- Research article
- Open Access
Intracellular amyloid formation in muscle cells of Aβ-transgenic Caenorhabditis elegans: determinants and physiological role in copper detoxification
© Minniti et al; licensee BioMed Central Ltd. 2009
Received: 04 September 2008
Accepted: 06 January 2009
Published: 06 January 2009
The amyloid β-peptide is a ubiquitous peptide, which is prone to aggregate forming soluble toxic oligomers and insoluble less-toxic aggregates. The intrinsic and external/environmental factors that determine Aβ aggregation in vivo are poorly understood, as well as the cellular meaning of this process itself. Genetic data as well as cell biological and biochemical evidence strongly support the hypothesis that Aβ is a major player in the onset and development of Alzheimer's disease. In addition, it is also known that Aβ is involved in Inclusion Body Myositis, a common myopathy of the elderly in which the peptide accumulates intracellularly.
In the present work, we found that intracellular Aβ aggregation in muscle cells of Caenorhabditis elegans overexpressing Aβ peptide is affected by two single amino acid substitutions, E22G (Arctic) and V18A (NIC). Both variations show decrease intracellular amyloidogenesis compared to wild type Aβ. We show that intracellular amyloid aggregation of wild type Aβ is accelerated by Cu2+ and diminished by copper chelators. Moreover, we demonstrate through toxicity and behavioral assays that Aβ-transgenic worms display a higher tolerance to Cu2+ toxic effects and that this resistance may be linked to the formation of amyloid aggregates.
Our data show that intracellular Aβ amyloid aggregates may trap excess of free Cu2+ buffering its cytotoxic effects and that accelerated intracellular Aβ aggregation may be part of a cell protective mechanism.
Aberrant protein aggregation is a common feature of late-onset amyloidogenic diseases, such as Alzheimer's disease (AD) and Inclusion Body Myositis (IBM) . A peptide of 39–43 mer derived from the amyloid precursor protein (APP), the amyloid β-peptide (Aβ), is the main constituent of senile plaques (SPs) in AD , and it is also one of the hallmarks of IBM, a common myopathy characterized by the presence of intracellular amyloid aggregates in skeletal muscle cells [1, 3]. IBM patients show progressive muscle weakness, compromised muscle innervation  and muscle fibre degeneration . Besides the presence of senile plaque-like inclusions, most molecules known to be involved in IBM are also present in AD .
Dominant mutations in the APP gene are associated with rare cases of familial AD, in which brain and vascular amyloid deposits are formed earlier than in sporadic cases . One type of familial AD is linked to a point mutation in the Aβ peptide (E22G, Arctic) that accelerates aggregation of Aβ into protofibrils and fibrils in vitro [8–12]. Some transgenic mice carrying the Arctic mutation show increased plaque formation but normal learning and memory compared to strains carrying the wild type Aβ . Opposite to the Arctic variant, in vitro studies demonstrated that the NIC mutation (V18A), which is predicted to favour the α helix conformation over the β sheet conformation, shows decreased aggregation .
Although APP and Aβ have been implicated in several processes in vitro and in vivo, such as neuronal development and cell survival, the in vivo functions of APP and Aβ remain unclear [7, 15, 16]. Moreover, the inactivation of the complete APP gene family has shown that APP is necessary for neurodevelopment and cell adhesion . In addition, neither the in vivo mechanisms of amyloid formation nor the factors required for this process have been properly established. It is also unknown if the formation of insoluble amyloid aggregates is instrumental for the onset of these amyloidogenic diseases, or if the formation of amyloid structures is the end product of the cell protective machinery. SPs are structured as metal-enriched aggregates that accumulate Cu2+, Fe3+, and Zn2+ [18–20]. In agreement with this, the aggregation state of Aβ-peptide is increased by Cu2+ or Zn2+  and reduced by metal chelators such as clioquinol [22–24]. However, the role of copper as deleterious or beneficial in the context of amyloidogenic diseases is very controversial. For instance, in a transgenic APP23 mouse model, supplementation of the diet with bioavailable copper restored normal levels of SOD-1 activity and decreased the production of soluble Aβ . From these studies, the structural plasticity of the SP is becoming evident in agreement with previous structural data . In light of this evidence we postulate that the formation of the SP might be triggered by fluctuations on the levels of transition metals, and could be a part of a protective homeostatic mechanism gone off-course. However, it is unknown if these metals are accumulated in the intracellular amyloid aggregates observed in IBM and if the aggregation state of Aβ is modulated by transition metals in this intracellular muscle environment. Interestingly, IBM patients do not develop dementia and AD patients do not have the muscle weakness characteristic of IBM, which indicates that these diseases may be triggered by independent mechanisms .
Caenorhabditis elegans has an APP homologue but this protein lacks a region equivalent to the Aβ-peptide . However, several studies carried out in Aβ overexpressing C. elegans  have established that the formation of amyloid deposits  could induce oxidative stress , stress response [30, 31] and up- or down-regulation of different genes . However, the toxic Aβ-species responsible for these effects detected in Aβ-transgenic worms have not been thoroughly identified, but they are likely to be a specific type of Aβ oligomers rather than mature Aβ amyloid aggregates [33–35]. It is important to mention that the paralysis phenotype is only observed in Aβ expressing C. elegans in a dominant rol-6 background (strains carrying a mutation in a collagen gene) . In fact, the great majority of IBM patients show muscle weakness that translates in a lower quality of life but not paralysis or decreased longevity .
In the present work, we evaluated intrinsic and extrinsic factors on the aggregation of intracellular Aβ peptides constitutively overexpressed in muscle cells of C. elegans. Our results indicate that intracellular Aβ aggregation is selectively affected by single amino acid substitution, which is in agreement with previously published in vitro data . Under our experimental conditions, we find that the NIC mutation (V18A) did form fewer Thioflavine-S (ThS)-positive aggregates compared to Aβ wt. Neither non-aggregating Aβ peptide nor aggregating Aβ peptide induced strong paralysis phenotypes in these animals in a wild type background. However, Aβ wild type affected the worms' motility as they aged mimicking the muscle weakness observed in IBM patients. Therefore, we asked the question whether CuCl2 could modulate the aggregation of intracellular Aβ-peptide in muscle cells, as it is apparent with the extracellular Aβ-peptide in transgenic vertebrate models for AD [22, 37]. We found that Cu2+ increases the number of intracellular ThS-positive aggregates present in this model. In contrast, the presence of the Cu-chelators histidine and clioquinol in the growing medium decreases the formation of amyloid deposits. Surprisingly, the Aβ-expressing animals, in spite of producing a great number of amyloid aggregates in the presence of Cu2+, exhibited increased tolerance to the cytotoxic effects of CuCl2 compared to control animals. Therefore, the present evidence suggests, for the first time, that intracellular amyloid deposits are dynamically structured by the presence of metals in muscle cells, and that the formation of intracellular Aβ aggregates could be a part of the homeostatic mechanism responsible for protecting cells against abrupt changes in Cu2+ levels.
