the thickness of the ONL in coneΔVhl mice was prominently reduced (same data as in Fig. 3b) at 12 weeks of age.

Fig. 6
figure 6

Combined deletion of Hif1a and Vhl (cone∆VhlHif1a) rescues the defects in retinal function, morphology and vasculature observed in cone∆Vhl mice. a, b Average single-flash photopic ERGs and b-wave amplitudes showed no significant differences in cone∆VhlHif1a mice (green) compared to ctrl mice (R91W;Nrl-/-;Vhlf/f; Hif1af/f, black) at 12 weeks of age. n=4 per group. b-wave amplitudes are shown as means ± SD. c, d Light microscopy analysis and measurement of the ONL thickness in cone∆VhlHif1a (green), ctrl mice (R91W;Nrl-/-;Vhlf/f; Hif1af/f, black) and coneΔVhl mice (orange, as shown in Fig. 3b) at indicated time points. Abbreviations as in Fig. 1. Scale bar 50 μm. e 3D-reconstruction of blood vessels stained with isolectin on retinal flat mounts of ctrl (left), coneΔVhl (middle) and cone∆VhlHif1a mice (right). Inactivation of Hif1a (cone∆VhlHif1a) rescued the pathological neovascularization. Analysis at 4 weeks of age, n=3. For better recognition and distinction the z-value of the z-stacks was increased five times. The yellow line indicates the border between the outer plexiform layer (OPL) and the ONL. DP: deep plexus, IP: intermediate plexus, PP: primary plexus. f Relative gene expression of hypoxic target genes in cone∆VhlHif1a mice (green) and ctrl mice (R91W;Nrl-/-;Vhlf/f; Hif1af/f, black) at 4we, 8we and 12we as indicated. Abbreviations as in Figs. 1 and 3. Expression levels were normalized to Actb and compared to 4-week-old ctrl mice, which were set to 1. Shown are means ± SD. n≥3 per time point. Scale bar 50 μm

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Combined deletion of Hif1a and Vhl also prevented pathological neovascularization into the ONL but had no effect on the developmental formation of the three vascular plexi (Fig. 6e). No induction of the expression of hypoxic target genes such as Adm, Egln1, Vegf and Timp3 was detected in coneΔVhlHif1a mice, confirming that HIF1 was the responsible HIF isoform for regulating their expression in cones (Fig. 6f). Altogether, these data demonstrate that combined deletion of Hif1a and Vhl (coneΔVhlHif1a mice) rescues the retinal phenotype observed in coneΔVhl mice and identify HIF1 as the causative factor for retinal degeneration and pathological vessel growth in coneΔVhl mice.

Discussion

Previously, it has been demonstrated that the knockdown of Vhl in rod cells causes HIF1A stabilization under normoxic conditions [39]. Here, we particularly investigated the effects of a chronic hypoxia-like response in cone photoreceptors. We show that ablation of Vhl in cones resulted in a chronic hypoxia-like situation with accumulation of HIF1A and induction of HIF1A target genes. This led to pathological vessel growth into the photoreceptor layer, reduced retinal function and severe, progressive cone degeneration. These consequences resemble some of the features of AMD pathology, particularly of a subset of neovascular AMD known as RAP. In human patients, HIF1A and HIF2A were detected in macrophages and endothelial cells of neovascular membranes associated with AMD [62]. Additionally, the correlation of drusen density and decreased choroidal blood flow [26] as well as choroidal ischemia in AMD patients [23,24,25] suggest reduced oxygen transport from the choroid to the inner retina [63]. Therefore, tissue hypoxia and HIFs might play an important role in disease development and/or progression.

Expression of numerous genes involved in metabolism, stress, cell survival and angiogenesis was increased in cone∆Vhl mice (Fig. 1d, 3c, 5c), as was Timp3 (Fig. 3c). TIMP3 is an important regulator of extracellular matrix (ECM) remodeling through inhibition of matrix metalloproteases and has been suggested to be a senescence-related protein [64] that is potentially regulated by HIF2 in rods [38]. Importantly, TIMP3 inhibits angiogenesis by interacting with VEGF [65] and is relevant for Bruch’s membrane integrity [64, 66]. Furthermore, mutations in Timp3 have been associated with Sorsby’s fundus dystrophy, an autosomal dominant maculopathy with submacular choroidal neovascularization [67, 68]. We can only speculate about the reasons for elevated Timp3 during the aging process in cone∆Vhl mice but it seems plausible that retinas of cone∆Vhl mice require extensive ECM remodeling due to progressive cone degeneration (Fig. 3).

We detected early and prominent vascular defects with abnormal vessel growth into the ONL, subretinal space and RPE layer in cone∆Vhl mice. Increased VEGF levels in transgenic mice expressing Vegf under the transcriptional control of the rhodopsin promoter (rho/VEGF mice) were shown to cause subretinal neovascularization [69,70,71]. In our model, Vegf was not upregulated at PND14, at a time when vessels had already started growing into the ONL (Fig. 5). However, transient Vegf expression in the INL regulates the formation of the deep plexus during development [49], i.e. already minor changes in the local Vegf concentration gradient, which are not detectable by RT-PCR, may misguide retinal vessels in cone∆Vhl mice. To date, it remains unclear which pro-angiogenic factor(s) are responsible for the observed neovascularization. Nonetheless, our findings demonstrate that this is a Hif1a-mediated mechanism, as additional deletion of Hif1a fully rescued the vascular defects (Fig. 6).

Interestingly, cone-specific deletion of Hif1a did not affect formation of the three vascular plexi (Fig. 6e), while it has been shown that knockdown of Hif1a in most cells of the retinal periphery prevented formation of the intermediate plexus [42]. Thus, our results suggest that Hif1a expression in cones, as opposed to other cells presumably in the INL, is not essential for the development of the intermediate plexus.

Our data do not define whether cone degeneration in cone∆Vhl mice is due to the effects of the chronic intrinsic activation of the hypoxic response or to early development of vascular defects. Another mouse model shows, however, that a chronic hypoxia-like response in rods leads to slow photoreceptor degeneration in the absence of reported vascular defects (rod∆Vhl mice [39]). This suggests that the long-term activation of HIF1 may reduce cell survival by an intrinsic mechanism. During hypoxia, HIF1 regulates mitochondrial respiration and thus metabolic adaptation towards glycolysis [72] leading to reduced energy (ATP) levels and potentially starvation. It has been shown that starvation of cones may lead to cone cell death [73]. Furthermore, it has been suggested that cones might be more sensitive to reduced oxygen and nutrient levels compared to rods, for reasons not fully understood [74, 75]. This could possibly explain the progressive degeneration and the accelerated phenotype in cone∆Vhl compared to rod∆Vhl mice.

It might also be possible, however, that photoreceptor degeneration is secondary to the early pathological vascular defects. Inactivation of Vhl in the retinal periphery during development leads to vessel growth into the ONL and severe retinal degeneration [52, 76]. Whereas the retinal periphery was strongly degenerated at 10 weeks of age, photoreceptor loss in the normal vascularized central retina was less pronounced [52]. We observed microglia activation and impaired function of the blood-retina-barrier in cone∆Vhl mice, as shown by IBA1 and ALB staining, respectively (Fig. 4h-k). It seems likely that retinal hemorrhages exacerbate cone photoreceptor degeneration in our model, as suggested for other models [77, 78]. Photoreceptor degeneration was also observed in very low density lipoprotein receptor knockout mice (Vldlr-/-; [79]) where early retinal neovascularization causes vessels to extend from the deep plexus into the subretinal space [80, 81]. Interestingly, it has been suggested that the Vldlr-/- phenotype is linked to HIF1A. Joyal and colleagues proposed that starved Vldlr-/- photoreceptors have reduced amounts of the Krebs cycle metabolite α-ketoglutarate, which decreases PHD activity and thus promotes stabilization of HIF1A. Consequently, Vegf is secreted, leading to RAP-like neovascularization [82]. We hypothesize that the combination of early vascular defects and the activation of a chronic hypoxia-like response, which affects expression of genes involved in cellular metabolism and retinal stress, leads to the progressive cone degeneration observed in coneΔVhl mice.

Our findings identify HIF1 in cones as the factor causing cone degeneration, pathological neovascularization and loss of function. Similarly, chronic activation of the hypoxic response in rods resulted in an HIF1- and age-dependent retinal degeneration (Barben et al., submitted). Whereas this provides strong evidence that activation of HIF1 in rod and cone photoreceptors leads to retinal degeneration, the activation of HIF2 but not of HIF1 is responsible for metabolic stress in RPE upon RPE-specific Vhl inactivation [75]. Thus, our data and data published by others suggest cell type-specific roles for HIF1A and HIF2A in retinal pathology.

Conclusions

Our data demonstrate that a chronic hypoxic response in cone photoreceptors induces HIF1A-mediated pathological vessel growth and cone degeneration. Evidence shows that HIF1A can be safely inactivated in cones (see above), rods ([38], Barben et al., submitted) and RPE [75] suggesting that targeting HIF transcription factors in photoreceptors and RPE may provide a potential therapeutic approach to rescue hypoxia-mediated retinal degeneration in patients and an alternative to anti-VEGF agents.