The NIC Aβ1–42 variant displays a low rate formation of intracellular amyloid deposits in C. elegansmuscle cells
Cu2+ modulates intracellular Aβ amyloidosis in transgenic C. elegans
Cu2+ tolerance in Aβ- transgenic C. elegans
Copper incorporation into Aβ expressing C. elegans and controls
No copper added
CuCl2 150 μM
0.350 ± 0.458
0.169 ± 0.142
12.109 ± 2.523
0.128 ± 0.110
9.931 ± 0.930
0.068 ± 0.015
9.224 ± 1.989
In order to confirm that the protective effect of Aβ expression is limited to copper toxicity we performed CdCl2 and ZnCl2 toxicity assays. Both metals are known to be toxic and to elicit a stress response in C. elegans [43–45]. Furthermore, zinc is known to be involved in the metallobiology of Alzheimer's disease . We found that the survival curves (Fig. 5C, D) are almost identical between Aβ expressing worms and controls in both cases. The differences in LC50 for cadmium (CL2120: 1.55 ± 0.02 mM; CL2122: 1.59 ± 0.03 mM) and zinc (CL2120: 4.9 ± 0.8 mM; CL2122: 4.8 ± 0.4 mM) are not statistically significant between the strains. These results exclude the possibility that Aβ overexpression may be providing a more general protective effect, at least to metal toxicity.
Finally, to correlate amyloid aggregate load with resistance to copper toxicity, we performed lifespan experiments to determine if Aβ expressing worms show longer lifespan than controls under 150 μM copper. We found that Aβ worms show a small but significant increase in median and total lifespan (Fig. 5B) demonstrating the protective effect of Aβ when the worms were chronically exposed to copper. When cultured on regular agar, the Aβ expressing worms and the control animals show a similar lifespan (Additional file 1).
Aβ- transgenic C. elegans are protected from Cu2+toxicity in behavioral assays
Most of the studies performed to understand intracellular Aβ aggregation and amyloid formation have been done using in vitro systems. Therefore, neither the in vivo mechanisms of amyloid formation nor the factors required for this process have been established . In the present work, we evaluated intrinsic and extrinsic factors in the aggregation of Aβ peptides constitutively overexpressed in muscle cells of Caenorhabditis elegans. Under our experimental conditions, we found that Aβ peptide carrying the Arctic (E22G) or the NIC mutation (V18A) did form few ThS-positive aggregates compared to Aβwt. The results obtained with the NIC (V18A) variant are in agreement with previously published in vitro data . This mutation is expected to favor the α helix over the β sheet conformation, which would cause a decrease in amyloidosis . However, the results obtained with the Arctic mutation (E22G) are at variance with the literature . The initial delay in the formation of aggregates is in agreement with the kinetics of aggregation of the Arctic variant in vitro in which there is a lag in the formation of amyloid in favor of the development of protofibrils, when compared with the Aβwt . However, the poor amyloidogenesis in later time points (even lower than the NIC variant) is probably due to low transgene expression since the Arctic variant is known to be highly amyloidogenic as established in studies of transgenic mice expressing this human variant in the nervous system [13, 50]. Our inability to produce transgenic lines that constitutively express high levels of Aβ Arctic could be related to the deleterious effect of high levels of this variant during embryonic stages that may have preclude us from obtaining such strains. On the other hand, the immunofluorescence experiments show that much of the Aβ Arctic is aggregated in what appears to be amorphous deposits. Perhaps, there are muscle expressed factors that prevent the formation of mature amyloid deposits of the Aβ Arctic variant.
In our model, Aβwt peptide expression induced a clear decrease in the worms' motility (Fig. 6), which could be compared to the progressive muscle weakness present in IBM patients. The lack of muscle degeneration observed in the Aβwt expressing animals suggests that the Aβ peptide might not be sufficient to induce a stronger myopathy by itself in the muscle cells of worms, and that extra factors might be needed to fully replicate IBM symptoms in C. elegans. The situation could be very similar to that seen in triple transgenic mice models for AD, where mice need to overexpress Aβ together with Tau and presenilin (all mutant variants) to replicate the brain human features characteristic of the pathology . Therefore, Aβ apparently needs to act with other factor/s present in the muscle to fully induce IBM symptoms in worm muscle .
We also showed that Cu2+ and the Cu2+ chelators histidine and clioquinol can modulate the formation of intracellular amyloid aggregates in C. elegans muscle cells. Copper ionophores like clioquinol and PBT2 show benefits in transgenic mouse models and phase 2 clinical trials of AD [22, 24, 52]. Despite the differences in cellular location, intracellular Aβ aggregation in C. elegans seems to be enhanced by Cu2+ and diminished by histidine and clioquinol in accordance with previous in vitro and in vivo reports [22, 23]. The increased amyloidosis could also be the result of higher Aβ expression brought about by Cu exposure and lower Aβ expression caused by Cu-chelator treatment. This situation is not likely to be the case because the constitutive unc-54 promoter is driving Aβ expression. Moreover, it has been shown that in addition to aggregated Aβ-peptide there is an important pool of soluble peptide in worms expressing Aβ . Therefore, increased peptide synthesis is not necessary for an eventual increment in Aβ for interaction and subsequent aggregation in the presence of Cu2+. We did, however, evaluate Aβ expression in worms subjected to the different treatments by semi-quantitative RT-PCR and found that there were no significant differences in its expression (data not shown). Therefore, these results support a pivotal role of Cu2+ in the aggregation state of intracellular Aβ.