Abbreviations

Adm :

Adrenomedullin

ALB:

Albumin

AMD:

Age-related macular degeneration

Bnip3 :

BCL2/adenovirus E1B 19-kDa interacting protein 3

Casp1 :

Caspase 1

CNV:

Choroidal neovascularization

DAPI:

4',6-Diamidine-2'-phenylindole dihydrochloride

ECM:

Extracellular matrix

Egln1 :

Egl-9 family hypoxia-inducible factor 1 (known as Phd2)

ERG:

Electroretinography

Fgf2 :

Fibroblast growth factor 2

Gfap :

Glial fibrillary acidic protein

Glut1 :

Glucose transporter 1

HIF1A:

Hypoxia-inducible factor 1A

IBA1:

Alias, allograft inflammatory factor 1

INL:

Inner nuclear layer

IPL:

Inner plexiform layer

ONL:

Outer nuclear layer

Pdgfb :

Platelet derived growth factor, B polypeptide

Pdgfrb :

Pdgfb receptor

PFA:

Paraformaldehyde

PHD:

Prolyl hydroxylase domain

PND:

Postnatal day

RAP:

Retinal angiomatous proliferation

RPE:

Retinal pigment epithelium

RT:

Room temperature

Sema3f :

Semaphorin 3F

Timp3 :

Tissue inhibitor of metalloproteinase 3

VEGF:

Vascular endothelial growth factor

VHL:

Von Hippel Lindau protein

Vldlr :

Very low density lipoprotein receptor

vWf :

Von Willebrand factor

References

  1. Klein R, Klein BE, Cruickshanks KJ. The prevalence of age-related maculopathy by geographic region and ethnicity. Prog Retin Eye Res. 1999;18(3):371–89.

    Article  CAS  PubMed  Google Scholar 

  2. Buch H, Vinding T, La Cour M, Appleyard M, Jensen GB, Nielsen NV. Prevalence and causes of visual impairment and blindness among 9980 Scandinavian adults: the Copenhagen City Eye Study. Ophthalmology. 2004;111(1):53-61.

  3. Wong WL, Su X, Li X, Cheung CM, Klein R, Cheng CY, Wong TY. Global prevalence of age-related macular degeneration and disease burden projection for 2020 and 2040: a systematic review and meta-analysis. Lancet Glob Health. 2014;2(2):e106–16.

    Article  PubMed  Google Scholar 

  4. Green WR. Histopathology of age-related macular degeneration. Mol Vis. 1999;5:27.

    CAS  PubMed  Google Scholar 

  5. Swaroop A, Chew EY, Rickman CB, Abecasis GR. Unraveling a multifactorial late-onset disease: from genetic susceptibility to disease mechanisms for age-related macular degeneration. Annu Rev Genomics Hum Genet. 2009;10:19–43.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Spaide RF. Clinical Manifestations of Choroidal Neovascularization in AMD. In: Holz FG, Pauleikhoff D, Spaide RF, Bird AC, editors. Age-related Macular Degeneration. Berlin: Springer Berlin Heidelberg; 2013. p. 111–9.

    Chapter  Google Scholar 

  7. Rosenfeld PJ, Brown DM, Heier JS, Boyer DS, Kaiser PK, Chung CY, Kim RY, Group MS. Ranibizumab for neovascular age-related macular degeneration. N Engl J Med. 2006;355(14):1419–31.

    Article  CAS  PubMed  Google Scholar 

  8. Maguire MG, Martin DF, Ying GS, Jaffe GJ, Daniel E, Grunwald JE, Toth CA, Ferris FL 3rd, Fine SL. Five-Year Outcomes with Anti-Vascular Endothelial Growth Factor Treatment of Neovascular Age-Related Macular Degeneration: The Comparison of Age-Related Macular Degeneration Treatments Trials. Ophthalmology. 2016;123(8):1751–61.

    Article  PubMed  Google Scholar 

  9. Gragoudas ES, Adamis AP, Cunningham ET, Jr., Feinsod M, Guyer DR, Group VISiONCT. Pegaptanib for neovascular age-related macular degeneration. N Engl J Med. 2004;351(27):2805-2816.

  10. Bressler NM, Bressler SB, Fine SL. Age-related macular degeneration. Surv Ophthalmol. 1988;32(6):375–413.

    Article  CAS  PubMed  Google Scholar 

  11. Yannuzzi LA, Negrao S, Iida T, Carvalho C, Rodriguez-Coleman H, Slakter J, Freund KB, Sorenson J, Orlock D, Borodoker N. Retinal angiomatous proliferation in age-related macular degeneration. Retina. 2001;21(5):416–34.

    Article  CAS  PubMed  Google Scholar 

  12. Hartnett ME, Weiter JJ, Staurenghi G, Elsner AE. Deep retinal vascular anomalous complexes in advanced age-related macular degeneration. Ophthalmology. 1996;103(12):2042–53.

    Article  CAS  PubMed  Google Scholar 

  13. Yannuzzi LA, Freund KB, Takahashi BS. Review of retinal angiomatous proliferation or type 3 neovascularization. Retina. 2008;28(3):375–84.

    Article  PubMed  Google Scholar 

  14. Fritsche LG, Igl W, Bailey JN, Grassmann F, Sengupta S, Bragg-Gresham JL, Burdon KP, Hebbring SJ, Wen C, Gorski M, et al. A large genome-wide association study of age-related macular degeneration highlights contributions of rare and common variants. Nat Genet. 2016;48(2):134–43.

    Article  CAS  PubMed  Google Scholar 

  15. Chakravarthy U, Wong TY, Fletcher A, Piault E, Evans C, Zlateva G, Buggage R, Pleil A, Mitchell P. Clinical risk factors for age-related macular degeneration: a systematic review and meta-analysis. BMC Ophthalmol. 2010;10:31.

    Article  PubMed  PubMed Central  Google Scholar 

  16. Remsch H, Spraul CW, Lang GK, Lang GE. Changes of retinal capillary blood flow in age-related maculopathy. Graefes Arch Clin Exp Ophthalmol. 2000;238(12):960–4.

    Article  CAS  PubMed  Google Scholar 

  17. Arjamaa O, Nikinmaa M, Salminen A, Kaarniranta K. Regulatory role of HIF-1 a in the pathogenesis of age-related macular. Ageing Research Reviews. 2009;8:349–58.

    Article  CAS  PubMed  Google Scholar 

  18. Boltz A, Luksch A, Wimpissinger B, Maar N, Weigert G, Frantal S, Brannath W, Garhofer G, Ergun E, Stur M, Schmetterer L. Choroidal blood flow and progression of age-related macular degeneration in the fellow eye in patients with unilateral choroidal neovascularization. Invest Ophthalmol Vis Sci. 2010;51(8):4220–5.

    Article  PubMed  Google Scholar 

  19. Stefansson E, Geirsdottir A, Sigurdsson H. Metabolic physiology in age related macular degeneration. Prog Retin Eye Res. 2011;30(1):72–80.

    Article  CAS  PubMed  Google Scholar 

  20. Kent DL. Age-related macular degeneration: beyond anti-angiogenesis. Mol Vis. 2014;20:46–55.

    PubMed  PubMed Central  Google Scholar 

  21. Dallinger S, Findl O, Strenn K, Eichler HG, Wolzt M, Schmetterer L. Age dependence of choroidal blood flow. J Am Geriatr Soc. 1998;46(4):484–7.

    Article  CAS  PubMed  Google Scholar 

  22. Lam AK, Chan ST, Chan H, Chan B. The effect of age on ocular blood supply determined by pulsatile ocular blood flow and color Doppler ultrasonography. Optom Vis Sci. 2003;80(4):305–11.

    Article  PubMed  Google Scholar 

  23. Grunwald JE, Metelitsina TI, Dupont JC, Ying GS, Maguire MG. Reduced foveolar choroidal blood flow in eyes with increasing AMD severity. Invest Ophthalmol Vis Sci. 2005;46(3):1033–8.

    Article  PubMed  Google Scholar 

  24. Coleman DJ, Silverman RH, Rondeau MJ, Lloyd HO, Khanifar AA, Chan RV. Age-related macular degeneration: choroidal ischaemia. Br J Ophthalmol. 2013;97(8):1020–3.

    Article  PubMed  PubMed Central  Google Scholar 

  25. Ciulla TA, Harris A, Chung HS, Danis RP, Kagemann L, McNulty L, Pratt LM, Martin BJ. Color Doppler imaging discloses reduced ocular blood flow velocities in nonexudative age-related macular degeneration. Am J Ophthalmol. 1999;128(1):75–80.

    Article  CAS  PubMed  Google Scholar 

  26. Berenberg TL, Metelitsina TI, Madow B, Dai Y, Ying GS, Dupont JC, Grunwald L, Brucker AJ, Grunwald JE. The association between drusen extent and foveolar choroidal blood flow in age-related macular degeneration. Retina. 2012;32(1):25–31.