In the present study, the animals were treated continuously with CuCl2, histidine or clioquinol since they were embryos; at this stage there is no Aβ aggregation that can be observed by ThS staining although some aggregates have been detected with the X-34 dye . Therefore, we have yet to establish whether treating animals with histidine or clioquinol later in life, once a significant number of deposits have been formed, can eliminate or reduce the number and/or size of the aggregates. Although we have shown an effect of Cu2+, histidine and clioquinol on the formation of intracellular amyloid deposits in muscle cells by histochemical staining with ThS, we have not yet resolved the question of the relative concentrations of soluble versus insoluble aggregated Aβ and their changes upon treatment with Cu-chelators. It would be very informative to clarify this point by double staining with ThS or X-34 to visualize the deposits, and Aβ antibodies that recognize both soluble and insoluble forms of the amyloid peptide. This experiment would clarify whether Cu2+ and Cu-chelators influence the equilibrium between both Aβ forms.
The differences observed among individuals exposed to the same treatments could be due to the developmental stage at which the animals start their exposure to the different agents. Even though we start the treatments on embryos, there is some difference in the actual developmental stage of the embryo (data not shown). Although this is a possibility, we do not think it is the case, since we have observed similar differences in animals cultured on standard medium. It is unlikely that the differences observed between same age individuals are due to genetic background. Self-fertilizing reproduction in C. elegans precludes much genetic variation in the strains. The strains used in our studies are transgenic strains in which several copies of Aβ had been integrated into the genome. However, integration of a transgene into the genome does not always prevent mosaicism and/or differences in expression between individuals .
Another caveat is that we do not know if there is enrichment of metals (Cu, Zn and others) in the intracellular amyloid deposits of C. elegans muscle cells as is the case in the extracellular amyloid aggregates found in AD affected neuronal tissue . The determination of metal levels in the muscle aggregates would be an important step in drawing a firmer analogy between AD extracellular brain aggregates and intracellular muscle deposits.
Perhaps the most striking finding is that the Aβ-expressing animals, in spite of producing great number of amyloid aggregates in the presence of Cu2+, exhibited increased tolerance to the cytotoxic effects of CuCl2 compared to control animals and suggests that there might be less metal available to produce cell damage in this strain. The results from the behavioural experiments together with the results obtained in the toxicity assays offer strong evidence that Aβ may be providing protection to Cu2+ toxicity. Interestingly, the Aβ expressing worms that were exposed to CuCl2 move better than those that were not cultured in the presence of this transition metal, suggesting that perhaps the increase in amyloid deposits in these treated worms is limiting the amount of the more toxic oligomeric species [33–35]. Our findings connect with those of other authors showing that increased Aβ deposition associates with decrease levels of neuronal oxidative damage in Down Syndrome and AD [54–57]. It has also been suggested that the chelation of redox-active copper and iron may be the most important mechanism by which Aβ exerts its protective function , and that at moderate copper concentrations, Aβ acts as an antioxidant to prevent Cu2+ catalyzed oxidation of biomolecules . The levels of intracellular copper must be strictly regulated to prevent aberrant reactive oxygen species generation resulting in neuronal and muscle damage. In fact, intracellular free copper has been estimated to be almost nil , and is under the control of a robust metalloprotein array that rapidly and efficiently buffers free copper bioavailability. However, this protein array is clearly altered in age-related diseases such as Alzheimer's disease , which may explain why copper is accumulated in amyloid plaques of people suffering of this disease . Moreover, a more recent report described that total brain copper levels increase in aged mouse . Considering that age is a risk factor for AD neuropathology, brain copper rise in elderly humans may be one of the neurochemical factors relevant in the onset of AD. Moreover, cellular acidosis could facilitate the release of copper from metal binding proteins . Interestingly, brain acidosis is observed in AD , which may facilitate the release of copper from copper-binding proteins increasing the formation of Aβ-Cu aggregates. This may be also part of the mechanism that increases copper bioavailability in IBM.
Therefore, the present evidence suggests that intracellular amyloid deposits are dynamically structured by metals, and that Aβ may be a component of the system that specifically controls copper homeostasis at the cellular level [65–67].
Much research has been done on the possible role of copper and other metals such as zinc and iron on the development of AD, but so far no consensus has been reached in terms of the specific involvement of copper in this pathology, and conflicting reports point either to a deleterious role of copper  or a beneficial one . Moreover, there is practically no data available about the possible role of metal homeostasis on other disorders characterized by amyloidosis such as sporadic IBM.
In this study we show in a C. elegans model that the intracellular senile plaque-like structures formed in muscle cells and characteristic of IBM can be modulated by copper and copper chelators. The accelerated Aβ aggregation in the presence of copper seems to be linked to a higher tolerance to the cytotoxic effects of this metal. Our results suggest that the formation of intracellular Aβ aggregates could be part of a mechanism that defends the cell against sudden changes in copper concentration.
Distilled H2O (dH2O) used in buffer preparations was submitted to resin treatment in order to eliminate organic compounds, cations and anions. After treatment, the resultant dH2O had a resistance value of 18 MΩ/cm.
Nematode propagation and strains
Transgenic strains CL2120 and CL2122 were a gift of Dr. Christopher Link and were described earlier . Briefly, CL2120 expresses the Aβ1–42 peptide under the control of the unc-54 (myosin heavy chain) promoter that drives expression to the body wall muscle cells. These animals form intracellular amyloid deposits constitutively in their muscle cells . CL2122 has the same vector as CL2120, minus Aβ1–42, incorporated into the genome and therefore it does not form amyloid aggregates. Both strains have mtl-2/GFP as marker gene and therefore they express GFP in the intestinal cells. The worms were cultured on regular 35, 60 or 100 mm culture plates with NGM agar seeded with the bacterial strain OP50 . The agar and the bacterial cultures were supplemented with histidine (Sigma), clioquinol (Sigma) and CuCl2 (Sigma) at a concentration of 150 μM. All strains were maintained at 20°C. Worm cultures were synchronized by the hypochlorite method  and the resulting eggs were seeded onto the previously described agar plates. The animals were collected every 24 h by washing the plates with M9 buffer . The worms were precipitated in a clinical centrifuge and the M9 removed. The nematodes were then stained as described below.