    Article  PubMed  PubMed Central  Google Scholar 

  27. Ames A 3rd, Li YY, Heher EC, Kimble CR. Energy metabolism of rabbit retina as related to function: high cost of Na+ transport. J Neurosci. 1992;12(3):840–53.

    CAS  PubMed  Google Scholar 

  28. Wang GL, Jiang BH, Rue EA, Semenza GL. Hypoxia-inducible factor 1 is a basic-helix-loop-helix-PAS heterodimer regulated by cellular O2 tension. Proc Natl Acad Sci U S A. 1995;92(12):5510–4.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  29. Jaakkola P, Mole DR, Tian YM, Wilson MI, Gielbert J, Gaskell SJ, von Kriegsheim A, Hebestreit HF, Mukherji M, Schofield CJ, et al. Targeting of HIF-alpha to the von Hippel-Lindau ubiquitylation complex by O2-regulated prolyl hydroxylation. Science. 2001;292(5516):468–72.

    Article  CAS  PubMed  Google Scholar 

  30. Semenza GL. Hydroxylation of HIF-1: oxygen sensing at the molecular level. Physiology (Bethesda). 2004;19:176–82.

    CAS  Google Scholar 

  31. Semenza GL. Oxygen sensing, homeostasis, and disease. N Engl J Med. 2011;365(6):537–47.

    Article  CAS  PubMed  Google Scholar 

  32. Samardzija M, Caprara C, Heynen SR, Willcox DeParis S, Meneau I, Traber G, Agca C, von Lintig J, Grimm C. A mouse model for studying cone photoreceptor pathologies. Invest Ophthalmol Vis Sci. 2014;55(8):5304–13.

    Article  CAS  PubMed  Google Scholar 

  33. Samardzija M, von Lintig J, Tanimoto N, Oberhauser V, Thiersch M, Reme CE, Seeliger M, Grimm C, Wenzel A. R91W mutation in Rpe65 leads to milder early-onset retinal dystrophy due to the generation of low levels of 11-cis-retinal. Hum Mol Genet. 2008;17(2):281–92.

    Article  CAS  PubMed  Google Scholar 

  34. Mears AJ, Kondo M, Swain PK, Takada Y, Bush RA, Saunders TL, Sieving PA, Swaroop A. Nrl is required for rod photoreceptor development. Nat Genet. 2001;29(4):447–52.

    Article  CAS  PubMed  Google Scholar 

  35. Haase VH, Glickman JN, Socolovsky M, Jaenisch R. Vascular tumors in livers with targeted inactivation of the von Hippel-Lindau tumor suppressor. Proc Natl Acad Sci U S A. 2001;98(4):1583–8.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  36. Akimoto M, Filippova E, Gage PJ, Zhu X, Craft CM, Swaroop A. Transgenic mice expressing Cre-recombinase specifically in M- or S-cone photoreceptors. Invest Ophthalmol Vis Sci. 2004;45(1):42–7.

    Article  PubMed  Google Scholar 

  37. Ryan HE, Poloni M, McNulty W, Elson D, Gassmann M, Arbeit JM, Johnson RS. Hypoxia-inducible factor-1alpha is a positive factor in solid tumor growth. Cancer Res. 2000;60(15):4010–5.

    CAS  PubMed  Google Scholar 

  38. Kast B, Schori C, Grimm C. Hypoxic preconditioning protects photoreceptors against light damage independently of hypoxia inducible transcription factors in rods. Exp Eye Res. 2016;146:60–71.

    Article  CAS  PubMed  Google Scholar 

  39. Lange C, Heynen SR, Tanimoto N, Thiersch M, Le YZ, Meneau I, Seeliger MW, Samardzija M, Caprara C, Grimm C. Normoxic activation of hypoxia-inducible factors in photoreceptors provides transient protection against light-induced retinal degeneration. Invest Ophthalmol Vis Sci. 2011;52(8):5872–80.

    Article  PubMed  Google Scholar 

  40. Madisen L, Zwingman TA, Sunkin SM, Oh SW, Zariwala HA, Gu H, Ng LL, Palmiter RD, Hawrylycz MJ, Jones AR, et al. A robust and high-throughput Cre reporting and characterization system for the whole mouse brain. Nat Neurosci. 2010;13(1):133–40.

    Article  CAS  PubMed  Google Scholar 

  41. Heynen SR, Tanimoto N, Joly S, Seeliger MW, Samardzija M, Grimm C. Retinal degeneration modulates intracellular localization of CDC42 in photoreceptors. Mol Vis. 2011;17:2934–46.

    CAS  PubMed  PubMed Central  Google Scholar 

  42. Caprara C, Thiersch M, Lange C, Joly S, Samardzija M, Grimm C. HIF1A is essential for the development of the intermediate plexus of the retinal vasculature. Invest Ophthalmol Vis Sci. 2011;52(5):2109–17.

    Article  CAS  PubMed  Google Scholar 

  43. Ardeljan D, Meyerle CB, Agron E, Wang JJ, Mitchell P, Chew EY, Zhao J, Maminishkis A, Chan CC, Tuo J. Influence of TIMP3/SYN3 polymorphisms on the phenotypic presentation of age-related macular degeneration. Eur J Hum Genet. 2013;21(10):1152–7.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  44. Vierkotten S, Muether PS, Fauser S. Overexpression of HTRA1 leads to ultrastructural changes in the elastic layer of Bruch's membrane via cleavage of extracellular matrix components. PLoS One. 2011;6(8):e22959.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  45. Campochiaro PA. Molecular pathogenesis of retinal and choroidal vascular diseases. Prog Retin Eye Res. 2015;49:67–81.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  46. Swaroop A, Kim D, Forrest D. Transcriptional regulation of photoreceptor development and homeostasis in the mammalian retina. Nat Rev Neurosci. 2010;11(8):563–76.

    Article  CAS  PubMed  Google Scholar 

  47. Roberts MR, Srinivas M, Forrest D, Morreale de Escobar G, Reh TA. Making the gradient: thyroid hormone regulates cone opsin expression in the developing mouse retina. Proc Natl Acad Sci U S A. 2006;103(16):6218–23.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  48. Fruttiger M. Development of the retinal vasculature. Angiogenesis. 2007;10(2):77–88.

    Article  PubMed  Google Scholar 

  49. Stone J, Itin A, Alon T, Pe'er J, Gnessin H, Chan-Ling T, Keshet E. Development of retinal vasculature is mediated by hypoxia-induced vascular endothelial growth factor (VEGF) expression by neuroglia. J Neurosci. 1995;15(7 Pt 1):4738–47.

    CAS  PubMed  Google Scholar 

  50. Baird A, Esch F, Gospodarowicz D, Guillemin R. Retina- and eye-derived endothelial cell growth factors: partial molecular characterization and identity with acidic and basic fibroblast growth factors. Biochemistry. 1985;24(27):7855–60.

    Article  CAS  PubMed  Google Scholar 

  51. Joly S, Lange C, Thiersch M, Samardzija M, Grimm C. Leukemia inhibitory factor extends the lifespan of injured photoreceptors in vivo. J Neurosci. 2008;28(51):13765–74.

    Article  CAS  PubMed  Google Scholar 

  52. Lange C, Caprara C, Tanimoto N, Beck S, Huber G, Samardzija M, Seeliger M, Grimm C. Retina-specific activation of a sustained hypoxia-like response leads to severe retinal degeneration and loss of vision. Neurobiol Dis. 2011;41(1):119–30.

    Article  CAS  PubMed  Google Scholar 

  53. Sun Y, Liegl R, Gong Y, Buhler A, Cakir B, Meng SS, Burnim SB, Liu CH, Reuer T, Zhang P, et al. Sema3f Protects Against Subretinal Neovascularization In Vivo. EBioMedicine. 2017;18:281–7.

    Article  PubMed  PubMed Central  Google Scholar 

  54. Lindblom P, Gerhardt H, Liebner S, Abramsson A, Enge M, Hellstrom M, Backstrom G, Fredriksson S, Landegren U, Nystrom HC, et al. Endothelial PDGF-B retention is required for proper investment of pericytes in the microvessel wall. Genes Dev. 2003;17(15):1835–40.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  55. Betsholtz C. Insight into the physiological functions of PDGF through genetic studies in mice. Cytokine Growth Factor Rev. 2004;15(4):215–28.

    Article  CAS  PubMed  Google Scholar 

  56. Lindahl P, Johansson BR, Leveen P, Betsholtz C. Pericyte loss and microaneurysm formation in PDGF-B-deficient mice. Science. 1997;277(5323):242–5.

    Article  CAS  PubMed  Google Scholar 

  57. Campochiaro PA. Ocular neovascularization. J Mol Med (Berl). 2013;91(3):311–21.

    Article  CAS  Google Scholar 

  58. Andrae J, Gallini R, Betsholtz C. Role of platelet-derived growth factors in physiology and medicine. Genes Dev. 2008;22(10):1276–312.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  59. Benjamin LE, Hemo I, Keshet EA. plasticity window for blood vessel remodelling is defined by pericyte coverage of the preformed endothelial network and is regulated by PDGF-B and VEGF. Development. 1998;125(9):1591–8.