Construction of the transgenic strains expressing the Aβ variants, Arctic (E22G) and NIC (V18A) (Fig. 1A), were obtained by site directed mutagenesis on the pCL12 plasmid (kindly provided by Dr. Christopher Link) that carries the human wt Aβ1–42 peptide (with a signal peptide in the N-terminus) under the control of the unc-54 promoter that drives expression to the body wall muscle cells . The NIC mutation (V18A) induces a significant increment in the α-helical content of Aβ and dramatically diminishes fibrillogenesis in vitro . The primers used to generate the Arctic (5'TGGTGTTCTTTGCAGGAGATGTGGGTTCAAA3' and its complementary anti-sense primer) and NIC (5'ATCATCAAAAATTGGCGTTCTTTGCAGAAGA3' and its complementary anti-sense primer) mutations were obtained commercially (Invitrogen). We obtained plasmids pPG110 and pPG120 carrying the Arctic and the NIC variant respectively as established by sequencing the DNA inserts. The transgenic lines were created using microparticle bombardment of the plasmid of interest and the selectable co-transformation marker unc-119 (pJN254) into unc-119 (e2498) mutants, as described earlier except that we used tungsten particles instead of gold particles . The presence of each transgene was determined by PCR (primer sense 5'TGACCGTGGAAGATTA 3' located in the unc-54 promoter and primer antisense 5'GTCCAATGATTGCACCT 3' between bases 1208 and 1224 of the pCL12 plasmid and its derivatives pPG110 and pPG120 (Fig. 1B). The expression of the transgenes was established by western blot analysis (Fig. 1C and 1D) and immunofluorescence (6E10 antibody, Sigma) (Fig. 3). We obtained 4 transgenic lines that express the NIC variant and 10 expressing the Arctic variant. For Western analysis we choose lines in which the transgene was incorporated into the genome and therefore transferred to 100% of the progeny. For the analysis of intracellular aggregation (ThS and immunofluorescence) we choose lines with equivalent Aβ expression (NIC2). This was not possible for the Arctic variant since it we could not obtain strains expressing this variant at high levels, but we nevertheless analysed strain Arctic 6.
Western blot analysis
Worms were collected form the plates with M9 buffer, transferred to tubes, centrifuged and washed two times to eliminate bacteria. The final worm pellet was resuspended in 100 μl lysis buffer (50 mM HEPES pH = 7.5; 6 mM MgCl2; 1 mM EDTA; 75 mM sucrose; 25 mM benzamidine; 1 mM DTT; 1% Triton X-100) and frozen at 80°C. The samples were sonicated 2 times on ice for 15 seconds and centrifuged 5 minutes at 10,000 rpm to eliminate the cuticles. The supernatant was transferred to a new tube and total protein content was quantified. Then, a volume corresponding to 50 μg protein was precipitated with acetone. The pellet was resuspended in loading buffer (50 mM Tris-HCl pH6.8; 100 mM DTT; 5% SDS; 10 % glycerol; bromophenol blue; 5% β-mercaptoethanol) and boiled for 3 minutes (adapted from Wu et al. 2006). The samples were subjected to electrophoresis on Tris-Tricine polyacrilamide gels at constant voltage (100 V) at 4°C. The proteins were then transferred to PVDF membranes for 2 hours at 4°C (300 mA). The membranes were boiled in PBS for 3 minutes before blocking with a solution of PBT plus 5% milk for one hour at room temperature. We used the anti-Aβ monoclonal antibody 6E10 (Chemicon) at 1:1,000 dilution or anti-α-tubulin monoclonal antibody (Sigma) at 1:5,000 dilution overnight at 4°C. We used goat anti-mouse HRP as secondary antibody. The densitometry analysis was performed with Matrix software.
We used the monoclonal anti-Aβ antibody 6E10 (Sigma) and as secondary antibody an anti-mouse Alexa Fluor 488 (Molecular Probes) pre-adsorbed with fixed worms. The fixation, permeabilization, and staining of the specimens were performed as described before . Some samples were also stained with rhodamine-phalloidin (1:4,000) for the visualization of actin filaments in muscle cells.
ThS fluorescence staining
ThS staining was performed as described before  with the modification that it was done in whole C. elegans populations instead of on individual worms. Briefly, the worms washed from the plates were submitted to a brief centrifugation at 3,000 rpm in microfuge tubes and fixed by adding 1 ml 4% paraformaldehyde in PBS pH 7.4 for 24 h at 4°C. The next day, the fixative was removed, replaced by 0.5 ml permeabilization solution (1% Triton X-100, 5% β-mercaptoethanol, 125 mM Tris, pH 7.4), and incubated at 37°C for 24 h. After removing the permeabilization solution the animals were washed in PBS-T (Triton X-100 0.1%) pH 7.4, stained in 0.125% ThS in 50% ethanol for 2 min and distained for another 2 min in 50% ethanol. The 50% ethanol was removed; the worm pellet was resuspended in a drop of PBS and a small aliquot observed under a Zeiss Axioplan microscope. If the background of the staining was too high a second wash with 50% ethanol was performed. The animals were finally mounted on a drop of mounting medium (DAKO), covered with a cover slip and sealed. The samples were coded, and the counting and analysis was performed double blind. The samples were then observed and photographed with a Zeiss Axioplan microscope equipped with epifluorescence or analysed in a Zeiss LSM 2000 MT confocal microscope. To quantify the amyloid aggregates, the ThS stained deposits present anterior to the pharyngeal bulb were counted in a minimum of 40 worms in three independent experiments.
Image quantification and statistical analysis
Digital quantification of areas positive for ThS fluorescence were measured with the Sigma Scan Pro 5 or ImageJ. The analysis revealed the total average area of the deposits. The total area of deposits was divided by the total head area to standardize it to the worm size. Data from the image analysis microscopy were exported to a Sigma Plot file or GraphPad Prism 4 for statistical analysis. Results were expressed as mean ± standard error. Statistical significance was determined by one-way analysis of variance (ANOVA); p < 0.05 was regarded as statistically significant.