    CAS  PubMed  Google Scholar 

  60. Fruttiger M. Development of the mouse retinal vasculature: angiogenesis versus vasculogenesis. Invest Ophthalmol Vis Sci. 2002;43(2):522–7.

    PubMed  Google Scholar 

  61. Hellstrom M, Kalen M, Lindahl P, Abramsson A, Betsholtz C. Role of PDGF-B and PDGFR-beta in recruitment of vascular smooth muscle cells and pericytes during embryonic blood vessel formation in the mouse. Development. 1999;126(14):3047–55.

    CAS  PubMed  Google Scholar 

  62. Inoue Y, Yanagi Y, Matsuura K, Takahashi H, Tamaki Y, Araie M. Expression of hypoxia-inducible factor 1alpha and 2alpha in choroidal neovascular membranes associated with age-related macular degeneration. Br J Ophthalmol. 2007;91(12):1720–1.

    Article  PubMed  PubMed Central  Google Scholar 

  63. Kim JH, Kim JR, Kang SW, Kim SJ, Ha HS. Thinner choroid and greater drusen extent in retinal angiomatous proliferation than in typical exudative age-related macular degeneration. Am J Ophthalmol. 2013;155(4):743-749, 749 e741-742.

  64. Kamei M, Hollyfield JG. TIMP-3 in Bruch's membrane: changes during aging and in age-related macular degeneration. Invest Ophthalmol Vis Sci. 1999;40(10):2367–75.

    CAS  PubMed  Google Scholar 

  65. Qi JH, Ebrahem Q, Moore N, Murphy G, Claesson-Welsh L, Bond M, Baker A, Anand-Apte B. A novel function for tissue inhibitor of metalloproteinases-3 (TIMP3): inhibition of angiogenesis by blockage of VEGF binding to VEGF receptor-2. Nat Med. 2003;9(4):407–15.

    Article  CAS  PubMed  Google Scholar 

  66. Macgregor AM, Eberhart CG, Fraig M, Lu J, Halushka MK. Tissue inhibitor of matrix metalloproteinase-3 levels in the extracellular matrix of lung, kidney, and eye increase with age. J Histochem Cytochem. 2009;57(3):207–13.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  67. Weber BH, Vogt G, Pruett RC, Stohr H, Felbor U. Mutations in the tissue inhibitor of metalloproteinases-3 (TIMP3) in patients with Sorsby's fundus dystrophy. Nat Genet. 1994;8(4):352–6.

    Article  CAS  PubMed  Google Scholar 

  68. Lin RJ, Blumenkranz MS, Binkley J, Wu K, Vollrath D. A novel His158Arg mutation in TIMP3 causes a late-onset form of Sorsby fundus dystrophy. Am J Ophthalmol. 2006;142(5):839–48.

    Article  CAS  PubMed  Google Scholar 

  69. Okamoto N, Tobe T, Hackett SF, Ozaki H, Vinores MA, LaRochelle W, Zack DJ, Campochiaro PA. Transgenic mice with increased expression of vascular endothelial growth factor in the retina: a new model of intraretinal and subretinal neovascularization. Am J Pathol. 1997;151(1):281–91.

    CAS  PubMed  PubMed Central  Google Scholar 

  70. Ohno-Matsui K, Hirose A, Yamamoto S, Saikia J, Okamoto N, Gehlbach P, Duh EJ, Hackett S, Chang M, Bok D, et al. Inducible expression of vascular endothelial growth factor in adult mice causes severe proliferative retinopathy and retinal detachment. Am J Pathol. 2002;160(2):711–9.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  71. Ida H, Tobe T, Nambu H, Matsumura M, Uyama M, Campochiaro PA. RPE cells modulate subretinal neovascularization, but do not cause regression in mice with sustained expression of VEGF. Invest Ophthalmol Vis Sci. 2003;44(12):5430–7.

    Article  PubMed  Google Scholar 

  72. Semenza GL. Oxygen-dependent regulation of mitochondrial respiration by hypoxia-inducible factor 1. Biochem J. 2007;405(1):1–9.

    Article  CAS  PubMed  Google Scholar 

  73. Punzo C, Kornacker K, Cepko CL. Stimulation of the insulin/mTOR pathway delays cone death in a mouse model of retinitis pigmentosa. Nat Neurosci. 2009;12(1):44–52.

    Article  CAS  PubMed  Google Scholar 

  74. Punzo C, Xiong W, Cepko CL. Loss of daylight vision in retinal degeneration: are oxidative stress and metabolic dysregulation to blame? J Biol Chem. 2012;287(3):1642–8.