C. elegans worms were grown during 96 or 120 h on regular agar plates or agar plates supplemented with 150 μM CuCl2. The animals were washed off the plates with dH2O, placed on microfuge tubes and subjected to three washes with dH2O, they were then heated 2 times at 100°C for 15 min. All samples were stored at 4°C. To avoid possible metal contaminations, the tubes used to collect the samples were previously treated with 0.5% HNO3 for 24 h, washed with dH2O 3 times and let dry at 20°C. The samples were digested in 200 μl of 12N HNO3 (BDH, ARISTAR) overnight, the next day 200 μl of 1% HNO3 were added. Samples were further diluted (1:10) in 1% HNO3 for analysis by inductive coupled plasma mass spectrometry (ICP-MS). ICP-MS was performed using an UltraMass 700 (Varian Inc., Australia). Tissue samples were prepared in triplicate for all treatments and Cu2+ values for each sample was determined in triplicate. Cu2+ values are mean μg/g weight of each C. elegans sample.
Age-synchronous C. elegans populations were prepared by transferring eggs to a freshly seeded NGM plate. After 96 h, the worms were transferred to K medium (53 mM NaCl, 32 mM KCl) supplemented with different concentrations of CuCl2 (up to 450 μM) on 24 well microtiter plates (15–20 worms/well) for 24 h at 20°C . The number of dead worms was determined by the absence of touch-provoked movement when prodded with a platinum wire. Each independent experiment of a total of three was performed in triplicate.
Lifespan analysis was conducted at 20°C as described previously . For the lifespan assays, 5–10 adult animals were allowed to lay eggs overnight on regular NGM plates seeded with OP50 bacteria. Parents were removed the next day and eggs were allowed to develop at 20°C. L4 larvae were transferred to new regular NGM plates or to 150 μM CuCl2 supplemented plates. This time point was used as day 0 to account for differences in developmental time between strains. The worms were transferred to new plates every day during the first 4–5 days to avoid the mixing of generations. The number of dead individuals was verified every two days. A worm was considered dead when it did not respond when prodded two times with a platinum wire pick. Worms that died with larvae inside (eggs hatched before being laid) or those in which the intestine extruded from the vulva were removed from the sample. Lifespan curves were generated and analyzed with GraphPad PRISM® version 5.0. Log-rank (Mantel-Cox) test was used for statistical analysis of survival curves.
Individual animals of the same age were placed on an 80 μl drop of M9 buffer. After a 2-minute recovery period the worms were recorded for 1.5 minutes and the thrashes counted. A thrash is defined as a change in the direction of bending at the mid body . For the copper treatment experiments, the worms to be assayed were exposed to copper from the embryo (egg) stage in agar plates until the thrashing experiments were performed at 72 h (1 day-old adults), and 168 h later (5 day-old adults).
We thank Dr. Christopher Link (University of Colorado, Boulder, USA) for his generous gift of C. elegans strains and plasmid pCL12 and Dr. Jeremy Nance (New York University, New York, USA) for plasmid pJN254. We also thank Andrea S. Boccardo for technical assistance.
Some nematode strains used in this work were provided by the Caenorhabditis Genetics Center, which is funded by the NIH National Center for Research Resources (NCRR). This work was supported by the International Copper Association (ICA, New York, N.Y.), FONDAP-Biomedicine grant N° 13980001, Millennium Institute of Fundamental and Applied Biology (MIFAB), the Center for Aging and Regeneration (CARE), PFB 12/2007, Base Financing Program for Scientific and Technological Centers of Excellence, CONICYT, the Alzheimer's Association, the NHMRC and the NIA (R01AG12686 to AIB).
- Askanas V, Engel WK: Inclusion-body myositis: a myodegenerative conformational disorder associated with Aβ, protein misfolding, and proteasome inhibition. Neurology. 2006, 66: S39-48.View ArticlePubMedGoogle Scholar
- Masters CL, Multhaup G, Simms G, Pottgiesser J, Martins RN, Beyreuther K: Neuronal origin of a cerebral amyloid: neurofibrillary tangles of Alzheimer's disease contain the same protein as the amyloid of plaque cores and blood vessels. Embo J. 1985, 4: 2757-2763.PubMed CentralPubMedGoogle Scholar
- Askanas V, Engel WK: Inclusion-body myositis and myopathies: different etiologies, possibly similar pathogenic mechanisms. Curr Opin Neurol. 2002, 15: 525-531.View ArticlePubMedGoogle Scholar
- McFerrin J, Engel WK, Askanas V: Impaired innervation of cultured human muscle overexpressing βAPP experimentally and genetically: relevance to inclusion-body myopathies. Neuroreport. 1998, 9: 3201-3205.View ArticlePubMedGoogle Scholar
- Askanas V, Engel WK: Inclusion-body myositis, a multifactorial muscle disease associated with aging: current concepts of pathogenesis. Curr Opin Rheumatol. 2007, 19: 550-559.View ArticlePubMedGoogle Scholar
- Rebolledo DL, Minniti AN, Grez PM, Fadic R, Kohn R, Inestrosa NC: Inclusion body myositis: a view from the Caenorhabditis elegans muscle. Mol Neurobiol. 2008, 38: 178-198.View ArticlePubMedGoogle Scholar