    Article  CAS  PubMed  Google Scholar 

  75. Kurihara T, Westenskow PD, Gantner ML, Usui Y, Schultz A, Bravo S, Aguilar E, Wittgrove C, Friedlander MS, Paris LP, et al. Hypoxia-induced metabolic stress in retinal pigment epithelial cells is sufficient to induce photoreceptor degeneration. Elife. 2016;5:e14319.

    Article  PubMed  PubMed Central  Google Scholar 

  76. Kurihara T, Kubota Y, Ozawa Y, Takubo K, Noda K, Simon MC, Johnson RS, Suematsu M, Tsubota K, Ishida S, et al. von Hippel-Lindau protein regulates transition from the fetal to the adult circulatory system in retina. Development. 2010;137(9):1563–71.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  77. Zhao L, Ma W, Fariss RN, Wong WT. Minocycline attenuates photoreceptor degeneration in a mouse model of subretinal hemorrhage. microglial: inhibition as a potential therapeutic strategy. Am J Pathol. 2011;179(3):1265–77.

    CAS  PubMed  Google Scholar 

  78. Geiger P, Barben M, Grimm C, Samardzija M. Blue light-induced retinal lesions, intraretinal vascular leakage and edema formation in the all-cone mouse retina. Cell Death Dis. 2015;6:e1985.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  79. Frykman PK, Brown MS, Yamamoto T, Goldstein JL, Herz J. Normal plasma lipoproteins and fertility in gene-targeted mice homozygous for a disruption in the gene encoding very low density lipoprotein receptor. Proc Natl Acad Sci U S A. 1995;92(18):8453–7.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  80. Heckenlively JR, Hawes NL, Friedlander M, Nusinowitz S, Hurd R, Davisson M, Chang B. Mouse model of subretinal neovascularization with choroidal anastomosis. Retina. 2003;23(4):518–22.

    Article  PubMed  Google Scholar 

  81. Hu W, Jiang A, Liang J, Meng H, Chang B, Gao H, Qiao X. Expression of VLDLR in the retina and evolution of subretinal neovascularization in the knockout mouse model's retinal angiomatous proliferation. Invest Ophthalmol Vis Sci. 2008;49(1):407–15.

    Article  PubMed  Google Scholar 

  82. Joyal JS, Sun Y, Gantner ML, Shao Z, Evans LP, Saba N, Fredrick T, Burnim S, Kim JS, Patel G, et al. Retinal lipid and glucose metabolism dictates angiogenesis through the lipid sensor Ffar1. Nat Med. 2016;22(4):439–45.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

The authors thank Andrea Gubler, Cornelia Imsand and Christel Beck for their excellent technical support.

Funding

Supported by the Swiss National Science Foundation (SNF #31003A_149311 and 31003A_173008).

Availability of data and materials

All data generated or analyzed during this study are included in this published article or available from the corresponding author on reasonable request.

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    Authors and Affiliations

    1. Laboratory for Retinal Cell Biology, Department of Ophthalmology, University Hospital Zurich, University of Zurich, Zurich, Switzerland

      Maya Barben, Christian Schori, Marijana Samardzija & Christian Grimm

    2. Neuroscience Center Zurich (ZNZ), University of Zurich, Zurich, Switzerland

      Maya Barben & Christian Grimm

    3. Zurich Center for Integrative Human Physiology (ZIHP), University of Zurich, Zurich, Switzerland

      Christian Schori & Christian Grimm

    Authors
    1. Maya Barben
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    2. Christian Schori
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    3. Marijana Samardzija
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    4. Christian Grimm
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    Contributions

    MB, MS, CG designed research; MB, MS, CS performed research; MB, MS, CG analyzed data, MB, MS, CG wrote the paper. All authors read and approved the final manuscript.

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    Correspondence to Marijana Samardzija.

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    Ethics approval and consent to participate

    All experimental procedures were performed according to ‘The Association for Research in Vision and Ophthalmology’ statement on animal use in ophthalmic and vision research and the regulation of the veterinary authorities of Kanton Zurich, Switzerland. Animal protocols were approved by the veterinary authorities of Kanton Zurich, Switzerland.

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    Cite this article

    Barben, M., Schori, C., Samardzija, M. et al. Targeting Hif1a rescues cone degeneration and prevents subretinal neovascularization in a model of chronic hypoxia. Mol Neurodegeneration 13, 12 (2018). https://doi.org/10.1186/s13024-018-0243-y

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    • DOI: https://doi.org/10.1186/s13024-018-0243-y

    Keywords

    Molecular Neurodegeneration

    ISSN: 1750-1326