- Selkoe DJ: Alzheimer's disease: genes, proteins, and therapy. Physiol Rev. 2001, 81: 741-766.PubMedGoogle Scholar
- Hori Y, Hashimoto T, Wakutani Y, Urakami K, Nakashima K, Condron MM, Tsubuki S, Saido TC, Teplow DB, Iwatsubo T: The Tottori (D7N) and English (H6R) familial Alzheimer disease mutations accelerate Aβ fibril formation without increasing protofibril formation. J Biol Chem. 2007, 282: 4916-4923.View ArticlePubMedGoogle Scholar
- Johansson AS, Berglind-Dehlin F, Karlsson G, Edwards K, Gellerfors P, Lannfelt L: Physiochemical characterization of the Alzheimer's disease-related peptides Aβ1–42Arctic and Aβ1–42wt. Febs J. 2006, 273: 2618-2630.View ArticlePubMedGoogle Scholar
- Lashuel HA, Hartley DM, Petre BM, Wall JS, Simon MN, Walz T, Lansbury PT: Mixtures of wild-type and a pathogenic (E22G) form of Aβ40 in vitro accumulate protofibrils, including amyloid pores. J Mol Biol. 2003, 332: 795-808.View ArticlePubMedGoogle Scholar
- Murakami K, Irie K, Morimoto A, Ohigashi H, Shindo M, Nagao M, Shimizu T, Shirasawa T: Neurotoxicity and physicochemical properties of Aβ mutant peptides from cerebral amyloid angiopathy: implication for the pathogenesis of cerebral amyloid angiopathy and Alzheimer's disease. J Biol Chem. 2003, 278: 46179-46187.View ArticlePubMedGoogle Scholar
- Nilsberth C, Westlind-Danielsson A, Eckman CB, Condron MM, Axelman K, Forsell C, Stenh C, Luthman J, Teplow DB, Younkin SG, et al: The 'Arctic' APP mutation (E693G) causes Alzheimer's disease by enhanced Aβ protofibril formation. Nat Neurosci. 2001, 4: 887-893.View ArticlePubMedGoogle Scholar
- Cheng IH, Palop JJ, Esposito LA, Bien-Ly N, Yan F, Mucke L: Aggressive amyloidosis in mice expressing human amyloid peptides with the Arctic mutation. Nat Med. 2004, 10: 1190-1192.View ArticlePubMedGoogle Scholar
- Soto C, Castano EM, Frangione B, Inestrosa NC: The α-helical to β-strand transition in the amino-terminal fragment of the amyloid β-peptide modulates amyloid formation. J Biol Chem. 1995, 270: 3063-3067.View ArticlePubMedGoogle Scholar
- Morgan C, Colombres M, Nunez MT, Inestrosa NC: Structure and function of amyloid in Alzheimer's disease. Prog Neurobiol. 2004, 74: 323-349.View ArticlePubMedGoogle Scholar
- Selkoe DJ: The cell biology of β-amyloid precursor protein and presenilin in Alzheimer's disease. Trends Cell Biol. 1998, 8: 447-453.View ArticlePubMedGoogle Scholar
- Herms J, Anliker B, Heber S, Ring S, Fuhrmann M, Kretzschmar H, Sisodia S, Muller U: Cortical dysplasia resembling human type 2 lissencephaly in mice lacking all three APP family members. Embo J. 2004, 23: 4106-4115.PubMed CentralView ArticlePubMedGoogle Scholar
- Lee JY, Cole TB, Palmiter RD, Suh SW, Koh JY: Contribution by synaptic zinc to the gender-disparate plaque formation in human Swedish mutant APP transgenic mice. Proc Natl Acad Sci USA. 2002, 99: 7705-7710.PubMed CentralView ArticlePubMedGoogle Scholar
- Lee JY, Mook-Jung I, Koh JY: Histochemically reactive zinc in plaques of the Swedish mutant β-amyloid precursor protein transgenic mice. J Neurosci. 1999, 19: RC10-PubMedGoogle Scholar
- Lovell MA, Robertson JD, Teesdale WJ, Campbell JL, Markesbery WR: Copper, iron and zinc in Alzheimer's disease senile plaques. J Neurol Sci. 1998, 158: 47-52.View ArticlePubMedGoogle Scholar
- Bush AI, Pettingell WH, Multhaup G, d Paradis M, Vonsattel JP, Gusella JF, Beyreuther K, Masters CL, Tanzi RE: Rapid induction of Alzheimer Aβ amyloid formation by zinc. Science. 1994, 265: 1464-1467.View ArticlePubMedGoogle Scholar
- Cherny RA, Atwood CS, Xilinas ME, Gray DN, Jones WD, McLean CA, Barnham KJ, Volitakis I, Fraser FW, Kim Y, et al: Treatment with a copper-zinc chelator markedly and rapidly inhibits β-amyloid accumulation in Alzheimer's disease transgenic mice. Neuron. 2001, 30: 665-676.View ArticlePubMedGoogle Scholar
- Cherny RA, Legg JT, McLean CA, Fairlie DP, Huang X, Atwood CS, Beyreuther K, Tanzi RE, Masters CL, Bush AI: Aqueous dissolution of Alzheimer's disease Aβ amyloid deposits by biometal depletion. J Biol Chem. 1999, 274: 23223-23228.View ArticlePubMedGoogle Scholar
- Ritchie CW, Bush AI, Mackinnon A, Macfarlane S, Mastwyk M, MacGregor L, Kiers L, Cherny R, Li QX, Tammer A, et al: Metal-protein attenuation with iodochlorhydroxyquin (clioquinol) targeting Aβ amyloid deposition and toxicity in Alzheimer disease: a pilot phase 2 clinical trial. Arch Neurol. 2003, 60: 1685-1691.View ArticlePubMedGoogle Scholar
- Bayer TA, Schafer S, Simons A, Kemmling A, Kamer T, Tepest R, Eckert A, Schussel K, Eikenberg O, Sturchler-Pierrat C, et al: Dietary Cu stabilizes brain superoxide dismutase 1 activity and reduces amyloid Aβ production in APP23 transgenic mice. Proc Natl Acad Sci USA. 2003, 100: 14187-14192.PubMed CentralView ArticlePubMedGoogle Scholar
- Cruz L, Urbanc B, Buldyrev SV, Christie R, Gomez-Isla T, Havlin S, McNamara M, Stanley HE, Hyman BT: Aggregation and disaggregation of senile plaques in Alzheimer disease. Proc Natl Acad Sci USA. 1997, 94: 7612-7616.PubMed CentralView ArticlePubMedGoogle Scholar
- Daigle I, Li C: apl-1, a Caenorhabditis elegans gene encoding a protein related to the human β-amyloid protein precursor. Proc Natl Acad Sci USA. 1993, 90: 12045-12049.PubMed CentralView ArticlePubMedGoogle Scholar
- Fay DS, Fluet A, Johnson CJ, Link CD: In vivo aggregation of β-amyloid peptide variants. J Neurochem. 1998, 71: 1616-1625.View ArticlePubMedGoogle Scholar
- Drake J, Link CD, Butterfield DA: Oxidative stress precedes fibrillar deposition of Alzheimer's disease amyloid β-peptide (1–42) in a transgenic Caenorhabditis elegans model. Neurobiol Aging. 2003, 24: 415-420.View ArticlePubMedGoogle Scholar
- Fonte V, Kapulkin V, Taft A, Fluet A, Friedman D, Link CD: Interaction of intracellular β amyloid peptide with chaperone proteins. Proc Natl Acad Sci USA. 2002, 99: 9439-9444.PubMed CentralView ArticlePubMedGoogle Scholar
- Link CD, Cypser JR, Johnson CJ, Johnson TE: Direct observation of stress response in Caenorhabditis elegans using a reporter transgene. Cell Stress Chaperones. 1999, 4: 235-242.PubMed CentralView ArticlePubMedGoogle Scholar
- Link CD, Taft A, Kapulkin V, Duke K, Kim S, Fei Q, Wood DE, Sahagan BG: Gene expression analysis in a transgenic Caenorhabditis elegans Alzheimer's disease model. Neurobiol Aging. 2003, 24: 397-413.View ArticlePubMedGoogle Scholar
- Wu Y, Wu Z, Butko P, Christen Y, Lambert MP, Klein WL, Link CD, Luo Y: Amyloid-β-induced pathological behaviors are suppressed by Ginkgo biloba extract EGb 761 and ginkgolides in transgenic Caenorhabditis elegans. J Neurosci. 2006, 26: 13102-13113.View ArticlePubMedGoogle Scholar
- De Felice FG, Velasco PT, Lambert MP, Viola K, Fernandez SJ, Ferreira ST, Klein WL: Aβ oligomers induce neuronal oxidative stress through an N-methyl-D-aspartate receptor-dependent mechanism that is blocked by the Alzheimer drug memantine. J Biol Chem. 2007, 282: 11590-11601.View ArticlePubMedGoogle Scholar
- Lacor PN, Buniel MC, Furlow PW, Clemente AS, Velasco PT, Wood M, Viola KL, Klein WL: Aβ oligomer-induced aberrations in synapse composition, shape, and density provide a molecular basis for loss of connectivity in Alzheimer's disease. J Neurosci. 2007, 27: 796-807.View ArticlePubMedGoogle Scholar
- Hammans S: Inclusion Body Myositis. Southampton for the Muscular Dystrophy Campaign. London. 2003, [http://www.muscular-dystrophy.org/information_resources/factsheets/medical_conditions_factsheets/inclusion_body.html]Google Scholar
- Phinney AL, Drisaldi B, Schmidt SD, Lugowski S, Coronado V, Liang Y, Horne P, Yang J, Sekoulidis J, Coomaraswamy J, et al: In vivo reduction of amyloid-β by a mutant copper transporter. Proc Natl Acad Sci USA. 2003, 100: 14193-14198.PubMed CentralView ArticlePubMedGoogle Scholar
- Atwood CS, Moir RD, Huang X, Scarpa RC, Bacarra NM, Romano DM, Hartshorn MA, Tanzi RE, Bush AI: Dramatic aggregation of Alzheimer Aβ by Cu(II) is induced by conditions representing physiological acidosis. J Biol Chem. 1998, 273: 12817-12826.View ArticlePubMedGoogle Scholar
- Opazo C, Huang X, Cherny RA, Moir RD, Roher AE, White AR, Cappai R, Masters CL, Tanzi RE, Inestrosa NC, Bush AI: Metalloenzyme-like activity of Alzheimer's disease β-amyloid. Cu-dependent catalytic conversion of dopamine, cholesterol, and biological reducing agents to neurotoxic H(2)O(2). J Biol Chem. 2002, 277: 40302-40308.View ArticlePubMedGoogle Scholar
- Link CD, Johnson CJ: Reporter transgenes for study of oxidant stress in Caenorhabditis elegans. Methods Enzymol. 2002, 353: 497-505.View ArticlePubMedGoogle Scholar
- Link CD, Johnson CJ, Fonte V, Paupard M, Hall DH, Styren S, Mathis CA, Klunk WE: Visualization of fibrillar amyloid deposits in living, transgenic Caenorhabditis elegans animals using the sensitive amyloid dye, X-34. Neurobiol Aging. 2001, 22: 217-226.View ArticlePubMedGoogle Scholar
- Atwood CS, Scarpa RC, Huang X, Moir RD, Jones WD, Fairlie DP, Tanzi RE, Bush AI: Characterization of copper interactions with alzheimer amyloid β peptides: identification of an attomolar-affinity copper binding site on amyloid β1–42. J Neurochem. 2000, 75: 1219-1233.View ArticlePubMedGoogle Scholar
- Stringham E, Candido E: Transgenic Hsp 16-Lacz Strains of the Soil Nematode Caenorhabditis elegans as Biological Monitors of Environmental Stress. Environmental Toxicology and Chemistry. 1994, 13: 1211-1220.View ArticleGoogle Scholar
- Barsyte D, Lovejoy DA, Lithgow GJ: Longevity and heavy metal resistance in daf-2 and age-1 long-lived mutants of Caenorhabditis elegans. Faseb J. 2001, 15: 627-634.View ArticlePubMedGoogle Scholar
- Candido EP, Jones D: Transgenic Caenorhabditis elegans strains as biosensors. Trends Biotechnol. 1996, 14: 125-129.View ArticlePubMedGoogle Scholar
- Bush AI, Masters CL, Tanzi RE: Copper, β-amyloid, and Alzheimer's disease: tapping a sensitive connection. Proc Natl Acad Sci USA. 2003, 100: 11193-11194.PubMed CentralView ArticlePubMedGoogle Scholar
- Miller KG, Alfonso A, Nguyen M, Crowell JA, Johnson CD, Rand JB: A genetic selection for Caenorhabditis elegans synaptic transmission mutants. Proc Natl Acad Sci USA. 1996, 93: 12593-12598.PubMed CentralView ArticlePubMedGoogle Scholar
- Ackley BD, Kang SH, Crew JR, Suh C, Jin Y, Kramer JM: The basement membrane components nidogen and type XVIII collagen regulate organization of neuromuscular junctions in Caenorhabditis elegans. J Neurosci. 2003, 23: 3577-3587.PubMedGoogle Scholar
- Doi M, Iwasaki K: Regulation of retrograde signaling at neuromuscular junctions by the novel C2 domain protein AEX-1. Neuron. 2002, 33: 249-259.View ArticlePubMedGoogle Scholar
- Cheng IH, Scearce-Levie K, Legleiter J, Palop JJ, Gerstein H, Bien-Ly N, Puolivali J, Lesne S, Ashe KH, Muchowski PJ, Mucke L: Accelerating amyloid-β fibrillization reduces oligomer levels and functional deficits in Alzheimer disease mouse models. J Biol Chem. 2007, 282: 23818-23828.View ArticlePubMedGoogle Scholar
- Oddo S, Caccamo A, Shepherd JD, Murphy MP, Golde TE, Kayed R, Metherate R, Mattson MP, Akbari Y, LaFerla FM: Triple-transgenic model of Alzheimer's disease with plaques and tangles: intracellular Aβ and synaptic dysfunction. Neuron. 2003, 39: 409-421.View ArticlePubMedGoogle Scholar
- Aldridge S: News. In brief: New Alzheimer's endpoints?. Nat Biotechnol. 2008, 26: 482-View ArticlePubMedGoogle Scholar
- Jin Y: C. elegans: A practical approach. 1999, Oxford: Oxford University PressGoogle Scholar
- Nunomura A, Chiba S, Lippa CF, Cras P, Kalaria RN, Takeda A, Honda K, Smith MA, Perry G: Neuronal RNA oxidation is a prominent feature of familial Alzheimer's disease. Neurobiol Dis. 2004, 17: 108-113.View ArticlePubMedGoogle Scholar
- Nunomura A, Perry G, Aliev G, Hirai K, Takeda A, Balraj EK, Jones PK, Ghanbari H, Wataya T, Shimohama S, et al: Oxidative damage is the earliest event in Alzheimer disease. J Neuropathol Exp Neurol. 2001, 60: 759-767.PubMedGoogle Scholar
- Nunomura A, Perry G, Hirai K, Aliev G, Takeda A, Chiba S, Smith MA: Neuronal RNA oxidation in Alzheimer's disease and Down's syndrome. Ann N Y Acad Sci. 1999, 893: 362-364.View ArticlePubMedGoogle Scholar
- Nunomura A, Perry G, Pappolla MA, Friedland RP, Hirai K, Chiba S, Smith MA: Neuronal oxidative stress precedes amyloid-β deposition in Down syndrome. J Neuropathol Exp Neurol. 2000, 59: 1011-1017.PubMedGoogle Scholar
- Cuajungco MP, Faget KY, Huang X, Tanzi RE, Bush AI: Metal chelation as a potential therapy for Alzheimer's disease. Ann N Y Acad Sci. 2000, 920: 292-304.View ArticlePubMedGoogle Scholar
- Hayashi T, Shishido N, Nakayama K, Nunomura A, Smith MA, Perry G, Nakamura M: Lipid peroxidation and 4-hydroxy-2-nonenal formation by copper ion bound to amyloid-β peptide. Free Radic Biol Med. 2007, 43: 1552-1559.View ArticlePubMedGoogle Scholar
- Rae TD, Schmidt PJ, Pufahl RA, Culotta VC, O'Halloran TV: Undetectable intracellular free copper: the requirement of a copper chaperone for superoxide dismutase. Science. 1999, 284: 805-808.View ArticlePubMedGoogle Scholar
- Maynard CJ, Bush AI, Masters CL, Cappai R, Li QX: Metals and amyloid-β in Alzheimer's disease. Int J Exp Pathol. 2005, 86: 147-159.PubMed CentralView ArticlePubMedGoogle Scholar
- Maynard CJ, Cappai R, Volitakis I, Cherny RA, White AR, Beyreuther K, Masters CL, Bush AI, Li QX: Overexpression of Alzheimer's disease amyloid-β opposes the age-dependent elevations of brain copper and iron. J Biol Chem. 2002, 277: 44670-44676.View ArticlePubMedGoogle Scholar
- Owen CA: Uptake of 67Cu by ceruloplasmin in vitro. Proc Soc Exp Biol Med. 1975, 149: 681-682.View ArticlePubMedGoogle Scholar
- Yates CM, Butterworth J, Tennant MC, Gordon A: Enzyme activities in relation to pH and lactate in postmortem brain in Alzheimer-type and other dementias. J Neurochem. 1990, 55: 1624-1630.View ArticlePubMedGoogle Scholar
- Cerpa W, Varela-Nallar L, Reyes AE, Minniti AN, Inestrosa NC: Is there a role for copper in neurodegenerative diseases?. Mol Aspects Med. 2005, 26: 405-420.View ArticlePubMedGoogle Scholar
- Cerpa WF, Barria MI, Chacon MA, Suazo M, Gonzalez M, Opazo C, Bush AI, Inestrosa NC: The N-terminal copper-binding domain of the amyloid precursor protein protects against Cu2+ neurotoxicity in vivo. Faseb J. 2004, 18: 1701-1703.PubMedGoogle Scholar
- Zou K, Gong JS, Yanagisawa K, Michikawa M: A novel function of monomeric amyloid β-protein serving as an antioxidant molecule against metal-induced oxidative damage. J Neurosci. 2002, 22: 4833-4841.PubMedGoogle Scholar
- Sparks DL, Schreurs BG: Trace amounts of copper in water induce β-amyloid plaques and learning deficits in a rabbit model of Alzheimer's disease. Proc Natl Acad Sci USA. 2003, 100: 11065-11069.PubMed CentralView ArticlePubMedGoogle Scholar
- Link CD: Expression of human β-amyloid peptide in transgenic Caenorhabditis elegans. Proc Natl Acad Sci USA. 1995, 92: 9368-9372.PubMed CentralView ArticlePubMedGoogle Scholar
- Brenner S: The genetics of Caenorhabditis elegans. Genetics. 1974, 77: 71-94.PubMed CentralPubMedGoogle Scholar
- Lewis JA, Fleming JT: Basic culture methods. Methods Cell Biol. 1995, 48: 3-29.View ArticlePubMedGoogle Scholar
- Praitis V, Casey E, Collar D, Austin J: Creation of low-copy integrated transgenic lines in Caenorhabditis elegans. Genetics. 2001, 157: 1217-1226.PubMed CentralPubMedGoogle Scholar
- Finney M, Ruvkun G: The unc-86 gene product couples cell lineage and cell identity in C. elegans. Cell. 1990, 63: 895-905.View ArticlePubMedGoogle Scholar
- Kenyon C, Chang J, Gensch E, Rudner A, Tabtiang R: A C. elegans mutant that lives twice as long as wild type. Nature. 1993, 366: 461-464.View ArticlePubMedGoogle Scholar
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